{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T10:04:22Z","timestamp":1783937062050,"version":"3.55.0"},"reference-count":43,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T00:00:00Z","timestamp":1783900800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008227","name":"University of South Africa","doi-asserted-by":"publisher","award":["WIR-2024-1609"],"award-info":[{"award-number":["WIR-2024-1609"]}],"id":[{"id":"10.13039\/501100008227","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>Antimicrobial resistance in various microorganisms and opportunistic pathogens associated with HIV\/AIDS poses a serious threat to human life and healthcare systems worldwide. Different forms of cancer are likely to arise in immunocompromised patients. The antimicrobial and anticancer effects of methanol extract and fractions from Raphionacme hirsuta have been investigated. The carbon tetrachloride fraction showed a remarkably low minimum inhibitory concentration (MIC) of 0.02 mg\/mL against Escherichia coli, Mycoplasma hominis, and Cryptococcus neoformans, while the n-hexane fraction showed a similar MIC against C. neoformans. Furthermore, the carbon tetrachloride fraction exhibited promising IC50 values of 18.21 and 25.22 \u00b5g\/mL against HeLa and MCF-7 cancerous cell lines, respectively. The fraction was subjected to GC-TOF-MS and yielded four major compounds, including 7,9-Di-tert-butyl-1-oxaspiro (4,5) deca-6,9-diene-2,8-dione (5.322%), Hexanedial (3.691%), 4-(4-tert-Butylphenyl)-1,3-thiazol-2-ylamine (3.329%), and Di-n-decylsulfone (3.201%). These substances could potentially account for the plant species\u2019 initial biological activity, which is why it is necessary to investigate their in vivo actions. The gummy extract had less biological activity than the fractions. To the best of our knowledge, this is the first study to report the antimicrobial and anticancer activities, as well as the phytochemistry, of the plant species.<\/jats:p>","DOI":"10.3390\/life16071154","type":"journal-article","created":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T08:53:58Z","timestamp":1783932838000},"page":"1154","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["In Vitro Antimicrobial and Cytotoxic Effects of Solvent-Fractionated Extracts from Raphionacme hirsuta (E.Mey.) R.A.Dyer (Apocynaceae) Bulbs"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4167-9294","authenticated-orcid":false,"given":"Nkoana I.","family":"Mongalo","sequence":"first","affiliation":[{"name":"College of Agriculture and Environmental Sciences Laboratories, University of South Africa, Private Bag X6, Florida 1710, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0001-1661","authenticated-orcid":false,"given":"Maropeng V.","family":"Raletsena","sequence":"additional","affiliation":[{"name":"College of Agriculture and Environmental Sciences Laboratories, University of South Africa, Private Bag X6, Florida 1710, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nontokozo","family":"Magwaza","sequence":"additional","affiliation":[{"name":"Institute for Nanotechnology and Water Sustainability, College of Science, Engineering and Technology, University of South Africa, Johannesburg 1710, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Perpetua","family":"Modjadji","sequence":"additional","affiliation":[{"name":"Department of Public Health, School of Health Care Sciences, Sefako Makgatho Health Sciences University, 1 Molotlegi Street, Ga-Rankuwa 0208, South Africa"},{"name":"Non-Communicable Diseases Research Unit, South African Medical Research Council, Tygerberg, Cape Town 7505, South Africa"},{"name":"Department of Life and Consumer Sciences, College of Agriculture and Environmental Sciences, University of South Africa, Florida Campus, Johannesburg 1709, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1016\/j.jep.2008.05.038","article-title":"Antimicrobial activity of South African medicinal plants","volume":"119","year":"2008","journal-title":"J. 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