{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T17:06:39Z","timestamp":1789923999226,"version":"4.0.1"},"reference-count":36,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2020,9,17]],"date-time":"2020-09-17T00:00:00Z","timestamp":1600300800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Materials"],"abstract":"<jats:p>One of the strategies for seeking new biologically active substances is to modify compounds with potential biological activity. In this paper, 1,2,4-triazolin-5-thione derivative (3) was obtained in the cyclization reaction of appropriate thiosemicarbazide (2) as an organic ligand. The copper(II) complex, [CuCl2(H2O)2L2] (L=4-cyclohexyl-3-(nitrophenyl)methyl-1,2,4-triazolin-5-thione) (Cu-3) was prepared in a reaction of free ligand (3) with a CuCl2\u00b72H2O solution in MeOH\/EtOH mixture at room temperature. TGA data show that Cu-3 and free ligand are stable at room temperature. Both compounds were screened in vitro for antibacterial and antifungal activities using the broth microdilution method. The obtained complex (Cu-3) showed higher antibacterial effect, especially towards Gram-positive bacteria (with moderate activity and Minimal Inhibitory Concentration MIC = 250\u2013500 \u00b5g\/mL) than the free ligand (3) (with mild or no bioactivity and MIC \u2265 1000 \u00b5g\/mL). In turn, yeasts, belonging to Candida albicans, exhibited similar sensitivity to both the copper(II) complex (Cu-3) and the organic ligand (3). The anticandidal activity of these compounds was moderate (MIC = 500 \u00b5g\/mL), or, in the case of other Candida spp., lower (MIC \u2265 1000 \u00b5g\/mL).<\/jats:p>","DOI":"10.3390\/ma13184135","type":"journal-article","created":{"date-parts":[[2020,9,17]],"date-time":"2020-09-17T08:29:43Z","timestamp":1600331383000},"page":"4135","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Synthesis, Spectral, Thermal and Biological Studies of 4-Cyclohexyl-3-(4-nitrophenyl)methyl-1,2,4-triazolin-5-thione and Its Copper(II) Coordination Compound, [CuCl2(H2O)2L2]"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7157-5408","authenticated-orcid":false,"given":"Agnieszka","family":"Czylkowska","sequence":"first","affiliation":[{"name":"Institute of General and Ecological Chemistry, Faculty of Chemistry, Lodz University of Technology, Zeromskiego 116, 90-924 Lodz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monika","family":"Drozd","sequence":"additional","affiliation":[{"name":"Independent Radiopharmacy Unit, Faculty of Pharmacy, Medical University of Lublin, Chodzki 4A, 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2844-9876","authenticated-orcid":false,"given":"Anna","family":"Biernasiuk","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Microbiology with the Laboratory of Microbiological Diagnostics, Faculty of Pharmacy, Medical University of Lublin, Chodzki 4A, 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bart\u0142omiej","family":"Rogalewicz","sequence":"additional","affiliation":[{"name":"Institute of General and Ecological Chemistry, Faculty of Chemistry, Lodz University of Technology, Zeromskiego 116, 90-924 Lodz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1503-7634","authenticated-orcid":false,"given":"Anna","family":"Malm","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Microbiology with the Laboratory of Microbiological Diagnostics, Faculty of Pharmacy, Medical University of Lublin, Chodzki 4A, 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8792-1029","authenticated-orcid":false,"given":"Monika","family":"Pitucha","sequence":"additional","affiliation":[{"name":"Independent Radiopharmacy Unit, Faculty of Pharmacy, Medical University of Lublin, Chodzki 4A, 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,9,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"366","DOI":"10.4236\/aim.2013.34050","article-title":"Synthesis and in vitro biological activities of 4,5-disubstituted 1,2,4-triazole-3-thiols","volume":"3","author":"Nadeem","year":"2013","journal-title":"Adv. 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