{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,11]],"date-time":"2025-12-11T20:53:09Z","timestamp":1765486389098,"version":"build-2065373602"},"reference-count":34,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2019,12,23]],"date-time":"2019-12-23T00:00:00Z","timestamp":1577059200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Materials"],"abstract":"<jats:p>In this study, we described the development of a simplified wet spinning method of the production of a novel type of porous continuous fiber based on poly-3-(R)-hydroxybutyrate (PHB). The principle of this method is precipitation of PHB dissolved in chloroform solution into the ethanol precipitation bath. The influence of various PHB concentrations and feed rates on specific surface area (measured by nitrogen absorption method) was studied. Materials were also characterized by SEM. Surface areas of fibers achieved by wet spinning were in the range of tens of m2.g\u22121, and the biggest surface area value was 55 m2.g\u20131. The average diameter of fibers was in the range of 20\u2013120 \u03bcm and was dependent on both PHB concentration and feed rate. Optimum conditions for reaching stable fibers of high surface area were 3\u20135 % w.t. of PHB and feed rate 0.5\u20133 ml.h\u22121. Fibers were functionalized by adsorption of some natural plant extracts. The incorporation of active substances into fibers was confirmed by infrared spectroscopy. High antioxidant and antimicrobial effect of PHB-fibers with cloves extract was found, as well as excellent long-term stability and optimal dynamics of the release of active compounds. The newly produced material would be applicable in pharmacy, cosmetics, and wound healing.<\/jats:p>","DOI":"10.3390\/ma13010086","type":"journal-article","created":{"date-parts":[[2019,12,24]],"date-time":"2019-12-24T05:56:15Z","timestamp":1577166975000},"page":"86","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Facile Preparation of Porous Microfiber from Poly-3-(R)-Hydroxybutyrate and Its Application"],"prefix":"10.3390","volume":"13","author":[{"given":"Vojtech","family":"Kundrat","sequence":"first","affiliation":[{"name":"Department of Food Science and Biotechnology, Faculty of Chemistry, Brno University of Technology, Brno 612 00, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4705-7752","authenticated-orcid":false,"given":"Petra","family":"Matouskova","sequence":"additional","affiliation":[{"name":"Department of Food Science and Biotechnology, Faculty of Chemistry, Brno University of Technology, Brno 612 00, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivana","family":"Marova","sequence":"additional","affiliation":[{"name":"Department of Food Science and Biotechnology, Faculty of Chemistry, Brno University of Technology, Brno 612 00, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,12,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1007\/s12393-018-9180-3","article-title":"Biodegradable Films for Fruits and Vegetable Packaging Application: Preparation and Properties","volume":"10","author":"Banerjee","year":"2018","journal-title":"Food Eng. Rev."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1503","DOI":"10.1016\/S0079-6700(00)00035-6","article-title":"Synthesis, structure, and properties of polyhydroxyalkanoates: Biological polyesters","volume":"25","author":"Sudesh","year":"2000","journal-title":"Prog. Polym. Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"6565","DOI":"10.1016\/j.biomaterials.2005.04.036","article-title":"The application of polyhydroxyalkanoates as tissue engineering materials","volume":"26","author":"Chen","year":"2005","journal-title":"Biomaterials"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2434","DOI":"10.1039\/b812677c","article-title":"A microbial polyhydroxyalkanoates (PHA) based bio- and materials industry","volume":"38","author":"Chen","year":"2009","journal-title":"Chem. Soc. Rev."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1002\/jctb.1667","article-title":"Polyhydroxyalkanoates: Biodegradable polymers with a range of applications","volume":"82","author":"Philip","year":"2007","journal-title":"J. Chem. Technol. Biotechnol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1186\/2191-0855-1-11","article-title":"Production of polyhydroxyalkanoates from waste frying oil by Cupriavidus necator","volume":"1","author":"Verlinden","year":"2011","journal-title":"AMB Express"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1016\/j.rser.2013.06.014","article-title":"Biodiesel production from used cooking oil: A review","volume":"27","author":"Dupas","year":"2013","journal-title":"Renew. Sustain. Energy Rev."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1016\/j.progpolymsci.2006.03.002","article-title":"Polymer blends and composites from renewable resources","volume":"31","author":"Yu","year":"2006","journal-title":"Prog. Polym. Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/S0960-8524(02)00110-4","article-title":"Preparation and characterization of biodegradable poly-3-hydroxybutyrate\u2013starch blend films","volume":"86","author":"Godbole","year":"2003","journal-title":"Bioresour. Technol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1753","DOI":"10.1016\/j.compscitech.2006.11.009","article-title":"Wood fiber reinforced bacterial bioplastic composites: Fabrication and performance evaluation","volume":"67","author":"Singh","year":"2007","journal-title":"Compos. Sci. Technol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2249","DOI":"10.1021\/bm060317c","article-title":"Polyhydroxyalkanoate (PHA)\/Inorganic Phase Composites for Tissue Engineering Applications","volume":"7","author":"Misra","year":"2006","journal-title":"Biomacromolecules"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2695","DOI":"10.1021\/ja00086a001","article-title":"Amorphous, biomimetic granules of polyhydroxybutyrate: Preparation, characterization, and biological implications","volume":"116","author":"Horowitz","year":"1994","journal-title":"J. Am. Chem. Soc."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1263\/jbb.100.43","article-title":"A composite of hydroxyapatite with electrospun biodegradable nanofibers as a tissue engineering material","volume":"100","author":"Ito","year":"2005","journal-title":"J. Biosci. Bioeng."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"3241","DOI":"10.1016\/S0032-3861(00)00598-X","article-title":"Mechanical properties and higher order structure of bacterial homo poly(3-hydroxybutyrate) melt spun fibers","volume":"42","author":"Yamane","year":"2001","journal-title":"Polymer"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1016\/j.seppur.2015.09.037","article-title":"Air filtration and antimicrobial capabilities of electrospun PLA\/PHB containing ionic liquid","volume":"154","author":"Nicosia","year":"2015","journal-title":"Sep. Purif. Technol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1023\/A:1010124205584","article-title":"PHB\/V in Extrusion Coating of Paper and Paperboard: Part I: Study of Functional Properties","volume":"8","author":"Kuusipalo","year":"2000","journal-title":"J. Polym. Environ."},{"key":"ref_17","first-page":"794","article-title":"Nanocomposite Microcapsules from Powders of Polyhydroxybutyrate (PHB) and Smectite Clays","volume":"660\u2013661","author":"Wang","year":"2010","journal-title":"Mater. Sci. Forum"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1629","DOI":"10.1016\/j.progpolymsci.2013.05.008","article-title":"Bio-nanocomposites for food packaging applications","volume":"38","author":"Rhim","year":"2013","journal-title":"Prog. Polym. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/10731190802664429","article-title":"Medical Application of Microbial Biopolyesters Polyhydroxyalkanoates","volume":"37","author":"Wu","year":"2009","journal-title":"Artif. Cells Blood Substit. Biotechnol."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Rivera-Briso, A., and Serrano-Aroca, \u00c1. (2018). Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate): Enhancement Strategies for Advanced Applications. Polymers, 10.","DOI":"10.3390\/polym10070732"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/0141-3910(93)90137-8","article-title":"The effect of melt-processing on the degradation of selected polyhydroxyacids: Polylactides, polyhydroxybutyrate, and polyhydroxybutyrate-co-valerates","volume":"40","author":"Gogolewski","year":"1993","journal-title":"Polym. Degrad. Stab."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"938","DOI":"10.1002\/jbm.a.31513","article-title":"Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)\/poly(\u03b5-caprolactone) blends for tissue engineering applications in the form of hollow fibers","volume":"85","author":"Chiono","year":"2008","journal-title":"J. Biomed. Mater. Res. Part A"},{"key":"ref_23","unstructured":"Zhang, D. (2014). Advances in Filament Yarn Spinning of Textiles and Polymers, Woodhead Publishing Ltd.. Association with the Textile Institute."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Qin, Y. (2016). An Overview of Medical Textile Products. Medical Textile Materials, Elsevier. Available online: https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/B9780081006184000029.","DOI":"10.1016\/B978-0-08-100618-4.00002-9"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1002\/term.1897","article-title":"Additive manufacturing of poly[(R)-3-hydroxybutyrate-co-(R)-3-hydroxyhexanoate] scaffolds for engineered bone development","volume":"11","author":"Mota","year":"2017","journal-title":"J. Tissue Eng. Regen. Med."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Kundrat, V., Cernekova, N., Kovalcik, A., Enev, V., and Marova, I. (2019). Drug Release Kinetics of Electrospun PHB Meshes. Materials, 12.","DOI":"10.3390\/ma12121924"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/j.biortech.2013.05.012","article-title":"Industrial wastes as a promising renewable source for production of microbial lipid and direct transesterification of the lipid into biodiesel","volume":"142","author":"Cheirsilp","year":"2013","journal-title":"Bioresour. Technol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.wasman.2019.07.009","article-title":"Extraction, identification and quantification of polyphenols from spent coffee grounds by chromatographic methods and chemometric analyses","volume":"96","author":"Madrid","year":"2019","journal-title":"Waste Manag."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.1016\/S0891-5849(98)00315-3","article-title":"Antioxidant activity applying an improved ABTS radical cation decolorization assay","volume":"26","author":"Re","year":"1999","journal-title":"Free Radic. Biol. Med."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"304","DOI":"10.17113\/ftb.54.03.16.4413","article-title":"Effect of Encapsulation of Antimicrobial Activity of Herbal Extracts with Lysozyme","volume":"54","author":"Matouskova","year":"2016","journal-title":"Food Technol. Biotechnol."},{"key":"ref_31","unstructured":"Meyers, R.A. (2000). Interpretation of Infrared Spectra, A Practical Approach. Encyclopedia of Analytical Chemistry, John Wiley & Sons."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Sepahpour, S., Selamat, J., Abdul Manap, M., Khatib, A., and Abdull Razis, A. (2018). Comparative Analysis of Chemical Composition, Antioxidant Activity and Quantitative Characterization of Some Phenolic Compounds in Selected Herbs and Spices in Different Solvent Extraction Systems. Molecules, 23.","DOI":"10.3390\/molecules23020402"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1751","DOI":"10.1007\/s12221-015-5108-1","article-title":"Preparation and characterization of electrospun antimicrobial fibrous membranes based on polyhydroxybutyrate (PHB)","volume":"16","author":"Fan","year":"2015","journal-title":"Fibers Polym."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Yilmaz, F., Celep, G., and Tetik, G. (2016). Nanofibers in Cosmetics. Nanofiber Research, IntechOpen.","DOI":"10.5772\/64172"}],"container-title":["Materials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1996-1944\/13\/1\/86\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T13:44:53Z","timestamp":1760190293000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1996-1944\/13\/1\/86"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,12,23]]},"references-count":34,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2020,1]]}},"alternative-id":["ma13010086"],"URL":"https:\/\/doi.org\/10.3390\/ma13010086","relation":{},"ISSN":["1996-1944"],"issn-type":[{"type":"electronic","value":"1996-1944"}],"subject":[],"published":{"date-parts":[[2019,12,23]]}}}