{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T04:38:00Z","timestamp":1790483880285,"version":"4.1.0"},"reference-count":116,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2019,7,18]],"date-time":"2019-07-18T00:00:00Z","timestamp":1563408000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Materials"],"abstract":"<jats:p>Biosensors are analytical devices combining a physical sensor with a part of biological origin providing sensitivity and selectivity toward analyte. Biological warfare agents are infectious microorganisms or toxins with the capability to harm or kill humans. They can be produced and spread by a military or misused by a terrorist group. For example, Bacillus anthracis, Francisella tularensis, Brucella sp., Yersinia pestis, staphylococcal enterotoxin B, botulinum toxin and orthopoxviruses are typical biological warfare agents. Biosensors for biological warfare agents serve as simple but reliable analytical tools for the both field and laboratory assay. There are examples of commercially available biosensors, but research and development of new types continue and their application in praxis can be expected in the future. This review summarizes the facts and role of biosensors in the biological warfare agents\u2019 assay, and shows current commercially available devices and trends in research of the news. Survey of actual literature is provided.<\/jats:p>","DOI":"10.3390\/ma12142303","type":"journal-article","created":{"date-parts":[[2019,7,19]],"date-time":"2019-07-19T03:14:41Z","timestamp":1563506081000},"page":"2303","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":68,"title":["Current Trends in the Biosensors for Biological Warfare Agents Assay"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8804-8356","authenticated-orcid":false,"given":"Miroslav","family":"Pohanka","sequence":"first","affiliation":[{"name":"Faculty of Military Health Sciences, University of Defense, Trebesska 1575, CZ-50001 Hradec Kralove, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,7,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Janik, E., Ceremuga, M., Saluk-Bijak, J., and Bijak, M. (2019). Biological toxins as the potential tools for bioterrorism. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20051181"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1177\/0897190012442351","article-title":"Bioterrorism: Toxins as weapons","volume":"25","author":"Anderson","year":"2012","journal-title":"J. Pharm. Pract."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"403","DOI":"10.20506\/rst.36.2.2661","article-title":"Introduction\u2014Biological threat reduction","volume":"36","author":"Beckham","year":"2017","journal-title":"Rev. Sci. Tech."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1080\/09674845.2005.11732685","article-title":"Bacteria as potential tools in bioterrorism, with an emphasis on bacterial toxins","volume":"62","author":"Clarke","year":"2005","journal-title":"Br. J. Biomed. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"428","DOI":"10.15690\/vramn.v70.i4.1408","article-title":"Development of specific therapy to category a toxic infections","volume":"4","author":"Kolesnikov","year":"2015","journal-title":"Vestn. Ross Akad. Med. Nauk"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1002\/mas.21509","article-title":"Direct analysis in real time-mass spectrometry (dart-ms) in forensic and security applications","volume":"37","author":"Pavlovich","year":"2018","journal-title":"Mass Spectrom. Rev."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/j.biotechadv.2014.11.012","article-title":"Ricin detection: Tracking active toxin","volume":"33","author":"Bozza","year":"2015","journal-title":"Biotechnol. Adv."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1002\/jms.3747","article-title":"Mass spectrometry for the detection of bioterrorism agents: From environmental to clinical applications","volume":"51","author":"Duriez","year":"2016","journal-title":"J. Mass Spectrom."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"43","DOI":"10.3109\/1040841X.2012.691457","article-title":"Botulinum neurotoxin: Where are we with detection technologies?","volume":"39","author":"Singh","year":"2013","journal-title":"Crit. Rev. Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1021\/ac069343r","article-title":"Ms on the bioterror front lines","volume":"78","author":"Cottingham","year":"2006","journal-title":"Anal. Chem."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1016\/j.taap.2005.02.040","article-title":"Mass spectrometry in identification of ecotoxicants including chemical and biological warfare agents","volume":"207","author":"Lebedev","year":"2005","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.chroma.2006.08.078","article-title":"Trends in detection of warfare agents. Detection methods for ricin, staphylococcal enterotoxin b and t-2 toxin","volume":"1133","author":"Ler","year":"2006","journal-title":"J. Chromatogr. A"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0021-9673(98)00338-0","article-title":"Chromatography and mass spectrometry of chemical warfare agents, toxins and related compounds: State of the art and future prospects","volume":"814","author":"Kientz","year":"1998","journal-title":"J. Chromatogr. A"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1255\/ejms.583","article-title":"Mass spectrometric analysis of chemical warfare agents and their degradation products in soil and synthetic samples","volume":"9","author":"Hancock","year":"2003","journal-title":"Eur. J. Mass Spectrom."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"6557","DOI":"10.1128\/AEM.00779-07","article-title":"Development of quantitative real-time pcr assays for detection and quantification of surrogate biological warfare agents in building debris and leachate","volume":"73","author":"Saikaly","year":"2007","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1322","DOI":"10.1128\/AEM.03478-13","article-title":"Cross-institute evaluations of inhibitor-resistant pcr reagents for direct testing of aerosol and blood samples containing biological warfare agent DNA","volume":"80","author":"Minogue","year":"2014","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"458","DOI":"10.14429\/dsj.66.10698","article-title":"Development of a polymerase chain reaction assay for detection of burkholderia mallei, a potent biological warfare agent","volume":"66","author":"Pal","year":"2016","journal-title":"Def. Sci. J."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"4","DOI":"10.2478\/s11696-014-0542-x","article-title":"Biosensors containing acetylcholinesterase and butyrylcholinesterase as recognition tools for detection of various compounds","volume":"69","author":"Pohanka","year":"2015","journal-title":"Chem. Pap."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Pohanka, M. (2018). Overview of piezoelectric biosensors, immunosensors and DNA sensors and their applications. Materials, 11.","DOI":"10.3390\/ma11030448"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"496","DOI":"10.20964\/2017.01.44","article-title":"The piezoelectric biosensors: Principles and applications, a review","volume":"12","author":"Pohanka","year":"2017","journal-title":"Int. J. Electrochem. Sci."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Bochenkov, V.E., and Shabatina, T.I. (2018). Chiral plasmonic biosensors. Biosensors, 8.","DOI":"10.3390\/bios8040120"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1007\/s00604-019-3420-y","article-title":"Dengue virus: A review on advances in detection and trends\u2014From conventional methods to novel biosensors","volume":"186","author":"Mahmoudi","year":"2019","journal-title":"Mikrochim. Acta"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Nguyen, H.H., Lee, S.H., Lee, U.J., Fermin, C.D., and Kim, M. (2019). Immobilized enzymes in biosensor applications. Materials, 12.","DOI":"10.3390\/ma12010121"},{"key":"ref_24","first-page":"185","article-title":"Biosensor technology for detecting biological warfare agents: Recent progress and future trends","volume":"57","author":"Gooding","year":"2006","journal-title":"Anal. Chim. Acta"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"185","DOI":"10.14429\/dsj.57.1760","article-title":"Biosensors for biological warfare agent detection","volume":"57","author":"Pohanka","year":"2007","journal-title":"Def. Sci. J."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"294","DOI":"10.3184\/003685013X13777066241280","article-title":"Development of biosensors for the detection of biological warfare agents: Its issues and challenges","volume":"96","author":"Kumar","year":"2013","journal-title":"Sci. Prog."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1996","DOI":"10.1039\/C7CS00573C","article-title":"Optical nano-biosensing interface via nucleic acid amplification strategy: Construction and application","volume":"47","author":"Zhou","year":"2018","journal-title":"Chem. Soc. Rev."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Li, Z., Zhang, W., and Xing, F. (2019). Graphene optical biosensors. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20102461"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Mowbray, S.E., and Amiri, A.M. (2019). A brief overview of medical fiber optic biosensors and techniques in the modification for enhanced sensing ability. Diagnostics, 9.","DOI":"10.3390\/diagnostics9010023"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"25208","DOI":"10.3390\/s151025208","article-title":"Fiber-optic chemical sensors and fiber-optic bio-sensors","volume":"15","author":"Pospisilova","year":"2015","journal-title":"Sensors"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.aca.2016.08.049","article-title":"Fluorescence based fiber optic and planar waveguide biosensors. A review","volume":"943","author":"Valdes","year":"2016","journal-title":"Anal. Chim. Acta"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1080\/10408347.2015.1045119","article-title":"Fiber optic surface plasmon resonance-based biosensor technique: Fabrication, advancement, and application","volume":"46","author":"Liang","year":"2016","journal-title":"Crit. Rev. Anal. Chem."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1038\/s41587-019-0045-y","article-title":"Wearable biosensors for healthcare monitoring","volume":"37","author":"Kim","year":"2019","journal-title":"Nat. Biotechnol."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Steglich, P., Hulsemann, M., Dietzel, B., and Mai, A. (2019). Optical biosensors based on silicon-on-insulator ring resonators: A review. Molecules, 24.","DOI":"10.20944\/preprints201901.0012.v1"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1805043","DOI":"10.1002\/adma.201805043","article-title":"Graphene oxide as an optical biosensing platform: A progress report","volume":"31","author":"Merkoci","year":"2019","journal-title":"Adv. Mater."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Nawrot, W., Drzozga, K., Baluta, S., Cabaj, J., and Malecha, K. (2018). A fluorescent biosensors for detection vital body fluids\u2019 agents. Sensors, 18.","DOI":"10.3390\/s18082357"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"15458","DOI":"10.3390\/s140815458","article-title":"Recent developments in optical detection technologies in lab-on-a-chip devices for biosensing applications","volume":"14","author":"Pires","year":"2014","journal-title":"Sensors"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1209","DOI":"10.1016\/j.bbagen.2018.02.008","article-title":"Optical fiber tips for biological applications: From light confinement, biosensing to bioparticles manipulation","volume":"1862","author":"Paiva","year":"2018","journal-title":"Biochim. Biophys. Acta Gen. Subj."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1016\/j.bios.2004.01.029","article-title":"A rapid detection method for vaccinia virus, the surrogate for smallpox virus","volume":"20","author":"Donaldson","year":"2004","journal-title":"Biosen. Bioelectron."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1093\/jat\/23.6.460","article-title":"A rapid reusable fiber optic biosensor for detecting cocaine metabolites in urine","volume":"23","author":"Nath","year":"1999","journal-title":"J. Anal. Toxicol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"937","DOI":"10.1016\/S0956-5663(97)00027-4","article-title":"Fiber optic-based biosensor for ricin","volume":"12","author":"Narang","year":"1997","journal-title":"Biosens. Bioelectron."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1128\/jcm.33.2.336-341.1995","article-title":"Detection of yersinia pestis fraction 1 antigen with a fiber optic biosensor","volume":"33","author":"Cao","year":"1995","journal-title":"J. Clin. Microbiol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"711","DOI":"10.4315\/0362-028X-62.7.711","article-title":"Rapid detection of escherichia coli o157:H7 in ground beef using a fiber-optic biosensor","volume":"62","author":"DeMarco","year":"1999","journal-title":"J. Food Prot."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1006\/abio.1996.0006","article-title":"Quantitating staphylococcal enterotoxin b in diverse media using a portable fiber-optic biosensor","volume":"233","author":"Tempelman","year":"1996","journal-title":"Anal. Biochem."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1016\/S0956-5663(99)00053-6","article-title":"Multi-analyte interrogation using the fiber optic biosensor","volume":"14","author":"Anderson","year":"2000","journal-title":"Biosens. Bioelectron."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"650","DOI":"10.2174\/1389557517666170120153342","article-title":"Quantum dots in the therapy: Current trends and perspectives","volume":"17","author":"Pohanka","year":"2017","journal-title":"Mini Rev. Med. Chem."},{"key":"ref_47","first-page":"1","article-title":"Nanobiosensors and fluorescence based biosensors: An overview","volume":"10","author":"Girigoswami","year":"2019","journal-title":"Int. J. Nano Dimens."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1016\/j.talanta.2019.02.098","article-title":"One-step synthesis of boron-doped graphene quantum dots for fluorescent sensors and biosensor","volume":"199","author":"Ge","year":"2019","journal-title":"Talanta"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1629","DOI":"10.1016\/j.talanta.2010.08.033","article-title":"Screen-printed biosensors in microbiology; A review","volume":"82","year":"2010","journal-title":"Talanta"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"610","DOI":"10.1016\/j.snb.2018.06.084","article-title":"Digital camera-based lipase biosensor for the determination of paraoxon","volume":"273","author":"Pohanka","year":"2018","journal-title":"Sens. Actuator B Chem."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1553","DOI":"10.1007\/s11696-017-0166-z","article-title":"Small camera as a handheld colorimetric tool in the analytical chemistry","volume":"71","author":"Pohanka","year":"2017","journal-title":"Chem. Pap."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"13752","DOI":"10.3390\/s150613752","article-title":"Photography by cameras integrated in smartphones as a tool for analytical chemistry represented by an butyrylcholinesterase activity assay","volume":"15","author":"Pohanka","year":"2015","journal-title":"Sensors"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"5531","DOI":"10.1021\/acsomega.8b00625","article-title":"Single-image-referenced colorimetric water quality detection using a smartphone","volume":"3","author":"Kilic","year":"2018","journal-title":"ACS Omega"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"2729","DOI":"10.1007\/s11696-018-0528-1","article-title":"Evaluation of tyrosinase inhibitory activity in salak (Salacca zalacca) extracts using the digital image-based colorimetric method","volume":"72","author":"Moonrungsee","year":"2018","journal-title":"Chem. Pap."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1076","DOI":"10.1134\/S1061934818110060","article-title":"Colorimetry in chemical analysis","volume":"73","author":"Monogarova","year":"2018","journal-title":"J. Anal. Chem."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1071\/EN18072","article-title":"Mobile-phone-based colourimetric analysis for determining nitrite content in water","volume":"15","author":"Puangpila","year":"2018","journal-title":"Environ. Chem."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"498","DOI":"10.1016\/j.snb.2018.03.094","article-title":"A ratiometric fluorescence visual test paper for an anthrax biomarker based on functionalized manganese-doped carbon dots","volume":"265","author":"Rong","year":"2018","journal-title":"Sens. Actuator B Chem."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"127006","DOI":"10.1117\/1.3662460","article-title":"Detection of anthrax lef with DNA-based photonic crystal sensors","volume":"16","author":"Zhang","year":"2011","journal-title":"J. Biomed. Opt."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"3004","DOI":"10.3390\/s110303004","article-title":"Photonic biosensor assays to detect and distinguish subspecies of francisella tularensis","volume":"11","author":"Cooper","year":"2011","journal-title":"Sensors"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.ab.2016.04.018","article-title":"A biolayer interferometry-based assay for rapid and highly sensitive detection of biowarfare agents","volume":"506","author":"Mechaly","year":"2016","journal-title":"Anal. Biochem."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.bios.2011.08.031","article-title":"Optical microchip array biosensor for multiplexed detection of bio-hazardous agents","volume":"30","author":"Bhatta","year":"2011","journal-title":"Biosens. Bioelectron."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"17953","DOI":"10.1038\/srep17953","article-title":"An optical biosensor assay for rapid dual detection of botulinum neurotoxins a and e","volume":"5","author":"Leveque","year":"2015","journal-title":"Sci. Rep."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1016\/j.bios.2014.10.050","article-title":"A graphene oxide based fluorescence resonance energy transfer (fret) biosensor for ultrasensitive detection of botulinum neurotoxin a (bont\/a) enzymatic activity","volume":"65","author":"Shi","year":"2015","journal-title":"Biosens. Bioelectron."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1039\/c0lc00431f","article-title":"Lensless ccd-based fluorometer using a micromachined optical soller collimator","volume":"11","author":"Balsam","year":"2011","journal-title":"Lab Chip"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.bios.2019.03.055","article-title":"A review of microfabricated electrochemical biosensors for DNA detection","volume":"134","author":"Blair","year":"2019","journal-title":"Biosen. Bioelectron."},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Lee, J.H., Park, S.J., and Choi, J.W. (2019). Electrical property of graphene and its application to electrochemical biosensing. Nanomaterials, 9.","DOI":"10.3390\/nano9020297"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"4303","DOI":"10.1007\/s00216-019-01640-5","article-title":"Novel paper-based electroanalytical tools for food surveillance","volume":"411","author":"Cinti","year":"2019","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Pohanka, M. (2019). Biosensors and bioassays based on lipases, principles and applications, a review. Molecules, 24.","DOI":"10.3390\/molecules24030616"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.cis.2018.11.001","article-title":"A review on electrochemical biosensing platform based on layered double hydroxides for small molecule biomarkers determination","volume":"262","author":"Asif","year":"2018","journal-title":"Adv. Colloid Interface Sci."},{"key":"ref_70","doi-asserted-by":"crossref","unstructured":"Zhou, Y., Fang, Y., and Ramasamy, R.P. (2019). Non-covalent functionalization of carbon nanotubes for electrochemical biosensor development. Sensors, 19.","DOI":"10.3390\/s19020392"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1002\/elan.200390019","article-title":"Electrochemical biosensors for detection of biological warfare agents","volume":"15","author":"Shah","year":"2003","journal-title":"Electroanalysis"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"927","DOI":"10.1016\/j.snb.2015.09.157","article-title":"Screen-printed electrode produced by printed-circuit board technology. Application to cancer biomarker detection by means of plastic antibody as sensing material","volume":"223","author":"Moreira","year":"2016","journal-title":"Sens. Actuator B Chem."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.electacta.2012.06.033","article-title":"A review of experimental aspects of electrochemical immunosensors","volume":"84","author":"Ricci","year":"2012","journal-title":"Electrochim. Acta"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"57","DOI":"10.32725\/jab.2008.008","article-title":"Electrochemical biosensors\u2014Principles and applications","volume":"6","author":"Pohanka","year":"2008","journal-title":"J. Appl. Biomed."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"2865","DOI":"10.1080\/00032719.2016.1166370","article-title":"Three-dimensional printing in analytical chemistry: Principles and applications","volume":"49","author":"Pohanka","year":"2016","journal-title":"Anal. Lett."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"15","DOI":"10.3390\/bios2010015","article-title":"Electrochemical biosensor for rapid and sensitive detection of magnetically extracted bacterial pathogens","volume":"2","author":"Settrington","year":"2012","journal-title":"Biosensors"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"640","DOI":"10.1016\/j.bios.2018.11.017","article-title":"A label-free impedimetric aptasensor for the detection of bacillus anthracis spore simulant","volume":"126","author":"Mazzaracchio","year":"2019","journal-title":"Biosens. Bioelectron."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1016\/S1452-3981(23)15882-2","article-title":"Selective and sensitive electrochemical DNA biosensor for the detection of bacillus anthracis","volume":"11","author":"Raveendran","year":"2016","journal-title":"Int. J. Electrochem. Sci."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"B187","DOI":"10.1149\/2.0551805jes","article-title":"Electrochemical detection of bacillus anthracis protective antigen gene using DNA biosensor based on stem-loop probe","volume":"165","author":"Ziolkowski","year":"2018","journal-title":"J. Electrochem. Soc."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"214003","DOI":"10.1088\/1361-6528\/ab0326","article-title":"Biosensing using arrays of vertical semiconductor nanowires: Mechanosensing and biomarker detection","volume":"30","author":"Lard","year":"2019","journal-title":"Nanotechnology"},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1016\/j.talanta.2018.12.042","article-title":"A review on nanostructured carbon quantum dots and their applications in biotechnology, sensors, and chemiluminescence","volume":"196","author":"Molaei","year":"2019","journal-title":"Talanta"},{"key":"ref_82","doi-asserted-by":"crossref","unstructured":"Luan, E., Shoman, H., Ratner, D.M., Cheung, K.C., and Chrostowski, L. (2018). Silicon photonic biosensors using label-free detection. Sensors, 18.","DOI":"10.20944\/preprints201809.0150.v2"},{"key":"ref_83","doi-asserted-by":"crossref","unstructured":"Piro, B., Mattana, G., and Reisberg, S. (2018). Transistors for chemical monitoring of living cells. Biosensors, 8.","DOI":"10.3390\/bios8030065"},{"key":"ref_84","doi-asserted-by":"crossref","unstructured":"Tran, D.P., Pham, T.T.T., Wolfrum, B., Offenhausser, A., and Thierry, B. (2018). Cmos-compatible silicon nanowire field-effect transistor biosensor: Technology development toward commercialization. Materials, 11.","DOI":"10.3390\/ma11050785"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"14010","DOI":"10.1002\/chem.201801358","article-title":"Current advances in semiconductor nanomaterial-based photoelectrochemical biosensing","volume":"24","author":"Zang","year":"2018","journal-title":"Chemistry"},{"key":"ref_86","first-page":"101","article-title":"Evaluation of two types of biosensors for immunoassay of botulinum toxin","volume":"31","author":"Choi","year":"1998","journal-title":"J. Biochem. Mol. Biol."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"3707","DOI":"10.1039\/C5LC00731C","article-title":"Paper diagnostic device for quantitative electrochemical detection of ricin at picomolar levels","volume":"15","author":"Cunningha","year":"2015","journal-title":"Lab Chip"},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/0956-5663(90)80023-7","article-title":"Piezoelectric crystal biosensors","volume":"5","author":"Suleiman","year":"1990","journal-title":"Biosens. Bioelectron."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"4632","DOI":"10.1002\/adma.201104365","article-title":"Progress in piezotronics and piezo-phototronics","volume":"24","author":"Wang","year":"2012","journal-title":"Adv. Mater."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"1802084","DOI":"10.1002\/adma.201802084","article-title":"Piezoelectric biomaterials for sensors and actuators","volume":"31","author":"Chorsi","year":"2019","journal-title":"Adv. Mater."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/bs.apcsb.2015.08.002","article-title":"Quartz-crystal microbalance (qcm) for public health: An overview of its applications","volume":"101","author":"Bragazzi","year":"2015","journal-title":"Adv. Protein Chem. Struct. Biol."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"301","DOI":"10.3390\/bios4030301","article-title":"Piezoelectric biosensors for organophosphate and carbamate pesticides: A review","volume":"4","author":"Marrazza","year":"2014","journal-title":"Biosensors"},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"754","DOI":"10.1002\/jmr.1117","article-title":"A survey of the 2006\u20132009 quartz crystal microbalance biosensor literature","volume":"24","author":"Becker","year":"2011","journal-title":"J. Mol. Recognit."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"8082","DOI":"10.20964\/2017.09.67","article-title":"Sensors based on molecularly imprinted polymers","volume":"12","author":"Pohanka","year":"2017","journal-title":"Int. J. Electrochem. Sci."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"2137","DOI":"10.1016\/j.bios.2008.11.016","article-title":"A qcm-d-based biosensor for E. coli o157:H7 highlighting the relevance of the dissipation slope as a transduction signal","volume":"24","author":"Poitras","year":"2009","journal-title":"Biosens. Bioelectron."},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"1330","DOI":"10.1016\/j.bios.2008.07.071","article-title":"Rapid detection of bacillus anthracis using monoclonal antibody functionalized qcm sensor","volume":"24","author":"Hao","year":"2009","journal-title":"Biosens. Bioelectron."},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"341","DOI":"10.3390\/s7030341","article-title":"Rapid characterization of monoclonal antibodies using the piezoelectric immunosensor","volume":"7","author":"Pohanka","year":"2007","journal-title":"Sensors"},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"981","DOI":"10.1016\/j.talanta.2006.05.074","article-title":"Diagnosis of tularemia using piezoelectric biosensor technology","volume":"71","author":"Pohanka","year":"2007","journal-title":"Talanta"},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"2825","DOI":"10.3390\/s7112825","article-title":"Piezoelectric biosensor for a simple serological diagnosis of tularemia in infected european brown hares (Lepus europaeus)","volume":"7","author":"Pohanka","year":"2007","journal-title":"Sensors"},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1016\/j.msec.2018.11.060","article-title":"Carbon dots: The next generation platform for biomedical applications","volume":"96","author":"Ghosal","year":"2019","journal-title":"Mater. Sci. Eng. C Mater. Biol. Appl."},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"616","DOI":"10.3389\/fgene.2018.00616","article-title":"Quantum dot based nano-biosensors for detection of circulating cell free mirnas in lung carcinogenesis: From biology to clinical translation","volume":"9","author":"Singh","year":"2018","journal-title":"Front. Genet."},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/j.trac.2018.07.010","article-title":"Magnetic particles-enabled biosensors for point-of-care testing","volume":"106","author":"Xianyu","year":"2018","journal-title":"Trac-Trends Anal. Chem."},{"key":"ref_103","doi-asserted-by":"crossref","unstructured":"Chen, Y.T., Kolhatkar, A.G., Zenasni, O., Xu, S., and Lee, T.R. (2017). Biosensing using magnetic particle detection techniques. Sensors, 17.","DOI":"10.3390\/s17102300"},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.bios.2018.10.038","article-title":"Recent advances in microfluidic paper-based electrochemiluminescence analytical devices for point-of-care testing applications","volume":"126","author":"Chinnadayyala","year":"2019","journal-title":"Biosens. Bioelectron."},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1016\/j.jpba.2018.07.021","article-title":"Recent progress on the sensitive detection of cardiovascular disease markers by electrochemical-based biosensors","volume":"159","author":"Bakirhan","year":"2018","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1016\/j.bios.2019.05.014","article-title":"Progress in biosensor based on DNA-templated copper nanoparticles","volume":"137","author":"Qing","year":"2019","journal-title":"Biosens. Bioelectron."},{"key":"ref_107","doi-asserted-by":"crossref","unstructured":"Stine, K.J. (2019). Biosensor applications of electrodeposited nanostructures. Appl. Sci., 9.","DOI":"10.3390\/app9040797"},{"key":"ref_108","doi-asserted-by":"crossref","first-page":"159","DOI":"10.2174\/1573411014666180320111246","article-title":"Development of novel nanocomposites based on graphene\/graphene oxide and electrochemical sensor applications","volume":"15","author":"Yola","year":"2019","journal-title":"Curr. Anal. Chem."},{"key":"ref_109","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/j.coelec.2018.05.021","article-title":"Gold nanoparticles in bioelectrocatalysis\u2014The role of nanoparticle size","volume":"12","author":"Kizling","year":"2018","journal-title":"Curr. Opin. Electrochem."},{"key":"ref_110","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.aca.2019.04.014","article-title":"A novel fluorescence aptasensor based on mesoporous silica nanoparticles for selective and sensitive detection of aflatoxin b-1","volume":"1068","author":"Tan","year":"2019","journal-title":"Anal. Chim. Acta"},{"key":"ref_111","doi-asserted-by":"crossref","first-page":"20914","DOI":"10.1039\/C8NR06156D","article-title":"Metal enhanced fluorescence biosensing: From ultra-violet towards second near-infrared window","volume":"10","author":"Fothergill","year":"2018","journal-title":"Nanoscale"},{"key":"ref_112","doi-asserted-by":"crossref","first-page":"568","DOI":"10.1007\/s00604-018-3088-8","article-title":"Diagnosis of hepatitis via nanomaterial-based electrochemical, optical or piezoelectrical biosensors: A review on recent advancements","volume":"185","author":"Hassanpour","year":"2018","journal-title":"Microchim. Acta"},{"key":"ref_113","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1016\/j.mtchem.2019.04.005","article-title":"Aptamer-based biosensors: A novel toolkit for early diagnosis of cancer","volume":"12","author":"Mehmood","year":"2019","journal-title":"Mater. Today Chem."},{"key":"ref_114","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.coelec.2018.11.008","article-title":"Electrochemical aptamer-based assays coupled to isothermal nucleic acid amplification techniques: New tools for cancer diagnosis","volume":"14","year":"2019","journal-title":"Curr. Opin. Electrochem."},{"key":"ref_115","doi-asserted-by":"crossref","unstructured":"Zhang, Y., Lai, B.S., and Juhas, M. (2019). Recent advances in aptamer discovery and applications. Molecules, 24.","DOI":"10.3390\/molecules24050941"},{"key":"ref_116","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1016\/j.jsps.2018.11.013","article-title":"Aptamer based nanobiosensors: Promising healthcare devices","volume":"27","author":"Hanif","year":"2019","journal-title":"Saudi Pharm. J."}],"container-title":["Materials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1996-1944\/12\/14\/2303\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T13:07:07Z","timestamp":1760188027000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1996-1944\/12\/14\/2303"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,7,18]]},"references-count":116,"journal-issue":{"issue":"14","published-online":{"date-parts":[[2019,7]]}},"alternative-id":["ma12142303"],"URL":"https:\/\/doi.org\/10.3390\/ma12142303","relation":{},"ISSN":["1996-1944"],"issn-type":[{"value":"1996-1944","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,7,18]]}}}