{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T16:47:42Z","timestamp":1781196462242,"version":"3.54.1"},"reference-count":41,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2024,6,11]],"date-time":"2024-06-11T00:00:00Z","timestamp":1718064000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Large-scale Scientific Instruments Open and Shared Independent Research Project of Jiangsu Province","award":["TC2023A042"],"award-info":[{"award-number":["TC2023A042"]}]},{"name":"Large-scale Scientific Instruments Open and Shared Independent Research Project of Jiangsu Province","award":["51403146"],"award-info":[{"award-number":["51403146"]}]},{"name":"Large-scale Scientific Instruments Open and Shared Independent Research Project of Jiangsu Province","award":["BK20161288"],"award-info":[{"award-number":["BK20161288"]}]},{"name":"National Natural Science Foundation of China","award":["TC2023A042"],"award-info":[{"award-number":["TC2023A042"]}]},{"name":"National Natural Science Foundation of China","award":["51403146"],"award-info":[{"award-number":["51403146"]}]},{"name":"National Natural Science Foundation of China","award":["BK20161288"],"award-info":[{"award-number":["BK20161288"]}]},{"name":"Natural Science Foundation of Jiangsu Province","award":["TC2023A042"],"award-info":[{"award-number":["TC2023A042"]}]},{"name":"Natural Science Foundation of Jiangsu Province","award":["51403146"],"award-info":[{"award-number":["51403146"]}]},{"name":"Natural Science Foundation of Jiangsu Province","award":["BK20161288"],"award-info":[{"award-number":["BK20161288"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Materials"],"abstract":"<jats:p>Appropriate biodegradability to meet the demands of wound repair is critical for superficial wound repair membrane applications. Tyrosinase-catalyzed crosslinking SF (c-SF) membranes were constructed and regulated the degradation behavior in this study. The crosslinking degree of the c-SF membranes could be adjusted by reaction ratios of tyrosinase against SF (TYR\/SF). Upon reaching a TYR\/SF ratio of 20\/6000, the degree of crosslinking increased to 88.17 \u00b1 0.20%, without obvious changes in the crystal structure. The degradation behavior was regulated by the TYR\/SF ratio and the degradation environment. All c-SF membranes remained stable after immersion without collagenase but showed an adjustable degradation behavior in the presence of collagenase. As the TYR\/SF ratio increased, the residual weights increased from 23.31 \u00b1 1.35% to 60.12 \u00b1 0.82% after 7 days of degradation, occurring with low increased amounts of \u03b2-sheet structure and free amino acids. This work provides a new c-SF membrane with controllable rapid degradability and favorable cytocompatibility, which can help to meet requirements for biodegradable superficial wound repair membranes.<\/jats:p>","DOI":"10.3390\/ma17122839","type":"journal-article","created":{"date-parts":[[2024,6,11]],"date-time":"2024-06-11T11:58:58Z","timestamp":1718107138000},"page":"2839","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Regulation of the Degradation Properties of Tyrosinase-Catalyzed Crosslinking Silk Membranes for Superficial Wound Repair"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8025-1488","authenticated-orcid":false,"given":"Yu","family":"Liu","sequence":"first","affiliation":[{"name":"National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou 215123, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuping","family":"Liu","sequence":"additional","affiliation":[{"name":"National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou 215123, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhong","family":"Jiao","sequence":"additional","affiliation":[{"name":"National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou 215123, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3353-9868","authenticated-orcid":false,"given":"Mingzhong","family":"Li","sequence":"additional","affiliation":[{"name":"National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou 215123, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,6,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"22786","DOI":"10.1021\/acsomega.0c01750","article-title":"Characterization of ground silk fibroin through comparison of nanofibroin and higher order structures","volume":"5","author":"Narita","year":"2020","journal-title":"ACS Omega"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"De Giorgio, G., Matera, B., Vurro, D., Manfredi, E., Galstyan, V., Tarabella, G., Ghezzi, B., and D\u2019Angelo, P. (2024). Silk fibroin materials: Biomedical applications and perspectives. Bioengineering, 11.","DOI":"10.3390\/bioengineering11020167"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Koczo\u0144, P., D\u0105browska, A., Laskowska, E., \u0141abuz, M., Maj, K., Masztakowski, J., Bartyzel, B.J., Brys, A., Brys, J., and Gruczy\u0144ska-S\u0119kowska, E. (2023). Applications of silk fibroin in human and veterinary medicine. Materials, 16.","DOI":"10.3390\/ma16227128"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Murchio, S., Benedetti, M., Berto, A., Agostinacchio, F., Zappini, G., and Maniglio, D. (2022). Hybrid Ti6Al4V\/silk fibroin composite for load-bearing implants: A hierarchical multifunctional cellular scaffold. Materials, 15.","DOI":"10.3390\/ma15176156"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"4868","DOI":"10.1016\/j.csbj.2023.10.012","article-title":"A comprehensive review of silk-fibroin hydrogels for cell and drug delivery applications in tissue engineering and regenerative medicine","volume":"21","author":"Madappura","year":"2023","journal-title":"Comput. Struct Biotechnol. J."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Mu, X., Sahoo, J.K., Cebe, P., and Kaplan, D.L. (2020). Photo-crosslinked silk fibroin for 3D printing. Polymers, 12.","DOI":"10.3390\/polym12122936"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Liu, J., Li, T., Zhang, H., Zhao, W.W., Qu, L.J., Chen, S.J., and Wu, S.H. (2022). Electrospun strong, bioactive, and bioabsorbable silk fibroin\/poly (L-lactic-acid) nanoyarns for constructing advanced nanotextile tissue scaffolds. Mater. Today Bio, 14.","DOI":"10.1016\/j.mtbio.2022.100243"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"979","DOI":"10.1007\/s42765-022-00229-5","article-title":"Living electrospun short fibrous sponge via engineered nanofat for wound healing","volume":"5","author":"Fu","year":"2022","journal-title":"Adv. Fiber Mater."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Liu, J., Zhang, J., Liu, J., Sun, W., Li, W., Shen, H., Wang, L., and Li, G. (2023). Advances in fiber-based wearable sensors for personal digital health monitoring. Materials, 16.","DOI":"10.3390\/ma16237428"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Sideratou, Z., Biagiotti, M., Tsiourvas, D., Panagiotaki, K.N., Zucca, M.V., Freddi, G., Lovati, A.B., and Bottagisio, M. (2022). Antibiotic-loaded hyperbranched polyester embedded into peptide-enriched silk fibroin for the treatment of orthopedic or dental infections. Nanomaterials, 12.","DOI":"10.3390\/nano12183182"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Tan, G., Jia, T., Qi, Z., and Lu, S. (2024). Regenerated fiber\u2019s ideal target: Comparable to natural fiber. Materials, 17.","DOI":"10.3390\/ma17081834"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"561","DOI":"10.32604\/jrm.2022.017434","article-title":"A review of recent advances in hybrid natural fiber reinforced polymer composites","volume":"10","author":"Neto","year":"2022","journal-title":"J. Renew. Mater."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Metalssi, O., Ragoug, R., Barberon, F., De Lacaillerie, J., Roussel, N., Divet, L., and Torrenti, J. (2023). Effect of an early-age exposure on the degradation mechanisms of cement paste under external sulfate attack. Materials, 16.","DOI":"10.3390\/ma16176013"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1588","DOI":"10.1016\/j.ijbiomac.2022.10.244","article-title":"Biodegradable film based on cress seed mucilage, modified with lutein, maltodextrin and alumina nanoparticles: Physicochemical properties and lutein controlled release","volume":"224","author":"Pirsa","year":"2023","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"119928","DOI":"10.1016\/j.jenvman.2023.119928","article-title":"Nanocellulose: Structure, modification, biodegradation and applications in agriculture as slow\/controlled release fertilizer, superabsorbent, and crop protection: A review","volume":"352","author":"Channab","year":"2024","journal-title":"J. Environ. Manag."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"133578","DOI":"10.1016\/j.foodchem.2022.133578","article-title":"Transglutaminase modified type a gelatin gel: The influence of intra-molecular and inter-molecular crosslinking on structure-properties","volume":"395","author":"Mao","year":"2022","journal-title":"Food Chem."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1907851","DOI":"10.1002\/adfm.201907851","article-title":"Reversible water transportation diode: Temperature-adaptive smart janus textile for moisture\/thermal management","volume":"30","author":"Wang","year":"2020","journal-title":"Adv. Funct. Mater."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"11088","DOI":"10.1021\/acssuschemeng.2c00949","article-title":"Degradation of regenerated silk fibroin in soil and marine environments","volume":"10","author":"Augustine","year":"2022","journal-title":"ACS Sustain. Chem. Eng."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Zhao, M., Qi, Z., Tao, X., Newkirk, C., Hu, X., and Lu, S. (2021). Chemical, thermal, time, and enzymatic stability of silk materials with silk i structure. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22084136"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"147144","DOI":"10.1016\/j.scitotenv.2021.147144","article-title":"Discharge of microplastics fibres from wet wipes in aquatic and solid environments under different release conditions","volume":"784","author":"Lee","year":"2021","journal-title":"Sci. Total Environ."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Chouhan, D., Dey, N., Bhardwaj, N., and Mandal, B.B. (2019). Emerging and innovative approaches for wound healing and skin regeneration: Current status and advances. Biomaterials, 216.","DOI":"10.1016\/j.biomaterials.2019.119267"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1007\/s42765-022-00216-w","article-title":"Electrospun aligned PCL\/gelatin scaffolds mimicking the skin ECM for effective antimicrobial wound dressings","volume":"5","author":"Ghomi","year":"2022","journal-title":"Adv. Fiber Mater."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Kim, H.S., Kumbar, S.G., and Nukavarapu, S.P. (2021). Biomaterial-directed cell behavior for tissue engineering. Curr. Opin. Biomed. Eng., 17.","DOI":"10.1016\/j.cobme.2020.100260"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"5496","DOI":"10.1021\/acsomega.3c07174","article-title":"Development of mupirocin-impregnated bacterial cellulosic transdermal patches for the management of skin infection","volume":"9","author":"Bhat","year":"2024","journal-title":"ACS Omega"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1177\/08853282231191218","article-title":"An alginate-based tube gel delivering 2-deoxy-D-ribose for stimulation of wound healing","volume":"38","author":"Abid","year":"2023","journal-title":"J. Biomater. Appl."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1176","DOI":"10.1093\/jbcr\/irad015","article-title":"The use of a polylactic membrane in pediatric burns proves to be successful even after late application","volume":"7","author":"Fernandes","year":"2023","journal-title":"J. Burn. Care Res."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Ouyang, J., Hu, N., and Wang, H. (2024). Isolation, purification and tyrosinase inhibitory activity of anthocyanins and their novel degradation compounds from solanum tuberosum. Molecules, 29.","DOI":"10.3390\/molecules29071492"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1016\/j.freeradbiomed.2021.05.040","article-title":"Antioxidant properties of anthocyanins and their mechanism of action in atherosclerosis","volume":"172","author":"Garcia","year":"2021","journal-title":"Free Radic. Biol. Med."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Filip, N., Radu, I., Veliceasa, B., Filip, C., Pertea, M., Clim, A., Pinzariu, A.C., Drochioi, I.C., Hilitanu, R.L., and Serban, I.L. (2022). Biomaterials in orthopedic devices: Current issues and future perspectives. Coatings, 12.","DOI":"10.3390\/coatings12101544"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1007\/s10409-021-01116-9","article-title":"Biomechanical study on implantable and interventional medical devices","volume":"37","author":"Wang","year":"2021","journal-title":"Acta Mech. Sin."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Kim, S.H., Kim, K., Kim, B.S., An, Y.H., Lee, U.J., Lee, S.H., Kim, S.L., Kim, B.G., and Hwang, N.S. (2020). Fabrication of polyphenol-incorporated anti-inflammatory hydrogel via high-affinity enzymatic crosslinking for wet tissue adhesion. Biomaterials, 26.","DOI":"10.1016\/j.biomaterials.2020.119905"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"127948","DOI":"10.1016\/j.foodchem.2020.127948","article-title":"Tyrosinase\/caffeic acid cross-linking alleviated shrimp (Metapenaeus ensis) tropomyosin-induced allergic responses by modulating the Th1\/Th2 immunobalance","volume":"15","author":"Ahmed","year":"2021","journal-title":"Food Chem."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Bounegru, A.V., and Apetrei, C. (2023). Tyrosinase immobilization strategies for the development of electrochemical biosensors-a review. Nanomaterials, 17.","DOI":"10.3390\/nano13040760"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Wang, M.M., Wang, Y., Pan, P., Liu, X.P., Zhang, W.J., Hu, C., and Li, M.Z. (2023). A high molecular weight silk fibroin scaffold that resists degradation and promotes cell proliferation. Biopolymers, 114.","DOI":"10.1002\/bip.23554"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1080\/10826068.2015.1084637","article-title":"Microbial production of amino acid-modified spider dragline silk protein with intensively improved mechanical properties","volume":"46","author":"Zhang","year":"2016","journal-title":"Prep. Biochem. Biotechnol."},{"key":"ref_36","unstructured":"(2017). Standard Test Method for Tensile Properties of Polymer Matrix Composite Materials (Standard No. ASTM D3039\/D3039M-00)."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1038\/s41594-024-01211-y","article-title":"A novel class of inhibitors that disrupts the stability of integrin heterodimers identified by CRISPR-tiling-instructed genetic screens","volume":"31","author":"Mattson","year":"2024","journal-title":"Nat. Struct. Mol. Biol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s12221-021-3276-8","article-title":"Biocompatible gradient chitosan fibers with controllable swelling and antibacterial properties","volume":"23","author":"Liu","year":"2022","journal-title":"Fibers Polym."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Janowicz, M., Galus, S., Szulc, K., Ciurzynska, A., and Nowacka, M. (2024). Investigation of the structure forming potential of protein components in the reformulation of the composition of edible films. Materials, 17.","DOI":"10.3390\/ma17040937"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1177\/0885328220928660","article-title":"Silk fibroin-based vascular repairing sheet with angiogenic-promoting activity of SVVYGLR peptide regenerated the damaged vascular in rats","volume":"37","author":"Shimada","year":"2022","journal-title":"J. Biomater. Appl."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"3359","DOI":"10.1038\/s41467-020-17034-6","article-title":"Subsurface chemical nanoidentification by nano-FTIR spectroscopy","volume":"11","author":"Mester","year":"2020","journal-title":"Nat. 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