{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:53:46Z","timestamp":1787028826504,"version":"build-2736575974"},"reference-count":57,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2022,8,18]],"date-time":"2022-08-18T00:00:00Z","timestamp":1660780800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Materials"],"abstract":"<jats:p>GBR (Guided Bone Regeneration) procedure is challenged by the risk of membrane exposure to the oral cavity and contamination. The barrier quality of these membranes serve as a mechanical block from bacterial penetration into the GBR site. The purpose of this in vitro study was to evaluate the antibacterial effect of three commercial non-resorbable polytetrafluoroethylene membranes. (Two d-PTFE membranes and one double layer e-PTFE +d-PTFE membrane). A validated in vitro model with two bacterial species (Streptococcus sanguinis and Fusobacterium nucleatum) was used. Eight samples from membrane each were placed in a 96-well microtiter plate. The experimental and positive control groups were exposed to a bacterial suspension which involved one bacterial species in each plate. Bacterial growth was monitored spectrophotometrically at 650 nm for 24 h in temperature controlled microplate spectrophotometer under anaerobic conditions. One- Sample Kolmogorov\u2013Smirnov Normal test and the Kruskal\u2013Wallis test was used for the statistical analysis. As shown by the bacterial growth curves obtained from the spectrophotometer readings, all three membranes resulted in bacterial growth. We have not found a statistical difference in F. nucleatum growth between different membrane samples and the positive control group. However, S. sanguinis growth was reduced significantly in the presence of two membranes (CYTOPLAST TXT-200 and NeoGenTM) when compared to the control (p &lt; 0.01). The presence of Permamem\u00ae had no significant influence on S. sanguinis growth. Some types of commercial non-resorbable PTFE membranes may have an impact on the growth dynamics of specific bacterial species.<\/jats:p>","DOI":"10.3390\/ma15165705","type":"journal-article","created":{"date-parts":[[2022,8,18]],"date-time":"2022-08-18T23:28:41Z","timestamp":1660865321000},"page":"5705","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Bacterial Growth on Three Non-Resorbable Polytetrafluoroethylene (PTFE) Membranes\u2014An In Vitro Study"],"prefix":"10.3390","volume":"15","author":[{"given":"Helena","family":"Zelikman","sequence":"first","affiliation":[{"name":"Department of Oral Rehabilitation, Goldschleger School of Dental Medicine, Sackler Medical Faculty, Tel Aviv University, Tel Aviv 6997801, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gil","family":"Slutzkey","sequence":"additional","affiliation":[{"name":"Department of Periodontology and Dental Implantology, Goldschleger School of Dental Medicine, Sackler Medical Faculty, Tel Aviv University, Tel Aviv 6997801, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ofir","family":"Rosner","sequence":"additional","affiliation":[{"name":"Department of Oral Rehabilitation, Goldschleger School of Dental Medicine, Sackler Medical Faculty, Tel Aviv University, Tel Aviv 6997801, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0241-9342","authenticated-orcid":false,"given":"Shifra","family":"Levartovsky","sequence":"additional","affiliation":[{"name":"Department of Oral Rehabilitation, Goldschleger School of Dental Medicine, Sackler Medical Faculty, Tel Aviv University, Tel Aviv 6997801, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9987-0764","authenticated-orcid":false,"given":"Shlomo","family":"Matalon","sequence":"additional","affiliation":[{"name":"Department of Oral Rehabilitation, Goldschleger School of Dental Medicine, Sackler Medical Faculty, Tel Aviv University, Tel Aviv 6997801, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilan","family":"Beitlitum","sequence":"additional","affiliation":[{"name":"Department of Periodontology and Dental Implantology, Goldschleger School of Dental Medicine, Sackler Medical Faculty, Tel Aviv University, Tel Aviv 6997801, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1111\/j.1600-0501.2010.01922.x","article-title":"Guided Bone Regeneration: Biological principle and therapeutic applications","volume":"21","author":"Retzepi","year":"2010","journal-title":"Clin. Oral Implant. Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1111\/j.1600-051X.2008.01269.x","article-title":"Clinical outcomes of implants following lateral bone augmentation: Systematic assessment of available options (barrier membranes, bone grafts, split osteotomy)","volume":"35","author":"Donos","year":"2008","journal-title":"J. Clin. Periodontol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1034\/j.1600-0501.1999.100602.x","article-title":"Adherence of Periodontopathic Bacteria to Biobsorbable and Non-Absorbable Barrier membranes in vitro","volume":"10","author":"Sela","year":"1999","journal-title":"Clin. Oral Implant. Res."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1111\/j.1600-0757.1999.tb00154.x","article-title":"Infectious aspects of periodontal regeneration","volume":"19","author":"Slots","year":"1999","journal-title":"Periodontology 2000"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1902\/jop.1990.61.8.515","article-title":"Scaning electron microscopic observations of cell population and bacterial contamination of membranes used for guided periodontal tissue regeneration in humans","volume":"61","author":"Selvig","year":"1990","journal-title":"J. Periodontol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"974","DOI":"10.1902\/jop.1992.63.12.974","article-title":"Regenerative surgery of intrabony periodontal defects using e-PTFE Barrier membranes: Scanning electron microscopic evaluation of retrieved membranes versus clinical healing","volume":"63","author":"Selvig","year":"1992","journal-title":"J. Periodontol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1902\/jop.1994.65.3.211","article-title":"Adherence of oral microorganisms to guided tissue membranes: An in vitro study","volume":"65","author":"Wang","year":"1994","journal-title":"J. Periodontol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1039","DOI":"10.1111\/j.1600-051X.1996.tb00534.x","article-title":"Bacterial colonization of barrier material and periodontal regeneration","volume":"23","author":"Zuccelli","year":"1996","journal-title":"J. Clin. Periodontol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1111\/j.1600-051X.1994.tb00305.x","article-title":"Microorganisms in PTFE barrier membranes for guided tissue regeneration","volume":"21","author":"Nowzari","year":"1994","journal-title":"J. Clin. Periodontol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1111\/j.1600-051X.1995.tb00179.x","article-title":"Periodontal pathogens on PTFE membrane for guided tissue regeneration inhibit healing","volume":"22","author":"Nowzari","year":"1995","journal-title":"J. Clin. Periodontol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1902\/jop.1994.65.2.154","article-title":"Clinical, microbiological, and histological factors which influence the success of regenerative periodontal therapy","volume":"65","author":"Machtei","year":"1994","journal-title":"J. Periodontol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1097\/ID.0b013e3181cda72c","article-title":"Guided bone regeneration using non-expanded polytetrafluoroethylene mem-branes in preparation for dental implant placements: A report of 420 cases","volume":"19","author":"Barboza","year":"2010","journal-title":"Implant. Dent."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1580","DOI":"10.1902\/jop.2001.72.11.1580","article-title":"A comparison of porous and non-porous Teflon mem-branes plus demineralized freeze-dried bone allograft in the treatment of Class II buccal\/ lingual furcation defects: A clinical reentry study","volume":"72","author":"Lamb","year":"2001","journal-title":"J. Periodontol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1067\/moe.2000.98922","article-title":"Efficacy of high-density versus semipermeable PTFE membranes in an elderly experimental model","volume":"89","author":"Marouf","year":"2000","journal-title":"Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endodontol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1218","DOI":"10.1016\/j.joms.2008.11.022","article-title":"Barrier membranes used for ridge augmentation: Is there an optimal pore size?","volume":"67","author":"Gutta","year":"2009","journal-title":"J. Oral Maxillofac. Surg."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1016\/0142-9612(96)86739-1","article-title":"Effects of different osteopromotive membrane porosities on experimental bone neogenesis in rats","volume":"17","author":"Zellin","year":"1996","journal-title":"Biomaterials"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1034\/j.1600-0501.1998.090406.x","article-title":"Influence of barrier occlusiveness on guided bone augmentation. An experimental study in the rat","volume":"9","author":"Lundgren","year":"1998","journal-title":"Clin. Oral Implant. Res."},{"key":"ref_18","first-page":"859","article-title":"Current barrier membranes: Titanium mesh and other membranes for guided bone regeneration in dental applications","volume":"25","author":"Rakhmatia","year":"2014","journal-title":"J. Prosthodont. Res."},{"key":"ref_19","first-page":"166","article-title":"A comparative study of the effectiveness of e-PTFE membranes with and without early exposure during the healing period","volume":"14","author":"Simion","year":"1994","journal-title":"Int. J. Periodontics Restor. Dent."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1902\/jop.1994.65.8.755","article-title":"A preliminary report on a method for studying the permeability of expanded polytetrafluoroethylene membrane to bacteria in vitro: A scanning electron microscopic and histological study","volume":"65","author":"Simion","year":"1994","journal-title":"J. Periodontol."},{"key":"ref_21","first-page":"62","article-title":"A simplified technique for ridge preservation after tooth extraction","volume":"14","author":"Bartee","year":"1995","journal-title":"Dent. Today"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1097\/00008505-199504000-00004","article-title":"The use of high-density polyte-trafluoroethylene membrane to treat osseous defects: Clinical reports","volume":"4","author":"Bartee","year":"1995","journal-title":"Implant. Dent."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1563\/1548-1336(2001)027<0194:ESRFAR>2.3.CO;2","article-title":"Extraction site reconstruction for alveolar ridge preservation. Part 2: Membrane-assisted surgical technique","volume":"27","author":"Bartee","year":"2001","journal-title":"J. Oral Implantol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1563\/AAID-JOI-D-10-00128","article-title":"Bone regeneration around immediate implants utilizing a dense PTFE membrane without primary closure: A report of 3 cases","volume":"39","author":"Waasdorp","year":"2013","journal-title":"J. Oral Implantol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1355","DOI":"10.1902\/jop.2008.070502","article-title":"Alveolar bone preservation in extraction sockets using non-resorbable dPTFE membranes: A retrospec-tive non-randomized study","volume":"79","author":"Hoffmann","year":"2008","journal-title":"J. Periodontol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"748","DOI":"10.1016\/j.joms.2006.10.042","article-title":"Using a dense PTFE membrane without primary closure to achieve bone and tissue regeneration","volume":"65","author":"Barber","year":"2007","journal-title":"J. Oral Maxillofac. Surg."},{"key":"ref_27","first-page":"7","article-title":"A membrane and graft technique for ridge maintenance using high-density polytetrafluoroethylene (n-PTFE) and hydroxylapatite: Report of four cases","volume":"112","author":"Bartee","year":"1995","journal-title":"Tex. Dent. J."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"401","DOI":"10.4317\/medoral.15.e401","article-title":"Polyurethane, and PTFE mem-branes for guided bone regeneration: Histo-pathological and ultrastructural evaluation","volume":"15","author":"Monteiro","year":"2010","journal-title":"Med. Oral Patol. Oral Cir. Bucal."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.1902\/jop.2003.74.8.1161","article-title":"Comparison of porous and non-porous Teflon membranes plus a xenograft in the treatment of vertical osseous defects: A clinical re-entry study","volume":"74","author":"Walters","year":"2003","journal-title":"J. Periodontol."},{"key":"ref_30","first-page":"1256","article-title":"Evaluation of a new polytetra-fluoroethylene guided tissue regeneration membrane in healing extraction sites","volume":"19","author":"Bartee","year":"1998","journal-title":"Com-Pend. Contin. Educ. Dent."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"253","DOI":"10.5051\/jpis.2011.41.5.253","article-title":"Secondary closure of an extraction socket using the double-membrane guided bone regeneration technique with immediate implant placement","volume":"41","author":"Yun","year":"2011","journal-title":"J. Periodontal Implant. Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1111\/clr.13121","article-title":"Effect of membrane exposure on guided bone regeneration: A systematic review and meta-analysis","volume":"29","author":"Garcia","year":"2018","journal-title":"Clin. Oral Implant. Res."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1186\/1746-160X-4-22","article-title":"In vitro evaluation of various bioabsorbable and nonresorbable barrier membranes for guided tissue regeneration","volume":"4","author":"Kasaj","year":"2008","journal-title":"Head Face Med."},{"key":"ref_34","first-page":"43","article-title":"Collagen barrier membranes may accelerate bacterial growth in vitro: A potential clinical risk to regenerative procedures","volume":"46","author":"Slutzkey","year":"2015","journal-title":"Quintessence Int."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1111\/j.1600-9657.1996.tb00511.x","article-title":"Assessment of antibacterial activity of endodontic sealers by a direct contact test","volume":"12","author":"Weiss","year":"1996","journal-title":"End. Dent. Traumatol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"738","DOI":"10.1111\/cid.12629","article-title":"In vitro evaluation of barrier function against oral bacteria of dense and expanded PTFE membranes for guided bone regeneration","volume":"20","author":"Trobos","year":"2018","journal-title":"Clin. Implant. Dent. Relat. Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1111\/j.1399-302X.1987.tb00298.x","article-title":"The microbiota associated with successful or failing osseointegrated titanium implants","volume":"2","author":"Mombelli","year":"1987","journal-title":"Oral Microbiol. Immunol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1111\/j.1399-302X.1988.tb00095.x","article-title":"Colonization of osseointegrated titanium implants in edentulous patients. Early results","volume":"3","author":"Mombelli","year":"1988","journal-title":"Oral Microbiol. Immunol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1177\/08959374940080021801","article-title":"Mechanisms of dental plaque formation","volume":"8","author":"Scheie","year":"1994","journal-title":"Adv. Dent. Res."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1177\/0022034520949851","article-title":"Microbial community-driven etiopathogenesis of peri-implantitis","volume":"100","author":"Belibasakis","year":"2021","journal-title":"J. Dent. Res."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"752","DOI":"10.1128\/iai.47.3.752-759.1985","article-title":"Surface properties of Streptococcus sanguis FW213 mutants nonadherent to saliva-coated hydroxyapatite","volume":"47","author":"Thompson","year":"1985","journal-title":"Infect. Immun."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"71","DOI":"10.2334\/josnusd.48.71","article-title":"The effect of Piper betle and Psidium guajava extracts on cell-surface hydrophobicity of selected early settlers of dental plaque","volume":"2","author":"Razak","year":"2006","journal-title":"J. Oral Scien"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"915","DOI":"10.2217\/fmb-2018-0043","article-title":"Streptococcus sanguinis biofilm formation & interaction with oral pathogens","volume":"13","author":"Kitten","year":"2018","journal-title":"Future Microbiol."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Nobbs, A., and Kreth, J. (2019). Genetics of sanguinis-group streptococci in health and disease. Microbiol. Spectr., 7.","DOI":"10.1128\/microbiolspec.GPP3-0052-2018"},{"key":"ref_45","first-page":"443","article-title":"Scannapieco, Experimental Salivary Pellicles Formed on Titanium Surfaces Mediate Adhesion of Streptococci Mira","volume":"11","author":"Edgerton","year":"1996","journal-title":"Int. J. Oral Maxillofac. Implant."},{"key":"ref_46","unstructured":"Lindhe, J., Karring, T., and Lang, N.P. (2017). Clinical Periodontology and Implant Dentistry, Wiley. [6th ed.]."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1038\/s41522-020-00155-7","article-title":"Strong oral plaque microbiome signatures for dental implant diseases identified by strain-resolution metagenomics","volume":"6","author":"Ghensi","year":"2002","journal-title":"NPJ Biofilms Microbiomes"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1146\/annurev.micro.54.1.413","article-title":"Oral microbial communities: Biofilms, interactions, and genetic systems","volume":"54","author":"Kolenbrander","year":"2000","journal-title":"Annu. Rev. Microbiol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1034\/j.1600-0501.1996.070415.x","article-title":"The influence of periodontitis on the subgingival flora around implants in partially edentulous patients","volume":"7","author":"Papaioannou","year":"1996","journal-title":"Clin. Oral Implant. Res."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"860149","DOI":"10.3389\/fmicb.2022.860149","article-title":"Fusobacterium nucleatum: The Opportunistic Pathogen of Periodontal and Peri-Implant Diseases","volume":"13","author":"Chen","year":"2022","journal-title":"Front. Microbiol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.mib.2014.11.013","article-title":"Fusobacterium nucleatum: A commensal-turned pathogen","volume":"23","author":"Han","year":"2015","journal-title":"Curr. Opin. Microbiol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"4729","DOI":"10.1128\/IAI.66.10.4729-4732.1998","article-title":"Role of Fusobacterium nucleatum and coaggregation in anaerobe survival in planktonic biofilm oral microbial communities during aeration","volume":"66","author":"Bradshaw","year":"1998","journal-title":"Infect. Immun."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Turri, A., Cirgic, E., Shan, F., Hoffman, M., Omar, O., Dahlin, C., and Trobos, M. Early plaque formation on PTFE membranes with expanded or dense surface structures applied in the oral cavity of human volunteers. Clin. Exp. Dent. Res., 2020.","DOI":"10.1002\/cre2.344"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1111\/j.1600-0501.2006.01353.x","article-title":"Effect of material characteristics and\/or surface topography on biofilm development","volume":"17","author":"Teughels","year":"2006","journal-title":"Clin. Oral Implant. Res."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1046\/j.1365-2672.2001.01338.x","article-title":"The effects of surface roughness and type of denture acrylic on biofilm formation by Streptococcus oralis in a constant depth film fermentor","volume":"91","author":"Morgan","year":"2001","journal-title":"J. Appl. Microbiol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"2686","DOI":"10.1021\/la1046194","article-title":"Microtopographic patterns affect Escherichia coli biofilm formation on poly(dimethylsiloxane) surfaces","volume":"27","author":"Hou","year":"2011","journal-title":"Langmuir."},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Kunrath, M., and Dahlin, C. (2022). The Impact of Early Saliva Interaction on Dental Implants and Biomaterials for Oral Regeneration: An Overview. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23042024"}],"container-title":["Materials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1996-1944\/15\/16\/5705\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:11:52Z","timestamp":1760141512000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1996-1944\/15\/16\/5705"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8,18]]},"references-count":57,"journal-issue":{"issue":"16","published-online":{"date-parts":[[2022,8]]}},"alternative-id":["ma15165705"],"URL":"https:\/\/doi.org\/10.3390\/ma15165705","relation":{},"ISSN":["1996-1944"],"issn-type":[{"value":"1996-1944","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,8,18]]}}}