{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T13:23:18Z","timestamp":1787664198347,"version":"build-2736575974"},"reference-count":73,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T00:00:00Z","timestamp":1787616000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Science, Technological Development and Innovation of the Republic of Serbia","award":["451\u201303\u201333\/2026\u201303\/200007"],"award-info":[{"award-number":["451\u201303\u201333\/2026\u201303\/200007"]}]},{"award":["451\u201303\u201333\/2026\u201303\/200007"],"award-info":[{"award-number":["451\u201303\u201333\/2026\u201303\/200007"]}],"id":[{"id":"https:\/\/ror.org\/01znas443","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>Cryptic biodiversity presents an ongoing challenge to the accuracy and effectiveness of biodiversity assessment and conservation. To address this, we developed a non-lethal genetic monitoring framework for the subterranean rodent, European lesser blind mole rat (BMR), Nannospalax leucodon species complex, which encompasses multiple chromosomally diversified and reproductively isolated cryptic-species and subspecies. Our workflow combines species and sex detection through: karyotyping, Inter-Simple Sequence Repeat (ISSR) PCR profiling, and Sry-based sex determination of 33 individual samples from five BMR cryptic-species collected at 25 localities in Serbia. As conventional karyotyping protocols for BMR required animal sacrifice, we developed the first non-lethal fibroblast culture-based karyotyping approach from BMR skin biopsy, producing high-quality metaphase chromosomes across five cryptic-species. Among twelve tested ISSR primers, three yielded reproducible, species-specific DNA profiles that resolved four of the five cryptic-species. The Sry assay accurately determined the sex of all examined individuals. Our findings demonstrate that integrating fibroblast culture-based karyotyping, with fast, cost-effective ISSR-PCR species identification, and Sry-based sex determination, provides a reliable approach for identifying and monitoring cryptic BMR species without sacrificing individuals. This framework has potential applications in conservation programmes and contributes to an integrative taxonomy approach essential for the study and protection of other cryptic taxa.<\/jats:p>","DOI":"10.3390\/life16091408","type":"journal-article","created":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T12:57:17Z","timestamp":1787662637000},"page":"1408","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Methodological Perspectives on Cryptic Species Monitoring: Insights from Strictly Protected Lesser Blind Mole Rat"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1669-9341","authenticated-orcid":false,"given":"Marko","family":"\u0110oki\u0107","sequence":"first","affiliation":[{"name":"Department of Genetic Research, Institute for Biological Research \u201cSini\u0161a Stankovi\u0107\u201d\u2014National Institute of Republic of Serbia, University of Belgrade, 11108 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5070-7192","authenticated-orcid":false,"given":"Vida","family":"Joji\u0107","sequence":"additional","affiliation":[{"name":"Department of Genetic Research, Institute for Biological Research \u201cSini\u0161a Stankovi\u0107\u201d\u2014National Institute of Republic of Serbia, University of Belgrade, 11108 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-8238-1213","authenticated-orcid":false,"given":"Pavle","family":"Luki\u0107","sequence":"additional","affiliation":[{"name":"Department of Genetic Research, Institute for Biological Research \u201cSini\u0161a Stankovi\u0107\u201d\u2014National Institute of Republic of Serbia, University of Belgrade, 11108 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9195-9767","authenticated-orcid":false,"given":"Nata\u0161a","family":"Bari\u0161i\u0107 Klisari\u0107","sequence":"additional","affiliation":[{"name":"Department of Evolutionary Biology, Institute for Biological Research \u201cSini\u0161a Stankovi\u0107\u201d\u2014National Institute of Republic of Serbia, University of Belgrade, 11108 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3033-1658","authenticated-orcid":false,"given":"Aleksandra","family":"Penezi\u0107","sequence":"additional","affiliation":[{"name":"Faculty of Biology, University of Belgrade, 11158 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9365-2993","authenticated-orcid":false,"given":"Vanja","family":"Bugarski-Stanojevi\u0107","sequence":"additional","affiliation":[{"name":"Department of Genetic Research, Institute for Biological Research \u201cSini\u0161a Stankovi\u0107\u201d\u2014National Institute of Republic of Serbia, University of Belgrade, 11108 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,25]]},"reference":[{"key":"ref_1","unstructured":"Secretariat of the Convention on Biological Diversity (2001). Handbook of the Convention on Biological Diversity, Earthscan."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2457","DOI":"10.1007\/s10531-019-01804-w","article-title":"The Seventh Plenary of the Intergovernmental Platform for Biodiversity and Ecosystem Services (IPBES-7): A Global Assessment and a Reshaping of IPBES","volume":"28","author":"Bridgewater","year":"2019","journal-title":"Biodivers. Conserv."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Casetta, E., Marques da Silva, J., and Vecchi, D. (2019). From Assessing to Conserving Biodiversity. Conceptual and Practical Challenges, Springer.","DOI":"10.1007\/978-3-030-10991-2"},{"key":"ref_4","unstructured":"Begon, M., and Townsend, C.R. (2020). Ecology: From Individuals to Ecosystems, John Wiley & Sons."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/j.tree.2006.11.004","article-title":"Cryptic Species as a Window on Diversity and Conservation","volume":"22","author":"Bickford","year":"2007","journal-title":"Trends Ecol. Evol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1093\/zoolinnean\/zlaa099","article-title":"Addressing the Linnean Shortfall in a Cryptic Species Complex","volume":"192","author":"Walters","year":"2021","journal-title":"Zool. J. Linn. Soc."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/j.tree.2017.11.007","article-title":"Finding Evolutionary Processes Hidden in Cryptic Species","volume":"33","author":"Struck","year":"2018","journal-title":"Trends Ecol. Evol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1111\/brv.13139","article-title":"Cryptic Species Conservation: A Review","volume":"100","author":"Hending","year":"2025","journal-title":"Biol. Rev."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.ejsobi.2018.03.004","article-title":"Why Are We Blind to Cryptic Species? Lessons from the Eyeless","volume":"86","author":"Novo","year":"2018","journal-title":"Eur. J. Soil. Biol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1111\/mec.14486","article-title":"Cryptic Species as a Window into the Paradigm Shift of the Species Concept","volume":"27","author":"Robinson","year":"2018","journal-title":"Mol. Ecol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1806","DOI":"10.1098\/rspb.2011.1653","article-title":"High Levels of Cryptic Species Diversity Uncovered in Amazonian Frogs","volume":"279","author":"Funk","year":"2012","journal-title":"Proc. R. Soc. B Biol. Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"6732","DOI":"10.1038\/s41598-019-42297-5","article-title":"Multilevel Fine-Scale Diversity Challenges the \u2018Cryptic Species\u2019 Concept","volume":"9","author":"Korshunova","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1016\/S0169-5347(00)01876-0","article-title":"Considering Evolutionary Processes in Conservation Biology","volume":"15","author":"Crandall","year":"2000","journal-title":"Trends Ecol. Evol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1016\/S0169-5347(03)00184-8","article-title":"Delimiting Species: A Renaissance Issue in Systematic Biology","volume":"18","author":"Sites","year":"2003","journal-title":"Trends Ecol. Evol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"879","DOI":"10.1080\/10635150701701083","article-title":"Species Concepts and Species Delimitation","volume":"56","year":"2007","journal-title":"Syst. Biol."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Padial, J.M., Miralles, A., De La Riva, I., and Vences, M. (2010). The Integrative Future of Taxonomy. Front. Zool., 7.","DOI":"10.1186\/1742-9994-7-16"},{"key":"ref_17","unstructured":"International Union for Conservation of Nature (IUCN) (2017). The IUCN Red List of Threatened SpeciesTM Strategic Plan 2017\u20132020, IUCN."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1111\/1755-0998.12447","article-title":"A Nonlethal Sampling Method to Obtain, Generate and Assemble Whole Blood Transcriptomes from Small, Wild Mammals","volume":"16","author":"Huang","year":"2016","journal-title":"Mol. Ecol. Resour."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1038\/s41598-023-27512-8","article-title":"Sampling Environmental DNA from Trees and Soil to Detect Cryptic Arboreal Mammals","volume":"13","author":"Allen","year":"2023","journal-title":"Sci. Rep."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1007\/s10531-023-02728-2","article-title":"Environmental DNA from Soil Reveals the Presence of a \u201cLost\u201d Afrotherian Species","volume":"33","author":"Mynhardt","year":"2024","journal-title":"Biodivers. Conserv."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"119","DOI":"10.3897\/rethinkingecology.4.32751","article-title":"Poor Implementation of Non-Invasive Sampling in Wildlife Genetics Studies","volume":"4","author":"Zemanova","year":"2019","journal-title":"Rethink. Ecol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1789","DOI":"10.1111\/j.1365-294X.2012.05542.x","article-title":"Environmental DNA","volume":"21","author":"Taberlet","year":"2012","journal-title":"Mol. Ecol."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Zemanova, M.A. (2021). Noninvasive Genetic Assessment Is an Effective Wildlife Research Tool When Compared with Other Approaches. Genes, 12.","DOI":"10.3390\/genes12111672"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.ympev.2014.03.015","article-title":"Rapid Chromosomal Evolution in a Morphologically Cryptic Radiation","volume":"77","author":"Mills","year":"2014","journal-title":"Mol. Phylogenet. Evol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/mam.12009","article-title":"Sympatric Sister Species in Rodents Are More Chromosomally Differentiated than Allopatric Ones: Implications for the Role of Chromosomal Rearrangements in Speciation: Chromosomal Speciation in Rodents","volume":"44","author":"Castiglia","year":"2014","journal-title":"Mammal Rev."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1093\/sysbio\/syq004","article-title":"A Network Approach to Study Karyotypic Evolution: The Chromosomal Races of the Common Shrew (Sorex araneus) and House Mouse (Mus musculus) as Model Systems","volume":"59","author":"White","year":"2010","journal-title":"Syst. Biol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"249","DOI":"10.25225\/fozo.v65.i4.a1.2016","article-title":"Review of Chromosome Races in Blind Mole Rats (Spalax and Nannospalax)","volume":"65","author":"Arslan","year":"2016","journal-title":"Folia Zool."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Savi\u0107, I., \u0106irovi\u0107, D., and Bugarski-Stanojevi\u0107, V. (2017). Exceptional Chromosomal Evolution and Cryptic Speciation of Blind Mole Rats Nannospalax Leucodon (Spalacinae, Rodentia) from South-Eastern Europe. Genes, 8.","DOI":"10.3390\/genes8110292"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1007\/s42991-020-00019-9","article-title":"16S rRNA Gene Polymorphism Supports Cryptic Speciation within the Lesser Blind Mole Rat Nannospalax Leucodon Superspecies (Rodentia: Spalacidae)","volume":"100","author":"Kataranovski","year":"2020","journal-title":"Mamm. Biol."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Bugarski-Stanojevi\u0107, V., Stamenkovi\u0107, G., Joji\u0107, V., \u0106osi\u0107, N., \u0106irovi\u0107, D., Stojkovi\u0107, O., Veli\u010dkovi\u0107, J., and Savi\u0107, I. (2022). Cryptic Diversity of the European Blind Mole Rat Nannospalax Leucodon Species Complex: Implications for Conservation. Animals, 12.","DOI":"10.3390\/ani12091097"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Bugarski-Stanojevi\u0107, V., \u0110oki\u0107, M., Stamenkovi\u0107, G., Bari\u0161i\u0107 Klisari\u0107, N., Stojkovi\u0107, O., Joji\u0107, V., and Savi\u0107, I. (2024). A Cryptic Subterranean Mammal Species, the Lesser Blind Mole Rat (Nannospalax leucodon syrmiensis)\u2014Retreated but Not Extinct. Animals, 14.","DOI":"10.3390\/ani14050774"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1007\/s13364-026-00872-y","article-title":"Are Five Cryptic Species of the European Lesser Blind Mole Rat, Nannospalax Leucodon (Rodentia: Spalacidae), Sexually Dimorphic Based on Cranial Variation?","volume":"71","author":"Kataranovski","year":"2026","journal-title":"Mammal. Res."},{"key":"ref_33","first-page":"121","article-title":"Preliminarni Spisak Vrsta Za Crvenu Listu Ki\u010dmenjaka Srbije","volume":"43","author":"Simonov","year":"1990","journal-title":"Za\u0161t. Prir."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1007\/s42977-024-00204-8","article-title":"Conservation Status of the Blind Mole Rat Populations in Hungary (Rodentia: Spalacinae: Nannospalax) Revisited","volume":"74","author":"Csorba","year":"2023","journal-title":"Biol. Futur."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1556\/1777.2026.00131","article-title":"A Chromosomal and Integrative Taxonomic Revision of Anatolian Blind Mole Rats (Nannospalax): Elevating Cytotypes to Species","volume":"72","author":"Matur","year":"2026","journal-title":"Anim. Taxon. Ecol."},{"key":"ref_36","first-page":"115","article-title":"Die Verbreitung der Karyotypen der Blindmaus Spalax (Mesospalax) in Jugoslawien","volume":"26","year":"1974","journal-title":"Arch. Biol. Nauka"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"19392","DOI":"10.1073\/pnas.1217211109","article-title":"Cancer Resistance in the Blind Mole Rat Is Mediated by Concerted Necrotic Cell Death Mechanism","volume":"109","author":"Gorbunova","year":"2012","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"89","DOI":"10.5735\/086.057.0110","article-title":"Multi-Locus Genetic Identification of a Newly Discovered Population Reveals a Deep Genetic Divergence in European Blind Mole Rats (Rodentia: Spalacidae: Nannospalax)","volume":"57","author":"Csorba","year":"2020","journal-title":"Ann. Zool. Fenn."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"14948","DOI":"10.1038\/s41598-020-71835-9","article-title":"Inter Simple Sequence Repeat Markers to Assess Genetic Diversity of the Desert Date (Balanites aegyptiaca Del.) for Sahelian Ecosystem Restoration","volume":"10","author":"Medraoui","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1515\/sg-2006-0017","article-title":"Identification of Species-Diagnostic ISSR Markers for Six Eucalyptus Species","volume":"55","author":"Balasaravanan","year":"2006","journal-title":"Silvae Genet."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"626","DOI":"10.1016\/j.bse.2006.02.003","article-title":"Identification of Endangered Mediterranean Cyprinodontiform Fish by Means of DNA Inter-Simple Sequence Repeats (ISSRs)","volume":"34","author":"Maltagliati","year":"2006","journal-title":"Biochem. Syst. Ecol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1016\/j.jcz.2012.11.004","article-title":"Identification of the Sibling Species Apodemus Sylvaticus and Apodemus Flavicollis (Rodentia, Muridae)\u2014Comparison of Molecular Methods","volume":"252","year":"2013","journal-title":"Zool. Anz."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1007\/s13364-023-00735-w","article-title":"Rapid and Reliable Method for Identification of Three Medium-Sized Horseshoe Bat Species in Europe","volume":"69","author":"Budinski","year":"2024","journal-title":"Mammal Res."},{"key":"ref_44","first-page":"30","article-title":"Inter-Simple Sequence Repeat (ISSR) Markers: Are We Doing It Right","volume":"9","author":"Ng","year":"2015","journal-title":"ASM Sci. J."},{"key":"ref_45","first-page":"269","article-title":"Fast Sex Identification in Wild Mammals Using PCR Amplification of the Sry Gene","volume":"52","author":"Bryja","year":"2003","journal-title":"Folia Zool."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1258\/00236770460734416","article-title":"Long-Term Anaesthesia Using Inhalatory Isoflurane in Different Strains of Mice\u2014The Haemodynamic Effects","volume":"38","author":"Veihelmann","year":"2004","journal-title":"Lab. Anim."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1007\/978-1-0716-3226-0_31","article-title":"Tools for Drawing Informative Idiograms","volume":"Volume 2672","author":"Heitkam","year":"2023","journal-title":"Plant Cytogenetics and Cytogenomics"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1080\/14772000.2011.560970","article-title":"Comparative Study of the Phylogenetic Structure in Six Apodemus Species (Mammalia, Rodentia) Inferred from ISSR-PCR Data","volume":"9","year":"2011","journal-title":"Syst. Biodivers."},{"key":"ref_49","unstructured":"Lazar, I., and Lazar Sr, I. (2022, December 01). GelAnalyzer 19.1. Available online: https:\/\/www.gelanalyzer.com."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1408","DOI":"10.2527\/1995.7351408x","article-title":"Sex Identification in Mammals with Polymerase Chain Reaction and Its Use to Examine Sex Effects on Diameter of Day-10 or-11 Pig Embryos","volume":"73","author":"Pomp","year":"1995","journal-title":"J. Anim. Sci."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"2772","DOI":"10.1093\/nar\/21.11.2772","article-title":"Comparative HMG-Box Sequences of the SRY Gene between Sheep, Cattle and Goats","volume":"21","author":"Payen","year":"1993","journal-title":"Nucleic Acids Res."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1007\/s003359900158","article-title":"High Sequence Identity between the SRY HMG Box from Humans and Insectivores","volume":"7","author":"Bullejos","year":"1996","journal-title":"Mamm. Genome"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1279","DOI":"10.1038\/nbt1290-1279","article-title":"Amplification of the Zfy and Zfx Genes for Sex Identification in Humans, Cattle, Sheep and Goats","volume":"8","author":"Aasen","year":"1990","journal-title":"Nat. Biotechnol."},{"key":"ref_54","first-page":"343","article-title":"Efficacy of Cryptic Rodents and Challenges for Conservation in Africa: A Review","volume":"8","author":"Ahmed","year":"2023","journal-title":"J. Wildl. Biodivers."},{"key":"ref_55","unstructured":"Savi\u0107, I., and Soldatovi\u0107, B. (1984). Karyotype Evolution and Taxonomy of the Genus Nannospalax Palmer, 1903, Mammalia, in Europe, Department of Natural and Mathematical, Serbian Academy of Sciences and Arts."},{"key":"ref_56","first-page":"5","article-title":"Karyotype Analysis and Cytogenetic Aspects of Speciation in the Genus Spalax","volume":"52","author":"Soldatovic","year":"1977","journal-title":"Zb. Prir. Nauk. Mat. Srp."},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Manov, I., Hirsh, M., Iancu, T.C., Malik, A., Sotnichenko, N., Band, M., Avivi, A., and Shams, I. (2013). Pronounced Cancer Resistance in a Subterranean Rodent, the Blind Mole-Rat, Spalax: In Vivo and in Vitroevidence. BMC Biol., 11.","DOI":"10.1186\/1741-7007-11-91"},{"key":"ref_58","unstructured":"Soldatovi\u0107, B. (1971). Cytogenetic Study of the Speciation of the Genus Spalax in Yugoslavia. 1971. [Ph.D. Thesis, University of Beograd]. (In Serbian)."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"zlaf186","DOI":"10.1093\/zoolinnean\/zlaf186","article-title":"Genome-Wide SNP and mtDNA Phylogeny of Blind Mole Rats (Spalacinae Gray, 1821) Reflects a Complex History of Relictualism, Expansion, and Speciation","volume":"206","author":"Yanchukov","year":"2026","journal-title":"Zool. J. Linn. Soc."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1098\/rspb.2002.2218","article-title":"Biological Identifications through DNA Barcodes","volume":"270","author":"Hebert","year":"2003","journal-title":"Proc. R. Soc. Lond. Ser. B Biol. Sci."},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Lu, L., Chesters, D., Zhang, W., Li, G., Ma, Y., Ma, H., Song, X., Wu, H., Meng, F., and Zhu, C. (2012). Small Mammal Investigation in Spotted Fever Focus with DNA-Barcoding and Taxonomic Implications on Rodents Species from Hainan of China. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0043479"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1111\/1755-0998.12279","article-title":"DNA Barcoding of Murinae (Rodentia: Muridae) and Arvicolinae (Rodentia: Cricetidae) Distributed in China","volume":"15","author":"Li","year":"2015","journal-title":"Mol. Ecol. Resour."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"15","DOI":"10.12933\/therya-18-430","article-title":"Rodents of the Eastern and Western Slopes of the Tropical Andes: Phylogenetic and Taxonomic Insights Using DNA Barcodes","volume":"9","author":"Pinto","year":"2018","journal-title":"Therya"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1080\/14772000.2016.1203371","article-title":"DNA Barcodes as a Tool in Biodiversity Research: Testing Pre-Existing Taxonomic Hypotheses in Delphic Apollo Butterflies (Lepidoptera, Papilionidae)","volume":"14","author":"Lukhtanov","year":"2016","journal-title":"Syst. Biodivers."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1023\/A:1020691618797","article-title":"Inter Simple Sequence Repeat (ISSR) Polymorphism and Its Application in Plant Breeding","volume":"128","author":"Sarla","year":"2002","journal-title":"Euphytica"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1006\/geno.1994.1151","article-title":"Genome Fingerprinting by Simple Sequence Repeat (SSR)-Anchored Polymerase Chain Reaction Amplification","volume":"20","author":"Zietkiewicz","year":"1994","journal-title":"Genomics"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"890","DOI":"10.1139\/g02-061","article-title":"Highly Informative Nature of Inter Simple Sequence Repeat (ISSR) Sequences Amplified Using Tri-and Tetra-Nucleotide Primers from DNA of Cauliflower (Brassica oleracea var. botrytis L.)","volume":"45","author":"Bornet","year":"2002","journal-title":"Genome"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1270\/jsbbs.20014","article-title":"ISSR Markers to Assess Genetic Diversity of Cultivated Populations from Artificial Selection of Stevia rebaudiana (Bert.) Bertoni","volume":"70","author":"Luz","year":"2020","journal-title":"Breed. Sci."},{"key":"ref_69","first-page":"31","article-title":"Monitoring Protocols for the Evaluation of the Impact of Wild Boar (Sus scrofa) Rooting on Plants and Animals in Forest Ecosystems","volume":"25","author":"Fagiani","year":"2014","journal-title":"Hystrix Ital. J. Mammal."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/j.indcrop.2018.04.062","article-title":"Genome Size and ISSR Markers for Mentha L. (Lamiaceae) Genetic Diversity Assessment and Species Identification","volume":"120","author":"Jedrzejczyk","year":"2018","journal-title":"Ind. Crops Prod."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"107958","DOI":"10.1016\/j.ympev.2023.107958","article-title":"Evolutionary History and Systematics of European Blind Mole Rats (Rodentia: Spalacidae: Nannospalax): Multilocus Phylogeny and Species Delimitation in a Puzzling Group","volume":"190","author":"Mizsei","year":"2024","journal-title":"Mol. Phylogenet. Evol."},{"key":"ref_72","unstructured":"Edition, F. (1999). International Code of Zoological Nomenclature, International Trust for Zoological Nomenclature."},{"key":"ref_73","unstructured":"IUCN Nannospalax Leucodon, and Rusin, M. (2024). The IUCN Red List of Threatened Species 2024, International Union for Conservation of Nature. E.T14328A221788646 2023."}],"container-title":["Life"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-1729\/16\/9\/1408\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T13:02:28Z","timestamp":1787662948000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-1729\/16\/9\/1408"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,25]]},"references-count":73,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["life16091408"],"URL":"https:\/\/doi.org\/10.3390\/life16091408","relation":{},"ISSN":["2075-1729"],"issn-type":[{"value":"2075-1729","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,25]]}}}