{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T14:27:31Z","timestamp":1785421651148,"version":"3.56.0"},"reference-count":50,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T00:00:00Z","timestamp":1777334400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004704","name":"National Research Council of Thailand","doi-asserted-by":"publisher","award":["N41A661179"],"award-info":[{"award-number":["N41A661179"]}],"id":[{"id":"10.13039\/501100004704","id-type":"DOI","asserted-by":"publisher"}]},{"award":["N41A661179"],"award-info":[{"award-number":["N41A661179"]}],"id":[{"id":"https:\/\/ror.org\/018wfhg78","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010034","name":"Walailak University","doi-asserted-by":"publisher","award":["CGS-RF-2024\/05"],"award-info":[{"award-number":["CGS-RF-2024\/05"]}],"id":[{"id":"10.13039\/501100010034","id-type":"DOI","asserted-by":"publisher"}]},{"award":["CGS-RF-2024\/05"],"award-info":[{"award-number":["CGS-RF-2024\/05"]}],"id":[{"id":"https:\/\/ror.org\/04b69g067","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>Globally, the rise in MDR P. aeruginosa infections poses a serious threat to public health, as these strains frequently exhibit extensive resistance to conventional antibiotics, prompting interest in bacteriophages as alternative treatments. In this study, we isolated and characterized a lytic P. aeruginosa phage, vB_Pae_YuaWU01, from hospital sewage in southern Thailand. Morphological analysis revealed Siphovirus-like characteristics. The phage demonstrated efficient host adsorption, with approximately 85.9% of particles attached within 15 min, and exhibited a latent period of 50 min with a burst size of 17.2 PFU\/cell. It showed strong lytic activity, consistently suppressing bacterial growth without no regrowth observed over 72 h. Notably, the phage significantly inhibited biofilm formation by up to 59.9% and reduced pre-established biofilms by 39.78% at the highest tested concentration (109 PFU\/mL). Genome analysis revealed a 61,824 bp double-stranded DNA genome with 64.48% GC content and 88 predicted genes. Bioinformatic analysis suggests that the genome is organized into structural, replication, and lysis modules. Importantly, no toxin, antimicrobial resistance, lysogeny, or tRNA genes were identified, suggesting a favorable safety profile. The phage was classified within the genus Yuavirus, showing 97.4% genomic similarity to Sphaerotilus phage SN1, which infects a different host strain. The findings highlight its potential as a genetically safe therapeutic agent; however, its limited host range indicates that it may be best positioned as a strategic component of phage cocktails or as a synergistic partner with antibiotics to maximize therapeutic efficacy.<\/jats:p>","DOI":"10.3390\/life16050734","type":"journal-article","created":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T12:31:56Z","timestamp":1777465916000},"page":"734","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Isolation and Genomic Characterization of Lytic Caudoviricetes Bacteriophage vB_Pae_YuaWU01 Targeting Multidrug-Resistant Pseudomonas aeruginosa from Hospital Sewage in Southern Thailand"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-7401-9966","authenticated-orcid":false,"given":"Dechawat","family":"Wongprot","sequence":"first","affiliation":[{"name":"College of Graduate Studies, Walailak University, Nakhon Si Thammarat 80160, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benyapa","family":"Prakit","sequence":"additional","affiliation":[{"name":"College of Graduate Studies, Walailak University, Nakhon Si Thammarat 80160, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7419-0116","authenticated-orcid":false,"given":"Morteza","family":"Saki","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1403-1628","authenticated-orcid":false,"given":"Chonticha","family":"Romyasamit","sequence":"additional","affiliation":[{"name":"Department of Medical Technology, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80160, Thailand"},{"name":"Research Center in Tropical Pathobiology, Walailak University, Thasala District, Nakhon Si Thammarat 80160, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Vaitekenas, A., Tai, A.S., Ramsay, J.P., Stick, S.M., and Kicic, A. 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