{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T10:17:08Z","timestamp":1771237028101,"version":"3.50.1"},"reference-count":95,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T00:00:00Z","timestamp":1722988800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Minister of Science and Higher Education Republic of Poland within the program \u201cRegional Excellence Initiative\u201d","award":["RID\/SP\/0032\/2024\/01"],"award-info":[{"award-number":["RID\/SP\/0032\/2024\/01"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Materials"],"abstract":"<jats:p>One of the intensively developed tools for cancer therapy is drug-releasing matrices. Polyamidoamine dendrimers (PAMAM) are commonly used as nanoparticles to increase the solubility, stability and retention of drugs in the human body. Most often, drugs are encapsulated in PAMAM cavities or covalently attached to their surface. However, there are no data on the use of PAMAM dendrimers as a component of porous matrices based on polyurethane foams for the controlled release of drugs and biologically active substances. Therefore, in this work, porous materials based on polyurethane foam with incorporated third-generation poly(amidoamine) dendrimers (PAMAM G3) were synthesized and characterized. Density, water uptake and morphology of foams were examined with SEM and XPS. The PAMAM was liquefied with polyether polyol (G441) and reacted with polymeric 4,4\u2032-diphenylmethane diisocyanate (pMDI) in the presence of silicone, water and a catalyst to obtain foam (PF1). In selected compositions, the castor oil was added (PF2). Analogs without PAMAM G3 were also synthesized (F1 and F2, respectively). An SEM analysis of foams showed that they are composed of thin ribs\/walls forming an interconnected network containing hollow bubbles\/pores and showing some irregularities in the structure. Foam from a G3:G441:CO (PF2) composition is characterized by a more regular structure than the foam from the composition without castor oil. The encapsulation efficiency of drugs determined by the XPS method shows that it varies depending on the matrix and the drug and ranges from several to a dozen mass percent. In vitro biological studies with direct contact and extract assays indicated that the F2 matrix was highly biocompatible. Significant toxicity of dendrimeric matrices PF1 and PF2 containing 50% of PAMAM G3 was higher against human squamous carcinoma cells than human immortalized keratinocytes. The ability of the matrices to immobilize drugs was demonstrated in the example of perspective (Nimesulide, 8-Methoxypsolarene) or approved anticancer drugs (Doxorubicin\u2014DOX, 5-Aminolevulinic acid). Release into the culture medium and penetration of DOX into the tested SCC-15 and HaCaT cells were also proved. The results show that further modification of the obtained matrices may lead to their use as drug delivery systems, e.g., for anticancer therapy.<\/jats:p>","DOI":"10.3390\/ma17163905","type":"journal-article","created":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T11:33:52Z","timestamp":1723030432000},"page":"3905","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["A Novel PAMAM G3 Dendrimer-Based Foam with Polyether Polyol and Castor Oil Components as Drug Delivery System into Cancer and Normal Cells"],"prefix":"10.3390","volume":"17","author":[{"given":"Magdalena","family":"Zar\u0119ba","sequence":"first","affiliation":[{"name":"The Faculty of Chemistry, Rzeszow University of Technology, 35-959 Rzeszow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5000-2296","authenticated-orcid":false,"given":"El\u017cbieta","family":"Chmiel-Szukiewicz","sequence":"additional","affiliation":[{"name":"The Faculty of Chemistry, Rzeszow University of Technology, 35-959 Rzeszow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7964-0777","authenticated-orcid":false,"given":"\u0141ukasz","family":"Uram","sequence":"additional","affiliation":[{"name":"The Faculty of Chemistry, Rzeszow University of Technology, 35-959 Rzeszow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Justyna","family":"Noga","sequence":"additional","affiliation":[{"name":"The Faculty of Chemistry, Rzeszow University of Technology, 35-959 Rzeszow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0277-6382","authenticated-orcid":false,"given":"Magdalena","family":"Rzepna","sequence":"additional","affiliation":[{"name":"Centre for Radiation Research and Technology, Institute of Nuclear Chemistry and Technology, 03-195 Warsaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3215-9350","authenticated-orcid":false,"given":"Stanis\u0142aw","family":"Wo\u0142owiec","sequence":"additional","affiliation":[{"name":"Medical College, University of Rzeszow, 1a Warzywna Street, 35-310 Rzeszow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2719","DOI":"10.1002\/pola.10301","article-title":"Discovery of Dendrimers and Dendritic Polymers: A Brief Historical Perspective","volume":"40","author":"Tomalia","year":"2002","journal-title":"J. Polym. Sci. A Polym. Chem."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"3799","DOI":"10.1016\/S0040-4020(03)00430-7","article-title":"Structure Control within Poly(Amidoamine) Dendrimers: Size, Shape and Regio-Chemical Mimicry of Globular Proteins","volume":"59","author":"Tomalia","year":"2003","journal-title":"Tetrahedron"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"5956","DOI":"10.1021\/ja000378x","article-title":"Reactivity at the Interface of Chiral Amphiphilic Dendrimers. High Asymmetric Reduction by NaBH4 of Various Prochiral Ketones","volume":"123","author":"Schmitzer","year":"2001","journal-title":"J. Am. Chem. Soc."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/S0378-5173(02)00623-3","article-title":"The Influence of Surface Modification on the Cytotoxicity of PAMAM Dendrimers","volume":"252","author":"Jevprasesphant","year":"2003","journal-title":"Int. J. Pharm."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1016\/j.jconrel.2023.03.011","article-title":"Precise and Systematic End Group Chemistry Modifications on PAMAM and Poly(l-Lysine) Dendrimers to Improve Cytosolic Delivery of mRNA","volume":"356","author":"Joubert","year":"2023","journal-title":"J. Control. Release"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"25557","DOI":"10.1039\/D0RA02574G","article-title":"Efficient Modification of PAMAM G1 Dendrimer Surface with \u03b2-Cyclodextrin Units by CuAAC: Impact on the Water Solubility and Cytotoxicity","volume":"10","author":"Solano","year":"2020","journal-title":"RSC Adv."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.mtchem.2019.04.004","article-title":"Poly(Amidoamine) Dendrimers: Covalent and Supramolecular Synthesis","volume":"13","author":"Lyu","year":"2019","journal-title":"Mater. Today Chem."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1016\/S1359-6446(01)01757-3","article-title":"Poly(Amidoamine) (PAMAM) Dendrimers: From Biomimicry to Drug Delivery and Biomedical Applications","volume":"6","author":"Esfand","year":"2001","journal-title":"Drug Discov. Today"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1166\/asfo.2013.1040","article-title":"Dendrimers as Novel Polymeric Material: A Review on Its Synthesis, Characterization and Their Applications","volume":"1","author":"Nasibullah","year":"2013","journal-title":"Adv. Sci. Focus"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Chis, A.A., Dobrea, C., Morgovan, C., Arseniu, A.M., Rus, L.L., Butuca, A., Juncan, A.M., Totan, M., Vonica-Tincu, A.L., and Cormos, G. (2020). Applications and Limitations of Dendrimers in Biomedicine. Molecules, 25.","DOI":"10.3390\/molecules25173982"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"6009","DOI":"10.1016\/j.arabjc.2020.05.002","article-title":"Starburst Pamam Dendrimers: Synthetic Approaches, Surface Modifications, and Biomedical Applications","volume":"13","author":"Kharwade","year":"2020","journal-title":"Arab. J. Chem."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Vu, M.T., Bach, L.G., Nguyen, D.C., Ho, M.N., Nguyen, N.H., Tran, N.Q., Nguyen, D.H., Nguyen, C.K., and Hoang Thi, T.T. (2019). Modified Carboxyl-Terminated PAMAM Dendrimers as Great Cytocompatible Nano-Based Drug Delivery System. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20082016"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"120485","DOI":"10.1016\/j.ijpharm.2021.120485","article-title":"Dendrimers Based Cancer Nanotheranostics: An Overview","volume":"600","author":"Saluja","year":"2021","journal-title":"Int. J. Pharm."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Shadrack, D.M., Swai, H.S., Munissi, J.J.E., Mubofu, E.B., and Nyandoro, S.S. (2018). Polyamidoamine Dendrimers for Enhanced Solubility of Small Molecules and Other Desirable Properties for Site Specific Delivery: Insights from Experimental and Computational Studies. Molecules, 23.","DOI":"10.3390\/molecules23061419"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Choudhary, S., Gupta, L., Rani, S., Dave, K., and Gupta, U. (2017). Impact of Dendrimers on Solubility of Hydrophobic Drug Molecules. Front. Pharmacol., 8.","DOI":"10.3389\/fphar.2017.00261"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1007\/s12272-018-1008-4","article-title":"Recent Progress in Dendrimer-Based Nanomedicine Development","volume":"41","author":"Kim","year":"2018","journal-title":"Arch. Pharm. Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1589","DOI":"10.1039\/D2BM01677J","article-title":"Functionalized PAMAM Constructed Nanosystems for Biomacromolecule Delivery","volume":"11","author":"Wong","year":"2023","journal-title":"Biomater. Sci."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Surekha, B., Kommana, N.S., Dubey, S.K., Kumar, A.V.P., Shukla, R., and Kesharwani, P. (2021). PAMAM Dendrimer as a Talented Multifunctional Biomimetic Nanocarrier for Cancer Diagnosis and Therapy. Colloids Surf. B Biointerfaces, 204.","DOI":"10.1016\/j.colsurfb.2021.111837"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.apmt.2018.05.002","article-title":"PAMAM Dendrimers as Efficient Drug and Gene Delivery Nanosystems for Cancer Therapy","volume":"12","author":"Dehghan","year":"2018","journal-title":"Appl. Mater. Today"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.ijpharm.2018.05.045","article-title":"Poly (Amidoamine) (PAMAM) Dendrimer Mediated Delivery of Drug and pDNA\/siRNA for Cancer Therapy","volume":"546","author":"Li","year":"2018","journal-title":"Int. J. Pharm."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.colsurfb.2017.04.003","article-title":"PAMAM (Generation 4) Incorporated Gelatin 3D Matrix as an Improved Dermal Substitute for Skin Tissue Engineering","volume":"155","author":"Maji","year":"2017","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"ref_22","first-page":"140","article-title":"Fabrication and Characterization of Matrix Metalloproteinase-Responsive G4 PAMAM-IBU\/GelMA Hydrogel","volume":"41","author":"Cai","year":"2021","journal-title":"J. Shanghai Jiaotong Univ. (Med. Sci.)"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1286","DOI":"10.1166\/jbn.2013.1534","article-title":"PAMAM Dendrimer Generation 5\u2013Pluronic F127 Nanofilm as a Matrix for Local Metronidazole Release","volume":"9","author":"Dung","year":"2013","journal-title":"J. Biomed. Nanotechnol."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Shifeta, N.T., Hamukwaya, S.L., An, Q., Hao, H., and Mashingaidze, M.M. (2022). Layer-by-Layer Fabrication of PAH\/PAMAM\/Nano-CaCO3 Composite Films and Characterization for Enhanced Biocompatibility. Int. J. Biomater., 2022.","DOI":"10.1155\/2022\/6331465"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Maimouni, I., Cejas, C.M., Cossy, J., Tabeling, P., and Russo, M. (2020). Microfluidics Mediated Production of Foams for Biomedical Applications. Micromachines, 11.","DOI":"10.3390\/mi11010083"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.ejpb.2021.07.012","article-title":"Foams as Unique Drug Delivery Systems","volume":"167","author":"Hoc","year":"2021","journal-title":"Eur. J. Pharm. Biopharm."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/j.supflu.2008.12.008","article-title":"Preparation of Starch-Based Scaffolds for Tissue Engineering by Supercritical Immersion Precipitation","volume":"49","author":"Duarte","year":"2009","journal-title":"J. Supercrit. Fluids"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1016\/j.ijpharm.2010.09.040","article-title":"Development of Biodegradable Porous Starch Foam for Improving Oral Delivery of Poorly Water Soluble Drugs","volume":"403","author":"Wu","year":"2011","journal-title":"Int. J. Pharm."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.jconrel.2016.11.009","article-title":"Solid Cellulose Nanofiber Based Foams\u2013Towards Facile Design of Sustained Drug Delivery Systems","volume":"244","author":"Svagan","year":"2016","journal-title":"J. Control. Release"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"20","DOI":"10.2174\/1875181401002020020","article-title":"Shape Memory Polymer Foams for Biomedical Devices","volume":"2","author":"Sokolowski","year":"2010","journal-title":"Open Med. Devices J."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1021\/ma901530r","article-title":"Polymer Scaffolds for Biomaterials Applications","volume":"43","author":"Shoichet","year":"2010","journal-title":"Macromolecules"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Lowinger, M.B., Barrett, S.E., Zhang, F., and Williams, R.O. (2018). Sustained Release Drug Delivery Applications of Polyurethanes. Pharmaceutics, 10.","DOI":"10.3390\/pharmaceutics10020055"},{"key":"ref_33","first-page":"S1063","article-title":"Efficiency of Castor Oil\u2013Based Polyurethane Foams for Oil Sorption S10 and S500: Influence of Porous Size and Statistical Analysis","volume":"29","author":"Alves","year":"2021","journal-title":"Polym. Polym. Compos."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"2239","DOI":"10.1007\/s00289-022-04155-9","article-title":"Polyurethane Foams from Vegetable Oil-Based Polyols: A Review","volume":"80","author":"Kaikade","year":"2023","journal-title":"Polym. Bull."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"2467","DOI":"10.1007\/s10924-017-1138-7","article-title":"Polyurethane Foams Based on Biopolyols from Castor Oil and Glycerol","volume":"26","author":"Bresolin","year":"2018","journal-title":"J. Polym. Environ."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.polymdegradstab.2018.03.022","article-title":"Rheological Properties, Oxidative and Thermal Stability, and Potential Application of Biopolyols Prepared via Two-Step Process from Crude Glycerol","volume":"152","author":"Hejna","year":"2018","journal-title":"Polym. Degrad. Stab."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Salcedo, M.L.D., Omisol, C.J.M., Maputi, A.O., Estrada, D.J.E., Aguinid, B.J.M., Asequia, D.M.A., Erjeno, D.J.D., Apostol, G., Siy, H., and Malaluan, R.M. (2023). Production of Bio-Based Polyol from Coconut Fatty Acid Distillate (CFAD) and Crude Glycerol for Rigid Polyurethane Foam Applications. Materials, 16.","DOI":"10.20944\/preprints202306.1719.v1"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.porgcoat.2014.11.017","article-title":"Isocyanate Terminated Castor Oil-Based Polyurethane Prepolymer: Synthesis and Characterization","volume":"80","author":"Gurunathan","year":"2015","journal-title":"Prog. Org. Coat."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1080\/14658011.2015.1112538","article-title":"Preparation and Properties of Rigid Polyurethane Foam Based on Modified Castor Oil","volume":"45","author":"Li","year":"2016","journal-title":"Plast. Rubber Compos."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1177\/0021955X12445178","article-title":"Structure and Properties of the Polyurethane and Polyurethane Foam Synthesized from Castor Oil Polyols","volume":"48","author":"Stirna","year":"2012","journal-title":"J. Cell. Plast."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Lee, J.H., Kim, S.H., and Oh, K.W. (2021). Bio-Based Polyurethane Foams with Castor Oil Based Multifunctional Polyols for Improved Compressive Properties. Polymers, 13.","DOI":"10.3390\/polym13040576"},{"key":"ref_42","first-page":"21","article-title":"Evaluation of the Antimicrobial and Phytochemical Properties of Oil from Castor Seeds (Ricinus Communis Linn)","volume":"1","author":"Momoh","year":"2012","journal-title":"Bull. Environ. Pharmacol. Life Sci."},{"key":"ref_43","first-page":"p2057","article-title":"Antifungal Activities of Black Seed, Castor and Lemon Oils against Pathogenic Plant Fungi","volume":"6","author":"Obisesan","year":"2022","journal-title":"Trop. J. Nat. Prod. Res."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"121","DOI":"10.4103\/aam.aam_56_18","article-title":"Inflammation and Cancer","volume":"18","author":"Singh","year":"2019","journal-title":"Ann. Afr. Med."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Sung, Y.K., and Kim, S.W. (2020). Recent Advances in Polymeric Drug Delivery Systems. Biomater. Res., 24.","DOI":"10.1186\/s40824-020-00190-7"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1023\/A:1016500429225","article-title":"Hyaluronic Acid and Silver Sulfadiazine-Impregnated Polyurethane Foams for Wound Dressing Application","volume":"13","author":"Cho","year":"2002","journal-title":"J. Mater. Sci. Mater. Med."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.ajps.2018.09.001","article-title":"Development of Polyurethane Foam Dressing Containing Silver and Asiaticoside for Healing of Dermal Wound","volume":"14","author":"Namviriyachote","year":"2018","journal-title":"Asian J. Pharm. Sci."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"2100114","DOI":"10.1002\/mame.202100114","article-title":"Fabrication of Biodegradable Polyurethane Foam Scaffolds with Customized Shapes and Controlled Mechanical Properties by Gas Foaming Technique","volume":"306","author":"Han","year":"2021","journal-title":"Macromol. Mater. Eng."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"853","DOI":"10.4103\/0973-1482.139267","article-title":"An Overview of Doxorubicin Formulations in Cancer Therapy","volume":"10","author":"Rivankar","year":"2014","journal-title":"J. Cancer Res. Ther."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"2398","DOI":"10.1016\/j.actbio.2009.03.036","article-title":"Simultaneous Drug Release at Different Rates from Biodegradable Polyurethane Foams","volume":"5","author":"Sivak","year":"2009","journal-title":"Acta Biomater."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Zhang, M., Zhu, J., Zheng, Y., Guo, R., Wang, S., Mignani, S., Caminade, A.-M., Majoral, J.-P., and Shi, X. (2018). Doxorubicin-Conjugated PAMAM Dendrimers for pH-Responsive Drug Release and Folic Acid-Targeted Cancer Therapy. Pharmaceutics, 10.","DOI":"10.3390\/pharmaceutics10030162"},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Szota, M., and Jachimska, B. (2023). Effect of Alkaline Conditions on Forming an Effective G4.0 PAMAM Complex with Doxorubicin. Pharmaceutics, 15.","DOI":"10.3390\/pharmaceutics15030875"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Marcinkowska, M., Sobierajska, E., Stanczyk, M., Janaszewska, A., Chworos, A., and Klajnert-Maculewicz, B. (2018). Conjugate of PAMAM Dendrimer, Doxorubicin and Monoclonal Antibody\u2014Trastuzumab: The New Approach of a Well-Known Strategy. Polymers, 10.","DOI":"10.3390\/polym10020187"},{"key":"ref_54","unstructured":"Fei, Z., Hongbin, Z., and Zinan, Z. (2024, May 22). Cross-Linking Agent, Rigid Polyurethane Foam Material and Preparation Method Thereof 2010. Available online: https:\/\/patents.justia.com\/patent\/10604615."},{"key":"ref_55","first-page":"4707","article-title":"In Vitro Cytotoxicity of the Ternary PAMAM G3-Pyridoxal-Biotin Bioconjugate","volume":"8","author":"Uram","year":"2013","journal-title":"Int. J. Nanomed."},{"key":"ref_56","unstructured":"(2010). Cellular Plastics and Rubbers-Determination of Apparent Density (ISO 845:2006) (Standard No. UNE EN ISO 845:2010). Available online: https:\/\/www.en-standard.eu\/une-en-iso-845-2010-cellular-plastics-and-rubbers-determination-of-apparent-density-iso-845-2006\/."},{"key":"ref_57","unstructured":"(2024, May 22). Standard Test Method for Water Absorption of Rigid Cellular Plastics. Available online: https:\/\/www.astm.org\/d2842-19.html."},{"key":"ref_58","unstructured":"(2020). Practice for Use of Calorimetric Dosimetry Systems for Dose Measurements and Dosimetry System Calibration in Electron Beams (Standard No. ISO\/ASTM 51631:2020)."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"518","DOI":"10.3349\/ymj.2002.43.4.518","article-title":"Evaluation of the Cytotoxicity of Polyetherurethane (PU) Film Containing Zinc Diethyldithiocarbamate (ZDEC) on Various Cell Lines","volume":"43","author":"Park","year":"2002","journal-title":"Yonsei Med. J."},{"key":"ref_60","unstructured":"US Pharmacopeia (2024, June 05). Chapter 87: Biological Reactivity Test, In Vitro. Available online: https:\/\/www.drugfuture.com\/pharmacopoeia\/usp32\/pub\/data\/v32270\/usp32nf27s0_c87.html."},{"key":"ref_61","unstructured":"(2021). Biological Evaluation of Medical devices-Part 12: Sample Preparation and Reference Materials (Standard No. ISO 10993-12)."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"706","DOI":"10.1016\/j.bmc.2016.11.047","article-title":"Cellular Uptake of Glucoheptoamidated Poly(Amidoamine) PAMAM G3 Dendrimer with Amide-Conjugated Biotin, a Potential Carrier of Anticancer Drugs","volume":"25","author":"Uram","year":"2017","journal-title":"Bioorg Med. Chem."},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Ar\u00e9valo-Alquichire, S., Valero, M., Ar\u00e9valo-Alquichire, S., and Valero, M. (2017). Castor Oil Polyurethanes as Biomaterials. Elastomers, IntechOpen.","DOI":"10.5772\/intechopen.68597"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"2068","DOI":"10.1016\/j.carbpol.2011.10.027","article-title":"Biocompatible Elastomer of Waterborne Polyurethane Based on Castor Oil and Polyethylene Glycol with Cellulose Nanocrystals","volume":"87","author":"Gao","year":"2012","journal-title":"Carbohydr. Polym."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"3959","DOI":"10.1002\/slct.202000784","article-title":"Reinstating Structural Stability of Castor Oil Based Flexible Polyurethane Foam Using Glycerol","volume":"5","author":"Jain","year":"2020","journal-title":"ChemistrySelect"},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Janaszewska, A., Lazniewska, J., Trzepi\u0144ski, P., Marcinkowska, M., and Klajnert-Maculewicz, B. (2019). Cytotoxicity of Dendrimers. Biomolecules, 9.","DOI":"10.3390\/biom9080330"},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Li, X., Naeem, A., Xiao, S., Hu, L., Zhang, J., and Zheng, Q. (2022). Safety Challenges and Application Strategies for the Use of Dendrimers in Medicine. Pharmaceutics, 14.","DOI":"10.3390\/pharmaceutics14061292"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/S1387-2656(05)11004-7","article-title":"Tetrazolium Dyes as Tools in Cell Biology: New Insights into Their Cellular Reduction","volume":"Volume 11","author":"Berridge","year":"2005","journal-title":"Biotechnology Annual Review"},{"key":"ref_69","doi-asserted-by":"crossref","unstructured":"Grz\u0119da, D., W\u0119grzyk, G., Nowak, A., Idaszek, J., Szczepkowski, L., and Ryszkowska, J. (2023). Cytotoxic Properties of Polyurethane Foams for Biomedical Applications as a Function of Isocyanate Index. Polymers, 15.","DOI":"10.3390\/polym15122754"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1177\/088532829100500404","article-title":"An Assessment of 2,4 TDA Formation from Surgitek Polyurethane Foam under Simulated Physiological Conditions","volume":"5","author":"Szycher","year":"1991","journal-title":"J. Biomater. Appl."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"7457","DOI":"10.1016\/j.biomaterials.2005.05.079","article-title":"Understanding the Biodegradation of Polyurethanes: From Classical Implants to Tissue Engineering Materials","volume":"35","author":"Santerre","year":"2005","journal-title":"Biomaterials"},{"key":"ref_72","first-page":"1083","article-title":"Biobased Polyurethanes for Biomedical Applications","volume":"6","author":"Wendels","year":"2021","journal-title":"Bioact. Mater."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"18","DOI":"10.12970\/2311-1755.2019.07.03","article-title":"Polymers and Scaffolds with Improved Blood Compatibility and Enhanced Cellular Response with Focus on Polyurethane Foams Functionalized with Amino-Amide Groups","volume":"7","author":"Tanzi","year":"2019","journal-title":"J. Adv. Biotechnol. Bioeng."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1002\/pst.426","article-title":"Guidelines for Accurate EC50\/IC50 Estimation","volume":"10","author":"Sebaugh","year":"2011","journal-title":"Pharmaceut. Statist."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"110356","DOI":"10.1016\/j.eurpolymj.2021.110356","article-title":"Advances in Development of the Dendrimers Having Natural Saccharides in Their Structure for Efficient and Controlled Drug Delivery Applications","volume":"148","author":"Pooresmaeil","year":"2021","journal-title":"Eur. Polym. J."},{"key":"ref_76","first-page":"191","article-title":"Evaluation of the Localization and Biological Effects of PAMAM G3 Dendrimer-Biotin\/Pyridoxal Conjugate as HaCaT Keratinocyte Targeted Nanocarrier","volume":"66","author":"Szuster","year":"2019","journal-title":"Acta Biochim. Pol."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"3305","DOI":"10.1166\/jnn.2014.7995","article-title":"Synthesis, Biodistribution, and Imaging of PEGylated-Acetylated Polyamidoamine Dendrimers","volume":"14","author":"Liu","year":"2014","journal-title":"J. Nanosci. Nanotechnol."},{"key":"ref_78","doi-asserted-by":"crossref","unstructured":"Tsujimoto, A., Uehara, H., Yoshida, H., Nishio, M., Furuta, K., Inui, T., Matsumoto, A., Morita, S., Tanaka, M., and Kojima, C. (2021). Different Hydration States and Passive Tumor Targeting Ability of Polyethylene Glycol-Modified Dendrimers with High and Low PEG Density. Mater. Sci. Eng. C Mater. Biol. Appl., 126.","DOI":"10.1016\/j.msec.2021.112159"},{"key":"ref_79","doi-asserted-by":"crossref","unstructured":"Peter, S., Alven, S., Maseko, R.B., and Aderibigbe, B.A. (2022). Doxorubicin-Based Hybrid Compounds as Potential Anticancer Agents: A Review. Molecules, 27.","DOI":"10.3390\/molecules27144478"},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"15475","DOI":"10.1039\/C7RA00713B","article-title":"A Poly(Amidoamine) Dendrimer-Based Drug Carrier for Delivering DOX to Gliomas Cells","volume":"7","author":"Li","year":"2017","journal-title":"RSC Adv."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"1882","DOI":"10.1080\/09205063.2021.1938859","article-title":"Dendrimer as a Promising Nanocarrier for the Delivery of Doxorubicin as an Anticancer Therapeutics","volume":"32","author":"Singh","year":"2021","journal-title":"J. Biomater. Sci. Polym. Ed."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"101424","DOI":"10.1016\/j.jddst.2019.101424","article-title":"Glucoheptoamidated Polyamidoamine PAMAM G3 Dendrimer as a Vehicle for Succinate Linked Doxorubicin; Enhanced Toxicity of DOX against Grade IV Glioblastoma U-118 MG Cells","volume":"55","author":"Tutaj","year":"2020","journal-title":"J. Drug Deliv. Sci. Technol."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1134\/S1607672910010072","article-title":"Expression Profiling and Putative Mechanisms of Resistance to Doxorubicin of Human Lung Cancer Cells","volume":"430","author":"Kashkin","year":"2010","journal-title":"Dokl. Biochem. Biophys."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1046\/j.0960-7722.2001.00214.x","article-title":"High Sensitivity of Human Epidermal Keratinocytes (HaCaT) to Topoisomerase Inhibitors","volume":"34","author":"Pessina","year":"2001","journal-title":"Cell Prolif."},{"key":"ref_85","unstructured":"(2024, June 05). Drug: Doxorubicin-Cancerrxgene-Genomics of Drug Sensitivity in Cancer. Available online: https:\/\/www.cancerrxgene.org\/compound\/Doxorubicin\/133\/by-tissue?tissue=CESC."},{"key":"ref_86","doi-asserted-by":"crossref","unstructured":"Szota, M., Szwedowicz, U., Rembialkowska, N., Janicka-Klos, A., Doveiko, D., Chen, Y., Kulbacka, J., and Jachimska, B. (2024). Dendrimer Platforms for Targeted Doxorubicin Delivery\u2014Physicochemical Properties in Context of Biological Responses. Int. J. Mol. Sci., 25.","DOI":"10.20944\/preprints202406.1710.v1"},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"1295","DOI":"10.1039\/D3ME00060E","article-title":"Describing the Adsorption of Doxorubicin on a PAMAM Dendrimer by Ab Initio Calculations","volume":"8","author":"Sobrinho","year":"2023","journal-title":"Mol. Syst. Des. Eng."},{"key":"ref_88","doi-asserted-by":"crossref","unstructured":"Wasiak, I., Kulikowska, A., Janczewska, M., Michalak, M., Cymerman, I.A., Nagalski, A., Kallinger, P., Szymanski, W.W., and Ciach, T. (2016). Dextran Nanoparticle Synthesis and Properties. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0146237"},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1016\/j.tranon.2014.07.003","article-title":"Co-Encapsulation of Doxorubicin with Galactoxyloglucan Nanoparticles for Intracellular Tumor-Targeted Delivery in Murine Ascites and Solid Tumors","volume":"7","author":"Joseph","year":"2014","journal-title":"Transl. Oncol."},{"key":"ref_90","first-page":"1","article-title":"Self-Assembly of a Disulfide-Containing Core\/Shell Nanocomplex with Intracellular Environment-Sensitive Facilitated Endo-Lysosomal Escape for Enhanced Antitumor Efficacy","volume":"56","author":"Li","year":"2021","journal-title":"J. Mater. Sci."},{"key":"ref_91","doi-asserted-by":"crossref","unstructured":"Ibrahim, M., Abuwatfa, W.H., Awad, N.S., Sabouni, R., and Husseini, G.A. (2022). Encapsulation, Release, and Cytotoxicity of Doxorubicin Loaded in Liposomes, Micelles, and Metal-Organic Frameworks: A Review. Pharmaceutics, 14.","DOI":"10.3390\/pharmaceutics14020254"},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.jconrel.2013.02.026","article-title":"A Simple Way to Enhance Doxil\u00ae Therapy: Drug Release from Liposomes at the Tumor Site by Amphiphilic Block Copolymer","volume":"168","author":"Zhao","year":"2013","journal-title":"J. Control. Release"},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1016\/j.jconrel.2013.08.034","article-title":"Intact Doxil Is Taken up Intracellularly and Released Doxorubicin Sequesters in the Lysosome: Evaluated by in Vitro\/in Vivo Live Cell Imaging","volume":"172","author":"Seynhaeve","year":"2013","journal-title":"J. Control. Release"},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"115722","DOI":"10.1016\/j.envres.2023.115722","article-title":"Doxorubicin-Loaded Micelles in Tumor Cell-Specific Chemotherapy","volume":"227","author":"Almajidi","year":"2023","journal-title":"Environ. Res."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.colsurfb.2015.04.003","article-title":"Construction and Evaluation of PAMAM-DOX Conjugates with Superior Tumor Recognition and Intracellular Acid-Triggered Drug Release Properties","volume":"136","author":"Cheng","year":"2015","journal-title":"Colloids Surf. 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