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To improve the treatment and shorten the healing time and, therefore, the cost, numerous types of wound dressings have been developed by manufacturers. Considering certain inconveniences related to the intolerance of some patients to antibiotics and the antimicrobial, antioxidant, and curative properties of certain essential oils, we conducted research by incorporating these oils, based on polyvinyl alcohol\/ polyvinyl pyrrolidone (PVA\/PVP) biopolymers, into dressings. The objective of this study was to study the potential of a polymeric matrix for wound healing, with polyvinyl alcohol as the main material and polyvinyl pyrrolidone and hydroxypropyl methylcellulose (HPMC) as secondary materials, together with additives (plasticizers poly(ethylene glycol) (PEG) and glycerol), stabilizers (Zn stearate), antioxidants (vitamin A and vitamin E), and four types of essential oils (fennel, peppermint, pine, and thyme essential oils). For all the studied samples, the combining compatibility, antimicrobial, and cytotoxicity properties were investigated. The obtained results demonstrated a uniform morphology for almost all the samples and adequate barrier properties for contact with suppurating wounds. The results show that the obtained samples containing essential oils have a good inhibitory effect on, or antimicrobial properties against, Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, and Candida albicans ATCC 10231. The MTT assay showed that the tested samples were not toxic and did not lead to cell death. The results showed that the essential oils used provide an effective solution as active substances in wound dressings.<\/jats:p>","DOI":"10.3390\/ma15196923","type":"journal-article","created":{"date-parts":[[2022,10,9]],"date-time":"2022-10-09T01:43:11Z","timestamp":1665279791000},"page":"6923","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":47,"title":["Essential Oils as Antimicrobial Active Substances in Wound Dressings"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3660-8926","authenticated-orcid":false,"given":"Daniela","family":"Gheorghita","sequence":"first","affiliation":[{"name":"Faculty of Material Science and Engineering, University Politehnica of Bucharest, 313 Splaiul Independentei Street, 060042 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Grosu","sequence":"additional","affiliation":[{"name":"Faculty of Material Science and Engineering, University Politehnica of Bucharest, 313 Splaiul Independentei Street, 060042 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9433-6725","authenticated-orcid":false,"given":"Alina","family":"Robu","sequence":"additional","affiliation":[{"name":"Faculty of Material Science and Engineering, University Politehnica of Bucharest, 313 Splaiul Independentei Street, 060042 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6593-255X","authenticated-orcid":false,"given":"Lia","family":"Ditu","sequence":"additional","affiliation":[{"name":"Faculty of Biology, University of Bucharest, 1-3 Intr. Portocalelor Street, 060101 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0597-0789","authenticated-orcid":false,"given":"Iuliana","family":"Deleanu","sequence":"additional","affiliation":[{"name":"Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 1-7 Polizu Street, 011061 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gratiela","family":"Gradisteanu Pircalabioru","sequence":"additional","affiliation":[{"name":"Research Institute of the University of Bucharest, 90 Sos. Panduri, 050663 Bucharest, Romania"},{"name":"Academy of Romanian Scientists, 54 Splaiul Independentei Street, 050094 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anca-Daniela","family":"Raiciu","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Titu Maiorescu University, 22 Dambovnicului Street, 040441 Bucharest, Romania"},{"name":"S.C. Hofigal Import Export S.A., 2 Intrarea Serelor Street, 042124 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1421-2018","authenticated-orcid":false,"given":"Ana-Iulia","family":"Bita","sequence":"additional","affiliation":[{"name":"Faculty of Material Science and Engineering, University Politehnica of Bucharest, 313 Splaiul Independentei Street, 060042 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aurora","family":"Antoniac","sequence":"additional","affiliation":[{"name":"Faculty of Material Science and Engineering, University Politehnica of Bucharest, 313 Splaiul Independentei Street, 060042 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0112-3494","authenticated-orcid":false,"given":"Vasile","family":"Antoniac","sequence":"additional","affiliation":[{"name":"Faculty of Material Science and Engineering, University Politehnica of Bucharest, 313 Splaiul Independentei Street, 060042 Bucharest, Romania"},{"name":"Academy of Romanian Scientists, 54 Splaiul Independentei Street, 050094 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e6687","DOI":"10.1097\/MD.0000000000006687","article-title":"Adverse Local Tissue Reaction after 2 Revision Hip Replacements for Ceramic Liner Fracture","volume":"96","author":"Antoniac","year":"2017","journal-title":"Medicine"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2200803","DOI":"10.1002\/admi.202200803","article-title":"Strontium Substituted Tricalcium Phosphate Bone Cement: Short and Long-Term Time-Resolved Studies and In Vitro Properties","volume":"9","author":"Rau","year":"2022","journal-title":"Adv. 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