{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T16:17:15Z","timestamp":1785428235581,"version":"3.56.0"},"reference-count":70,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2026,7,18]],"date-time":"2026-07-18T00:00:00Z","timestamp":1784332800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005307","name":"Directorate General for Scientific Research and Technological Development","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100005307","id-type":"DOI","asserted-by":"crossref"}]},{"id":[{"id":"https:\/\/ror.org\/04jpfrv51","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>This study characterized six Enterococcus faecium strains isolated from Algerian camel milk to assess their molecular features and antimicrobial potential as food-associated isolates. Identification was performed using phenotypic tests, matrix-assisted laser desorption\/ionization time-of-flight mass spectrometry (MALDI-TOF MS), and 16S rRNA gene sequencing. Strain diversity was assessed by repetitive sequence-based polymerase chain reaction (rep-PCR), including (GTG)5-PCR, BOX-PCR, and enterobacterial repetitive intergenic consensus PCR (ERIC-PCR). Polymerase chain reaction (PCR) screening for selected virulence-associated and antimicrobial resistance genes showed that the two selected isolates were negative for the targeted markers, indicating a preliminary molecular profile of interest but not allowing definitive safety conclusions without whole-genome sequencing and phenotypic antimicrobial susceptibility testing. Both strains showed antimicrobial activity against Gram-positive and Gram-negative indicators. Strain 9 showed notable inhibition against Klebsiella pneumoniae subsp. pneumoniae ATCC 13883, with an inhibition zone of 23.4 \u00b1 2.3 mm using native supernatant, which increased after concentration. Trypsin treatment abolished activity, supporting the proteinaceous nature of the inhibitory compounds. The enterocin genes entA and entB were detected in strains 9 and 13, indicating genetic potential only. Further genomic, safety, and functional investigations are required.<\/jats:p>","DOI":"10.3390\/life16071190","type":"journal-article","created":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T00:47:32Z","timestamp":1784508452000},"page":"1190","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Preliminary Molecular Characterization and Antimicrobial Activity of Enterococcus faecium Strains Isolated from Algerian Camel Milk"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5204-8522","authenticated-orcid":false,"given":"Yasmine","family":"Saidi","sequence":"first","affiliation":[{"name":"Genomics Technology Platform, Higher School of Biological Sciences of Oran, BP 1042 Saim Mohamed, Cit\u00e9 Emir Abdelkader (Ex-INESSMO), Oran 31000, Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed","family":"Merzoug","sequence":"additional","affiliation":[{"name":"Genomics Technology Platform, Higher School of Biological Sciences of Oran, BP 1042 Saim Mohamed, Cit\u00e9 Emir Abdelkader (Ex-INESSMO), Oran 31000, Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-4876-4746","authenticated-orcid":false,"given":"Chaima\u00e0 Naila","family":"Brakna","sequence":"additional","affiliation":[{"name":"Genomics Technology Platform, Higher School of Biological Sciences of Oran, BP 1042 Saim Mohamed, Cit\u00e9 Emir Abdelkader (Ex-INESSMO), Oran 31000, Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keltoum","family":"Bendida","sequence":"additional","affiliation":[{"name":"Genomics Technology Platform, Higher School of Biological Sciences of Oran, BP 1042 Saim Mohamed, Cit\u00e9 Emir Abdelkader (Ex-INESSMO), Oran 31000, Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soheir","family":"Bouzidi","sequence":"additional","affiliation":[{"name":"Genomics Technology Platform, Higher School of Biological Sciences of Oran, BP 1042 Saim Mohamed, Cit\u00e9 Emir Abdelkader (Ex-INESSMO), Oran 31000, Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-7677-4897","authenticated-orcid":false,"given":"Marwa","family":"Aireche","sequence":"additional","affiliation":[{"name":"Genomics Technology Platform, Higher School of Biological Sciences of Oran, BP 1042 Saim Mohamed, Cit\u00e9 Emir Abdelkader (Ex-INESSMO), Oran 31000, Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hayet","family":"Messaoui","sequence":"additional","affiliation":[{"name":"Genomics Technology Platform, Higher School of Biological Sciences of Oran, BP 1042 Saim Mohamed, Cit\u00e9 Emir Abdelkader (Ex-INESSMO), Oran 31000, Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-3777-7505","authenticated-orcid":false,"given":"Amaria Ilhem","family":"Hammadi","sequence":"additional","affiliation":[{"name":"Genomics Technology Platform, Higher School of Biological Sciences of Oran, BP 1042 Saim Mohamed, Cit\u00e9 Emir Abdelkader (Ex-INESSMO), Oran 31000, Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-7769-3577","authenticated-orcid":false,"given":"Yasmine Zohra","family":"Zater","sequence":"additional","affiliation":[{"name":"Genomics Technology Platform, Higher School of Biological Sciences of Oran, BP 1042 Saim Mohamed, Cit\u00e9 Emir Abdelkader (Ex-INESSMO), Oran 31000, Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hadjer","family":"Bouderbala","sequence":"additional","affiliation":[{"name":"Genomics Technology Platform, Higher School of Biological Sciences of Oran, BP 1042 Saim Mohamed, Cit\u00e9 Emir Abdelkader (Ex-INESSMO), Oran 31000, Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6377-3305","authenticated-orcid":false,"given":"Svetoslav Dimitrov","family":"Todorov","sequence":"additional","affiliation":[{"name":"ProBacLab, Laborat\u00f3rio de Microbiologia de Alimentos, Departamento de Alimentos e Nutri\u00e7\u00e3o Experimental, Food Research Center, Faculdade de Ci\u00eancias Farmac\u00eauticas, Universidade de S\u00e3o Paulo, S\u00e3o Paulo 05508-000, SP, Brazil"},{"name":"Department of General Hygiene, I.M. Sechenov First Moscow State Medical University, Trubetskaya St., Bldg. 8\/2, Moscow 119435, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3253-7414","authenticated-orcid":false,"given":"Djamel","family":"Saidi","sequence":"additional","affiliation":[{"name":"Genomics Technology Platform, Higher School of Biological Sciences of Oran, BP 1042 Saim Mohamed, Cit\u00e9 Emir Abdelkader (Ex-INESSMO), Oran 31000, Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Park, Y.W., and Haenlein, G.F.W. (2006). Camel Milk. 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