{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T07:02:56Z","timestamp":1792479776608,"version":"4.5.0"},"reference-count":66,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2025,6,21]],"date-time":"2025-06-21T00:00:00Z","timestamp":1750464000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003196","name":"Italian Ministry of Health","doi-asserted-by":"publisher","award":["RF-2018-12368274"],"award-info":[{"award-number":["RF-2018-12368274"]}],"id":[{"id":"10.13039\/501100003196","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Materials"],"abstract":"<jats:p>X-ray imaging of articular cartilage could be a breakthrough for the early diagnosis of tissue degeneration. This approach relies on radiopaque contrast agents to enhance the visualization of soft tissues. The potential impact of contrast agents on the mechanical response of articular cartilage should be considered in the frame of both clinical and research applications. Attention has been drawn to a solution containing molecules with six iodine atoms and four positive charges (CA4+), which has been shown to improve the X-ray visibility of articular cartilage. This study aimed to determine the effects of a CA4+ solution on tissues\u2019 mechanical properties. An experimental pipeline based on indentation tests was applied to paired samples of articular cartilage before and after the immersion in either CA4+ or phosphate-buffered saline solution, maintained at a temperature of 22 \u00b1 2 \u00b0C, for 22 h to determine the differences in instantaneous, viscous, and equilibrium responses between the articular cartilage of the two groups. The 22 h immersion of articular cartilage in either CA4+ or phosphate-buffered saline solution had a significant detrimental effect on the overall response, including the instantaneous, viscous, and equilibrium responses, of the articular cartilage. However, this detrimental effect was greater with exposure to the CA4+ solution. Specifically, the articular cartilage was found to be less stiff in both the instantaneous response (approximately \u221225%) and the equilibrium response (approximately \u221238%). The softening effect could be attributable to an alteration of the interaction between the proteoglycans of articular cartilage, induced by the positive charges within the CA4+ contrast agent. Further investigations are needed to elucidate whether this hypothesized mechanism is reversible.<\/jats:p>","DOI":"10.3390\/ma18132943","type":"journal-article","created":{"date-parts":[[2025,6,23]],"date-time":"2025-06-23T04:50:05Z","timestamp":1750654205000},"page":"2943","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Assessment of CA4+ Impact on Mechanical Properties of Articular Cartilage"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9113-9451","authenticated-orcid":false,"given":"Matteo","family":"Berni","sequence":"first","affiliation":[{"name":"Laboratorio di Tecnologia Medica, IRCCS Istituto Ortopedico Rizzoli, 40136 Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1459-3356","authenticated-orcid":false,"given":"Simone","family":"Fantoni","sequence":"additional","affiliation":[{"name":"Laboratorio di Tecnologia Medica, IRCCS Istituto Ortopedico Rizzoli, 40136 Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberta","family":"Fognani","sequence":"additional","affiliation":[{"name":"Laboratorio di Tecnologia Medica, IRCCS Istituto Ortopedico Rizzoli, 40136 Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4177-6756","authenticated-orcid":false,"given":"Paolo","family":"Cardarelli","sequence":"additional","affiliation":[{"name":"Division of Ferrara, Istituto Nazionale di Fisica Nucleare (INFN), 44122 Ferrara, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8784-5652","authenticated-orcid":false,"given":"Fabio","family":"Baruffaldi","sequence":"additional","affiliation":[{"name":"Laboratorio di Tecnologia Medica, IRCCS Istituto Ortopedico Rizzoli, 40136 Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9759-6474","authenticated-orcid":false,"given":"Massimiliano","family":"Baleani","sequence":"additional","affiliation":[{"name":"Laboratorio di Tecnologia Medica, IRCCS Istituto Ortopedico Rizzoli, 40136 Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Eschweiler, J., Horn, N., Rath, B., Betsch, M., Baroncini, A., Tingart, M., and Migliorini, F. 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