{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T03:14:50Z","timestamp":1773717290077,"version":"3.50.1"},"reference-count":54,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2023,4,26]],"date-time":"2023-04-26T00:00:00Z","timestamp":1682467200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Banco Nacional de Desenvolvimento Econ\u00f4mico e Social (Brasil)","award":["PEQ-19391"],"award-info":[{"award-number":["PEQ-19391"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Materials"],"abstract":"<jats:p>Approximately 1 billion people are affected by neglected diseases around the world. Among these diseases, schistosomiasis constitutes one of the most important public health problems, being caused by Schistosoma mansoni and treated through the oral administration of praziquantel (PZQ). Despite being a common disease in children, the medication is delivered in the form of large, bitter-tasting tablets, which makes it difficult for patients to comply with the treatment. In order to mask the taste of the drug, allow more appropriate doses for children, and enhance the absorption by the body, different polymer matrices based on poly(methyl methacrylate) (PMMA) were developed and used to encapsulate PZQ. Polymer matrices included PMMA nano- and microparticles, PMMA-co-DEAEMA (2-(diethylamino)ethyl methacrylate), and PMMA-co-DMAEMA (2-(dimethylamino)ethyl methacrylate) microparticles. The performances of the drug-loaded particles were characterized in vitro through dissolution tests and in vivo through pharmacokinetic analyses in rats for the first time. The in vitro dissolution studies were carried out in accordance with the Brazilian Pharmacopeia and revealed a good PZQ release profile in an acidic medium for the PMMA-DEAEMA copolymer, reaching values close to 100 % in less than 3 h. The in vivo pharmacokinetic analyses were conducted using free PZQ as the control group that was compared with the investigated matrices. The drug was administered orally at doses of 60 mg\/kg, and the PMMA-co-DEAEMA copolymer microparticles were found to be the most efficient release system among the investigated ones, reaching a Cmax value of 1007 \u00b1 83 ng\/mL, even higher than that observed for free PZQ, which displayed a Cmax value of 432 \u00b1 98 ng\/mL.<\/jats:p>","DOI":"10.3390\/ma16093382","type":"journal-article","created":{"date-parts":[[2023,4,26]],"date-time":"2023-04-26T02:30:52Z","timestamp":1682476252000},"page":"3382","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["In Vitro Release and In Vivo Pharmacokinetics of Praziquantel Loaded in Different Polymer Particles"],"prefix":"10.3390","volume":"16","author":[{"given":"Emiliane Daher","family":"Pereira","sequence":"first","affiliation":[{"name":"Programa de Engenharia Qu\u00edmica\/COPPE, Universidade Federal do Rio de Janeiro, Cidade Universit\u00e1ria, CP: 68502, Rio de Janeiro 21941-972, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4693-6512","authenticated-orcid":false,"given":"Luciana","family":"da Silva Dutra","sequence":"additional","affiliation":[{"name":"Programa de Engenharia Qu\u00edmica\/COPPE, Universidade Federal do Rio de Janeiro, Cidade Universit\u00e1ria, CP: 68502, Rio de Janeiro 21941-972, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thamiris Franckini","family":"Paiva","sequence":"additional","affiliation":[{"name":"SENAI CETIQT, Instituto SENAI de Inova\u00e7\u00e3o em Biossint\u00e9ticos e Fibras, Cidade Universit\u00e1ria, Rua Fernando de Souza Barros, Rio de Janeiro 21941-857, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Larissa Leite","family":"de Almeida Carvalho","sequence":"additional","affiliation":[{"name":"Programa de Engenharia de Processos Qu\u00edmicos e Bioqu\u00edmicos\/EQ, Universidade Federal do Rio de Janeiro, Cidade Universit\u00e1ria, Rio de Janeiro 21949-900, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helv\u00e9cio Vin\u00edcius Antunes","family":"Rocha","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Micro e Nanotecnologia, Instituto de Tecnologia de F\u00e1rmacos\u2014Farmanguinhos, Funda\u00e7\u00e3o Oswaldo Cruz, Rio de Janeiro 21040-361, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2631-1811","authenticated-orcid":false,"given":"Jos\u00e9 Carlos","family":"Pinto","sequence":"additional","affiliation":[{"name":"Programa de Engenharia Qu\u00edmica\/COPPE, Universidade Federal do Rio de Janeiro, Cidade Universit\u00e1ria, CP: 68502, Rio de Janeiro 21941-972, RJ, Brazil"},{"name":"Programa de Engenharia de Processos Qu\u00edmicos e Bioqu\u00edmicos\/EQ, Universidade Federal do Rio de Janeiro, Cidade Universit\u00e1ria, Rio de Janeiro 21949-900, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1038\/449157a","article-title":"Neglected Diseases","volume":"449","year":"2007","journal-title":"Nature"},{"key":"ref_2","unstructured":"(2023, February 02). WHO Schistosomiasis. Available online: https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/schistosomiasis."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1016\/j.ijpara.2003.12.003","article-title":"Sex- and Stage-Related Sensitivity of Schistosoma Mansoni to in Vivo and in Vitro Praziquantel Treatment","volume":"34","author":"Cioli","year":"2004","journal-title":"Int. J. Parasitol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/j.actatropica.2017.08.002","article-title":"Schistosomiasis: Drugs Used and Treatment Strategies","volume":"176","author":"Fontes","year":"2017","journal-title":"Acta Trop."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"983","DOI":"10.1016\/j.ijpharm.2016.08.009","article-title":"Unexpected Solvent Impact in the Crystallinity of Praziquantel\/Poly(Vinylpyrrolidone) Formulations. A Solubility, DSC and Solid-State NMR Study","volume":"511","author":"Costa","year":"2016","journal-title":"Int. J. Pharm."},{"key":"ref_6","unstructured":"M\u00fcnster, M. (2018). Paediatric Drug Development for Praziquantel, Heinrich-Heine-Universit\u00e4t D\u00fcsseldorf."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1080\/17425247.2022.2051477","article-title":"Recent Trends in Praziquantel Nanoformulations for Helminthiasis Treatment","volume":"19","author":"Mengarda","year":"2022","journal-title":"Expert. Opin. Drug. Deliv."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/j.jconrel.2017.06.002","article-title":"Mechanical Microencapsulation: The Best Technique in Taste Masking for the Manufacturing Scale\u2014Effect of Polymer Encapsulation on Drug Targeting","volume":"260","author":"Alsirawan","year":"2017","journal-title":"J. Control. Release"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Gangapurwala, G., Vollrath, A., De San Luis, A., and Schubert, U.S. (2020). PLA\/PLGA-Based Drug Delivery Systems Produced with Supercritical CO2\u2014A Green Future for Particle Formulation?. Pharmaceutics, 12.","DOI":"10.3390\/pharmaceutics12111118"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Soh, S.H., and Lee, L.Y. (2019). Microencapsulation and Nanoencapsulation Using Supercritical Fluid (SCF) Techniques. Pharmaceutics, 11.","DOI":"10.3390\/pharmaceutics11010021"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"48039","DOI":"10.1002\/app.48039","article-title":"Material Encapsulation in Poly(Methyl Methacrylate) Shell: A Review","volume":"136","author":"Ahangaran","year":"2019","journal-title":"J. Appl. Polym. Sci."},{"key":"ref_12","first-page":"69","article-title":"Polymers in Drug Delivery","volume":"4","author":"Srivastava","year":"2016","journal-title":"J. Biosci. Med."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/j.matchemphys.2009.03.019","article-title":"Effect of Hydrophilic\u2013Hydrophobic Balance on Biocompatibility of Poly(Methyl Methacrylate) (PMMA)\u2013Hydroxyapatite (HA) Composites","volume":"118","author":"Tihan","year":"2009","journal-title":"Mater. Chem. Phys."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Awad, R., Avital, A., and Sosnik, A. (2022). Polymeric Nanocarriers for Nose-to-Brain Drug Delivery in Neurodegenerative Diseases and Neurodevelopmental Disorders. Acta Pharm. Sin. B, in press.","DOI":"10.1016\/j.apsb.2022.07.003"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"111379","DOI":"10.1016\/j.eurpolymj.2022.111379","article-title":"Research Progress of Poly(Methyl Methacrylate) Microspheres: Preparation, Functionalization and Application","volume":"175","author":"Gao","year":"2022","journal-title":"Eur. Polym. J."},{"key":"ref_16","first-page":"97","article-title":"PMMA-Based Biomedical Applications: Manufacture and Uses","volume":"Volume 141","author":"Castor","year":"2019","journal-title":"Advances in Medicine and Biology"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"353","DOI":"10.3109\/02652048.2011.651500","article-title":"Poly(Methyl Methacrylate) Particulate Carriers in Drug Delivery","volume":"29","author":"Bettencourt","year":"2012","journal-title":"J. Microencapsul."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1002\/mren.201200036","article-title":"Production of PMMA Nanoparticles Loaded with Praziquantel Through \u201cIn Situ\u201d Miniemulsion Polymerization","volume":"7","author":"Fonseca","year":"2013","journal-title":"Macromol. React. Eng."},{"key":"ref_19","first-page":"85","article-title":"Desenvolvimento de Um Medicamento Brasileiro Nanoencapsulado Para o Tratamento Da Esquistossomose","volume":"1","author":"Mattos","year":"2013","journal-title":"Vigil\u00e2ncia Sanit\u00e1ria Em Debate Soc. Ci\u00eanc. Tecnol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1800064","DOI":"10.1002\/mren.201800064","article-title":"In Situ Incorporation of Praziquantel in Polymer Microparticles through Suspension Polymerization for Treatment of Schistosomiasis","volume":"13","author":"Paiva","year":"2018","journal-title":"Macromol. React. Eng."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1900028","DOI":"10.1002\/mren.201900028","article-title":"Development of Smart Polymer Microparticles through Suspension Polymerization for Treatment of Schistosomiasis","volume":"13","author":"Paiva","year":"2019","journal-title":"Macromol. React. Eng."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1002\/pls2.10037","article-title":"In Situ Encapsulation of Praziquantel through Methyl Methacrylate\/Diethylaminoethyl Methacrylate and MMA\/DMAEMA Miniemulsion Copolymerizations in Presence of Distinct Ionic Surfactants","volume":"2","author":"Alves","year":"2021","journal-title":"SPE Polym."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"100068","DOI":"10.1016\/j.apsadv.2021.100068","article-title":"Towards a New Class of Stimuli-Responsive Polymer-Based Materials\u2014Recent Advances and Challenges","volume":"4","year":"2021","journal-title":"Appl. Surf. Sci. Adv."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.colsurfb.2008.01.001","article-title":"Synthesis and Properties of PH and Temperature Sensitive P(NIPAAm-Co-DMAEMA) Hydrogels","volume":"64","author":"Wang","year":"2008","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.msec.2018.06.020","article-title":"Fabrication and Characterization of Chicken Feather Keratin\/Polysaccharides Blended Polymer Coated Nonwoven Dressing Materials for Wound Healing Applications","volume":"92","author":"Shanmugasundaram","year":"2018","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"281","DOI":"10.4103\/0253-7613.27026","article-title":"Effect of Acute and Chronic Treatment of Losartan Potassium on Tail-Flick Response in Mice","volume":"38","author":"Nagappa","year":"2006","journal-title":"Indian J. Pharmacol."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Bardal, S.K., Waechter, J.E., and Martin, D.S. (2011). Applied Pharmacology, Elsevier.","DOI":"10.1016\/B978-1-4377-0310-8.00013-0"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Di, L., and Kerns, E.H. (2016). Drug-Like Properties, Elsevier.","DOI":"10.1016\/B978-0-12-801076-1.00020-4"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1274","DOI":"10.1016\/j.addr.2006.09.007","article-title":"Mathematical Modeling and Simulation of Drug Release from Microspheres: Implications to Drug Delivery Systems","volume":"58","author":"Arifin","year":"2006","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Lin, Y.-S., and Tsay, R.-Y. (2020). Drug Release from a Spherical Matrix: Theoretical Analysis for a Finite Dissolution Rate Affected by Geometric Shape of Dispersed Drugs. Pharmaceutics, 12.","DOI":"10.3390\/pharmaceutics12060582"},{"key":"ref_31","unstructured":"ANVISA (2010). Farmacop\u00e9ia Brasileira."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1208\/s12248-010-9185-1","article-title":"DDSolver: An Add-In Program for Modeling and Comparison of Drug Dissolution Profiles","volume":"12","author":"Zhang","year":"2010","journal-title":"AAPS J."},{"key":"ref_33","unstructured":"Bruschi, M.L. (2015). Strategies to Modify the Drug Release from Pharmaceutical Systems, Woodhead Publishing."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1637","DOI":"10.1016\/0009-2509(96)00024-3","article-title":"Polymerization Reactor Modeling: A Review of Recent Developments and Future Directions","volume":"51","author":"Kiparissides","year":"1996","journal-title":"Chem. Eng. Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0168-3659(00)00339-4","article-title":"Biodegradable Polymeric Nanoparticles as Drug Delivery Devices","volume":"70","author":"Soppimath","year":"2001","journal-title":"J. Control. Release"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2512","DOI":"10.1007\/s11095-012-0958-3","article-title":"Effects of Particle Size and Surface Modification on Cellular Uptake and Biodistribution of Polymeric Nanoparticles for Drug Delivery","volume":"30","author":"Kulkarni","year":"2013","journal-title":"Pharm. Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1737","DOI":"10.1002\/ceat.201000210","article-title":"Suspension Polymerization Processes","volume":"33","author":"Brooks","year":"2010","journal-title":"Chem. Eng. Technol."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Danaei, M., Dehghankhold, M., Ataei, S., Hasanzadeh Davarani, F., Javanmard, R., Dokhani, A., Khorasani, S., and Mozafari, M. (2018). Impact of Particle Size and Polydispersity Index on the Clinical Applications of Lipidic Nanocarrier Systems. Pharmaceutics, 10.","DOI":"10.3390\/pharmaceutics10020057"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1021\/acs.molpharmaceut.6b00896","article-title":"Effect of Particle Size on Drug Loading and Release Kinetics of Gefitinib-Loaded PLGA Microspheres","volume":"14","author":"Chen","year":"2017","journal-title":"Mol. Pharm."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1002\/masy.201600028","article-title":"Comparative Analysis of Sunscreen Nanoencapsulation Processes","volume":"368","author":"Paiva","year":"2016","journal-title":"Macromol. Symp."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"5609","DOI":"10.1021\/la5007583","article-title":"Insight in the Phase Separation Peculiarities of Poly(Dialkylaminoethyl Methacrylate)s","volume":"30","author":"Thavanesan","year":"2014","journal-title":"Langmuir"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"4391","DOI":"10.1039\/C5CS00873E","article-title":"CO2-Responsive Polymeric Materials: Synthesis, Self-Assembly, and Functional Applications","volume":"45","author":"Darabi","year":"2016","journal-title":"Chem. Soc. Rev."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1002\/pi.4335","article-title":"Synthesis, Characterization and Thermal Degradation of Dual Temperature- and PH-Sensitive RAFT-Made Copolymers of N,N-(Dimethylamino)Ethyl Methacrylate and Methyl Methacrylate","volume":"62","author":"Roy","year":"2013","journal-title":"Polym. Int."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"953","DOI":"10.1016\/j.cej.2017.06.170","article-title":"Drug Release of Yolk\/Shell Microcapsule Controlled by PH-Responsive Yolk Swelling","volume":"327","author":"Jia","year":"2017","journal-title":"Chem. Eng. J."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1021\/acs.macromol.5b02458","article-title":"A New Thermo-, PH-, and CO2-Responsive Homopolymer of Poly[N-[2-(Diethylamino)Ethyl]Acrylamide]: Is the Diethylamino Group Underestimated?","volume":"49","author":"Song","year":"2016","journal-title":"Macromolecules"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"012063","DOI":"10.1088\/1742-6596\/1295\/1\/012063","article-title":"Modelling of Controlled Drug Release in Gastrointestinal Tract Simulation","volume":"1295","author":"Permanadewi","year":"2019","journal-title":"J. Phys. Conf. Ser."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"111081","DOI":"10.1016\/j.matdes.2022.111081","article-title":"Self-Expanding PMMA Composite Bone Cement with Sustained Release of Gentamicin Sulfate and Alendronate Using Water Absorption Pathways","volume":"222","author":"Chen","year":"2022","journal-title":"Mater. Des."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.jcou.2019.08.025","article-title":"Production and Computational Fluid Dynamics-Based Modeling of PMMA Nanoparticles Impregnated with Ivermectin by a Supercritical Antisolvent Process","volume":"35","year":"2020","journal-title":"J. CO2 Util."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"7112","DOI":"10.1007\/s10853-018-2071-4","article-title":"Antibiotic and Anesthetic Drug Release from Double-Setting \u03b1-TCP Cements","volume":"53","author":"Colpo","year":"2018","journal-title":"J. Mater. Sci."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1146\/annurev-food-030216-025720","article-title":"In Vitro Release Kinetics of Microencapsulated Materials and the Effect of the Food Matrix","volume":"8","author":"Flores","year":"2017","journal-title":"Annu. Rev. Food Sci. Technol."},{"key":"ref_51","first-page":"201","article-title":"No\u00e7\u00f5es Sobre Par\u00e2metros Farmacocin\u00e9ticos\/Farmacodin\u00e2micos e Sua Utiliza\u00e7\u00e3o Na Pr\u00e1tica M\u00e9dica 2017","volume":"15","author":"Federico","year":"2017","journal-title":"Rev. Soc. Bras. Cl\u00ednica M\u00e9dica"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"674","DOI":"10.1023\/A:1018968011169","article-title":"Mucoadhesion of Latexes. I. Analytical Methods and Kinetic Studies","volume":"11","author":"Durrer","year":"1994","journal-title":"Pharm. Res."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1568","DOI":"10.1023\/A:1012126301290","article-title":"The Mechanism of Uptake of Biodegradable Microparticles in Caco-2 Cells Is Size Dependent","volume":"14","author":"Desai","year":"1997","journal-title":"Pharm. Res."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"65","DOI":"10.3390\/10010065","article-title":"Polymeric Particulates to Improve Oral Bioavailability of Peptide Drugs","volume":"10","author":"Delie","year":"2005","journal-title":"Molecules"}],"container-title":["Materials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1996-1944\/16\/9\/3382\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:23:42Z","timestamp":1760124222000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1996-1944\/16\/9\/3382"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4,26]]},"references-count":54,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2023,5]]}},"alternative-id":["ma16093382"],"URL":"https:\/\/doi.org\/10.3390\/ma16093382","relation":{},"ISSN":["1996-1944"],"issn-type":[{"value":"1996-1944","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,4,26]]}}}