{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T23:42:52Z","timestamp":1772149372758,"version":"3.50.1"},"reference-count":29,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2021,8,24]],"date-time":"2021-08-24T00:00:00Z","timestamp":1629763200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Materials"],"abstract":"<jats:p>This work aimed to assess the impact of acellularization and sterilization methods on the mechanical properties of biocomposites used as a skin substitute. On the basis of the statistical analysis, it was ascertained that the values of the Young modulus for the samples before the sterilization process\u2014only in the cases of substances such as: trypsin, 15% glycerol and dispase\u2014changed in a statistically significant way. In the case of dispase, the Young modulus value before the sterilization process amounted to 66.6 MPa, for trypsin this value equalled 33.9 MPa, whereas for 15% glycerol it was 11 MPa. In the case of samples after the completion of the sterilization process, the analysis did not show any statistically significant differences between the obtained results of Young\u2019s modulus depending on the respective reagents applied. It was confirmed that different methods of acellularization and the process of sterilization effect the alteration of mechanical properties of allogeneic skins. In the case of the decellularization method using SDS (Sodium Dodecyl Sulfate), liquid nitrogen and 85% glycerol the highest values of strain were observed. In the authors\u2019 opinion, it is the above-mentioned methods that should be recommended in the process of preparation of skin substitutes.<\/jats:p>","DOI":"10.3390\/ma14174785","type":"journal-article","created":{"date-parts":[[2021,8,24]],"date-time":"2021-08-24T22:12:22Z","timestamp":1629843142000},"page":"4785","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Assessment of the Impact of Decellularization Methods on Mechanical Properties of Biocomposites Used as Skin Substitute"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4286-001X","authenticated-orcid":false,"given":"Bo\u017cena","family":"Gzik-Zroska","sequence":"first","affiliation":[{"name":"Department of Biomaterials and Medical Devices Engineering, Faculty of Biomedical Engineering, Silesian University of Technology, Roosevelta 40 Str., 41-800 Zabrze, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8229-3032","authenticated-orcid":false,"given":"Kamil","family":"Joszko","sequence":"additional","affiliation":[{"name":"Department of Biomechatronics, Faculty of Biomedical Engineering, Silesian University of Technology, Roosevelta 40 Str., 41-800 Zabrze, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4553-3479","authenticated-orcid":false,"given":"Wojciech","family":"Wola\u0144ski","sequence":"additional","affiliation":[{"name":"Department of Biomechatronics, Faculty of Biomedical Engineering, Silesian University of Technology, Roosevelta 40 Str., 41-800 Zabrze, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S\u0142awomir","family":"Sucho\u0144","sequence":"additional","affiliation":[{"name":"Department of Biomechatronics, Faculty of Biomedical Engineering, Silesian University of Technology, Roosevelta 40 Str., 41-800 Zabrze, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Micha\u0142","family":"Burkacki","sequence":"additional","affiliation":[{"name":"Department of Biomechatronics, Faculty of Biomedical Engineering, Silesian University of Technology, Roosevelta 40 Str., 41-800 Zabrze, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marek","family":"Ples","sequence":"additional","affiliation":[{"name":"Department of Biomechatronics, Faculty of Biomedical Engineering, Silesian University of Technology, Roosevelta 40 Str., 41-800 Zabrze, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1300-8020","authenticated-orcid":false,"given":"Jerzy","family":"Malachowski","sequence":"additional","affiliation":[{"name":"Faculty of Mechanical Engineering, Institute of Mechanics and Computational Engineering, Military University of Technology, Gen. Sylwestra Kaliskiego 2, 00-908 Warszaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6381-6697","authenticated-orcid":false,"given":"Micha\u0142","family":"Tomaszewski","sequence":"additional","affiliation":[{"name":"Faculty of Mechanical Engineering, Institute of Mechanics and Computational Engineering, Military University of Technology, Gen. Sylwestra Kaliskiego 2, 00-908 Warszaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5910-0554","authenticated-orcid":false,"given":"Arkadiusz","family":"Szarek","sequence":"additional","affiliation":[{"name":"Department of Technology and Automation, Faculty of Mechanical Engineering and Computer Science, Czestochowa University of Technology, 21 Armii Krajowej Av., 42-201 Czestochowa, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0240-4055","authenticated-orcid":false,"given":"Grzegorz","family":"Stradomski","sequence":"additional","affiliation":[{"name":"Faculty of Production Engineering and Materials Technology, Czestochowa University of Technology, 19 Armii Krajowej Av., 42-201 Czestochowa, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diana","family":"Kitala","sequence":"additional","affiliation":[{"name":"Stanislaw Sakiel Burn Treatment Centre, Jana Pawla II 2 Str., 41-100 Siemianowice Slaskie, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2902-6557","authenticated-orcid":false,"given":"Mohsen","family":"Akbari","sequence":"additional","affiliation":[{"name":"Laboratory for Innovations in Microengineering (LiME), Department of Mechanical Engineering, University of Victoria, Victoria, BC V8P 5C2, Canada"},{"name":"Centre for Advanced Materials and Related Technologies (CAMTEC), University of Victoria, Victoria, BC V8P 5C2, Canada"},{"name":"Biotechnology Center, Silesian University of Technology, Akademicka 2A, 44-100 Gliwice, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marek","family":"Gzik","sequence":"additional","affiliation":[{"name":"Department of Biomechatronics, Faculty of Biomedical Engineering, Silesian University of Technology, Roosevelta 40 Str., 41-800 Zabrze, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,8,24]]},"reference":[{"key":"ref_1","first-page":"28","article-title":"Chronic wounds and their treatment. 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