{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T13:00:10Z","timestamp":1785157210045,"version":"3.55.0"},"reference-count":63,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T00:00:00Z","timestamp":1785110400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology of Taiwan","doi-asserted-by":"publisher","award":["MOST107-2320-B-038-024-MY3"],"award-info":[{"award-number":["MOST107-2320-B-038-024-MY3"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>Wolfiporia extensa (Peck) Ginns (WE) is a fungus widely used in Traditional Chinese Medicine. Its four medicinal parts, Poria (WP), Rubra Poria (RP), Poriae Cutis (PC), and Poria Cum Pini Radix (PPR), are prescribed for distinct therapeutic purposes; however, their quality standards and pharmacological differences remain unclear. This study established reliable quality markers and compared the neuroprotective activities of four medicinal parts of WE. Seven triterpenoids were quantified using validated high-performance liquid chromatography (HPLC) assays, while monosaccharide profiles were analyzed using 1-phenyl-3-methyl-5-pyrazolone-HPLC. The International Commission on Illumination L*a*b* colorimetry and chemometric models were used for authentication. The neuroprotective effects of aqueous extracts were assessed in SH-SY5Y, BV-2, and HOG cell models. Network pharmacology was used to predict compound\u2013target pathway relationships. The content of poricoic acid A (PAA) was the highest in PC; the content of pachymic acid (PA) was the highest in RP; whereas dehydrotumulosic acid (DTUA) was enriched in WP. WP demonstrated the highest mannose and galactose content. The water extract of WP reduced H2O2-induced cytotoxicity in SH-SY5Y cells, whereas the PC extract alleviated L-\u03b1-lysophosphatidylcholine-induced injury in human oligodendroglioma cells. Network analysis identified PA, PAA, and poricoic acid B (PAB) as candidate bioactive compounds involved in neuroprotective effects. The PA\/PAB ratios of \u226510, 1\u20139, and \u22641 were used to identify WP, RP, and PC, respectively. L* values of &gt;80, 55\u201379, and &lt;55 effectively discriminated WP, RP, and PC, respectively. This integrative study identified chemical and functional quality markers and demonstrated distinct neuroprotective profiles in different parts of WE, supporting their standardized and rational application in traditional medicine and nutraceuticals.<\/jats:p>","DOI":"10.3390\/life16081241","type":"journal-article","created":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T11:54:26Z","timestamp":1785153266000},"page":"1241","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrated Chemical Profiling and Network Pharmacology Establish a Quality Evaluation Framework for the Four Medicinal Parts of Wolfiporia extensa (Peck) Ginns"],"prefix":"10.3390","volume":"16","author":[{"given":"Shun-Xin","family":"Deng","sequence":"first","affiliation":[{"name":"Ph.D. Program in Clinical Drug Development of Herbal Medicine, Taipei Medical University, Taipei 11031, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2407-6782","authenticated-orcid":false,"given":"Lih-Geeng","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Biopharmaceuticals, College of Life Sciences, National Chiayi University, Chiayi 600355, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0363-5987","authenticated-orcid":false,"given":"Shih-Yi","family":"Hsiung","sequence":"additional","affiliation":[{"name":"School of Pharmacy, College of Pharmacy, Taipei Medical University, Taipei 11031, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vinh Tuyen T.","family":"Le","sequence":"additional","affiliation":[{"name":"Ph.D. Program in Clinical Drug Development of Herbal Medicine, Taipei Medical University, Taipei 11031, Taiwan"},{"name":"Department of Pharmacognosy\u2014Traditional Pharmacy\u2014Pharmaceutical Botany, College of Pharmacy, Can Tho University of Medicine and Pharmacy, Can Tho 941, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9416-5468","authenticated-orcid":false,"given":"Chia-Jung","family":"Lee","sequence":"additional","affiliation":[{"name":"Ph.D. Program in Clinical Drug Development of Herbal Medicine, Taipei Medical University, Taipei 11031, Taiwan"},{"name":"Graduate Institute of Pharmacognosy, Taipei Medical University, Taipei 11031, Taiwan"},{"name":"Traditional Herbal Medicine Research Center, Taipei Medical University Hospital, Taipei 11031, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yves S. Y.","family":"Hsieh","sequence":"additional","affiliation":[{"name":"Ph.D. Program in Clinical Drug Development of Herbal Medicine, Taipei Medical University, Taipei 11031, Taiwan"},{"name":"School of Pharmacy, College of Pharmacy, Taipei Medical University, Taipei 11031, Taiwan"},{"name":"Graduate Institute of Pharmacognosy, Taipei Medical University, Taipei 11031, Taiwan"},{"name":"Traditional Herbal Medicine Research Center, Taipei Medical University Hospital, Taipei 11031, Taiwan"},{"name":"Division of Glycoscience, Department of Chemistry, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, AlbaNova University Centre, SE10691 Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ching-Chiung","family":"Wang","sequence":"additional","affiliation":[{"name":"Ph.D. Program in Clinical Drug Development of Herbal Medicine, Taipei Medical University, Taipei 11031, Taiwan"},{"name":"School of Pharmacy, College of Pharmacy, Taipei Medical University, Taipei 11031, Taiwan"},{"name":"Graduate Institute of Pharmacognosy, Taipei Medical University, Taipei 11031, Taiwan"},{"name":"Traditional Herbal Medicine Research Center, Taipei Medical University Hospital, Taipei 11031, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/bs.irn.2017.02.006","article-title":"Treatment of insomnia with traditional chinese herbal medicine","volume":"135","author":"Singh","year":"2017","journal-title":"Int. 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