{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T14:45:45Z","timestamp":1785422745346,"version":"3.56.0"},"reference-count":53,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T00:00:00Z","timestamp":1779753600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004829","name":"Science & Technology Department of Sichuan Province","doi-asserted-by":"crossref","award":["2025ZNSFSC0205"],"award-info":[{"award-number":["2025ZNSFSC0205"]}],"id":[{"id":"10.13039\/501100004829","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004829","name":"Science & Technology Department of Sichuan Province","doi-asserted-by":"crossref","award":["2022NSFSC1133"],"award-info":[{"award-number":["2022NSFSC1133"]}],"id":[{"id":"10.13039\/501100004829","id-type":"DOI","asserted-by":"crossref"}]},{"award":["2025ZNSFSC0205"],"award-info":[{"award-number":["2025ZNSFSC0205"]}],"id":[{"id":"https:\/\/ror.org\/04323m874","id-type":"ROR","asserted-by":"publisher"}]},{"award":["2022NSFSC1133"],"award-info":[{"award-number":["2022NSFSC1133"]}],"id":[{"id":"https:\/\/ror.org\/04323m874","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Sichuan Province Regional Innovation Cooperation Project","award":["2024YFHZ0102"],"award-info":[{"award-number":["2024YFHZ0102"]}]},{"name":"Scientific Research Initiation Project of Mianyang Teachers\u2019 College","award":["QD2020A12"],"award-info":[{"award-number":["QD2020A12"]}]},{"name":"Mianyang Science and Technology Program","award":["2023ZYDF076"],"award-info":[{"award-number":["2023ZYDF076"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>Ocimum basilicum is widely used as both a culinary and medicinal plant; however, its nutritional metabolite composition, functional relevance, and underlying regulatory mechanisms remain incompletely characterized. To systematically profile nutritional metabolites and explore their potential biological relevance in O. basilicum, an integrative multi-omics strategy that combined UPLC-MS\/MS-based metabolomics, transcriptomics, network pharmacology, and molecular docking was deployed herein. A total of 443 nutritional metabolites across four accessions were identified, including vitamins, saccharides, amino acids and lipids. Of these, Vitamin A1 (retinol) and N-acetyl-L-tryptophan were found to be important metabolites that could have functional significance. Predictive network pharmacology and molecular docking analyses suggested potential in silico interactions between these metabolites and disease-associated targets, including ESR2 and MAPK1; these findings await experimental validation. Transcriptomic analysis also showed that genes involved in Vitamin A1 biosynthesis (PSY, LCYB) were expressed, and the expression patterns of the genes were validated by the qPCR analysis, in which expression level was largely consistent with the transcriptome results. Specifically, the accessions G083 and G082 showed high values of Vitamin A1 and N-acetyl-L-tryptophan, respectively, suggesting that they may also be interesting germplasm for functional food innovation and the development of nutraceuticals. Overall, this study offers a comprehensive multi-omics dataset and mechanistic insights that can help in the targeted use of O. basilicum for nutritional and functional applications.<\/jats:p>","DOI":"10.3390\/life16060890","type":"journal-article","created":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T08:16:08Z","timestamp":1779783368000},"page":"890","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrative Multi-Omics Analysis Reveals Nutritional Metabolite Diversity and Regulatory Mechanisms in Ocimum basilicum"],"prefix":"10.3390","volume":"16","author":[{"given":"Yuanyuan","family":"Zhang","sequence":"first","affiliation":[{"name":"College of Biology and Pharmacy, Mianyang Teachers\u2019 College, Mianyang 621000, China"},{"name":"Key Laboratory for Quality Control and Evaluation of Traditional Chinese Medicine in Mianyang, Mianyang Teachers\u2019 College, Mianyang 621000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manman","family":"Xu","sequence":"additional","affiliation":[{"name":"Forest Ecology and Conservation in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province, Engineering Research Center for Forest and Grassland Disaster Prevention and Reduction at Mianyang Teachers\u2019 College of Sichuan Provincial Department of Education, School of Life Sciences, Mianyang Teachers\u2019 College, Mianyang 621000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zizuo","family":"Miseme","sequence":"additional","affiliation":[{"name":"College of Biology and Pharmacy, Mianyang Teachers\u2019 College, Mianyang 621000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiqi","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Biology and Pharmacy, Mianyang Teachers\u2019 College, Mianyang 621000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangrong","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Biology and Pharmacy, Mianyang Teachers\u2019 College, Mianyang 621000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6160-732X","authenticated-orcid":false,"given":"Cong","family":"Zhao","sequence":"additional","affiliation":[{"name":"School of Environmental Science and Engineering, Southwest Jiaotong University, Chengdu 611756, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujian","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Geography and Environment, Mianyang Teachers\u2019 College, Mianyang 621000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1153-5495","authenticated-orcid":false,"given":"Jingtian","family":"Yang","sequence":"additional","affiliation":[{"name":"Forest Ecology and Conservation in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province, Engineering Research Center for Forest and Grassland Disaster Prevention and Reduction at Mianyang Teachers\u2019 College of Sichuan Provincial Department of Education, School of Life Sciences, Mianyang Teachers\u2019 College, Mianyang 621000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,5,26]]},"reference":[{"key":"ref_1","first-page":"1209","article-title":"First Report of Colletotrichum Siamense Causing Blossom Blight on Thai Basil (Ocimum basilicum) in Malaysia","volume":"105","author":"Ismail","year":"2021","journal-title":"Plant Dis."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Zhou, Y., Ma, G., Li, W., Xie, L., Zhan, S., Yao, X., Zuo, Z., and Tian, D. 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