{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T23:45:30Z","timestamp":1767915930829,"version":"3.49.0"},"reference-count":49,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2021,7,20]],"date-time":"2021-07-20T00:00:00Z","timestamp":1626739200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Materials"],"abstract":"<jats:p>This study involved the preparation and characterization of structures with a honeycomb-like pattern (HCP) formed using the phase separation method using a solution mixture of chloroform and methanol together with cellulose acetate. Fluorinated ethylene propylene modified by plasma treatment was used as a suitable substrate for the formation of the HCP structures. Further, we modified the HCP structures using silver sputtering (discontinuous Ag nanoparticles) or by adding Ag nanoparticles in PEG into the cellulose acetate solution. The material morphology was then determined using atomic force microscopy (AFM) and scanning electron microscopy (SEM), while the material surface chemistry was studied using energy dispersive spectroscopy (EDS) and wettability was analyzed with goniometry. The AFM and SEM results revealed that the surface morphology of pristine HCP with hexagonal pores changed after additional sample modification with Ag, both via the addition of nanoparticles and sputtering, accompanied with an increase in the roughness of the PEG-doped samples, which was caused by the high molecular weight of PEG and its gel-like structure. The highest amount (approx. 25 at %) of fluorine was detected using the EDS method on the sample with an HCP-like structure, while the lowest amount (0.08%) was measured on the PEG + Ag sample, which revealed the covering of the substrate with biopolymer (the greater fluorine extent means more of the fluorinated substrate is exposed). As expected, the thickness of the Ag layer on the HCP surface depended on the length of sputtering (either 150 s or 500 s). The sputtering times for Ag (150 s and 500 s) corresponded to layers with heights of about 8 nm (3.9 at % of Ag) and 22 nm (10.8 at % of Ag), respectively. In addition, we evaluated the antibacterial potential of the prepared substrate using two bacterial strains, one Gram-positive of S. epidermidis and one Gram-negative of E. coli. The most effective method for the construction of antibacterial surfaces was determined to be sputtering (150 s) of a silver nanolayer onto a HCP-like cellulose structure, which proved to have excellent antibacterial properties against both G+ and G\u2212 bacterial strains.<\/jats:p>","DOI":"10.3390\/ma14144051","type":"journal-article","created":{"date-parts":[[2021,7,20]],"date-time":"2021-07-20T11:26:10Z","timestamp":1626780370000},"page":"4051","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Antibacterial Properties of a Honeycomb-like Pattern with Cellulose Acetate and Silver Nanoparticles"],"prefix":"10.3390","volume":"14","author":[{"given":"Klaudia","family":"Hurtukov\u00e1","sequence":"first","affiliation":[{"name":"Department of Solid State Engineering, University of Chemistry and Technology Prague, Technick\u00e1 3, 166 28 Prague, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kl\u00e1ra","family":"Fajstavrov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Solid State Engineering, University of Chemistry and Technology Prague, Technick\u00e1 3, 166 28 Prague, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3008-1396","authenticated-orcid":false,"given":"Silvie","family":"Rimpelov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Microbiology, University of Chemistry and Technology Prague, Technick\u00e1 3, 166 28 Prague, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbora","family":"Vokat\u00e1","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Microbiology, University of Chemistry and Technology Prague, Technick\u00e1 3, 166 28 Prague, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dominik","family":"Fajstavr","sequence":"additional","affiliation":[{"name":"Department of Solid State Engineering, University of Chemistry and Technology Prague, Technick\u00e1 3, 166 28 Prague, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nikola Slepi\u010dkov\u00e1","family":"Kas\u00e1lkov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Solid State Engineering, University of Chemistry and Technology Prague, Technick\u00e1 3, 166 28 Prague, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9961-1447","authenticated-orcid":false,"given":"Jakub","family":"Siegel","sequence":"additional","affiliation":[{"name":"Department of Solid State Engineering, University of Chemistry and Technology Prague, Technick\u00e1 3, 166 28 Prague, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V\u00e1clav","family":"\u0160vor\u010d\u00edk","sequence":"additional","affiliation":[{"name":"Department of Solid State Engineering, University of Chemistry and Technology Prague, Technick\u00e1 3, 166 28 Prague, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Petr","family":"Slepi\u010dka","sequence":"additional","affiliation":[{"name":"Department of Solid State Engineering, University of Chemistry and Technology Prague, Technick\u00e1 3, 166 28 Prague, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,7,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1016\/j.pmatsci.2015.05.001","article-title":"Bioinspired engineering of honeycomb structure\u2014Using nature to inspire human innovation","volume":"74","author":"Zhang","year":"2015","journal-title":"Prog. Mater. Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/B978-0-12-409547-2.12641-1","article-title":"Honeycomb films with ordered patterns and structures","volume":"Volume 9","author":"Dong","year":"2017","journal-title":"Comprehensive Supramolecular Chemistry II"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"5052","DOI":"10.1039\/C9SM00845D","article-title":"Direct formation of hydrophilic honeycomb film by self-assembly in breath figure templating of hydrophobic polylacticacid\/ionic surfactant complexes","volume":"15","author":"Yin","year":"2019","journal-title":"Soft Mater."},{"key":"ref_4","unstructured":"Bencsik, M., and Ramsey, M. (2020, March 15). We Discovered More about the Honeybee \u2018Wake-Up Call\u2019\u2014And It Could Help Save Them, the Conversation. Available online: https:\/\/theconversation.com\/we-discovered-more-about-the-honeybee-wake-up-call-and-it-could-help-save-them-105751."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1900063","DOI":"10.1002\/ppap.201900063","article-title":"Honeycomb-like pattern formation on perfluoroethylenepropylene enhanced by plasma treatment","volume":"16","author":"Fajstavr","year":"2019","journal-title":"Plasma Process Polym."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1016\/j.jscs.2020.01.002","article-title":"Advances in the scaffolds fabrication techniques using biocompatible polymers and their biomedical application: A technical and statistical review","volume":"24","author":"Haider","year":"2020","journal-title":"J. Saudi Chem. Soc."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Asadi, N., Del Bakhshayesh, A.R., Davaran, S., and Akbarzadeh, A. (2019). Common Biocompatible Polymeric Materials for Tissue Engineering and Regenerative Medicine. Mater. Chem. Phys., 122528.","DOI":"10.1016\/j.matchemphys.2019.122528"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"110713","DOI":"10.1016\/j.colsurfb.2019.110713","article-title":"Electrospun cellulose acetate butyrate\/polyethylene glycol (CAB\/PEG) composite nanofibers: A potential scaffold for tissue engineering","volume":"188","author":"Tan","year":"2020","journal-title":"Colloids Surfaces B Biointerfaces"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.actbio.2017.11.043","article-title":"Breath figures in tissue engineering and drug delivery: State-of-the-art and future perspectives","volume":"66","author":"Calejo","year":"2018","journal-title":"Acta Biomater."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"4384","DOI":"10.1016\/j.msec.2013.06.036","article-title":"Creating \u201chotels\u201d for cells by electrospinning honeycomb-like polymeric structures","volume":"33","author":"Liang","year":"2013","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1016\/j.polymer.2017.05.038","article-title":"Coupling of breath figure method with interfacial polymerization: Bottom-surface functionalized honeycomb-patterned porous films","volume":"119","author":"Male","year":"2017","journal-title":"Polymer"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1016\/j.progpolymsci.2013.08.006","article-title":"Towards hierarchically ordered functional porous polymeric surfaces prepared by the breath figures approach","volume":"39","year":"2014","journal-title":"Prog. Polym. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"10541","DOI":"10.1021\/acsami.5b02097","article-title":"Large-Scale Fabrication of Commercially Available, Nonpolar Linear Polymer Film with a Highly Ordered Honeycomb Pattern","volume":"7","author":"Bui","year":"2015","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"137503","DOI":"10.1016\/j.tsf.2019.137503","article-title":"Fabrication of hierarchically structured surfaces with \u201crose petal\u201d effect by a modified breath figure method","volume":"689","author":"Dong","year":"2019","journal-title":"Thin Solid Films"},{"key":"ref_15","first-page":"100927","article-title":"3D printed porous cellulose acetate tissue scaffolds for additive manufacturing","volume":"31","author":"Huang","year":"2020","journal-title":"Addit. Manuf."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.carbpol.2018.02.061","article-title":"Multi-hierarchical tissue-engineering ECM-like scaffolds based on cellulose acetate with collagen and chitosan fillers","volume":"191","author":"Lukanina","year":"2018","journal-title":"Carbohydr. Polym."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"115628","DOI":"10.1016\/j.carbpol.2019.115628","article-title":"The role of physicochemical properties in the nanoprecipitation of cellulose acetate","volume":"230","author":"Ghasemi","year":"2020","journal-title":"Carbohydr. Polym."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1103","DOI":"10.1016\/j.msec.2016.08.015","article-title":"Crosslinked pullulan\/cellulose acetate fibrous scaffolds for bone tissue engineering","volume":"69","author":"Atila","year":"2016","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"107978","DOI":"10.1016\/j.carres.2020.107978","article-title":"A review on the properties of electrospun cellulose acetate and its application in drug delivery systems: A new perspective","volume":"491","author":"Wsoo","year":"2020","journal-title":"Carbohydr. Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1515\/polyeng-2016-0272","article-title":"Cellulose modification and shaping\u2014A review","volume":"37","author":"Kerstin","year":"2017","journal-title":"J. Polym. Eng."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/j.carbpol.2015.06.109","article-title":"Cellulose acetate based 3-dimensional electrospun scaffolds for skin tissue engineering applications","volume":"133","author":"Atila","year":"2015","journal-title":"Carbohydr. Polym."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"4639","DOI":"10.1016\/j.biomaterials.2010.02.044","article-title":"Bioactive modification of poly(ethylene glycol) hydrogels for tissue engineering","volume":"31","author":"Zhu","year":"2010","journal-title":"Biomaterials"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1007\/s10856-016-5793-3","article-title":"Hydrogels based on poly(ethylene glycol) as scaffolds for tissue engineering application: Biocompatibility assessment and effect of the sterilization process","volume":"27","year":"2016","journal-title":"J. Mater. Sci. Mater. Med."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1002\/1097-4636(20000905)51:3<343::AID-JBM7>3.0.CO;2-D","article-title":"Polyethylene glycol-coated biocompatible surfaces","volume":"51","author":"Alcantar","year":"2000","journal-title":"J. Biomed. Mater. Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"4200","DOI":"10.1002\/adma.200801170","article-title":"Preparation of highly oriented nano-pit arrays by thermal shrinking of honeycomb-patterned polymer films","volume":"20","author":"Yabu","year":"2008","journal-title":"Adv. Mater."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1002\/masy.200950527","article-title":"Fabrication of ordered arrays of biodegradable polymer pincushions using self-organized honeycomb-patterned films","volume":"279","author":"Tanaka","year":"2009","journal-title":"Macromol. Symp."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Cardoso, V.F., Correia, D.M., Ribeiro, C., Fernandes, M.M., and Lanceros-M\u00e9ndez, S. (2018). Fluorinated polymers as smart materials for advanced biomedical applications. Polymers, 10.","DOI":"10.3390\/polym10020161"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"923","DOI":"10.1016\/j.ijbiomac.2018.01.089","article-title":"Nano-biocomposite scaffolds of chitosan, carboxymethyl cellulose and silver nanoparticle modified cellulose nanowhiskers for bone tissue engineering applications","volume":"111","author":"Hasan","year":"2018","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1123","DOI":"10.1007\/s10068-017-0138-7","article-title":"Antimicrobial effect of PEG-PLA on food-spoilage microorganisms","volume":"26","author":"Sahan","year":"2017","journal-title":"Food Sci. Biotechnol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"11935","DOI":"10.1021\/acsomega.9b04064","article-title":"Pyramid-Shaped PEG-PCL-PEG Polymeric-Based Model Systems for Site-Specific Drug Delivery of Vancomycin with Enhance Antibacterial Efficacy","volume":"5","author":"Singh","year":"2020","journal-title":"ACS Omega"},{"key":"ref_31","first-page":"6526","article-title":"Photoinduced chitosan-PEG hydrogels with long-term antibacterial properties","volume":"7","author":"Razza","year":"2018","journal-title":"J. Mater. Chem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"3311","DOI":"10.2147\/IJN.S165125","article-title":"Potential antibacterial mechanism of silver nanoparticles and the optimization of orthopedic implants by advanced modification technologies","volume":"13","author":"Qing","year":"2018","journal-title":"Int. J. Nanomed."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2555","DOI":"10.2147\/IJN.S246764","article-title":"The Antibacterial Mechanism of Silver Nanoparticles and Its Application in Dentistry","volume":"15","author":"Yin","year":"2020","journal-title":"Int. J. Nanomed."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"20676","DOI":"10.1039\/D0RA03240A","article-title":"Evaluation of the antimicrobial activity of silver nanoparticles obtained by microwave-assisted green synthesis using Handroanthus impetiginosus (Mart. ex DC.) Mattos underbark extract","volume":"10","author":"Tormena","year":"2020","journal-title":"RSC Adv."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1469","DOI":"10.2147\/IJN.S191340","article-title":"Antibacterial activity and mechanism of silver nanoparticles against multidrug-resistant Pseudomonas aeruginosa","volume":"14","author":"Liao","year":"2019","journal-title":"Int. J. Nanomed."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Jayakumar, R., and Nair, S. (2012). Biomedical Applications of Polymer\/Silver Composite Nanofibers. Biomedical Applications of Polymeric Nanofibers, Springer.","DOI":"10.1007\/978-3-642-27148-9"},{"key":"ref_37","first-page":"614","article-title":"Chemick\u00e9 a fyzik\u00e1ln\u00ed modifikace biomateri\u00e1l\u016f na b\u00e1zi celulosy","volume":"111","year":"2017","journal-title":"Chem. Listy"},{"key":"ref_38","first-page":"197","article-title":"Surface modification of biopolymers by argon plasma and thermal treatment","volume":"9","author":"Sajdl","year":"2011","journal-title":"Plasma Process. Polym."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1078","DOI":"10.3144\/expresspolymlett.2020.87","article-title":"Cellulose acetate honeycomb-like pattern created by improved phase separation","volume":"14","author":"Sajdl","year":"2020","journal-title":"Express Polym. Lett."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Baillot, R., and Deshayes, Y. (2017). Tools and analysis methods of encapsulated LEDs. Reliability Investigation of LED Devices for Public Light Applications, Elsevier.","DOI":"10.1016\/B978-1-78548-149-9.50002-X"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"033005","DOI":"10.1088\/1367-2630\/10\/3\/033005","article-title":"Formation and properties of metal-oxygen atomic chains","volume":"10","author":"Thijssen","year":"2008","journal-title":"New J. Phys."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/j.carbpol.2012.12.037","article-title":"Electrospun nanofibrous cellulose scaffolds with controlled microarchitecture","volume":"100","author":"Sundberg","year":"2014","journal-title":"Carbohydr. Polym."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1016\/j.culher.2016.03.005","article-title":"ATR-FTIR characterization of old pressure sensitive adhesive tapes in historic papers","volume":"21","author":"Gorassinia","year":"2016","journal-title":"J. Cult. Herit."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1007\/s11664-009-1016-8","article-title":"A study of plasma-cleaned Ag-plated Cu leadframe surfaces","volume":"39","author":"Li","year":"2010","journal-title":"J. Electron. Mater."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Sim, W., Barnard, R.T., Blaskovich, M.A.T., and Ziora, Z.M. (2018). Antimicrobial silver in medicinal and consumer applications: A patent review of the past decade (2007\u20132017). Antibiotics, 7.","DOI":"10.3390\/antibiotics7040093"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1016\/j.colsurfb.2015.08.010","article-title":"Control of bacterial adhesion and growth on honeycomb-like patterned surfaces","volume":"135","author":"Yang","year":"2015","journal-title":"Colloids Surfaces B Biointerfaces"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1016\/j.msec.2016.04.052","article-title":"Antibacterial properties of modified biodegradable PHB non-woven fabric","volume":"65","year":"2016","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1016\/j.tibtech.2010.07.006","article-title":"Nanosilver as a new generation of nanoproduct in biomedical applications","volume":"28","author":"Chaloupka","year":"2010","journal-title":"Trends Biotechnol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1007\/s11051-014-2850-z","article-title":"Stabilization of sputtered gold and silver nanoparticles in PEG colloid solutions","volume":"17","author":"Elashnikov","year":"2015","journal-title":"J. Nanoparticles Res."}],"container-title":["Materials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1996-1944\/14\/14\/4051\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T06:32:13Z","timestamp":1760164333000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1996-1944\/14\/14\/4051"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,7,20]]},"references-count":49,"journal-issue":{"issue":"14","published-online":{"date-parts":[[2021,7]]}},"alternative-id":["ma14144051"],"URL":"https:\/\/doi.org\/10.3390\/ma14144051","relation":{},"ISSN":["1996-1944"],"issn-type":[{"value":"1996-1944","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,7,20]]}}}