{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T17:56:42Z","timestamp":1792173402520,"version":"4.4.0"},"reference-count":50,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2017,2,8]],"date-time":"2017-02-08T00:00:00Z","timestamp":1486512000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Materials"],"abstract":"<jats:p>Diseases in articular cartilages have affected millions of people globally. Although the biochemical and cellular composition of articular cartilages is relatively simple, there is a limitation in the self-repair ability of the cartilage. Therefore, developing strategies for cartilage repair is very important. Here, we report on a new liquid resin preparation process of water-based polyurethane based photosensitive materials with hyaluronic acid with application of the materials for 3D printed customized cartilage scaffolds. The scaffold has high cytocompatibility and is one that closely mimics the mechanical properties of articular cartilages. It is suitable for culturing human Wharton\u2019s jelly mesenchymal stem cells (hWJMSCs) and the cells in this case showed an excellent chondrogenic differentiation capacity. We consider that the 3D printing hybrid scaffolds may have potential in customized tissue engineering and also facilitate the development of cartilage tissue engineering.<\/jats:p>","DOI":"10.3390\/ma10020136","type":"journal-article","created":{"date-parts":[[2017,2,10]],"date-time":"2017-02-10T05:27:09Z","timestamp":1486704429000},"page":"136","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":135,"title":["3D Printing of Cytocompatible Water-Based Light-Cured Polyurethane with Hyaluronic Acid for Cartilage Tissue Engineering Applications"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0650-4344","authenticated-orcid":false,"given":"Ming-You","family":"Shie","sequence":"first","affiliation":[{"name":"3D Printing Medical Research Center, China Medical University Hospital, China Medical University, Taichung 40447, Taiwan"},{"name":"School of Dentistry, China Medical University, Taichung 40447, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen-Ching","family":"Chang","sequence":"additional","affiliation":[{"name":"3D Printing Medical Research Center, China Medical University Hospital, China Medical University, Taichung 40447, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-Ju","family":"Wei","sequence":"additional","affiliation":[{"name":"3D Printing Medical Research Center, China Medical University Hospital, China Medical University, Taichung 40447, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Hsin","family":"Huang","sequence":"additional","affiliation":[{"name":"3D Printing Medical Research Center, China Medical University Hospital, China Medical University, Taichung 40447, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chien-Han","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Medicine, College of Medicine, China Medical University, Taichung 40447, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng-Ting","family":"Shih","sequence":"additional","affiliation":[{"name":"3D Printing Medical Research Center, China Medical University Hospital, China Medical University, Taichung 40447, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Wen","family":"Chen","sequence":"additional","affiliation":[{"name":"3D Printing Medical Research Center, China Medical University Hospital, China Medical University, Taichung 40447, Taiwan"},{"name":"Graduate Institute of Biomedical Sciences, China Medical University, Taichung 40447, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Fang","family":"Shen","sequence":"additional","affiliation":[{"name":"3D Printing Medical Research Center, China Medical University Hospital, China Medical University, Taichung 40447, Taiwan"},{"name":"Department of Bioinformatics and Medical Engineering, Asia University, Taichung 40447, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2017,2,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1007\/s00441-004-1025-0","article-title":"In vivo chondrogenesis of adult bone-marrow-derived autologous mesenchymal stem cells","volume":"319","author":"Chen","year":"2005","journal-title":"Cell Tissue Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1177\/03635465980260022701","article-title":"Articular cartilage repair","volume":"26","author":"Newman","year":"1998","journal-title":"Am. J. Sports Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1016\/j.actbio.2008.09.017","article-title":"Bilayered chitosan-based scaffolds for osteochondral tissue engineering: Influence of hydroxyapatite on in vitro cytotoxicity and dynamic bioactivity studies in a specific double-chamber bioreactor","volume":"5","author":"Malafaya","year":"2009","journal-title":"Acta Biomater."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2526","DOI":"10.1002\/jbm.a.34147","article-title":"Functional biomaterials for cartilage regeneration","volume":"100","author":"Ge","year":"2012","journal-title":"J. Biomed. Mater. Res. A"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1002\/jbm.b.33011","article-title":"Collagen hydrogel as an immunomodulatory scaffold in cartilage tissue engineering","volume":"102","author":"Yuan","year":"2014","journal-title":"J. Biomed. Mater. Res. B Appl. Biomater."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"972","DOI":"10.1002\/adfm.201102884","article-title":"Creating a living hyaline cartilage graft free from non-cartilaginous constituents: An intermediate role of a biomaterial scaffold","volume":"22","author":"Su","year":"2012","journal-title":"Adv. Funct. Mater."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"25005","DOI":"10.1088\/1748-6041\/8\/2\/025005","article-title":"Poly(l-lactide-co-caprolactone) scaffolds enhanced with poly(beta-hydroxybutyrate-co-beta-hydroxyvalerate) microspheres for cartilage regeneration","volume":"8","author":"Li","year":"2013","journal-title":"Biomed. Mater."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1444","DOI":"10.1111\/j.1742-1241.2010.02420.x","article-title":"Current strategies for knee cartilage repair","volume":"64","author":"Kalson","year":"2010","journal-title":"Int. J. Clin. Pract."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"15001","DOI":"10.1088\/1758-5082\/5\/1\/015001","article-title":"Hybrid printing of mechanically and biologically improved constructs for cartilage tissue engineering applications","volume":"5","author":"Xu","year":"2013","journal-title":"Biofabrication"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1578","DOI":"10.1002\/adhm.201400018","article-title":"Synthesis and 3D printing of biodegradable polyurethane elastomer by a water-based process for cartilage tissue engineering applications","volume":"3","author":"Hung","year":"2014","journal-title":"Adv. Healthc. Mater."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1489","DOI":"10.1021\/acs.biomac.5b00188","article-title":"3D Bioprinting human chondrocytes with nanocellulose-alginate bioink for cartilage tissue engineering applications","volume":"16","author":"Markstedt","year":"2015","journal-title":"Biomacromolecules"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"6706","DOI":"10.1016\/j.biomaterials.2013.05.038","article-title":"Regeneration of a goat femoral head using a tissue-specific, biphasic scaffold fabricated with CAD\/CAM technology","volume":"34","author":"Ding","year":"2013","journal-title":"Biomaterials"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1089\/ten.tec.2012.0383","article-title":"Three-dimensional printing of soy protein scaffolds for tissue regeneration","volume":"19","author":"Chien","year":"2013","journal-title":"Tissue Eng. Part C Methods"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2028","DOI":"10.1016\/j.actbio.2009.12.033","article-title":"Porous polycaprolactone scaffold for cardiac tissue engineering fabricated by selective laser sintering","volume":"6","author":"Yeong","year":"2010","journal-title":"Acta Biomater."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Tsai, K.-Y., Lin, H.-Y., Chen, Y.-W., Lin, C.-Y., Hsu, T.-T., and Kao, C.-T. (2017). Laser Sintered Magnesium-Calcium Silicate\/Poly-\u03b5-Caprolactone Scaffold for Bone Tissue Engineering. Materials, 10.","DOI":"10.3390\/ma10010065"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"4739","DOI":"10.1016\/S0142-9612(02)00223-5","article-title":"A three-dimensional osteochondral composite scaffold for articular cartilage repair","volume":"23","author":"Sherwood","year":"2002","journal-title":"Biomaterials"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"3824","DOI":"10.1016\/j.biomaterials.2012.01.048","article-title":"Microfabrication of complex porous tissue engineering scaffolds using 3D projection stereolithography","volume":"33","author":"Gauvin","year":"2012","journal-title":"Biomaterials"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1080\/17452759.2012.673152","article-title":"Continuous digital light processing (cDLP): Highly accurate additive manufacturing of tissue engineered bone scaffolds","volume":"7","author":"Dean","year":"2012","journal-title":"Virtual Phys. Prototyp."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"5494","DOI":"10.1016\/j.jmatprotec.2009.05.004","article-title":"Fabrication of 3D biocompatible\/biodegradable micro-scaffolds using dynamic mask projection microstereolithography","volume":"209","author":"Choi","year":"2009","journal-title":"J. Mater. Process. Technol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1186\/s13036-015-0001-4","article-title":"Recent advances in 3D printing of biomaterials","volume":"9","author":"Chia","year":"2015","journal-title":"J. Biol. Eng."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"3850","DOI":"10.1016\/j.actbio.2011.06.039","article-title":"Preparation of poly(\u03b5-caprolactone)-based tissue engineering scaffolds by stereolithography","volume":"7","author":"Elomaa","year":"2011","journal-title":"Acta Biomater."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"3801","DOI":"10.1016\/j.biomaterials.2009.03.055","article-title":"A poly(d,l-lactide) resin for the preparation of tissue engineering scaffolds by stereolithography","volume":"30","author":"Melchels","year":"2009","journal-title":"Biomaterials"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.jconrel.2010.07.111","article-title":"Designed biodegradable hydrogel structures prepared by stereolithography using poly(ethylene glycol)\/poly(d,l-lactide)-based resins","volume":"148","author":"Seck","year":"2010","journal-title":"J. Control. Release"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"80","DOI":"10.22203\/eCM.v016a09","article-title":"Degradable polymeric materials for osteosynthesis: Tutorial","volume":"16","author":"Eglin","year":"2008","journal-title":"Eur. Cell. Mater."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"762","DOI":"10.1016\/j.progpolymsci.2007.05.017","article-title":"Biodegradable polymers as biomaterials","volume":"32","author":"Nair","year":"2007","journal-title":"Prog. Polym. Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1","DOI":"10.22203\/eCM.v005a01","article-title":"Biodegradable synthetic polymers for tissue engineering","volume":"5","author":"Gunatillake","year":"2003","journal-title":"Eur. Cell. Mater."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1517\/17425247.5.5.543","article-title":"Critical factors in the design of growth factor releasing scaffolds for cartilage tissue engineering","volume":"5","author":"Sohier","year":"2008","journal-title":"Expert Opin. Drug Deliv."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Szycher, M. (1999). Scycher\u2019s Handbook of Polyurethanes, CRC Press.","DOI":"10.1201\/9781482273984"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2033","DOI":"10.1002\/pola.24632","article-title":"Synthesis and micellization of new biodegradable phosphorylcholine-capped polyurethane","volume":"49","author":"Wang","year":"2011","journal-title":"J. Polym. Sci. Part A Polym. Chem."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1002\/masy.200451308","article-title":"Water soluble, photocurable resins for rapid prototyping applications","volume":"217","author":"Stampfl","year":"2004","journal-title":"Macromol. Symp."},{"key":"ref_31","unstructured":"Claycomb, J.R., and Tran, J.Q.P. (2010). Introductory Biophysics: Perspectives on the Living State, Jones and Bartlett Learning."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1002\/mabi.201200471","article-title":"Gelatin-methacrylamide hydrogels as potential biomaterials for fabrication of tissue-engineered cartilage constructs","volume":"13","author":"Schuurman","year":"2013","journal-title":"Macromol. Biosci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"139","DOI":"10.5405\/jmbe.1109","article-title":"Enhanced migration of Wharton\u2019s Jelly mesenchymal stem cells grown on polyurethane nanocomposites","volume":"33","author":"Huang","year":"2013","journal-title":"J. Med. Biol. Eng."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"180","DOI":"10.7326\/0003-4819-157-3-201208070-00473","article-title":"Viscosupplementation for osteoarthritis of the knee: A systematic review and meta-analysis","volume":"157","author":"Rutjes","year":"2012","journal-title":"Ann. Intern. Med."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1016\/j.arthro.2012.05.891","article-title":"Scaffold-based repair for cartilage healing: A systematic review and technical note","volume":"29","author":"Filardo","year":"2013","journal-title":"Arthroscopy"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1016\/0012-1606(86)90214-9","article-title":"Hyaluronic acid bonded to cell-culture surfaces stimulates chondrogenesis in stage 24 limb mesenchyme cell cultures","volume":"114","author":"Kujawa","year":"1986","journal-title":"Dev. Biol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"8771","DOI":"10.1016\/j.biomaterials.2011.08.073","article-title":"Hydrogel design for cartilage tissue engineering: A case study with hyaluronic acid","volume":"32","author":"Kim","year":"2011","journal-title":"Biomaterials"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"2846","DOI":"10.1177\/0363546516656179","article-title":"One-stage cartilage repair using a hyaluronic acid\u2013based scaffold with activated bone marrow-derived mesenchymal stem cells compared with microfracture: Five-year follow-up","volume":"44","author":"Gobbi","year":"2016","journal-title":"Am. J. Sport. Med."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1016\/0304-4165(78)90163-0","article-title":"Raman spectroscopy: A structural probe of glycosaminoglycans","volume":"541","author":"Bansil","year":"1978","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"790","DOI":"10.1002\/term.378","article-title":"Controlled Gelation and Degradation Rates of Injectable Hyaluronic Acid-based Hydrogels through a Double Crosslinking Strategy","volume":"5","author":"Tan","year":"2011","journal-title":"J. Tissue Eng. Regen. Med."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"2035","DOI":"10.1016\/j.actbio.2011.02.011","article-title":"In vitro and in vivo degradation profile of aliphatic polyesters subjected to electron beam sterilization","volume":"7","author":"Schander","year":"2011","journal-title":"Acta Biomater."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1016\/j.msec.2015.05.043","article-title":"Hydrophilic polyurethane matrix promotes chondrogenesis of mesenchymal stem cells","volume":"54","author":"Nalluri","year":"2015","journal-title":"Mater. Sci. Eng. C Mater. Biol. Appl."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1925","DOI":"10.1016\/j.biomaterials.2004.06.021","article-title":"Hyaluronic acid modified biodegradable scaffolds for cartilage tissue engineering","volume":"26","author":"Yoo","year":"2005","journal-title":"Biomaterials"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"4573","DOI":"10.1016\/j.biomaterials.2006.04.013","article-title":"Cartilage regeneration using mesenchymal stem cells and a PLGA-gelatin\/chondroitin\/hyaluronate hybrid scaffold","volume":"27","author":"Fan","year":"2006","journal-title":"Biomaterials"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Choong, Y.Y.C., Maleksaeedi, S., Eng, H., Su, P.-C., and Wei, J. (2016). Curing characteristics of shape memory polymers in 3D projection and laser stereolithography. Virtual Phys. Prototyp., 1\u20138.","DOI":"10.1080\/17452759.2016.1254845"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1080\/17452759.2016.1244085","article-title":"4D printing shape memory polymers for dynamic jewellery and fashionwear","volume":"11","author":"Zarek","year":"2016","journal-title":"Virtual Phys. Prototyp."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1080\/17452759.2016.1198630","article-title":"Current status of 4D printing technology and the potential of light-reactive smart materials as 4D printable materials","volume":"11","author":"Leist","year":"2016","journal-title":"Virtual Phys. Prototyp."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1358","DOI":"10.1366\/000370206779321454","article-title":"Raman spectra of biological samples: A study of preprocessing methods","volume":"60","author":"Afseth","year":"2006","journal-title":"Appl. Spectrosc."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"8363","DOI":"10.1016\/j.biomaterials.2012.08.023","article-title":"In vivo biocompatibility and osteogenesis of electrospun poly(epsilon-caprolactone)-poly(ethylene glycol)-poly(epsilon-caprolactone)\/nano-hydroxyapatite composite scaffold","volume":"33","author":"Fu","year":"2012","journal-title":"Biomaterials"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/0012-1606(77)90110-5","article-title":"Stage-related capacity for limb chondrogenesis in cell culture","volume":"60","author":"Ahrens","year":"1977","journal-title":"Dev. Biol."}],"container-title":["Materials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1996-1944\/10\/2\/136\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T18:27:41Z","timestamp":1760207261000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1996-1944\/10\/2\/136"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,2,8]]},"references-count":50,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2017,2]]}},"alternative-id":["ma10020136"],"URL":"https:\/\/doi.org\/10.3390\/ma10020136","relation":{"has-preprint":[{"id-type":"doi","id":"10.20944\/preprints201611.0150.v1","asserted-by":"object"}]},"ISSN":["1996-1944"],"issn-type":[{"value":"1996-1944","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,2,8]]}}}