{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T07:36:21Z","timestamp":1769758581965,"version":"3.49.0"},"reference-count":85,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2025,6,22]],"date-time":"2025-06-22T00:00:00Z","timestamp":1750550400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004442","name":"National Science Centre, Poland","doi-asserted-by":"publisher","award":["2021\/43\/B\/ST8\/01854"],"award-info":[{"award-number":["2021\/43\/B\/ST8\/01854"]}],"id":[{"id":"10.13039\/501100004442","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Materials"],"abstract":"<jats:p>This research investigates the biochemical and microbiological characteristics of a composite comprising poly(lactide) (PLA) combined with polyethersulfone (PESf) and copper-complexed cellulose phosphate (CelP-Cu). The material was produced using the pneumothermic melt-blown method and then modified with polyethersulfone and cellulose phosphate, followed by complexation with copper ions using the dip-coating technique. Comprehensive physicochemical and biological evaluations were conducted to characterize the composite. The physicochemical assessments involved elemental analysis (C, O, Cu) and morphology examination. The biological evaluations encompassed microbiological testing and biochemical\u2013hematological analysis, including activated partial thromboplastin time (aPTT) and prothrombin time (PT). Antimicrobial activity was assessed according to the EN ISO 20645:2006 and EN 14119:2005 standards, by placing material specimens on agar plates inoculated with representative microorganisms. The results revealed that the composites exhibited significant antimicrobial effects against model microorganisms: Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Bacillus atrophaeus, Candida albicans, Saccharomyces cerevisiae, Aspergillus niger, Chaetomium globosum. This study highlights the potential of PLA\/PESf\/CelP-Cu composites for novel biomedical applications, demonstrating their biocompatibility and their influence on hemostatic processes and antimicrobial properties.<\/jats:p>","DOI":"10.3390\/ma18132954","type":"journal-article","created":{"date-parts":[[2025,6,23]],"date-time":"2025-06-23T04:50:05Z","timestamp":1750654205000},"page":"2954","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Preparation and Biochemical and Microbial Behavior of Poly(Lactide) Composites with Polyethersulfone and Copper-Complexed Cellulose Phosphate"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1161-8343","authenticated-orcid":false,"given":"Marcin H.","family":"Kudzin","sequence":"first","affiliation":[{"name":"\u0141ukasiewicz Research Network\u2014Lodz Institute of Technology, 19\/27 Marii Sklodowskiej-Curie Street, 90-570 Lodz, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zdzis\u0142awa","family":"Mrozi\u0144ska","sequence":"additional","affiliation":[{"name":"\u0141ukasiewicz Research Network\u2014Lodz Institute of Technology, 19\/27 Marii Sklodowskiej-Curie Street, 90-570 Lodz, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2295-6460","authenticated-orcid":false,"given":"Anna","family":"Kaczmarek","sequence":"additional","affiliation":[{"name":"\u0141ukasiewicz Research Network\u2014Lodz Institute of Technology, 19\/27 Marii Sklodowskiej-Curie Street, 90-570 Lodz, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6227-9428","authenticated-orcid":false,"given":"Jerzy J.","family":"Chru\u015bciel","sequence":"additional","affiliation":[{"name":"\u0141ukasiewicz Research Network\u2014Lodz Institute of Technology, 19\/27 Marii Sklodowskiej-Curie Street, 90-570 Lodz, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martyna","family":"Gloc","sequence":"additional","affiliation":[{"name":"\u0141ukasiewicz Research Network\u2014Lodz Institute of Technology, 19\/27 Marii Sklodowskiej-Curie Street, 90-570 Lodz, Poland"},{"name":"Department of Bioprocess Engineering, Faculty of Process and Environmental Engineering, Lodz Univeristy of Technology, 213 Wolczanska Street, 90-924 Lodz, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0103-9428","authenticated-orcid":false,"given":"Renata","family":"\u017by\u0142\u0142a","sequence":"additional","affiliation":[{"name":"\u0141ukasiewicz Research Network\u2014Lodz Institute of Technology, 19\/27 Marii Sklodowskiej-Curie Street, 90-570 Lodz, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Wasy\u0142eczko, M., Wojciechowski, C., and Chwojnowski, A. (2024). Polyethersulfone Polymer for Biomedical Applications and Biotechnology. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25084233"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"3117","DOI":"10.2147\/DDDT.S165440","article-title":"Current Development of Biodegradable Polymeric Materials for Biomedical Applications","volume":"12","author":"Song","year":"2018","journal-title":"Drug Des. Dev. Ther."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"185","DOI":"10.3109\/21691401.2016.1146731","article-title":"Biodegradable and Biocompatible Polymers for Tissue Engineering Application: A Review","volume":"45","author":"Asghari","year":"2017","journal-title":"Artif. Cells Nanomed. Biotechnol."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Gobi, R., Ravichandiran, P., Babu, R.S., and Yoo, D.J. (2021). Biopolymer and Synthetic Polymer-Based Nanocomposites in Wound Dressing Applications: A Review. Polymers, 13.","DOI":"10.3390\/polym13121962"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"40593","DOI":"10.1002\/app.40593","article-title":"Physical and Thermal Characterization of Polylactic Acid Meltblown Nonwovens","volume":"131","author":"Hammonds","year":"2014","journal-title":"J. Appl. Polym. Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1002\/ppap.201000153","article-title":"Plasma Surface Modification of Biodegradable Polymers: A Review","volume":"8","author":"Morent","year":"2011","journal-title":"Plasma Process. Polym."},{"key":"ref_7","first-page":"204","article-title":"PLLA Scaffold Modification Using Magnetron Sputtering of the Copper Target to Provide Antibacterial Properties","volume":"3","author":"Badaraev","year":"2017","journal-title":"Resour.-Effic. Technol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1016\/j.addr.2016.06.012","article-title":"Physical and Mechanical Properties of PLA, and Their Functions in Widespread Applications\u2014A Comprehensive Review","volume":"107","author":"Farah","year":"2016","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_9","first-page":"76","article-title":"Applications of PLA in Modern Medicine","volume":"1","author":"DeStefano","year":"2020","journal-title":"Eng. Regen."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/j.ijpharm.2013.03.038","article-title":"Nanostructured Poly(l-Lactide) Matrix as Novel Platform for Drug Delivery","volume":"448","author":"Zhou","year":"2013","journal-title":"Int. J. Pharm."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2572","DOI":"10.26452\/ijrps.v10i3.1512","article-title":"Recent Advancement in Wound Healing Dressing Material","volume":"10","author":"Pandey","year":"2019","journal-title":"Int. J. Pharm. Sci. Res."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Sanyang, M.L., and Jawaid, M. (2019). Polylactic Acid (PLA)-Based Nanocomposites: Processing and Properties. Bio-Based Polymers and Nanocomposites: Preparation, Processing, Properties & Performance, Springer International Publishing.","DOI":"10.1007\/978-3-030-05825-8"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Alam, F., Shukla, V.R., Varadarajan, K.M., and Kumar, S. (2020). Microarchitected 3D Printed Polylactic Acid (PLA) Nanocomposite Scaffolds for Biomedical Applications. J. Mech. Behav. Biomed. Mater., 103.","DOI":"10.1016\/j.jmbbm.2019.103576"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Essa, D., Kondiah, P.P.D., Choonara, Y.E., and Pillay, V. (2020). The Design of Poly(Lactide-Co-Glycolide) Nanocarriers for Medical Applications. Front. Bioeng. Biotechnol., 8.","DOI":"10.3389\/fbioe.2020.00048"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Wu, D., Spanou, A., Diez-Escudero, A., and Persson, C. (2020). 3D-Printed PLA\/HA Composite Structures as Synthetic Trabecular Bone: A Feasibility Study Using Fused Deposition Modeling. J. Mech. Behav. Biomed. Mater., 103.","DOI":"10.1016\/j.jmbbm.2019.103608"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"944","DOI":"10.1080\/03602559.2014.979507","article-title":"Polylactic Acid in Medicine","volume":"54","author":"Davachi","year":"2015","journal-title":"Polym.-Plast. Technol. Eng."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"435","DOI":"10.3144\/expresspolymlett.2015.42","article-title":"Properties and Medical Applications of Polylactic Acid: A Review","volume":"9","author":"Hamad","year":"2015","journal-title":"Express Polym. Lett."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1016\/j.addr.2016.04.019","article-title":"Poly(Lactic Acid) Nanofibrous Scaffolds for Tissue Engineering","volume":"107","author":"Santoro","year":"2016","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1007\/s10439-015-1455-8","article-title":"An Overview of Mechanical Properties and Material Modeling of Polylactide (PLA) for Medical Applications","volume":"44","author":"Hayman","year":"2016","journal-title":"Ann. Biomed. Eng."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/j.addr.2016.03.012","article-title":"Biocompatibility and Safety of PLA and Its Copolymers","volume":"107","author":"Ramot","year":"2016","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1065","DOI":"10.1002\/ceat.201300477","article-title":"Polyethersulfone\/Poly(D,L-Lactide) Blend Membranes: Preparation, Characterization, and Performance","volume":"37","author":"Hassani","year":"2014","journal-title":"Chem. Eng. Technol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1016\/j.progpolymsci.2009.12.003","article-title":"Poly(Lactic Acid) Modifications","volume":"35","author":"Rasal","year":"2010","journal-title":"Prog. Polym. Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"574","DOI":"10.1016\/j.msec.2015.06.035","article-title":"Overview of PES Biocompatible\/Hemodialysis Membranes: PES\u2013Blood Interactions and Modification Techniques","volume":"56","author":"Irfan","year":"2015","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.colsurfb.2012.05.004","article-title":"Improved Blood Compatibility of Polyethersulfone Membrane with a Hydrophilic and Anionic Surface","volume":"100","author":"Nie","year":"2012","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"100013","DOI":"10.1016\/j.advmem.2021.100013","article-title":"Hemocompatibility Enhancement of Polyethersulfone Membranes: Strategies and Challenges","volume":"1","author":"Song","year":"2021","journal-title":"Adv. Membr."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1016\/j.jcis.2012.07.076","article-title":"Heparin-like Surface Modification of Polyethersulfone Membrane and Its Biocompatibility","volume":"386","author":"Tang","year":"2012","journal-title":"J. Colloid Interface Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2000717","DOI":"10.1002\/adma.202000717","article-title":"Recent Progress on Cellulose-Based Ionic Compounds for Biomaterials","volume":"33","author":"Yang","year":"2021","journal-title":"Adv. Mater."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"728","DOI":"10.1016\/j.reactfunctpolym.2005.10.027","article-title":"Cellulose Phosphates as Biomaterials. In Vitro Biocompatibility Studies","volume":"66","author":"Granja","year":"2006","journal-title":"React. Funct. Polym."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"3341","DOI":"10.1002\/app.2193","article-title":"Cellulose Phosphates as Biomaterials. I. Synthesis and Characterization of Highly Phosphorylated Cellulose Gels","volume":"82","author":"Granja","year":"2001","journal-title":"J. Appl. Polym. Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"3354","DOI":"10.1002\/app.2194","article-title":"Cellulose Phosphates as Biomaterials. II. Surface Chemical Modification of Regenerated Cellulose Hydrogels","volume":"82","author":"Granja","year":"2001","journal-title":"J. Appl. Polym. Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"273","DOI":"10.3109\/10731198409118836","article-title":"Blood-Cellulosics Interactions","volume":"12","author":"Sinha","year":"1984","journal-title":"Biomater. Med. Devices Artif. Organs"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2719","DOI":"10.1016\/S0142-9612(01)00013-8","article-title":"Anticoagulant Potential of Regioselective Derivatized Cellulose","volume":"22","author":"Groth","year":"2001","journal-title":"Biomaterials"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2163","DOI":"10.1023\/A:1017587815583","article-title":"Cellulose Phosphates as Biomaterials. Mineralization of Chemically Modified Regenerated Cellulose Hydrogels","volume":"36","author":"Granja","year":"2001","journal-title":"J. Mater. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Schimper, C.B., Pachschw\u00f6ll, P., Maitz, M.F., Werner, C., Rosenau, T., and Liebner, F. (2023). Hemocompatibility of Cellulose Phosphate Aerogel Membranes with Potential Use in Bone Tissue Engineering. Front. Bioeng. Biotechnol., 11.","DOI":"10.3389\/fbioe.2023.1152577"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Kudzin, M.H., Mrozi\u0144ska, Z., and Urbaniak, P. (2021). Vapor Phosphorylation of Cellulose by Phosphorus Trichlo-Ride: Selective Phosphorylation of 6-Hydroxyl Function\u2014The Synthesis of New Antimicrobial Cellulose 6-Phosphate(III)-Copper Complexes. Antibiotics, 10.","DOI":"10.3390\/antibiotics10020203"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1016\/j.mam.2005.07.015","article-title":"Copper, Oxidative Stress, and Human Health","volume":"26","author":"Keen","year":"2005","journal-title":"Mol. Aspects Med."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/S0300-483X(03)00159-8","article-title":"Copper Toxicity, Oxidative Stress, and Antioxidant Nutrients","volume":"189","author":"Gaetke","year":"2003","journal-title":"Toxicology"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1016\/j.surfcoat.2014.06.036","article-title":"Copper(I)Oxide Surface Modified Cellulose Fibers\u2014Synthesis, Characterization and Antimicrobial Properties","volume":"254","author":"Emam","year":"2014","journal-title":"Surf. Coat. Technol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2506","DOI":"10.1002\/adfm.201100123","article-title":"Copper-Based Nanostructured Coatings on Natural Cellulose: Nanocomposites Exhibiting Rapid and Efficient Inhibition of a Multi-Drug Resistant Wound Pathogen, A. Baumannii, and Mammalian Cell Biocompatibility In Vitro","volume":"21","author":"Cady","year":"2011","journal-title":"Adv. Funct. Mater."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1016\/j.ijheh.2016.06.003","article-title":"Antimicrobial Applications of Copper","volume":"219","author":"Vincent","year":"2016","journal-title":"Int. J. Hyg. Environ. Health"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1001","DOI":"10.1007\/s00253-013-5422-8","article-title":"Bioactivity, Mechanism of Action, and Cytotoxicity of Copper-Based Nanoparticles: A Review","volume":"98","author":"Ingle","year":"2014","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1728","DOI":"10.1096\/fj.04-2029fje","article-title":"Putting Copper into Action: Copper-Impregnated Products with Potent Biocidal Activities","volume":"18","author":"Borkow","year":"2004","journal-title":"FASEB J."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.surfcoat.2013.07.009","article-title":"Effect of Copper and Copper Alginate Treatment on Wool Fabric. Study of Textile and Antibacterial Properties","volume":"235","author":"Heliopoulos","year":"2013","journal-title":"Surf. Coat. Technol."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Kudzin, M.H., Kaczmarek, A., Mrozi\u0144ska, Z., and Olczyk, J. (2020). Deposition of Copper on Polyester Knitwear Fibers by a Magnetron Sputtering System. Physical Properties and Evaluation of Antimicrobial Response of New Multi-Functional Composite Materials. Appl. Sci., 10.","DOI":"10.3390\/app10196990"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Kudzin, M.H., Bogu\u0144, M., Mrozi\u0144ska, Z., and Kaczmarek, A. (2020). Physical Properties, Chemical Analysis, and Evaluation of Antimicrobial Response of New Polylactide\/Alginate\/Copper Composite Materials. Mar. Drugs, 18.","DOI":"10.3390\/md18120660"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Kudzin, M.H., Mrozi\u0144ska, Z., Kaczmarek, A., and Lisiak-Kuci\u0144ska, A. (2020). Deposition of Copper on Poly(Lactide) Non-Woven Fabrics by Magnetron Sputtering\u2014Fabrication of New Multi-Functional, Antimicrobial Composite Materials. Materials, 13.","DOI":"10.3390\/ma13183971"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Slavin, Y.N., Asnis, J., H\u00e4feli, U.O., and Bach, H. (2017). Metal Nanoparticles: Understanding the Mechanisms behind Antibacterial Activity. J. Nanobiotechnol., 15.","DOI":"10.1186\/s12951-017-0308-z"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"S\u00e1nchez-L\u00f3pez, E., Gomes, D., Esteruelas, G., Bonilla, L., Lopez-Machado, A.L., Galindo, R., Cano, A., Espina, M., Ettcheto, M., and Camins, A. (2020). Metal-Based Nanoparticles as Antimicrobial Agents: An Overview. Nanomaterials, 10.","DOI":"10.3390\/nano10020292"},{"key":"ref_49","unstructured":"Ficai, A., and Grumezescu, A.M. (2017). Chapter 23\u2014Copper-Based Nanoparticles as Antimicrobials. Nanostructures for Antimicrobial Therapy, Elsevier. Micro and Nano Technologies."},{"key":"ref_50","first-page":"193","article-title":"Chapter Six\u2014Copper and Antibiotics: Discovery, Modes of Action, and Opportunities for Medicinal Applications","volume":"Volume 70","author":"Poole","year":"2017","journal-title":"Advances in Microbial Physiology"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"135101","DOI":"10.1088\/0957-4484\/25\/13\/135101","article-title":"Mechanism of Antibacterial Activity of Copper Nanoparticles","volume":"25","author":"Chatterjee","year":"2014","journal-title":"Nanotechnology"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1032","DOI":"10.1111\/jam.13681","article-title":"Contact Killing and Antimicrobial Properties of Copper","volume":"124","author":"Vincent","year":"2018","journal-title":"J. Appl. Microbiol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"100192","DOI":"10.1016\/j.impact.2019.100192","article-title":"Copper Nanoparticles Have Negligible Direct Antibacterial Impact","volume":"17","author":"Bastos","year":"2020","journal-title":"NanoImpact"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Ramos-Z\u00fa\u00f1iga, J., Bruna, N., and P\u00e9rez-Donoso, J.M. (2023). Toxicity Mechanisms of Copper Nanoparticles and Copper Surfaces on Bacterial Cells and Viruses. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms241310503"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"49402","DOI":"10.1021\/acsami.1c11236","article-title":"High-Resolution Microscopical Studies of Contact Killing Mechanisms on Copper-Based Surfaces","volume":"13","author":"Chang","year":"2021","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1541","DOI":"10.1128\/AEM.02766-10","article-title":"Metallic Copper as an Antimicrobial Surface","volume":"77","author":"Grass","year":"2011","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.surfcoat.2009.06.028","article-title":"CuO\u2013Cotton Nanocomposite: Formation, Morphology, and Antibacterial Activity","volume":"204","author":"Perelshtein","year":"2009","journal-title":"Surf. Coat. Technol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/j.carbpol.2017.02.044","article-title":"In-Situ Deposition of Cu 2 O Micro-Needles for Biologically Active Textiles and Their Release Properties","volume":"165","author":"Emam","year":"2017","journal-title":"Carbohydr. Polym."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"E319","DOI":"10.1002\/app.36981","article-title":"Copper Nanoparticles Loaded Alginate-Impregnated Cotton Fabric with Antibacterial Properties","volume":"126","author":"Bajpai","year":"2012","journal-title":"J. Appl. Polym. Sci."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"684","DOI":"10.1016\/j.bbrc.2019.07.110","article-title":"Copper Nanoparticles Promote Rapid Wound Healing in Acute Full Thickness Defect via Acceleration of Skin Cell Migration, Proliferation, and Neovascularization","volume":"517","author":"Alizadeh","year":"2019","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jinorgbio.2016.02.012","article-title":"The Neglected Role of Copper Ions in Wound Healing","volume":"161","author":"Kornblatt","year":"2016","journal-title":"J. Inorg. Biochem."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1111\/j.1524-475X.2010.00573.x","article-title":"Molecular Mechanisms of Enhanced Wound Healing by Copper Oxide-Impregnated Dressings","volume":"18","author":"Borkow","year":"2010","journal-title":"Wound Repair Regen."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"H1821","DOI":"10.1152\/ajpheart.01015.2001","article-title":"Copper-Induced Vascular Endothelial Growth Factor Expression and Wound Healing","volume":"282","author":"Sen","year":"2002","journal-title":"Am. J. Physiol.-Heart Circ. Physiol."},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Cucci, L.M., Satriano, C., Marzo, T., and La Mendola, D. (2021). Angiogenin and Copper Crossing in Wound Healing. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms221910704"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1049\/iet-nbt.2013.0052","article-title":"Biosynthesis and Wound Healing Activity of Copper Nanoparticles","volume":"8","author":"Tiwari","year":"2014","journal-title":"IET Nanobiotechnol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1111\/j.1365-3164.2006.00551.x","article-title":"Evaluation of the Effects of Topical Tripeptide-Copper Complex and Zinc Oxide on Open-Wound Healing in Rabbits","volume":"17","author":"Cangul","year":"2006","journal-title":"Vet. Dermatol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"2108","DOI":"10.1111\/j.1538-7836.2012.04890.x","article-title":"Immobilized Transition Metals Stimulate Contact Activation and Drive Factor XII-Mediated Coagulation","volume":"10","author":"Mutch","year":"2012","journal-title":"J. Thromb. Haemost."},{"key":"ref_68","unstructured":"(2025, March 10). Elmer: Analytical Methods for Atomic Absorption Spectroscopy\u2014Google Scholar. Available online: https:\/\/scholar.google.com\/scholar_lookup?title=Analytical+Methods+for+Atomic+Absorption+Spectroscopy&author=The+Perkin%E2%80%93Elmer+Corporation&publication_year=1996."},{"key":"ref_69","unstructured":"(2006). Textile Fabrics\u2014Determination of Antibacterial Activity\u2014Agar Diffusion Plate Test (Standard No. EN ISO 20645:2006)."},{"key":"ref_70","unstructured":"(2005). Testing of Textiles\u2014Evaluation of the Action of Microfungi\u2014Visual Method (Standard No. PN-EN 14119:2005 Point 10.5 (B2))."},{"key":"ref_71","doi-asserted-by":"crossref","unstructured":"Kiel, A., Kaltschmidt, B.P., Asghari, E., H\u00fctten, A., Kaltschmidt, B., and Kaltschmidt, C. (2022). Bacterial Biofilm Formation on Nano-Copper Added PLA Suited for 3D Printed Face Masks. Microorganisms, 10.","DOI":"10.3390\/microorganisms10020439"},{"key":"ref_72","doi-asserted-by":"crossref","unstructured":"Ahmed, W., Al-Marzouqi, A.H., Nazir, M.H., Rizvi, T.A., Zaneldin, E., Khan, M., and Aziz, M. (2023). Investigating the Properties and Characterization of a Hybrid 3D Printed Antimicrobial Composite Material Using FFF Process: Innovative and Swift. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24108895"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1021\/acsbiomaterials.6b00766","article-title":"Development and Antibacterial Performance of Novel Polylactic Acid-Graphene Oxide-Silver Nanoparticle Hybrid Nanocomposite Mats Prepared By Electrospinning","volume":"3","author":"Liu","year":"2017","journal-title":"ACS Biomater. Sci. Eng."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"282","DOI":"10.4103\/0975-7406.72127","article-title":"Introduction to Metallic Nanoparticles","volume":"2","author":"Mody","year":"2010","journal-title":"J. Pharm. Bioallied Sci."},{"key":"ref_75","doi-asserted-by":"crossref","unstructured":"Uzair, B., Liaqat, A., Iqbal, H., Menaa, B., Razzaq, A., Thiripuranathar, G., Fatima Rana, N., and Menaa, F. (2020). Green and Cost-Effective Synthesis of Metallic Nanoparticles by Algae: Safe Methods for Translational Medicine. Bioengineering, 7.","DOI":"10.3390\/bioengineering7040129"},{"key":"ref_76","doi-asserted-by":"crossref","unstructured":"Chu, Z., Zhao, T., Li, L., Fan, J., and Qin, Y. (2017). Characterization of Antimicrobial Poly (Lactic Acid)\/Nano-Composite Films with Silver and Zinc Oxide Nanoparticles. Materials, 10.","DOI":"10.3390\/ma10060659"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"025014","DOI":"10.1088\/2043-6254\/ab92f9","article-title":"Antimicrobial Ultrathin Film Based on Well-Defined Silver Nanoparticles and Polylactide","volume":"11","author":"Hoang","year":"2020","journal-title":"Adv. Nat. Sci. Nanosci. Nanotechnol."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1016\/j.jddst.2017.10.005","article-title":"In Vitro Study and Characterization of Cotton Fabric PLA Composite as a Slow Antibiotic Delivery Device for Biomedical Applications","volume":"43","author":"Macha","year":"2018","journal-title":"J. Drug Deliv. Sci. Technol."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1007\/s40138-013-0014-6","article-title":"Anticoagulants: A Review of the Pharmacology, Dosing, and Complications","volume":"1","author":"Alquwaizani","year":"2013","journal-title":"Curr. Emerg. Hosp. Med. Rep."},{"key":"ref_80","unstructured":"Rountree, K.M., Yaker, Z., and Lopez, P.P. (2025). Partial Thromboplastin Time. StatPearls, StatPearls Publishing."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"1531","DOI":"10.1515\/CCLM.2009.339","article-title":"Age-Associated Developmental Changes in the Activated Partial Thromboplastin Time (APTT) and Causes of Prolonged APTT Values in Healthy Chinese Children","volume":"47","author":"Li","year":"2009","journal-title":"Clin. Chem. Lab. Med."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1002\/app.24192","article-title":"In Vitro Comparative Hemostatic Studies of Chitin, Chitosan, and Their Derivatives","volume":"102","author":"Janvikul","year":"2006","journal-title":"J. Appl. Polym. Sci."},{"key":"ref_83","doi-asserted-by":"crossref","unstructured":"Jiao, S., Zhang, X., Cai, H., Wu, S., Ou, X., Han, G., Zhao, J., Li, Y., Guo, W., and Liu, T. (2023). Recent Advances in Biomimetic Hemostatic Materials. Mater. Today Bio, 19.","DOI":"10.1016\/j.mtbio.2023.100592"},{"key":"ref_84","doi-asserted-by":"crossref","unstructured":"Mrozi\u0144ska, Z., Kudzin, M.H., Ponczek, M.B., Kaczmarek, A., Kr\u00f3l, P., Lisiak-Kuci\u0144ska, A., \u017by\u0142\u0142a, R., and Walawska, A. (2024). Biochemical Approach to Poly(Lactide)\u2013Copper Composite\u2014Impact on Blood Coagulation Processes. Materials, 17.","DOI":"10.3390\/ma17030608"},{"key":"ref_85","doi-asserted-by":"crossref","unstructured":"Mrozi\u0144ska, Z., Ponczek, M., Kaczmarek, A., Bogu\u0144, M., Sulak, E., and Kudzin, M.H. (2023). Blood Coagulation Activities of Cotton\u2013Alginate\u2013Copper Composites. Mar. 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