{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T10:49:13Z","timestamp":1787914153218,"version":"build-2784847793"},"reference-count":89,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2025,5,21]],"date-time":"2025-05-21T00:00:00Z","timestamp":1747785600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006595","name":"Ministry of Education and Research, CCCDI\u2014UEFISCDI","doi-asserted-by":"publisher","award":["PN-IV-P2-2.1-TE-2023-0993"],"award-info":[{"award-number":["PN-IV-P2-2.1-TE-2023-0993"]}],"id":[{"id":"10.13039\/501100006595","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006595","name":"Ministry of Education and Research, CCCDI\u2014UEFISCDI","doi-asserted-by":"publisher","award":["30N\/2023"],"award-info":[{"award-number":["30N\/2023"]}],"id":[{"id":"10.13039\/501100006595","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006595","name":"Ministry of Education and Research, CCCDI\u2014UEFISCDI","doi-asserted-by":"publisher","award":["PN 23 05 (PN11N-03-01-2023)"],"award-info":[{"award-number":["PN 23 05 (PN11N-03-01-2023)"]}],"id":[{"id":"10.13039\/501100006595","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Ministry of Education and Research under the Romanian National Nucleu Program LAPLAS VII","award":["PN-IV-P2-2.1-TE-2023-0993"],"award-info":[{"award-number":["PN-IV-P2-2.1-TE-2023-0993"]}]},{"name":"Ministry of Education and Research under the Romanian National Nucleu Program LAPLAS VII","award":["30N\/2023"],"award-info":[{"award-number":["30N\/2023"]}]},{"name":"Ministry of Education and Research under the Romanian National Nucleu Program LAPLAS VII","award":["PN 23 05 (PN11N-03-01-2023)"],"award-info":[{"award-number":["PN 23 05 (PN11N-03-01-2023)"]}]},{"name":"Core Program within the National Research Development and Innovation Plan 2022\u20132027","award":["PN-IV-P2-2.1-TE-2023-0993"],"award-info":[{"award-number":["PN-IV-P2-2.1-TE-2023-0993"]}]},{"name":"Core Program within the National Research Development and Innovation Plan 2022\u20132027","award":["30N\/2023"],"award-info":[{"award-number":["30N\/2023"]}]},{"name":"Core Program within the National Research Development and Innovation Plan 2022\u20132027","award":["PN 23 05 (PN11N-03-01-2023)"],"award-info":[{"award-number":["PN 23 05 (PN11N-03-01-2023)"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Materials"],"abstract":"<jats:p>The development of bioactive coatings for metallic implants is essential to enhance osseointegration and improve implant longevity. In this study, composite thin films based on bioactive glass and melittin were synthesized using the matrix-assisted pulsed laser evaporation technique and deposited onto titanium substrates. The coatings were characterized using physicochemical analysis methods, including scanning electron microscopy, atomic force microscopy, contact angle measurements, Fourier transform infrared spectroscopy, energy-dispersive X-ray spectroscopy, and electrochemical impedance spectroscopy. Simulated body fluid immersion tests were also conducted to assess bioactivity over time. Scanning electron microscopy and atomic force microscopy revealed dense, irregular surface textures with nanoscale features and an average roughness of ~120 nm, favorable for cell adhesion. Contact angle measurements showed a significant shift from hydrophobic (~95\u00b0 for bare titanium) to moderately hydrophilic (~62\u00b0 for the bioglass and melittin coating) surfaces, indicating improved biocompatibility. Electrochemical impedance spectroscopy demonstrated enhanced corrosion resistance in simulated body fluid, with the coating exhibiting a ~45% decrease in impedance magnitude after 12 h of immersion, compared to only 4% for bare titanium. Fourier transform infrared spectroscopy and energy-dispersive X-ray spectroscopy analyses confirmed the progressive formation of a carbonated apatite layer after 7 days of simulated body fluid exposure, suggesting high bioactivity and osteoconductive potential. The combined effects of bioactive glass and melittin in the thin film structure offer promising applications in orthopedic and dental implants, enhancing both biological performance and structural integrity.<\/jats:p>","DOI":"10.3390\/ma18102410","type":"journal-article","created":{"date-parts":[[2025,5,21]],"date-time":"2025-05-21T09:58:22Z","timestamp":1747821502000},"page":"2410","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Bioactive Glass and Melittin Thin Films Deposited by MAPLE for Titanium Implant Functionalization"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3149-6972","authenticated-orcid":false,"given":"Mihaela","family":"Dinu","sequence":"first","affiliation":[{"name":"National Institute of Research and Development for Optoelectronics\u2014INOE2000, 409 Atomistilor St., 077125 Magurele, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4102-635X","authenticated-orcid":false,"given":"Bogdan","family":"Bita","sequence":"additional","affiliation":[{"name":"National Institute of Research and Development for Optoelectronics\u2014INOE2000, 409 Atomistilor St., 077125 Magurele, Romania"},{"name":"Faculty of Physics, University of Bucharest, 405 Atomistilor, 077125 Magurele, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4661-8362","authenticated-orcid":false,"given":"Anca Constantina","family":"Parau","sequence":"additional","affiliation":[{"name":"National Institute of Research and Development for Optoelectronics\u2014INOE2000, 409 Atomistilor St., 077125 Magurele, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8433-9217","authenticated-orcid":false,"given":"Carmen","family":"Ristoscu","sequence":"additional","affiliation":[{"name":"National Institute for Laser, Plasma and Radiation Physics, 409 Atomistilor Street, P.O. Box MG 36, 077125 Magurele, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4038-7548","authenticated-orcid":false,"given":"Irina","family":"Negut","sequence":"additional","affiliation":[{"name":"National Institute for Laser, Plasma and Radiation Physics, 409 Atomistilor Street, P.O. Box MG 36, 077125 Magurele, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,5,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"5300","DOI":"10.1021\/acsbiomaterials.4c01032","article-title":"Tailored Bioactive Glass Coating: Navigating Devitrification Toward a Superior Implant Performance","volume":"10","author":"Chakraborty","year":"2024","journal-title":"ACS Biomater. Sci. Eng."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Xu, Z., Miao, L., Meng, X., Sui, J., Chen, M., Zheng, Z., Huo, S., Liu, S., and Zhang, H. (2024). Strontium-Doped Bioactive Glass-Functionalized Polyetheretherketone Enhances Osseointegration by Facilitating Cell Adhesion. Colloids Surf. B Biointerfaces, 241.","DOI":"10.1016\/j.colsurfb.2024.114042"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Avelino, S.d.O.M., Alvares Sobral-Silva, L., Thim, G.P., de Almeida-Silva, L.A., dos Santos Lupp, J., Campos, T.M.B., and de Vasconcellos, L.M.R. (2024). Development, Characterization, and Biological Study of Bioglass Coatings 45S5 and BioK on Zirconia Implant Surfaces. J. Biomed. Mater. Res. B Appl. Biomater., 112.","DOI":"10.1002\/jbm.b.35380"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"134970","DOI":"10.1016\/j.cej.2022.134970","article-title":"Synthesis, Bioactive Properties, and Biomedical Applications of Intrinsically Therapeutic Nanoparticles for Disease Treatment","volume":"435","author":"Nguyen","year":"2022","journal-title":"Chem. Eng. J."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"112315","DOI":"10.1016\/j.msec.2021.112315","article-title":"Hydrothermally Treated Titanium Surfaces for Enhanced Osteogenic Differentiation of Adipose Derived Stem Cells","volume":"128","author":"Manivasagam","year":"2021","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.actbio.2023.07.048","article-title":"Surface Modification of Titanium Implants with Mg-Containing Coatings to Promote Osseointegration","volume":"169","author":"Wang","year":"2023","journal-title":"Acta Biomater."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Homa, K., Zakrzewski, W., Dobrzy\u0144ski, W., Piszko, P.J., Piszko, A., Matys, J., Wiglusz, R.J., and Dobrzy\u0144ski, M. (2024). Surface Functionalization of Titanium-Based Implants with a Nanohydroxyapatite Layer and Its Impact on Osteoblasts: A Systematic Review. J. Funct. Biomater., 15.","DOI":"10.3390\/jfb15020045"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Tuikampee, S., Chaijareenont, P., Rungsiyakull, P., and Yavirach, A. (2024). Titanium Surface Modification Techniques to Enhance Osteoblasts and Bone Formation for Dental Implants: A Narrative Review on Current Advances. Metals, 14.","DOI":"10.3390\/met14050515"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1177\/00220345241231777","article-title":"Strategies for Improving Impaired Osseointegration in Compromised Animal Models","volume":"103","author":"Deng","year":"2024","journal-title":"J. Dent. Res."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Liang, J., Lu, X., Zheng, X., Li, Y.R., Geng, X., Sun, K., Cai, H., Jia, Q., Jiang, H.B., and Liu, K. (2023). Modification of Titanium Orthopedic Implants with Bioactive Glass: A Systematic Review of in Vivo and in Vitro Studies. Front. Bioeng. Biotechnol., 11.","DOI":"10.3389\/fbioe.2023.1269223"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1016\/j.jcis.2020.06.102","article-title":"Synthesis of Bioactive Glass-Based Coating by Plasma Electrolytic Oxidation: Untangling a New Deposition Pathway toward Titanium Implant Surfaces","volume":"579","author":"Costa","year":"2020","journal-title":"J. Colloid Interface Sci."},{"key":"ref_12","unstructured":"Zafar, M.S., Khurshid, Z., Khan, A.S., Najeeb, S., and Sefat, F. (2020). 6\u2014Bioactive Glass Coated Dental Implants. Dental Implants, Woodhead Publishing."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"30170","DOI":"10.1021\/acsami.0c07318","article-title":"Biofunctional and Tribomechanical Behavior of Porous Titanium Substrates Coated with a Bioactive Glass Bilayer (45S5\u20131393)","volume":"12","author":"Begines","year":"2020","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1203","DOI":"10.1039\/D2TB02129C","article-title":"A pH-Neutral Bioactive Glass Coated 3D-Printed Porous Ti6Al4V Scaffold with Enhanced Osseointegration","volume":"11","author":"Wang","year":"2023","journal-title":"J. Mater. Chem. B"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"124432","DOI":"10.1016\/j.matchemphys.2021.124432","article-title":"Melittin Antimicrobial Peptide Thin Layer on Bone Implant Chitosan-Antibiotic Coatings and Their Bactericidal Properties","volume":"263","author":"Zarghami","year":"2021","journal-title":"Mater. Chem. Phys."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1007\/s10856-022-06666-3","article-title":"Improving Bactericidal Performance of Implant Composite Coatings by Synergism between Melittin and Tetracycline","volume":"33","author":"Zarghami","year":"2022","journal-title":"J. Mater. Sci. Mater. Med."},{"key":"ref_17","first-page":"29","article-title":"Enhance the Biological Properties of Commercial Pure Titanium with Bioactive Glass Coating by Pulsed Laser Deposition","volume":"51","author":"Mohammed","year":"2021","journal-title":"J. Biomim. Biomater. Biomed. Eng."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Negut, I., Gradisteanu-Pircalabioru, G., Dinu, M., Bita, B., Parau, A.C., Grumezescu, V., Ristoscu, C., and Chifiriuc, M.C. (2023). Bioglass and Vitamin D3 Coatings for Titanium Implants: Osseointegration and Corrosion Protection. Biomedicines, 11.","DOI":"10.3390\/biomedicines11102772"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Askari, P., Namaei, M.H., Ghazvini, K., and Hosseini, M. (2021). In Vitro and in Vivo Toxicity and Antibacterial Efficacy of Melittin against Clinical Extensively Drug-Resistant Bacteria. BMC Pharmacol. Toxicol., 22.","DOI":"10.1186\/s40360-021-00503-z"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1802","DOI":"10.1021\/acs.jpcb.6b11207","article-title":"Dynamic Structure and Orientation of Melittin Bound to Acidic Lipid Bilayers, As Revealed by Solid-State NMR and Molecular Dynamics Simulation","volume":"121","author":"Norisada","year":"2017","journal-title":"J. Phys. Chem. B"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1016\/j.tibtech.2017.12.003","article-title":"Bioactive Glasses: Sprouting Angiogenesis in Tissue Engineering","volume":"36","author":"Kargozar","year":"2018","journal-title":"Trends Biotechnol."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Kowalski, J., Rylska, D., Januszewicz, B., Konieczny, B., Cichomski, M., Matinlinna, J.P., Radwanski, M., Sokolowski, J., and Lukomska-Szymanska, M. (2023). Corrosion Resistance of Titanium Dental Implant Abutments: Comparative Analysis and Surface Characterization. Materials, 16.","DOI":"10.3390\/ma16206624"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Robles, D., Brizuela, A., Fern\u00e1ndez-Dom\u00ednguez, M., and Gil, J. (2023). Corrosion Resistance and Titanium Ion Release of Hybrid Dental Implants. Materials, 16.","DOI":"10.3390\/ma16103650"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1002\/jbm.820240607","article-title":"Solutions Able to Reproduce in Vivo Surface-Structure Changes in Bioactive Glass-Ceramic A-W3","volume":"24","author":"Kokubo","year":"1990","journal-title":"J. Biomed. Mater. Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"7981","DOI":"10.1016\/j.apsusc.2007.02.146","article-title":"Bioactive Glass and Hydroxyapatite Thin Films Obtained by Pulsed Laser Deposition","volume":"253","author":"Gyorgy","year":"2007","journal-title":"Appl. Surf. Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1279","DOI":"10.1016\/j.apsusc.2007.08.009","article-title":"Synthesis of Functionally Graded Bioactive Glass-Apatite Multistructures on Ti Substrates by Pulsed Laser Deposition","volume":"254","author":"Tanaskovic","year":"2007","journal-title":"Appl. Surf. Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1002\/jor.25974","article-title":"Evaluation of Biocompatibility and Osseointegration of Multi-Component TiAl6V4 Titanium Alloy Implants","volume":"43","author":"Jacobs","year":"2025","journal-title":"J. Orthop. Res."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Wu, H., Chen, X., Kong, L., and Liu, P. (2023). Mechanical and Biological Properties of Titanium and Its Alloys for Oral Implant with Preparation Techniques: A Review. Materials, 16.","DOI":"10.3390\/ma16216860"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"124","DOI":"10.18231\/j.aprd.2024.024","article-title":"The Titanium Triumph: Exploring the Transformative World of Dental Implants","volume":"10","author":"Rohith","year":"2024","journal-title":"IP Ann. Prosthodont. Restor. Dent."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.actbio.2016.01.035","article-title":"High-Density Antimicrobial Peptide Coating with Broad Activity and Low Cytotoxicity against Human Cells","volume":"33","author":"Rai","year":"2016","journal-title":"Acta Biomater."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1104","DOI":"10.1016\/j.bcp.2007.10.029","article-title":"Melittin Exhibits Necrotic Cytotoxicity in Gastrointestinal Cells Which Is Attenuated by Cholesterol","volume":"75","author":"Maher","year":"2008","journal-title":"Biochem. Pharmacol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1016\/j.colsurfb.2016.03.037","article-title":"Characterization of Antimicrobial Activity against Listeria and Cytotoxicity of Native Melittin and Its Mutant Variants","volume":"143","author":"Wu","year":"2016","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1016\/j.lfs.2006.09.024","article-title":"Phospholipase A2-Independent Ca2+ Entry and Subsequent Apoptosis Induced by Melittin in Human MG63 Osteosarcoma Cells","volume":"80","author":"Chu","year":"2007","journal-title":"Life Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"55902","DOI":"10.1021\/acsami.1c17618","article-title":"Stable Loading and Delivery of Melittin with Lipid-Coated Polymeric Nanoparticles for Effective Tumor Therapy with Negligible Systemic Toxicity","volume":"13","author":"Ye","year":"2021","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_35","first-page":"16","article-title":"Imprinting Bone Surface Topography on Cast Metal","volume":"1","author":"Demir","year":"2024","journal-title":"Bull. Mater. Sci. Metall."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1007\/s10856-023-06733-3","article-title":"Beta-TCP Scaffolds with Rationally Designed Macro-Micro Hierarchical Structure Improved Angio\/Osteo-Genesis Capability for Bone Regeneration","volume":"34","author":"Feng","year":"2023","journal-title":"J. Mater. Sci. Mater. Med."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"\u00d6zcolak, B., Erenay, B., Odaba\u015f, S., Jandt, K.D., and Garipcan, B. (2024). Effects of Bone Surface Topography and Chemistry on Macrophage Polarization. Sci. Rep., 14.","DOI":"10.1038\/s41598-024-62484-3"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"103","DOI":"10.2147\/MDER.S360297","article-title":"Effects of Titanium Implant Surface Topology on Bone Cell Attachment and Proliferation in Vitro","volume":"15","author":"Levin","year":"2022","journal-title":"Med. Devices Evid. Res."},{"key":"ref_39","first-page":"1235","article-title":"Glass Bone Implants: The Effect of Surface Topology on Attachment and Proliferation of Osteoblast Cells on 45S Bioactive Glass","volume":"1235","author":"Jain","year":"2009","journal-title":"MRS Online Proc. Libr. OPL"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/j.jmst.2024.04.032","article-title":"Indirect 3D Printing CDHA Scaffolds with Hierarchical Porous Structure to Promote Osteoinductivity and Bone Regeneration","volume":"207","author":"Dai","year":"2025","journal-title":"J. Mater. Sci. Technol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"115215","DOI":"10.1063\/5.0223608","article-title":"Cell Migration Behavior Regulation by Nanopillars and Oxide Coating","volume":"14","author":"Liu","year":"2024","journal-title":"AIP Adv."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"110673","DOI":"10.1016\/j.compositesb.2023.110673","article-title":"Surface-Based Modified 3D-Printed BG\/GO Scaffolds Promote Bone Defect Repair through Bone Immunomodulation","volume":"257","author":"Qi","year":"2023","journal-title":"Compos. Part B Eng."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"2310607","DOI":"10.1002\/adfm.202310607","article-title":"Single- and Multiscale Laser Patterning of 3D Printed Biomedical Titanium Alloy: Toward an Enhanced Adhesion and Early Differentiation of Human Bone Marrow Stromal Cells","volume":"34","author":"Hariharan","year":"2024","journal-title":"Adv. Funct. Mater."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1007\/s10856-024-06794-y","article-title":"Optimizing Alkaline Hydrothermal Treatment for Biomimetic Smart Metallic Orthopedic and Dental Implants","volume":"35","author":"Hadady","year":"2024","journal-title":"J. Mater. Sci. Mater. Med."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"10639","DOI":"10.2147\/IJN.S488154","article-title":"Elucidating the Mechanism of Large-Diameter Titanium Dioxide Nanotubes in Protecting Osteoblasts Under Oxidative Stress Environment: The Role of Fibronectin and Albumin Adsorption","volume":"19","author":"Xiang","year":"2024","journal-title":"Int. J. Nanomed."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1102","DOI":"10.1177\/09544119231188297","article-title":"Theoretical Modeling Approach for Adsorption of Fibronectin on the Nanotopographical Implants","volume":"237","author":"Gao","year":"2023","journal-title":"Proc. Inst. Mech. Eng. Part H"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"55232","DOI":"10.1021\/acsami.3c06749","article-title":"Lithium-Modified TiO2 Surface by Anodization for Enhanced Protein Adsorption and Cell Adhesion","volume":"15","author":"Wu","year":"2023","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"3893","DOI":"10.1039\/D2BM02165J","article-title":"Manganese Supplementation of Orthopedic Implants: A New Strategy for Enhancing Integrin-Mediated Cellular Responses","volume":"11","author":"Li","year":"2023","journal-title":"Biomater. Sci."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"640","DOI":"10.1002\/jbm.a.20075","article-title":"Osteoblast Attachment and Mineralized Nodule Formation on Rough and Smooth 45S5 Bioactive Glass Monoliths","volume":"68A","author":"Gough","year":"2004","journal-title":"J. Biomed. Mater. Res. A"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1007\/s10989-024-10600-2","article-title":"In Situ Assembly of Melittin-PHA Microspheres for Enhancing Therapeutic Efficacy in Cancer Treatment","volume":"30","author":"Fan","year":"2024","journal-title":"Int. J. Pept. Res. Ther."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"2300569","DOI":"10.1002\/ardp.202300569","article-title":"Immunomodulatory Activities and Biomedical Applications of Melittin and Its Recent Advances","volume":"357","author":"Jafari","year":"2024","journal-title":"Arch. Pharm."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"124129","DOI":"10.1016\/j.molliq.2024.124129","article-title":"Structural and Conductivity Properties of Lipid-Coated Melittin Peptide Nanowires Molded at Air-Water Interface","volume":"397","author":"Pino","year":"2024","journal-title":"J. Mol. Liq."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"339","DOI":"10.4236\/ampc.2020.1012027","article-title":"Polydopamine Coating on Titanium Affects Osteoblastic Differentiation to a Greater Degree than Does Surface Roughness","volume":"10","author":"Latifi","year":"2020","journal-title":"Adv. Mater. Phys. Chem."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"4779","DOI":"10.1021\/acs.langmuir.3c03639","article-title":"Characterization of Surface Modifications in Oxygen Plasma-Treated Teflon AF1600","volume":"40","author":"Xiang","year":"2024","journal-title":"Langmuir"},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Dwivedi, S., Dixit, A.R., Das, A.K., and Srivastava, A.K. (2023). Time-Dependent Wetting Behavior of the Micro-Textured Stainless Steel 316L Using the Mechanical Indentation Method. Proc. Inst. Mech. Eng. Part E.","DOI":"10.1177\/09544089231205782"},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Gao, P., MacKay, I., Gruber, A., Krantz, J., Piccolo, L., Lucchetta, G., Pelaccia, R., Orazi, L., and Masato, D. (2023). Wetting Characteristics of Laser-Ablated Hierarchical Textures Replicated by Micro Injection Molding. Micromachines, 14.","DOI":"10.3390\/mi14040863"},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Lazauskas, A., Andrulevi\u010dius, M., Abakevi\u010dien\u0117, B., Jucius, D., Grigali\u016bnas, V., Guobien\u0117, A., and Me\u0161kinis, \u0160. (2023). Hydrophilic Surface Modification of Amorphous Hydrogenated Carbon Nanocomposite Films via Atmospheric Oxygen Plasma Treatment. Nanomaterials, 13.","DOI":"10.3390\/nano13061108"},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Surmeneva, M., Nikityuk, P., Hans, M., and Surmenev, R. (2016). Deposition of Ultrathin Nano-Hydroxyapatite Films on Laser Micro-Textured Titanium Surfaces to Prepare a Multiscale Surface Topography for Improved Surface Wettability\/Energy. Materials, 9.","DOI":"10.3390\/ma9110862"},{"key":"ref_59","unstructured":"Allain, J.P., Echeverry-Rend\u00f3n, M., Pav\u00f3n, J.J., and Arias, S.L. (2015). Nanostructured Biointerfaces. Nanopatterning and Nanoscale Devices for Biological Applications, CRC Press."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1007\/s10971-024-06355-3","article-title":"A Hydrophilic Nanocomposite Coating with Enhanced Durability and Antifouling Properties","volume":"110","author":"Lu","year":"2024","journal-title":"J. Sol-Gel Sci. Technol."},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"de Oliveira, D.P., Micocci, K.C., de Almeida, G.F.B., Otuka, A.J.G., Mendon\u00e7a, C.R., Selistre-de-Araujo, H.S., and Bolfarini, C. (2023). Comparison of Hydrophilic and Hydrophobic Nano Topographic Surfaces of Titanium Alloys on Pre-Osteoblastic Cell Interaction. Biomed. Phys. Eng. Express, 9.","DOI":"10.1088\/2057-1976\/acd786"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"e12915","DOI":"10.1111\/eos.12915","article-title":"A Novel Bioactive Glass\/Graphene Oxide Composite Coating for a Polyether Ether Ketone-Based Dental Implant","volume":"131","author":"Rawlinson","year":"2023","journal-title":"Eur. J. Oral Sci."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"082501","DOI":"10.1149\/1945-7111\/ace9ff","article-title":"Influence of Mesoporous Bioactive Glass Particles Doped with Cu and Mg on the Microstructure and Properties of Zein-Based Coatings Obtained by Electrophoretic Deposition","volume":"170","author":"Moskalewicz","year":"2023","journal-title":"J. Electrochem. Soc."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"129824","DOI":"10.1016\/j.surfcoat.2023.129824","article-title":"Coating of Bioactive Glasses with Chitosan: The Effects of the Glass Composition and Coating Method on the Surface Properties, Including Preliminary in Vitro Results","volume":"470","author":"Spriano","year":"2023","journal-title":"Surf. Coat. Technol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"2103","DOI":"10.1016\/S0142-9612(00)00145-9","article-title":"Passive Film on Orthopaedic TiAlV Alloy Formed in Physiological Solution Investigated by X-Ray Photoelectron Spectroscopy","volume":"21","author":"Strehblow","year":"2000","journal-title":"Biomaterials"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"4961","DOI":"10.1021\/acs.jpcc.5b12490","article-title":"Surface Reactions of Mesoporous Bioactive Glasses Monitored by Solid-State NMR: Concentration Effects in Simulated Body Fluid","volume":"120","author":"Stevensson","year":"2016","journal-title":"J. Phys. Chem. C"},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Zurita-M\u00e9ndez, N.N., Carbajal-De la Torre, G., Flores-Merino, M.V., and Espinosa-Medina, M.A. (2022). Development of Bioactive Glass-Collagen-Hyaluronic Acid-Polycaprolactone Scaffolds for Tissue Engineering Applications. Front. Bioeng. Biotechnol., 10.","DOI":"10.3389\/fbioe.2022.825903"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"035404","DOI":"10.1088\/2053-1591\/aab2f5","article-title":"Corrosion Resistance and In-Vitro Bioactivity of BaO Containing Na2O-CaO-P2O5 Phosphate Glass-Ceramic Coating Prepared on 316 L, Duplex Stainless Steel 2205 and Ti6Al4V","volume":"5","author":"Edathazhe","year":"2018","journal-title":"Mater. Res. Express"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"123265","DOI":"10.1016\/j.jnoncrysol.2024.123265","article-title":"Mathematical Modeling of Bioactive Glass Degradation","volume":"646","author":"Chen","year":"2024","journal-title":"J. Non-Cryst. Solids"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1016\/j.electacta.2016.12.087","article-title":"Relationship between Constant-Phase Element (CPE) Parameters and Physical Properties of Films with a Distributed Resistivity","volume":"225","year":"2017","journal-title":"Electrochim. Acta"},{"key":"ref_71","doi-asserted-by":"crossref","unstructured":"Meng, Y., Yang, Y., Zhang, C., Cui, X., Liu, E., Jin, G., Kang, J., and She, P. (2023). Influence of Bioactive Glass Addition on TC4 Laser Cladding Coatings: Microstructure and Electrochemical Properties. Coatings, 13.","DOI":"10.3390\/coatings13091621"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"1098","DOI":"10.1039\/c3ay26476a","article-title":"Electrochemical Impedance Spectroscopy: An Overview of Bioanalytical Applications","volume":"5","author":"Randviir","year":"2013","journal-title":"Anal. Methods"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"1487","DOI":"10.1111\/j.1151-2916.1991.tb07132.x","article-title":"Bioceramics: From Concept to Clinic","volume":"74","author":"Hench","year":"1991","journal-title":"J. Am. Ceram. Soc."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"6473","DOI":"10.1007\/s00289-020-03425-8","article-title":"Characterization of Sodium Alginate Containing Bioactive Glass Coatings Prepared by Sol\u2013Gel Processing","volume":"78","author":"Civan","year":"2021","journal-title":"Polym. Bull."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"19084","DOI":"10.1016\/j.ceramint.2024.03.006","article-title":"Mesoporous Bioactive Scaffolds Based on the 14.6Li2O\u22c58.6ZrO2\u22c567.3SiO2\u22c59.5Al2O3 Glass-Ceramic as Drug Delivery for Bone Regeneration","volume":"50","author":"Mattos","year":"2024","journal-title":"Ceram. Int."},{"key":"ref_76","doi-asserted-by":"crossref","unstructured":"Andersson, \u00d6.H., Happonen, R.-P., and Yli-Urpo, A. (1994). Short-Term Reaction Kinetics of Bioactive Glass in Simulated Body Fluid and in Subcutaneous Tissue. Bioceramics, Pergamon.","DOI":"10.1016\/B978-0-08-042144-5.50014-5"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"43","DOI":"10.2978\/jsas.18.43","article-title":"The Bioactivity Evaluation of Bioacitive Glass Coated Titanium Surface with Simulated Body Fluid","volume":"18","author":"Kawai","year":"2006","journal-title":"J. Adv. Sci."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"1921","DOI":"10.1080\/10426500213440","article-title":"Direct Observation of Apatite Formation on Bioglass in Simulated Body Fluid by Atomic Force Microscopy","volume":"177","author":"Hamagami","year":"2002","journal-title":"Phosphorus Sulfur Silicon Relat. Elem."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1002\/(SICI)1097-4636(19990315)44:4<416::AID-JBM7>3.0.CO;2-S","article-title":"XRD, SEM-EDS, and FTIR Studies of in Vitro Growth of an Apatite-like Layer on Sol-Gel Glasses","volume":"44","author":"Romero","year":"1999","journal-title":"J. Biomed. Mater. Res."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"2919","DOI":"10.1007\/s10856-007-3308-y","article-title":"Bioactive Glass Induced in Vitro Apatite Formation on Composite GBR Membranes","volume":"19","author":"Tirri","year":"2008","journal-title":"J. Mater. Sci. Mater. Med."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"519","DOI":"10.4028\/www.scientific.net\/KEM.309-311.519","article-title":"Nanostructured Apatite Coatings for Rapid Bone Repair","volume":"309\u2013311","author":"Thian","year":"2006","journal-title":"Key Eng. Mater."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"4635","DOI":"10.1016\/j.biomaterials.2006.04.026","article-title":"Nanoparticulate Drug Carriers Based on Hybrid Poly(d,l-Lactide-Co-Glycolide)-Dendron Structures","volume":"27","author":"Costantino","year":"2006","journal-title":"Biomaterials"},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1002\/jbm.b.31831","article-title":"Direct-Write Bioprinting Three-Dimensional Biohybrid Systems for Future Regenerative Therapies","volume":"98B","author":"Chang","year":"2011","journal-title":"J. Biomed. Mater. Res. B Appl. Biomater."},{"key":"ref_84","first-page":"1555","article-title":"A New Concept of Stainless Steel Medical Implant Based upon Composite Nanostructures Coating","volume":"9","author":"Floroian","year":"2014","journal-title":"Dig. J. Nanomater. Biostruct"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.jelechem.2010.08.005","article-title":"Double Layered Nanostructured Composite Coatings with Bioactive Silicate Glass and Polymethylmetacrylate for Biomimetic Implant Applications","volume":"648","author":"Floroian","year":"2010","journal-title":"J. Electroanal. Chem."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1016\/j.bbrc.2010.02.138","article-title":"Melittin Induces Apoptotic Features in Candida albicans","volume":"394","author":"Park","year":"2010","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1016\/j.colsurfb.2014.09.037","article-title":"Pore Formation in 1,2-Dimyristoyl-Sn-Glycero-3-Phosphocholine\/Cholesterol Mixed Bilayers by Low Concentrations of Antimicrobial Peptide Melittin","volume":"123","author":"Zhou","year":"2014","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"6755","DOI":"10.1039\/C5SM01562F","article-title":"Effect of Antimicrobial Peptide on the Dynamics of Phosphocholine Membrane: Role of Cholesterol and Physical State of Bilayer","volume":"11","author":"Sharma","year":"2015","journal-title":"Soft Matter"},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"106267","DOI":"10.1016\/j.phrs.2022.106267","article-title":"Biological Effects of Cannabidiol on Human Cancer Cells: Systematic Review of the Literature","volume":"181","author":"Valenti","year":"2022","journal-title":"Pharmacol. Res."}],"container-title":["Materials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1996-1944\/18\/10\/2410\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:36:37Z","timestamp":1760031397000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1996-1944\/18\/10\/2410"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5,21]]},"references-count":89,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2025,5]]}},"alternative-id":["ma18102410"],"URL":"https:\/\/doi.org\/10.3390\/ma18102410","relation":{},"ISSN":["1996-1944"],"issn-type":[{"value":"1996-1944","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,5,21]]}}}