{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T22:38:26Z","timestamp":1792103906253,"version":"4.4.0"},"reference-count":233,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T00:00:00Z","timestamp":1783382400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Materials"],"abstract":"<jats:p>Tea tree essential oil (TTO), extracted from Melaleuca alternifolia, is a terpene-rich botanical antimicrobial with demonstrated broad-spectrum activity against foodborne pathogens and spoilage microorganisms. Its bioactivity is principally attributed to oxygenated monoterpenes, most notably including terpinen-4-ol, \u03b3-terpinene, and \u03b1-terpinene, whose structure\u2013activity relationships govern interactions with microbial membranes and intracellular targets. This review provides a comprehensive, mechanistically grounded analysis of TTO as a sustainable antimicrobial platform for food preservation applications. The physicochemical determinants of TTO performance are critically assessed, encompassing chemotype-dependent compositional variability, hydrophobicity, limited aqueous solubility, and oxidative instability, with emphasis on how these properties constrain efficacy in complex food matrices. Antimicrobial mechanisms are systematically examined, including membrane permeabilization, disruption of cellular homeostasis, oxidative stress induction, and quorum-sensing interference. Focus is placed on nanostructured delivery systems, including nanoemulsions, biopolymer-based encapsulants, and hybrid nanocomposites, that improve physicochemical stability, modulate release kinetics, and potentiate antimicrobial activity. The integration of these engineered formulations into edible coatings, active packaging, and sanitation protocols across fresh produce, meat, and dairy systems is evaluated in the context of practical food safety applications. Translational limitations are addressed, including volatility, sensory incompatibility, regulatory constraints, and concentration-dependent cytotoxicity considerations. Collectively, this review positions TTO-based nanoformulations as a scientifically promising and technologically scalable approach to next-generation food preservation, while identifying critical gaps that must be resolved to support regulatory acceptance and commercial implementation.<\/jats:p>","DOI":"10.3390\/ma19132915","type":"journal-article","created":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T09:53:46Z","timestamp":1783418026000},"page":"2915","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Nano-Enabled Advances in Tea Tree Essential Oil (Melaleuca alternifolia): Composition, Bioactivity, and Emerging Roles in Food Protection"],"prefix":"10.3390","volume":"19","author":[{"given":"Huy Loc","family":"Nguyen","sequence":"first","affiliation":[{"name":"Department of Engineering and Technology, Van Hien University, Ho Chi Minh City 72508, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1118-4322","authenticated-orcid":false,"given":"Hong Minh Xuan","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering and Food Technology, Nong Lam University, Ho Chi Minh City 71308, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-7788-3733","authenticated-orcid":false,"given":"Thi Bich Ngoc","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Department of Water Management and Hydrological Sciences, Texas A&M University, College Station, TX 77843-2117, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Iacovelli, F., Romeo, A., Lattanzio, P., Ammendola, S., Battistoni, A., La Frazia, S., Vindigni, G., Unida, V., Biocca, S., and Gaziano, R. (2023). Deciphering the broad antimicrobial activity of Melaleuca alternifolia tea tree oil by combining experimental and computational investigations. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms241512432"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Visan, I.A., and Negut, I. (2024). Coatings based on essential oils for combating antibiotic resistance. Antibiotics, 13.","DOI":"10.3390\/antibiotics13070625"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"e35815","DOI":"10.1016\/j.heliyon.2024.e35815","article-title":"Clean-label alternatives for food preservation: An emerging trend","volume":"10","author":"Chauhan","year":"2024","journal-title":"Heliyon"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1080\/10412905.2016.1232665","article-title":"Tea tree oil: A promising essential oil","volume":"29","author":"Yadav","year":"2017","journal-title":"J. Essent. Oil Res."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"106010","DOI":"10.1016\/j.porgcoat.2020.106010","article-title":"Chitosan-tea tree oil nanoemulsion and calcium chloride tailored edible coating increase the shelf life of fresh cut red bell pepper","volume":"151","author":"Sathiyaseelan","year":"2020","journal-title":"Prog. Org. Coat."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"e70141","DOI":"10.1002\/nano.70141","article-title":"Organic nanoparticles in food systems as antimicrobial agents: Synthesis strategies, functional properties, and biofilm inhibition mechanisms","volume":"7","author":"Nguyen","year":"2026","journal-title":"Nano Sel."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1128\/CMR.19.1.50-62.2006","article-title":"Melaleuca alternifolia (Tea tree) oil: A Review of antimicrobial and other medicinal properties","volume":"19","author":"Carson","year":"2006","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Yu, J. (2025). Chemical composition of essential oils and their potential applications in postharvest storage of cereal grains. Molecules, 30.","DOI":"10.3390\/molecules30030683"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Cen, C., Wang, X., Li, H., Miao, S., Chen, J., and Wang, Y. (2025). Nano-emulsification potentiates tea tree oil bioactivity: High-stability formulation for dual antimicrobial and antioxidant food preservation. Foods, 14.","DOI":"10.3390\/foods14193405"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Puva\u010da, N., Milenkovic, J., Coghill, T.G., Bursic, V., Petrovic, A., Tanaskovic, S., Pelic, M., Pelic, D.L., and Miljkovic, T. (2021). Antimicrobial activity of selected essential oils against selected pathogenic bacteria: In vitro study. Antibiotics, 10.","DOI":"10.3390\/antibiotics10050546"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Nguyen, L., DeVico, B., Mannan, M., Chang, M., Santacruz, C.R., Siragusa, C., Everhart, S., and Fazen, C.H. (2023). Tea tree essential oil kills Escherichia coli and Staphylococcus epidermidis persisters. Biomolecules, 13.","DOI":"10.3390\/biom13091404"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1111\/jfs.70031","article-title":"Antibacterial effectiveness of zeolitic imidazolate framework-8 (ZIF-8) nanoparticle solutions and its derivatives against Salmonella Typhimurium ATCC13311 on loose-leaf lettuce","volume":"45","author":"Nguyen","year":"2025","journal-title":"J. Food Saf."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Karatas, H., Eker, F., Akda\u015f\u00e7i, E., Bechelany, M., and Karav, S. (2026). Silver nanoparticles in antibacterial research: Mechanisms, applications, and emerging perspectives. Int. J. Mol. Sci., 27.","DOI":"10.3390\/ijms27020927"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"6300","DOI":"10.1021\/jf070094x","article-title":"Interaction of four monoterpenes contained in essential oils with model membranes: Implications for their antibacterial activity","volume":"55","author":"Cristani","year":"2007","journal-title":"J. Agric. Food Chem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1038\/s44259-024-00046-3","article-title":"Mechanisms of antimicrobial resistance in biofilms","volume":"2","author":"Liu","year":"2024","journal-title":"npj Antimicrob. Resist."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Manzanelli, F.A., Ravetti, S., Brignone, S., Garro, A.G., Martinez, S., Vallejo, M.G., and Palma, S.D. (2023). Enhancing the functional properties of tea tree oil: In vitro antimicrobial activity and microencapsulation strategy. Pharmaceutics, 15.","DOI":"10.3390\/pharmaceutics15102489"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1080\/01140671.2016.1269813","article-title":"Bio-sanitation treatment using essential oils against E. coli O157:H7 on fresh lettuce","volume":"45","author":"Mouatcho","year":"2017","journal-title":"N. Z. J. Crop Hortic. Sci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"102171","DOI":"10.1016\/j.fochx.2025.102171","article-title":"Natural biopolymers in edible coatings: Applications in food preservation","volume":"25","author":"Karnwal","year":"2025","journal-title":"Food Chem. X"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1166","DOI":"10.1021\/acsfoodscitech.2c00070","article-title":"Biodegradable active packaging with controlled release: Principles, progress, and prospects","volume":"2","author":"Westlake","year":"2022","journal-title":"ACS Food Sci. Technol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"305","DOI":"10.48130\/fia-0024-0028","article-title":"Nanoencapsulation of volatile plant essential oils: A paradigm shift in food industry practices","volume":"3","author":"Sheikh","year":"2024","journal-title":"Food Innov. Adv."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/j.tiv.2017.09.007","article-title":"Chemical stability and in chemico reactivity of 24 fragrance ingredients of concern for skin sensitization risk assessment","volume":"46","author":"Avonto","year":"2018","journal-title":"Toxicol. In Vitro"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1302","DOI":"10.1016\/j.foodcont.2011.02.004","article-title":"Antimicrobial activity of polysaccharide films containing essential oils","volume":"22","author":"Chiralt","year":"2011","journal-title":"Food Control"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Latorre, R., Valerii, M.C., Benati, M., Lewis, R.E., Spigarelli, R., Bernacchi, A., Lippi, G., Spisni, E., and Gaibani, P. (2025). Lights and Shadows of Essential Oil-Derived Compounds: Antimicrobial and anti-inflammatory properties of eugenol, thymol, cinnamaldehyde, and carvacrol. Curr. Issues Mol. Biol., 47.","DOI":"10.3390\/cimb47110915"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"110784","DOI":"10.1016\/j.colsurfb.2020.110784","article-title":"Long-lasting anti-bacterial activity and bacteriostatic mechanism of tea tree oil adsorbed on the amino-functionalized mesoporous silica-coated by PAA","volume":"188","author":"Gao","year":"2020","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Yasin, M., Younis, A., Javed, T., Akram, A., Ahsan, M., Shabbir, R., Ali, M.M., Tahir, A., El-Ballat, E.M., and Sheteiwy, M.S. (2021). River tea tree oil: Composition, antimicrobial and antioxidant activities, and potential applications in agriculture. Plants, 10.","DOI":"10.3390\/plants10102105"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Mondello, F., Fontana, S., Scaturro, M., Girolamo, A., Colone, M., Stringaro, A., Di Vito, M., and Ricci, M.L. (2022). Terpinen-4-ol, the main bioactive component of tea tree oil, as an innovative antimicrobial agent against Legionella pneumophila. Pathogens, 11.","DOI":"10.3390\/pathogens11060682"},{"key":"ref_27","first-page":"224","article-title":"Preparation and characterization of tea tree oil-\u03b2-cyclodextrin microcapsules with super-high encapsulation efficiency","volume":"8","author":"Kong","year":"2023","journal-title":"J. Bioresour. Bioprod."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1111\/cod.12591","article-title":"Tea tree oil: Contact allergy and chemical composition","volume":"75","author":"Schmidt","year":"2016","journal-title":"Contact Dermat."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.foodcont.2018.11.013","article-title":"\u03b1-terpineol and terpene-4-ol, the critical components of tea tree oil, exert antifungal activities in vitro and in vivo against Aspergillus niger in grapes by inducing morphous damage and metabolic changes of fungus","volume":"98","author":"An","year":"2018","journal-title":"Food Control"},{"key":"ref_30","unstructured":"(2007). Oil of Melaleuca, Terpinen-4-ol Type (Tea Tree Oil) (Standard No. ISO 4730:2004)."},{"key":"ref_31","first-page":"336","article-title":"A promising bioactive component terpinene-4-ol: A review","volume":"1","author":"Yadav","year":"2016","journal-title":"Int. J. Pharmacogn."},{"key":"ref_32","unstructured":"Ul-Haq, I., Khan, S., Sohail, M., Iqbal, M.J., Awan, K.A., and Nayik, G.A. (2023). Chapter 20\u2014Tea tree essential oil. Essential Oils. Extraction, Characterization and Applications, Academic Press."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"100217","DOI":"10.1016\/j.fochx.2022.100217","article-title":"Terpenes and terpenoids as main bioactive compounds of essential oils, their roles in human health and potential application as natural food preservatives","volume":"13","author":"Masyita","year":"2022","journal-title":"Food Chem. X"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"3989","DOI":"10.3390\/molecules17043989","article-title":"Essential oils in combination and their antimicrobial properties","volume":"17","author":"Juliani","year":"2012","journal-title":"Molecules"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1016\/S0305-1978(99)00071-X","article-title":"Natural variation in the essential oil content of Melaleuca alternifolia Cheel (Myrtaceae)","volume":"28","author":"Homer","year":"2000","journal-title":"Biochem. Syst. Ecol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1016\/S0305-1978(01)00092-8","article-title":"Geographic variation in the essential oils and morphology of natural populations of Melaleuca alternifolia (Myrtaceae)","volume":"30","author":"Lee","year":"2002","journal-title":"Biochem. Syst. Ecol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1097\/DER.0b013e31823e202d","article-title":"Tea tree oil","volume":"23","author":"Larson","year":"2012","journal-title":"Dermatitis"},{"key":"ref_38","first-page":"727595","article-title":"The use of ATR-FTIR spectroscopy for quantification of adsorbed compounds","volume":"2015","author":"Rytwo","year":"2015","journal-title":"J. Spectrosc."},{"key":"ref_39","unstructured":"Ventos (2026, March 15). Tea Tree Oil. Available online: https:\/\/www.ventos.com\/index.php\/en\/producto\/1349\/TEA+TREE+OIL\/223."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"11955","DOI":"10.1039\/D3SC01630G","article-title":"Strategies for chiral separation: From racemate to enantiomer","volume":"14","author":"Sui","year":"2023","journal-title":"Chem. Sci."},{"key":"ref_41","unstructured":"Batt, C.A., and Tortorello, M.L. (2014). PRESERVATIVES|Traditional preservatives\u2013vegetable oils. Encyclopedia of Food Microbiology, Academic Press. [2nd ed.]."},{"key":"ref_42","unstructured":"European Chemicals Agency (2026, March 15). Melaleuca alternifolia, Ext. Available online: https:\/\/echa.europa.eu\/substance-information\/-\/substanceinfo\/100.077.588."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/j.ijpharm.2014.09.039","article-title":"Rhamnolipids as emulsifying agents for essential oil formulations: Antimicrobial effect against Candida albicans and methicillin-resistant Staphylococcus aureus","volume":"476","author":"Haba","year":"2014","journal-title":"Int. J. Pharm."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"4688","DOI":"10.1002\/fsn3.3431","article-title":"Impact of various extraction methods on fatty acid profile, physicochemical properties, and nutritional quality index of Pangus fish oil","volume":"11","author":"Rahman","year":"2023","journal-title":"Food Sci. Nutr."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"996","DOI":"10.1016\/j.molliq.2017.06.099","article-title":"Terpenes solubility in water and their environmental distribution","volume":"241","author":"Martins","year":"2017","journal-title":"J. Mol. Liq."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Matussek, F., Pavinatto, A., Knospe, P., Beuermann, S., and Sanfelice, R.C. (2022). Controlled release of tea tree oil from a chitosan matrix containing gold nanoparticles. Polymers, 14.","DOI":"10.3390\/polym14183808"},{"key":"ref_47","unstructured":"Scientific Committee on Consumer Safety (SCCS) (2025). Scientific Opinion on Tea Tree Oil (CAS\/EC No. 68647-73-4\/285-377-1), European Commission."},{"key":"ref_48","unstructured":"PubChem (2026, March 15). Essential Oils, Melaleuca alternifolia, Available online: https:\/\/pubchem.ncbi.nlm.nih.gov."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1318","DOI":"10.1016\/j.ijbiomac.2022.09.038","article-title":"Preparation and characterization of long-term antibacterial and pH-responsive polylactic acid\/octenyl succinic anhydride-chitosan @ tea tree oil microcapsules","volume":"220","author":"Zhu","year":"2022","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"106571","DOI":"10.1016\/j.fbio.2025.106571","article-title":"New frontiers in the exploration of phenolic compounds and other bioactives as natural preservatives","volume":"68","author":"Aquino","year":"2025","journal-title":"Food Biosci."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S1046-5928(02)00641-1","article-title":"Practical considerations in refolding proteins from inclusion bodies","volume":"28","author":"Tsumoto","year":"2003","journal-title":"Protein Expr. Purif."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Sadgrove, N.J., Padilla-Gonzalez, G.F., and Phumthum, M. (2022). Fundamental chemistry of essential oils and volatile organic compounds, methods of analysis and authentication. Plants, 11.","DOI":"10.3390\/plants11060789"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1016\/j.oraloncology.2005.10.002","article-title":"Susceptibility to Melaleuca alternifolia (Tea Tree) Oil of Yeasts Isolated from the Mouths of Patients with Advanced Cancer","volume":"42","author":"Bagg","year":"2006","journal-title":"Oral Oncol."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Becerril, R., Nerin, C., and Silva, F. (2020). Encapsulation systems for antimicrobial food packaging components: An update. Molecules, 25.","DOI":"10.3390\/molecules25051134"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1515\/reveh-2017-0023","article-title":"Antifungal properties of essential oils for improvement of indoor air quality: A review","volume":"33","author":"Whiley","year":"2017","journal-title":"Rev. Environ. Health"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.ces.2014.08.046","article-title":"Controlled Drug Release from Pharmaceutical Nanocarriers","volume":"125","author":"Lee","year":"2014","journal-title":"Chem. Eng. Sci."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1021\/tx200486f","article-title":"\u03b1-Terpinene, an Antioxidant in Tea Tree Oil, Autoxidizes Rapidly to Skin Allergens on Air Exposure","volume":"25","author":"Nilsson","year":"2012","journal-title":"Chem. Res. Toxicol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"e018507","DOI":"10.1136\/bmjopen-2017-018507","article-title":"Treatment of Scabies Using a Tea Tree Oil-Based Gel Formulation in Australian Aboriginal Children: Protocol for a Randomised Controlled Trial","volume":"8","author":"Thomas","year":"2018","journal-title":"BMJ Open"},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Hao, M., Tan, X., Liu, K., and Xin, N. (2026). Nanoencapsulation of Nutraceuticals: Enhancing Stability and Bioavailability in Functional Foods. Front. Nutr., 12.","DOI":"10.3389\/fnut.2025.1746176"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.crfs.2019.10.002","article-title":"Antioxidant Active Packaging Systems to Extend the Shelf Life of Sliced Cooked Ham","volume":"1","author":"Pateiro","year":"2019","journal-title":"Curr. Res. Food Sci."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"100025","DOI":"10.1016\/j.jfp.2022.100025","article-title":"A Review of Regulatory Standards and Advances in Essential Oils as Antimicrobials in Foods","volume":"86","author":"White","year":"2023","journal-title":"J. Food Prot."},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Moirangthem, S., Patra, G., Biswas, S., Das, A., Nath, S., Verma, A.K., Pal, S., Chatterjee, N., Bandyopadhyay, S., and Nanda, P.K. (2024). Effect of Nutmeg (Myristica fragrans) and Tea Tree (Melaleuca alternifolia) Essential Oils on the Oxidative and Microbial Stability of Chicken Fillets During Refrigerated Storage. Foods, 13.","DOI":"10.3390\/foods13244139"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1921","DOI":"10.1007\/s10068-024-01812-9","article-title":"Application of Tea Tree Oil Nanoemulsion Pads in Lateolabrax japonicas Fillets","volume":"34","author":"Feng","year":"2025","journal-title":"Food Sci. Biotechnol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"4581","DOI":"10.1021\/jm060228i","article-title":"Interaction of tea tree oil with model and cellular membranes","volume":"49","author":"Giordani","year":"2006","journal-title":"J. Med. Chem."},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Perricone, M., Arace, E., Corbo, M.R., Sinigaglia, M., and Bevilacqua, A. (2015). Bioactivity of essential oils: A review on their interaction with food components. Front. Microbiol., 6.","DOI":"10.3389\/fmicb.2015.00076"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"e28125","DOI":"10.1016\/j.heliyon.2024.e28125","article-title":"Quality criteria, chemical composition and antimicrobial activity of the essential oil of Mentha suaveolens Ehrh","volume":"10","author":"Afrokh","year":"2024","journal-title":"Heliyon"},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Zhang, Y.-L., You, C.-X., Li, Y.-Y., and Hao, Y.-J. (2020). Advances in Biosynthesis, Regulation, and Function of Apple Cuticular Wax. Front. Plant Sci., 11.","DOI":"10.3389\/fpls.2020.01165"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"102388","DOI":"10.1016\/j.fochx.2025.102388","article-title":"Protein and Polysaccharide Edible Coatings: A Promising Approach for Fruits Preservation\u2014Recent Advances","volume":"27","author":"Lieu","year":"2025","journal-title":"Food Chem. X"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/j.ijfoodmicro.2004.03.022","article-title":"Essential oils: Their antibacterial properties and potential applications in foods\u2014A review","volume":"94","author":"Burt","year":"2004","journal-title":"Int. J. Food Microbiol."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"127268","DOI":"10.1016\/j.foodchem.2020.127268","article-title":"Essential Oils: A Promising Eco-Friendly Food Preservative","volume":"330","author":"Falleh","year":"2020","journal-title":"Food Chem."},{"key":"ref_71","doi-asserted-by":"crossref","unstructured":"Maes, C., Bouquillon, S., and Fauconnier, M.-L. (2019). Encapsulation of Essential Oils for the Development of Biosourced Pesticides with Controlled Release: A Review. Molecules, 24.","DOI":"10.3390\/molecules24142539"},{"key":"ref_72","doi-asserted-by":"crossref","unstructured":"Borotov\u00e1, P., Galovi\u010dov\u00e1, L., Vukovic, N.L., Vukic, M., Tvrd\u00e1, E., and Ka\u010d\u00e1niov\u00e1, M. (2022). Chemical and Biological Characterization of Melaleuca alternifolia Essential Oil. Plants, 11.","DOI":"10.3390\/plants11040558"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"1100","DOI":"10.2174\/1389200219666180723144850","article-title":"Essential Oils: Extraction Techniques, Pharmaceutical and Therapeutic Potential\u2014A Review","volume":"19","author":"Aziz","year":"2018","journal-title":"Curr. Drug Metab."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"2325","DOI":"10.1039\/C9GC03878G","article-title":"A Review of Sustainable and Intensified Techniques for Extraction of Food and Natural Products","volume":"22","author":"Chemat","year":"2020","journal-title":"Green Chem."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"461770","DOI":"10.1016\/j.chroma.2020.461770","article-title":"Selective Extraction of Bioactive Compounds from Plants Using Recent Extraction Techniques: A Review","volume":"1635","author":"Lefebvre","year":"2021","journal-title":"J. Chromatogr. A"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"105325","DOI":"10.1016\/j.ultsonch.2020.105325","article-title":"Ultrasound Assisted Extraction (UAE) of Bioactive Compounds from Fruit and Vegetable Processing By-Products: A Review","volume":"70","author":"Kumar","year":"2021","journal-title":"Ultrason. Sonochem."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/j.chroma.2019.01.034","article-title":"Evaluation of a Liquid Electron Ionization Liquid Chromatography\u2013Mass Spectrometry Interface","volume":"1591","author":"Termopoli","year":"2019","journal-title":"J. Chromatogr. A"},{"key":"ref_78","doi-asserted-by":"crossref","unstructured":"Srivastav, P.P., Srivastava, B., and Karunanithi, S. (2025). Chemical Composition Analysis for Essential Oils by GC-MS. Essential Oil Extraction from Food By-Products; Methods and Protocols in Food Science, Humana.","DOI":"10.1007\/978-1-0716-4634-2"},{"key":"ref_79","unstructured":"Buffalo Extraction Systems (2026, March 15). Food Grade Essential Oils for Pharmaceutical and Nutraceutical Applications: Purity Benchmarks and Regulatory Hurdles. Buffalo Extraction Systems Knowledge Center. Available online: https:\/\/www.buffaloextracts.com\/knowledge\/food-grade-essential-oils-for-pharmaceutical-and-nutraceutical-applications-purity-benchmarks-and-regulatory-hurdles\/."},{"key":"ref_80","unstructured":"New Directions Aromatics (2020). An Easy-to-Understand Guide to GCMS Testing, New Directions Aromatics Blog. Available online: https:\/\/www.newdirectionsaromatics.com\/blog\/an-easy-to-understand-guide-to-gcms-testing."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"4817","DOI":"10.1021\/acs.jafc.6b01803","article-title":"Determination of Enantiomeric Distribution of Terpenes for Quality Assessment of Australian Tea Tree Oil","volume":"64","author":"Davies","year":"2016","journal-title":"J. Agric. Food Chem."},{"key":"ref_82","unstructured":"Chiralpedia (2025). Chiral Analysis: Mapping the Essentials, Chiralpedia Blog. Available online: https:\/\/chiralpedia.com\/blog\/chiral-analysis-mapping-the-essentials\/."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"12583","DOI":"10.1021\/acs.est.3c01624","article-title":"Comprehensive Two-Dimensional Gas Chromatography with Peak Tracking for Screening of Constituent Biodegradation in Petroleum UVCB Substances","volume":"57","author":"Booth","year":"2023","journal-title":"Environ. Sci. Technol."},{"key":"ref_84","doi-asserted-by":"crossref","unstructured":"Wang, L., Zhang, Y., Liu, L., Huang, F., and Dong, B. (2021). Effects of Three-Layer Encapsulated Tea Tree Oil on Growth Performance, Antioxidant Capacity, and Intestinal Microbiota of Weaned Pigs. Front. Vet. Sci., 8.","DOI":"10.3389\/fvets.2021.789225"},{"key":"ref_85","first-page":"42","article-title":"The Combination of ATR-FTIR and Chemometrics for Rapid Analysis of Essential Oil from Myrtaceae Plants\u2014A Review","volume":"12","author":"Ahmad","year":"2022","journal-title":"J. Appl. Pharm. Sci."},{"key":"ref_86","unstructured":"Spectroscopy (2026). Purity Analysis of Adulterated Essential Oils: Wintergreen, Tea Tree, Rosemary, and Lemon Eucalyptus Oil by FT-IR Spectroscopy, Spectroscopy Online. Available online: https:\/\/www.spectroscopyonline.com\/view\/purity-analysis-adulterated-essential-oils-wintergreen-tea-tree-rosemary-and-lemon-eucalyptus-oil-ft."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"137077","DOI":"10.1016\/j.foodchem.2023.137077","article-title":"Classification of Oils and Margarines by FTIR Spectroscopy in Tandem with Machine Learning","volume":"431","author":"Tachie","year":"2024","journal-title":"Food Chem."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/j.foodchem.2019.05.005","article-title":"Fast and Direct Analysis of Oxidation Levels of Oil-in-Water Emulsions Using ATR-FTIR","volume":"293","author":"Daoud","year":"2020","journal-title":"Food Chem."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"2009","DOI":"10.1007\/s11696-022-02603-4","article-title":"Through-container detection of tea tree oil adulteration using near-infrared spectroscopy (NIRS)","volume":"77","author":"Johnson","year":"2023","journal-title":"Chem. Pap."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"8276","DOI":"10.1021\/acs.jafc.1c02279","article-title":"Novel Strategy for the Recognition of Adulterant Vegetable Oils in Essential Oils Commonly Used in Food Industries by Applying 13C NMR Spectroscopy","volume":"69","author":"Truzzi","year":"2021","journal-title":"J. Agric. Food Chem."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1007\/s11746-006-1229-4","article-title":"The Importance of Peroxide Value in Assessing Food Quality and Food Safety","volume":"83","author":"Gotoh","year":"2006","journal-title":"J. Am. Oil Chem. Soc."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"462688","DOI":"10.1016\/j.chroma.2021.462688","article-title":"Fast and Versatile Analysis of Liposome Encapsulation Efficiency by nanoparticle Exclusion Chromatography","volume":"1662","author":"Bian","year":"2022","journal-title":"J. Chromatogr. A"},{"key":"ref_93","doi-asserted-by":"crossref","unstructured":"Ntzimani, A., and Tsironi, T. (2026). Balancing Functionality and Safety in Food Packaging Coatings. Foods, 15.","DOI":"10.3390\/foods15030571"},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.ijfoodmicro.2019.01.004","article-title":"Inhibition of Listeria monocytogenes by Melaleuca alternifolia (tea tree) essential oil in ground beef","volume":"293","author":"Stamford","year":"2019","journal-title":"Int. J. Food Microbiol."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"853","DOI":"10.1046\/j.1365-2672.2003.02059.x","article-title":"Antifungal activity of the components of Melaleuca alternifolia (tea tree) oil","volume":"95","author":"Hammer","year":"2003","journal-title":"J. Appl. Microbiol."},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1016\/j.micpath.2018.09.036","article-title":"The antimicrobial activities and action-mechanism of tea tree oil against food-borne bacteria in fresh cucumber juice","volume":"125","author":"Shi","year":"2018","journal-title":"Microb. Pathog."},{"key":"ref_97","doi-asserted-by":"crossref","unstructured":"Thompson, G., Blackwood, B., McMullan, R., Alderdice, F.A., Trinder, T.J., Lavery, G.G., and McAuley, D.F. (2008). A Randomized Controlled Trial of Tea Tree Oil (5%) Body Wash Versus Standard Body Wash to Prevent Colonization with Methicillin-Resistant Staphylococcus aureus (MRSA) in Critically Ill Adults: Research Protocol. BMC Infect. Dis., 8.","DOI":"10.1186\/1471-2334-8-161"},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1071\/HI06112","article-title":"Sporicidal Activity of Tea Tree Oil","volume":"11","author":"Messager","year":"2006","journal-title":"Aust. Infect. Control"},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"107745","DOI":"10.1016\/j.foodcont.2020.107745","article-title":"The Effect of Natural Antimicrobials Against Campylobacter spp. and Its Similarities to Salmonella spp., Listeria spp., Escherichia coli, Vibrio spp., Clostridium spp. and Staphylococcus spp","volume":"121","author":"Balta","year":"2021","journal-title":"Food Control"},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"794","DOI":"10.1016\/j.foodcont.2014.10.029","article-title":"Salmonella enterica and Listeria monocytogenes inactivation dynamics after treatment with selected essential oils","volume":"50","author":"Mazzarrino","year":"2015","journal-title":"Food Control"},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"102943","DOI":"10.1016\/j.fochx.2025.102943","article-title":"Harnessing Essential Oils for Sustainable Food Preservatives: Chemical Composition, Mechanisms, Applications, and Safety Insights","volume":"30","author":"Rais","year":"2025","journal-title":"Food Chem. X"},{"key":"ref_102","doi-asserted-by":"crossref","unstructured":"Dong, X., Bond, A.E., and Yang, L. (2019). Essential oil-incorporated carbon nanotubes filters for bacterial removal and inactivation. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0227220"},{"key":"ref_103","doi-asserted-by":"crossref","unstructured":"Wr\u00f3blewska, M., Szyma\u0144ska, E., and Winnicka, K. (2021). The influence of tea tree oil on antifungal activity and pharmaceutical characteristics of Pluronic\u00ae F-127 gel formulations with ketoconazole. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms222111326"},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"100846","DOI":"10.1016\/j.phyplu.2025.100846","article-title":"Australian tea tree (Melaleuca alternifolia) oil: An updated review of antimicrobial and other medicinal properties","volume":"5","author":"Bugarcic","year":"2025","journal-title":"Phytomed. Plus"},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"1239","DOI":"10.21474\/IJAR01\/9778","article-title":"Antifungal Activity of Tea Tree Essential Oils (Melaleuca alternifolia) Against Phytopathogenic Fungi","volume":"7","author":"Le","year":"2019","journal-title":"Int. J. Adv. Res."},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1093\/jac\/dkf112","article-title":"In vitro activity of Melaleuca alternifolia (tea tree) oil against dermatophytes and other filamentous fungi","volume":"50","author":"Hammer","year":"2002","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_107","doi-asserted-by":"crossref","unstructured":"Kursa, W., Jamio\u0142kowska, A., Wyrostek, J., and Kowalski, R. (2022). Antifungal Effect of Plant Extracts on the Growth of the Cereal Pathogen Fusarium spp.\u2014An In Vitro Study. Agronomy, 12.","DOI":"10.3390\/agronomy12123204"},{"key":"ref_108","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1016\/j.lwt.2018.06.026","article-title":"Fabrication of chitosan nanofibers containing tea tree oil liposomes against Salmonella spp. in chicken","volume":"96","author":"Cui","year":"2018","journal-title":"LWT"},{"key":"ref_109","unstructured":"Ponce, A., Ag\u00fcero, M.V., Roura, S.I., and Moreira, M.R. (September, January 31). Preharvest Soil Management Affects the Native Microflora and Quality of Butter Lettuce. Proceedings of the CIGR International Conference of Agricultural Engineering and XXXVII Congresso Brasileiro de Engenharia Agr\u00edcola (CONBEA 2008), Paran\u00e1, Brazil."},{"key":"ref_110","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1590\/S1517-83822010005000005","article-title":"Antimicrobial activity of essential oils against vancomycin-resistant enterococci (vre) and Escherichia coli O157:H7 in feta soft cheese and minced beef meat","volume":"42","author":"Selim","year":"2011","journal-title":"Braz. J. Microbiol."},{"key":"ref_111","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1016\/j.postharvbio.2012.11.010","article-title":"Effects and Possible Mechanisms of Tea Tree Oil Vapor Treatment on the Main Disease in Postharvest Strawberry Fruit","volume":"77","author":"Shao","year":"2013","journal-title":"Postharvest Biol. Technol."},{"key":"ref_112","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.ijbiomac.2022.05.164","article-title":"Development and Characterization of Sodium Alginate\/Tea Tree Essential Oil Nanoemulsion Active Film Containing TiO2 Nanoparticles for Banana Packaging","volume":"213","author":"Yang","year":"2022","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_113","doi-asserted-by":"crossref","first-page":"3075","DOI":"10.1111\/1750-3841.16666","article-title":"Electrospun Chitosan-Based Nanofibers Loading Tea Tree Oil for Fresh Salmon Fillet Shelf-Life Extension","volume":"88","author":"Xia","year":"2023","journal-title":"J. Food Sci."},{"key":"ref_114","doi-asserted-by":"crossref","unstructured":"Safakas, K., Lainioti, G.C., Tsiamis, G., Stathopoulou, P., and Ladavos, A. (2025). Utilizing Essential Oil Components as Natural Antifungal Preservatives in the Active Packaging of Bread. Polymers, 17.","DOI":"10.3390\/polym17050697"},{"key":"ref_115","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1167\/tvst.2.7.2","article-title":"Terpinen-4-ol is the most active ingredient of tea tree oil to kill Demodex mites","volume":"2","author":"Tighe","year":"2013","journal-title":"Transl. Vis. Sci. Technol."},{"key":"ref_116","doi-asserted-by":"crossref","first-page":"616","DOI":"10.1016\/j.fct.2005.09.001","article-title":"A review of the toxicity of Melaleuca alternifolia (tea tree) oil","volume":"44","author":"Hammer","year":"2006","journal-title":"Food Chem. Toxicol."},{"key":"ref_117","first-page":"343","article-title":"Antiviral activity of Australian tea tree oil and eucalyptus oil against herpes simplex virus in cell culture","volume":"56","author":"Schnitzler","year":"2001","journal-title":"Pharmazie"},{"key":"ref_118","doi-asserted-by":"crossref","first-page":"806","DOI":"10.1111\/j.1472-765X.2009.02740.x","article-title":"In vitro antiviral activity of Melaleuca alternifolia essential oil","volume":"49","author":"Garozzo","year":"2009","journal-title":"Lett. Appl. Microbiol."},{"key":"ref_119","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.virol.2016.04.021","article-title":"The in vitro and in vivo antiviral properties of combined monoterpene alcohols against West Nile virus infection","volume":"495","author":"Zamora","year":"2016","journal-title":"Virology"},{"key":"ref_120","doi-asserted-by":"crossref","first-page":"116356","DOI":"10.1016\/j.bmc.2021.116356","article-title":"Antiviral strategies targeting host factors and mechanisms obliging +ssRNA viral pathogens","volume":"46","author":"Mahajan","year":"2021","journal-title":"Bioorg. Med. Chem."},{"key":"ref_121","doi-asserted-by":"crossref","unstructured":"Li, Y., Liu, D., Wang, Y., Su, W., Liu, G., and Dong, W. (2021). The importance of glycans of viral and host proteins in enveloped virus infection. Front. Immunol., 12.","DOI":"10.3389\/fimmu.2021.571509"},{"key":"ref_122","doi-asserted-by":"crossref","unstructured":"Romeo, A., Iacovelli, F., Scagnolari, C., Scordio, M., Frasca, F., Cond\u00f2, R., Ammendola, S., Gaziano, R., Anselmi, M., and Divizia, M. (2022). Potential use of tea tree oil as a disinfectant agent against coronaviruses: A combined experimental and simulation study. Molecules, 27.","DOI":"10.3390\/molecules27123786"},{"key":"ref_123","doi-asserted-by":"crossref","first-page":"1295","DOI":"10.1080\/02786826.2012.708948","article-title":"Inactivation of airborne influenza virus by tea tree and eucalyptus oils","volume":"46","author":"Pyankov","year":"2012","journal-title":"Aerosol Sci. Technol."},{"key":"ref_124","doi-asserted-by":"crossref","first-page":"1914","DOI":"10.1128\/AAC.46.6.1914-1920.2002","article-title":"Mechanism of action of Melaleuca alternifolia (tea tree) oil on Staphylococcus aureus determined by time-kill, lysis, leakage, and salt tolerance assays and electron microscopy","volume":"46","author":"Carson","year":"2002","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_125","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1016\/S0167-7012(02)00248-8","article-title":"Leakage of K+ ions from Staphylococcus aureus in response to tea tree oil","volume":"53","author":"Hada","year":"2003","journal-title":"J. Microbiol. Methods"},{"key":"ref_126","doi-asserted-by":"crossref","unstructured":"Braz, V.S., Melchior, K., and Moreira, C.G. (2020). Escherichia coli as a multifaceted pathogenic and versatile bacterium. Front. Cell. Infect. Microbiol., 10.","DOI":"10.3389\/fcimb.2020.548492"},{"key":"ref_127","doi-asserted-by":"crossref","first-page":"2293","DOI":"10.1007\/s42770-024-01347-5","article-title":"Phenotypic and molecular characterization of clinically isolated antibiotics-resistant S. aureus (MRSA), E. coli (ESBL) and Acinetobacter 1379 bacterial strains","volume":"55","author":"Iram","year":"2024","journal-title":"Braz. J. Microbiol."},{"key":"ref_128","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1046\/j.1365-2672.2000.00943.x","article-title":"The mode of antimicrobial action of the essential oil of Melaleuca alternifolia (tea tree oil)","volume":"88","author":"Cox","year":"2000","journal-title":"J. Appl. Microbiol."},{"key":"ref_129","doi-asserted-by":"crossref","unstructured":"Didehdar, M., Chegini, Z., and Shariati, A. (2022). Eugenol: A novel therapeutic agent for the inhibition of Candida species infection. Front. Pharmacol., 13.","DOI":"10.3389\/fphar.2022.872127"},{"key":"ref_130","doi-asserted-by":"crossref","unstructured":"Zheng, D., Liu, J., Piao, H., Zhu, Z., Wei, R., and Li, K. (2022). ROS-Triggered Endothelial Cell Death Mechanisms: Focus on Pyroptosis, Parthanatos, and Ferroptosis. Front. Immunol., 13.","DOI":"10.3389\/fimmu.2022.1039241"},{"key":"ref_131","doi-asserted-by":"crossref","unstructured":"Huang, Y., Ebrahimi, H., Berselli, E., Foti, M.C., and Amorati, R. (2025). Essential Oils as Antioxidants: Mechanistic Insights from Radical Scavenging to Redox Signaling. Antioxidants, 15.","DOI":"10.3390\/antiox15010037"},{"key":"ref_132","doi-asserted-by":"crossref","first-page":"100082","DOI":"10.1016\/j.arres.2023.100082","article-title":"Terpinen-4-ol, A Volatile Terpene Molecule, Extensively Electrifies the Biological Systems Against the Oxidative Stress-Linked Pathogenesis","volume":"9","author":"Deen","year":"2023","journal-title":"Adv. Redox Res."},{"key":"ref_133","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1093\/jac\/dkh359","article-title":"Tolerance of Pseudomonas aeruginosa to Melaleuca alternifolia (Tea Tree) Oil Is Associated with the Outer Membrane and Energy-Dependent Cellular Processes","volume":"54","author":"Longbottom","year":"2004","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_134","doi-asserted-by":"crossref","first-page":"590470","DOI":"10.1155\/2015\/590470","article-title":"The influence of tea tree oil (Melaleuca alternifolia) on fluconazole activity against fluconazole-resistant Candida albicans strains","volume":"2015","author":"Mertas","year":"2015","journal-title":"BioMed Res. Int."},{"key":"ref_135","doi-asserted-by":"crossref","unstructured":"Noumi, E., Merghni, A., Alreshidi, M.M., Haddad, O., Akmadar, G., De Martino, L., Mastouri, M., Ceylan, O., Snoussi, M., and Al-Sieni, A. (2018). Chromobacterium violaceum and Pseudomonas aeruginosa PAO1: Models for evaluating anti-quorum sensing activity of Melaleuca alternifolia essential oil and its main component terpinen-4-ol. Molecules, 23.","DOI":"10.3390\/molecules23102672"},{"key":"ref_136","doi-asserted-by":"crossref","unstructured":"Cheng, F., Mo, Y., Chen, K., Shang, X., Yang, Z., Hao, B., Shang, R., Liang, J., and Liu, Y. (2021). Integration of Metabolomics and Transcriptomics Indicates Changes in MRSA Exposed to Terpinen-4-ol. BMC Microbiol., 21.","DOI":"10.1186\/s12866-021-02348-2"},{"key":"ref_137","doi-asserted-by":"crossref","unstructured":"Salinas, C., Florent\u00edn, G., Rodr\u00edguez, F., Alvarenga, N., and Guill\u00e9n, R. (2022). Terpenes Combinations Inhibit Biofilm Formation in Staphylococcus aureus by Interfering with Initial Adhesion. Microorganisms, 10.","DOI":"10.20944\/preprints202206.0232.v1"},{"key":"ref_138","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1016\/j.ijantimicag.2008.08.028","article-title":"Effects of Tea Tree (Melaleuca alternifolia) Oil on Staphylococcus aureus in Biofilms and Stationary Growth Phase","volume":"33","author":"Eick","year":"2009","journal-title":"Int. J. Antimicrob. Agents."},{"key":"ref_139","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1007\/s00284-018-1594-x","article-title":"In vitro antimicrobial activities of commercially available tea tree (Melaleuca alternifolia) essential oils","volume":"76","author":"Brun","year":"2019","journal-title":"Curr. Microbiol."},{"key":"ref_140","doi-asserted-by":"crossref","unstructured":"Iseppi, R., Di Cerbo, A., Aloisi, P., Manelli, M., Pellesi, V., Provenzano, C., Camellini, S., Messi, P., and Sabia, C. (2020). In vitro activity of essential oils against planktonic and biofilm cells of extended-spectrum \u03b2-lactamase (ESBL)\/carbapenamase-producing gram-negative bacteria involved in human nosocomial infections. Antibiotics, 9.","DOI":"10.3390\/antibiotics9050272"},{"key":"ref_141","doi-asserted-by":"crossref","unstructured":"Sagsoz, N.P., Orhan, F., Baris, O., and Sagsoz, O. (2025). In Vitro Evaluation of Plant Antimicrobials Against Candida albicans Biofilm on Denture Base Materials: A Comparison with Chemical Denture Cleansers. Polymers, 17.","DOI":"10.3390\/polym17212869"},{"key":"ref_142","doi-asserted-by":"crossref","first-page":"1261","DOI":"10.4315\/0362-028X.JFP-19-337","article-title":"Rosemary and tea tree essential oils exert antibiofilm activities in vitro against Staphylococcus aureus and Escherichia coli","volume":"83","author":"Liu","year":"2020","journal-title":"J. Food Prot."},{"key":"ref_143","doi-asserted-by":"crossref","first-page":"e062","DOI":"10.1590\/1807-3107bor-2019.vol33.0062","article-title":"Anti-biofilm and anti-inflammatory effect of a herbal nanoparticle mouthwash: A randomized crossover trial","volume":"33","author":"Casarin","year":"2019","journal-title":"Braz. Oral Res."},{"key":"ref_144","doi-asserted-by":"crossref","unstructured":"Cordeiro, L., Figueiredo, P., Souza, H., Sousa, A., Andrade-J\u00fanior, F., Medeiros, D., N\u00f3brega, J., Silva, D., Martins, E., and Barbosa-Filho, J. (2020). Terpinen-4-ol as an Antibacterial and Antibiofilm Agent Against Staphylococcus aureus. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21124531"},{"key":"ref_145","doi-asserted-by":"crossref","first-page":"e70896","DOI":"10.1111\/1750-3841.70896","article-title":"Multifunctional OEO-ZIF-8-HA nanoparticles for antibacterial control on latex surfaces and baby arugula (Eruca sativa) leaves","volume":"91","author":"Nguyen","year":"2026","journal-title":"J. Food Sci."},{"key":"ref_146","doi-asserted-by":"crossref","first-page":"136404","DOI":"10.1016\/j.ijbiomac.2024.136404","article-title":"A chitosan-modified tea tree oil self-nanoemulsion improves antibacterial effects in vivo and in vitro and promotes wound healing by influencing metabolic processes against multidrug-resistant bacterial infections","volume":"281","author":"Cai","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_147","doi-asserted-by":"crossref","unstructured":"de Campos Rasteiro, V.M., da Costa, A.C.B., Ara\u00fajo, C.F., de Barros, P.P., Rossoni, R.D., Anbinder, A.L., Jorge, A.O.C., and Junqueira, J.C. (2014). Essential oil of Melaleuca alternifolia for the treatment of oral candidiasis induced in an immunosuppressed mouse model. BMC Complement. Altern. Med., 14.","DOI":"10.1186\/1472-6882-14-489"},{"key":"ref_148","doi-asserted-by":"crossref","first-page":"838","DOI":"10.1248\/bpb.b13-00033","article-title":"Suppression of inflammatory reactions by terpinen-4-ol, a main constituent of tea tree oil, in a murine model of oral candidiasis and its suppressive activity to cytokine production of macrophages in vitro","volume":"36","author":"Ninomiya","year":"2013","journal-title":"Biol. Pharm. Bull."},{"key":"ref_149","first-page":"e12872","article-title":"Essential oils and their active components applied as: Free, encapsulated and in hurdle technology to fight microbial contaminations: A review","volume":"9","author":"Andrade","year":"2023","journal-title":"Heliyon"},{"key":"ref_150","doi-asserted-by":"crossref","first-page":"113260","DOI":"10.1016\/j.postharvbio.2024.113260","article-title":"Recent advances in the application of tea tree oil in the storage of fruit and vegetables","volume":"219","author":"Deng","year":"2025","journal-title":"Postharvest Biol. Technol."},{"key":"ref_151","doi-asserted-by":"crossref","unstructured":"Johansen, B., Duval, R.E., and Sergere, J.-C. (2022). First evidence of a combination of terpinen-4-ol and \u03b1-terpineol as a promising tool against ESKAPE pathogens. Molecules, 27.","DOI":"10.3390\/molecules27217472"},{"key":"ref_152","unstructured":"U.S. Food and Drug Administration (2019). Safety and effectiveness of consumer antiseptic rubs; topical antimicrobial drug products for over-the-counter human use. Fed. Regist., 84, 14847\u201314864."},{"key":"ref_153","doi-asserted-by":"crossref","unstructured":"Stef, L., Corcionivoschi, N., Julean, C., Callaway, T., Simiz, E., Marcu, A., Stef, D.S., Pet, I., Popescu, I., and Balta, I. (2025). Integrative Feeding Strategies with Essential Oils and Probiotics to Improve Raw Meat Quality and Carcass Traits in Broiler Chickens. Agriculture, 15.","DOI":"10.3390\/agriculture15222356"},{"key":"ref_154","doi-asserted-by":"crossref","first-page":"142007","DOI":"10.1016\/j.foodchem.2024.142007","article-title":"Study examines the use of tea tree oil in fruit and vegetable preservation","volume":"465","author":"Liu","year":"2025","journal-title":"Food Chem."},{"key":"ref_155","doi-asserted-by":"crossref","unstructured":"Yang, T., Feng, F., Zhan, K., Ma, X., Jiang, M., Datsomor, O., Zhu, X., Huo, Y., and Zhao, G. (2022). Effect of the Tea Tree Oil on Growth Performance, Meat Quality, Serum Biochemical Indices, and Antioxidant Capacity in Finishing Pigs. Front. Vet. Sci., 9.","DOI":"10.3389\/fvets.2022.916625"},{"key":"ref_156","doi-asserted-by":"crossref","first-page":"6282","DOI":"10.3168\/jds.2012-5478","article-title":"Evaluation of Antimicrobial Edible Coatings from a Whey Protein Isolate Base to Improve the Shelf Life of Cheese","volume":"95","author":"Ramos","year":"2012","journal-title":"J. Dairy Sci."},{"key":"ref_157","doi-asserted-by":"crossref","first-page":"17181","DOI":"10.1111\/jfpp.17181","article-title":"Fabrication the antimicrobial sachet by encapsulation of peppermint essential oil in active packaging of strawberry fruit","volume":"46","author":"Amiri","year":"2022","journal-title":"J. Food Process. Preserv."},{"key":"ref_158","doi-asserted-by":"crossref","first-page":"4820","DOI":"10.1007\/s11694-024-02536-4","article-title":"Evaluation of the postharvest quality of Agaricus bisporus packed using PVA\/SG-based active packaging film containing tea tree essential oil","volume":"18","author":"Wang","year":"2024","journal-title":"Food Meas."},{"key":"ref_159","doi-asserted-by":"crossref","unstructured":"Soutelino, M.E.M., Silva, A.C.O., and Rocha, R.S. (2024). Natural Antimicrobials in Dairy Products: Benefits, Challenges, and Future Trends. Antibiotics, 13.","DOI":"10.3390\/antibiotics13050415"},{"key":"ref_160","doi-asserted-by":"crossref","first-page":"118090","DOI":"10.1016\/j.lwt.2025.118090","article-title":"Enhancing shelf life of refrigerated salmon: Synergistic roles of glutaric and azelaic acid with conventional preservatives","volume":"228","author":"Liao","year":"2025","journal-title":"LWT"},{"key":"ref_161","first-page":"33","article-title":"Comparison of the effect of nanoemulsion and emulsion coating containing of Oliveria decumbens essential oil on bacteria inoculated into rainbow trout fillet","volume":"16","author":"Leila","year":"2024","journal-title":"Electron. J. Food Process. Preserv."},{"key":"ref_162","doi-asserted-by":"crossref","unstructured":"Lai, H., Chen, S., Su, X., Huang, X., Zheng, Q., Yang, M., Shen, B., and Yue, P. (2023). Sponge-liked silica nanoporous particles for sustaining release and long-term antibacterial activity of natural essential oil. Molecules, 28.","DOI":"10.3390\/molecules28020594"},{"key":"ref_163","doi-asserted-by":"crossref","first-page":"710","DOI":"10.3390\/ma11050710","article-title":"Preparation of tea tree oil\/poly(styrene-butyl methacrylate) microspheres with sustained release and anti-bacterial properties","volume":"11","author":"Lin","year":"2018","journal-title":"Materials"},{"key":"ref_164","doi-asserted-by":"crossref","first-page":"133357","DOI":"10.1016\/j.ijbiomac.2024.133357","article-title":"Organic nanoparticles incorporated starch\/carboxymethylcellulose multifunctional coating film for efficient preservation of perishable products","volume":"275","author":"Li","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_165","doi-asserted-by":"crossref","unstructured":"Kra\u015bniewska, K., and Gniewosz, M. (2025). Active Packaging Based on a PET\/PP Food-Grade Film Coated with Pullulan and Clove Essential Oil: Physicochemical and Antimicrobial Properties. Molecules, 30.","DOI":"10.3390\/molecules30102118"},{"key":"ref_166","doi-asserted-by":"crossref","unstructured":"Wu, T., Wang, M., Wang, P., Tian, H., and Zhan, P. (2022). Advances in the Formation and Control Methods of Undesirable Flavors in Fish. Foods, 11.","DOI":"10.3390\/foods11162504"},{"key":"ref_167","first-page":"104641","article-title":"Use of Melaleuca alternifolia Essential Oil as an Efficient Strategy to Extend the Shelf Life of Banana Fruits","volume":"54","author":"Ribeiro","year":"2023","journal-title":"Biochem. Syst. Ecol."},{"key":"ref_168","doi-asserted-by":"crossref","unstructured":"Dziagwa-Becker, M., and Oleszek, M. (2024). Is the biopesticide from tea tree oil an effective and low-risk alternative to chemical pesticides? A critical review. Molecules, 29.","DOI":"10.20944\/preprints202405.1765.v1"},{"key":"ref_169","first-page":"e182","article-title":"Fungal decay and shelf life of oranges coated with chitosan and bergamot, thyme, and tea tree essential oils","volume":"77","author":"Chiralt","year":"2012","journal-title":"J. Food Sci."},{"key":"ref_170","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.postharvbio.2017.11.008","article-title":"The combined effects of tea tree oil and hot air treatment on the quality and sensory characteristics and decay of strawberry","volume":"136","author":"Wei","year":"2018","journal-title":"Postharvest Biol. Technol."},{"key":"ref_171","doi-asserted-by":"crossref","unstructured":"Guan, Y., Lu, S., Sun, Y., Zhang, R., Lu, X., Pang, L., and Wang, L. (2024). Effect of tea tree essential oil on the quality, antioxidant activity, and microbiological safety of lightly processed lily (Lilium brownii var. viridulum) during storage. Foods, 13.","DOI":"10.3390\/foods13132106"},{"key":"ref_172","doi-asserted-by":"crossref","first-page":"135898","DOI":"10.1016\/j.foodchem.2023.135898","article-title":"Effect of sustained-release tea tree essential oil solid preservative on fresh-cut pineapple storage quality in modified atmospheres packaging","volume":"417","author":"Tian","year":"2023","journal-title":"Food Chem."},{"key":"ref_173","doi-asserted-by":"crossref","first-page":"112367","DOI":"10.1016\/j.foodcont.2026.112367","article-title":"Tea Tree Essential Oil as a Natural Food Preservative: Advances in Extraction, Bioactivity, and Encapsulation Strategies","volume":"190","author":"Qin","year":"2026","journal-title":"Food Control"},{"key":"ref_174","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1002\/ffj.3731","article-title":"Preparation of Tea Tree Oil Nanoemulsion: Characterisation, Antibacterial Mechanism and Evaluation of Apoptosis","volume":"38","author":"Han","year":"2023","journal-title":"Flavour. Fragr. J."},{"key":"ref_175","doi-asserted-by":"crossref","unstructured":"Napi\u00f3rkowska, A., Khaneghah, A.M., and Kurek, M.A. (2024). Essential Oil Nanoemulsions\u2014A New Strategy to Extend the Shelf Life of Smoothies. Foods, 13.","DOI":"10.3390\/foods13121854"},{"key":"ref_176","unstructured":"Red and Honey (2026, March 15). 13 Incredibly Practical Ways to Use Tea Tree Oil (It\u2019s Time It Became Your Favourite EO). Available online: https:\/\/redandhoney.com\/13-incredibly-practical-ways-use-tea-tree-oil-time-favourite-eo."},{"key":"ref_177","doi-asserted-by":"crossref","first-page":"100066","DOI":"10.1016\/j.onano.2022.100066","article-title":"Preparation, Stability and Biological Activity of Essential Oil-Based Nano Emulsions: A Comprehensive Review","volume":"8","author":"Singh","year":"2022","journal-title":"OpenNano"},{"key":"ref_178","doi-asserted-by":"crossref","unstructured":"Pereira, A.G., Perez-Vazquez, A., Barciela, P., Jorge, A.O.S., Yuksek, E.N., Nogueira-Marques, R., Seyyedi-Mansour, S., and Prieto, M.A. (2026). Bioactive Nanoemulsions for Enhancing Sausage and Meat Patty Shelf-Life. Foods, 15.","DOI":"10.3390\/foods15030430"},{"key":"ref_179","doi-asserted-by":"crossref","unstructured":"Han, Y.-L., Chen, C., Wu, Y.-Y., Yan, Y.-M., Wang, M.-Y., Xiang, Y., Wei, D.-Y., Hu, Z.-H., Zhuang, J., and Wang, Y.-H. (2025). Preparation of Tea Tree Oil Microcapsules and Their Effects on Strawberry Preservation During Storage. Horticulturae, 11.","DOI":"10.3390\/horticulturae11040389"},{"key":"ref_180","doi-asserted-by":"crossref","first-page":"899","DOI":"10.17576\/jsm-2025-5403-21","article-title":"Cellulose Nanocrystals and Zinc Oxide in Pineapple Starch Films for Enhanced Banana Shelf-Life","volume":"54","author":"Li","year":"2025","journal-title":"Sains Malays."},{"key":"ref_181","doi-asserted-by":"crossref","unstructured":"Sanchez-Navarro, M.M., Paya Nohales, F., Aran Ais, F., and Orgiles Barcelo, C. (2012). Polymer Shell Nanocapsules Containing a Natural Antimicrobial Oil for Footwear Applications. Proceedings of the UK Colloids 2011, Springer. Progress in Colloid and Polymer Science.","DOI":"10.1007\/978-3-642-28974-3_13"},{"key":"ref_182","doi-asserted-by":"crossref","first-page":"2102","DOI":"10.1016\/j.foodhyd.2009.05.006","article-title":"Characterization of Edible Films Based on Hydroxypropylmethylcellulose and Tea Tree Essential Oil","volume":"23","author":"Vargas","year":"2009","journal-title":"Food Hydrocoll."},{"key":"ref_183","doi-asserted-by":"crossref","first-page":"38","DOI":"10.22259\/2637-5583.0802004","article-title":"Chitosan as a Natural antimicrobial and Nanocarrier: Strategies for Food Preservation and surface Protection","volume":"8","author":"Nguyen","year":"2025","journal-title":"Res. J. Food Nutr."},{"key":"ref_184","doi-asserted-by":"crossref","unstructured":"Zhou, H., Li, J., Yu, C., Hu, M., Zhong, B., Tu, Z., and Peng, B. (2025). Gelatin\u2013Tea Tree Essential Oil Coating Improves the Quality, Flavor, and Micromolecular Metabolites of Squid (Illex argentinus) Muscle During Cold Storage. Foods, 14.","DOI":"10.3390\/foods14071160"},{"key":"ref_185","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1016\/j.foodcont.2015.02.008","article-title":"A Novel Active Packaging for Extending the Shelf-Life of Fresh Mushrooms (Agaricus bisporus)","volume":"54","author":"Wrona","year":"2015","journal-title":"Food Control"},{"key":"ref_186","first-page":"28","article-title":"Testing Antimicrobial Effects of Tea Tree Oil Using Saccharomyces cerevisiae and Bread Mold","volume":"7","author":"Patel","year":"2022","journal-title":"Can. J. Undergrad. Res."},{"key":"ref_187","doi-asserted-by":"crossref","unstructured":"Andrade, M.A., Barbosa, C.H., Ribeiro-Santos, R., Tom\u00e9, S., Fernando, A.L., Sanches Silva, A., and Vilarinho, F. (2025). Emerging Trends in Active Packaging for Food: A Six-Year Review. Foods, 14.","DOI":"10.3390\/foods14152713"},{"key":"ref_188","first-page":"353","article-title":"Effect of Preharvest Application of Chitosan and Tea Tree Essential Oil on Postharvest Evolution of Lettuce Native Microflora and Exogenous Escherichia coli O157:H7","volume":"34","author":"Tomadoni","year":"2014","journal-title":"J. Food Sci."},{"key":"ref_189","doi-asserted-by":"crossref","unstructured":"Micciche, A., Rothrock, M.J., Yang, Y., and Ricke, S.C. (2019). Essential oils as an intervention strategy to reduce Campylobacter in poultry production: A review. Front. Microbiol., 10.","DOI":"10.3389\/fmicb.2019.01058"},{"key":"ref_190","doi-asserted-by":"crossref","first-page":"1085","DOI":"10.1007\/s10787-024-01631-8","article-title":"Herbal Remedies for Oral and Dental Health: A Comprehensive Review of Their Multifaceted Mechanisms Including Antimicrobial, Anti-Inflammatory, and Antioxidant Pathways","volume":"33","author":"Anwar","year":"2025","journal-title":"Inflammopharmacology"},{"key":"ref_191","doi-asserted-by":"crossref","first-page":"59","DOI":"10.11138\/orl\/2017.10.1.059","article-title":"A Comparative Study of Antibacterial and Anti-Inflammatory Effects of Mouthrinse Containing Tea Tree Oil","volume":"10","author":"Salvatori","year":"2017","journal-title":"ORAL Implantol."},{"key":"ref_192","unstructured":"National Center for Complementary and Integrative Health (NCCIH) (2026, March 15). Tea Tree Oil: Usefulness and Safety, Available online: https:\/\/nccih.nih.gov\/health\/tea-tree-oil."},{"key":"ref_193","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1007\/s10787-024-01588-8","article-title":"Therapeutic Applications of Eucalyptus Essential Oils","volume":"33","author":"Atwa","year":"2025","journal-title":"Inflammopharmacology"},{"key":"#cr-split#-ref_194.1","unstructured":"European Commission (2009). Commission Regulation"},{"key":"#cr-split#-ref_194.2","unstructured":"(EC) No 450\/2009 of 29 May 2009 on active and intelligent materials and articles intended to come into contact with food. Off. J. Eur. Union, L135, 3-11. Available online: https:\/\/eur-lex.europa.eu\/LexUriServ\/LexUriServ.do?uri=OJ:L:2009:135:0003:0011:EN:PDF."},{"key":"ref_195","doi-asserted-by":"crossref","unstructured":"Nguyen, H.L., and Nguyen, T.B.N. (2026). Evaluation of Metal-Doped ZIF-8-Hyaluronic Acid Nanocomposites for Disruption of Salmonella Typhimurium and Escherichia coli on Food Contact (Stainless Steel) Surfaces. Sustainability, 18.","DOI":"10.3390\/su18062792"},{"key":"ref_196","doi-asserted-by":"crossref","first-page":"444","DOI":"10.4103\/0972-124X.118314","article-title":"Effect of Local Application of Tea Tree (Melaleuca alternifolia) Oil Gel on Long Pentraxin Level Used as an Adjunctive Treatment of Chronic Periodontitis: A Randomized Controlled Clinical Study","volume":"17","author":"Elgendy","year":"2013","journal-title":"J. Indian Soc. Periodontol."},{"key":"ref_197","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1055\/s-0040-1703999","article-title":"Tea tree oil versus chlorhexidine mouthwash in treatment of gingivitis: A pilot randomized, double blinded clinical trial","volume":"14","author":"Ripari","year":"2020","journal-title":"Eur. J. Dent."},{"key":"ref_198","unstructured":"Colgate (2026, March 15). Tea Tree Oil & Your Teeth. Colgate Oral Care Center. Available online: https:\/\/colgate.com\/en-us\/oral-health\/nutrition-and-oral-health\/tea-tree-oil-is-good-for-teeth."},{"key":"ref_199","unstructured":"ANSES (French Agency for Food, Environmental and Occupational Health & Safety) (2020). Food Supplements Containing Tea Tree, Niaouli and Cajeput Essential Oils: Their Misuse Can Pose Risks, ANSES. Available online: https:\/\/anses.fr\/en\/content\/food-supplements-containing-tea-tree-niaouli-and-cajeput-essential-oils-their-misuse-can."},{"key":"ref_200","doi-asserted-by":"crossref","unstructured":"Hyldgaard, M., Mygind, T., and Meyer, R.L. (2012). Essential oils in food preservation: Mode of action, synergies, and interactions with food matrix components. Front. Microbiol., 3.","DOI":"10.3389\/fmicb.2012.00012"},{"key":"ref_201","doi-asserted-by":"crossref","first-page":"1309","DOI":"10.1002\/ptr.7687","article-title":"Safety assessment and adverse drug reaction reporting of tea tree oil (Melaleuca aetheroleum)","volume":"37","author":"Bekhof","year":"2023","journal-title":"Phytother. Res."},{"key":"ref_202","doi-asserted-by":"crossref","unstructured":"Di Nunzio, M., Valli, V., Tom\u00e1s-Cobos, L., Tom\u00e1s-Chisbert, T., Murgui-Bosch, L., Danesi, F., and Bordoni, A. (2017). Is cytotoxicity a determinant of the different in vitro and in vivo effects of bioactives?. BMC Complement. Altern. Med., 17.","DOI":"10.1186\/s12906-017-1962-2"},{"key":"ref_203","doi-asserted-by":"crossref","first-page":"1355","DOI":"10.1016\/j.fct.2006.02.014","article-title":"The Toxic Effects of 4-tert-Octylphenol on the Reproductive System of Male Rats","volume":"44","author":"Bian","year":"2006","journal-title":"Food Chem. Toxicol."},{"key":"ref_204","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.meatsci.2007.11.014","article-title":"The Antimicrobial Effect of Thyme Essential Oil, Nisin and Their Combination Against Escherichia coli O157:H7 in Minced Beef During Refrigerated Storage","volume":"80","author":"Solomakos","year":"2008","journal-title":"Meat Sci."},{"key":"#cr-split#-ref_205.1","unstructured":"European Commission (2025). Commission Implementing Regulation"},{"key":"#cr-split#-ref_205.2","unstructured":"(EU) 2025\/1402 of 16 July 2025 Concerning the Authorisation of Tea Tree Essential Oil from Melaleuca alternifolia (Maiden & Betche) Cheel as a Feed Additive for All Animal Species. Official Journal of the European Union, European Commission. L 2025\/1402."},{"key":"ref_206","doi-asserted-by":"crossref","first-page":"1230","DOI":"10.1016\/j.atmosenv.2006.09.044","article-title":"The Effects of Evaporating Essential Oils on Indoor Air Quality","volume":"41","author":"Su","year":"2007","journal-title":"Atmos. Environ."},{"key":"ref_207","doi-asserted-by":"crossref","first-page":"117863","DOI":"10.1016\/j.atmosenv.2020.117863","article-title":"Indoor Use of Essential Oils: Emission Rates, Exposure Time and Impact on Air Quality","volume":"244","author":"Verriele","year":"2021","journal-title":"Atmos. Environ."},{"key":"ref_208","doi-asserted-by":"crossref","unstructured":"Louren\u00e7o, S.C., Mold\u00e3o-Martins, M., and Alves, V.D. (2019). Antioxidants of Natural Plant Origins: From Sources to Food Industry Applications. Molecules, 24.","DOI":"10.3390\/molecules24224132"},{"key":"ref_209","doi-asserted-by":"crossref","first-page":"1300","DOI":"10.4315\/JFP-22-017","article-title":"A Review of Essential Oils as Antimicrobials in Foods with Special Emphasis on Fresh Produce","volume":"85","author":"Gurtler","year":"2022","journal-title":"J. Food Prot."},{"key":"ref_210","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.ejps.2007.05.109","article-title":"Effect of a High-Fat Meal on Absorption and Disposition of Lipophilic Compounds: The Importance of Degree of Association with Triglyceride-Rich Lipoproteins","volume":"32","author":"Gershkovich","year":"2007","journal-title":"Eur. J. Pharm. Sci."},{"key":"ref_211","doi-asserted-by":"crossref","unstructured":"Kapetanakou, A.E., Mistriotis, A., Bozinaki, D.C., Tserotas, P., Athanasoulia, I.-G., Briassoulis, D., and Skandamis, P.N. (2024). Developing an Active Biodegradable Bio-Based Equilibrium Modified Atmosphere Packaging Containing a Carvacrol-Emitting Sachet for Cherry Tomatoes. Foods, 13.","DOI":"10.3390\/foods13213371"},{"key":"ref_212","doi-asserted-by":"crossref","first-page":"4934","DOI":"10.1039\/D3GC02647G","article-title":"Sustainable Biodegradable Coatings for Food Packaging: Challenges and Opportunities","volume":"26","author":"Jahangiri","year":"2024","journal-title":"Green Chem."},{"key":"ref_213","doi-asserted-by":"crossref","first-page":"20503121241250184","DOI":"10.1177\/20503121241250184","article-title":"Food Adulteration: Causes, Risks, and Detection Techniques\u2014Review","volume":"12","author":"Anagaw","year":"2024","journal-title":"SAGE Open Med."},{"key":"ref_214","doi-asserted-by":"crossref","first-page":"101222","DOI":"10.1016\/j.crfs.2025.101222","article-title":"Antioxidant Packaging Films: Application for Sustainable Food Protection","volume":"11","author":"Sani","year":"2025","journal-title":"Curr. Res. Food Sci."},{"key":"ref_215","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.tifs.2019.08.005","article-title":"Application of essential oil as a sustained release preparation in food packaging","volume":"92","author":"Ju","year":"2019","journal-title":"Trends Food Sci. Technol."},{"key":"ref_216","doi-asserted-by":"crossref","first-page":"101037","DOI":"10.1016\/j.fbio.2021.101037","article-title":"Composite chitosan films prepared using nisin and Perilla frutescense essential oil and their use to extend strawberry shelf life","volume":"41","author":"Wang","year":"2021","journal-title":"Food Biosci."},{"key":"ref_217","doi-asserted-by":"crossref","first-page":"e16933","DOI":"10.1111\/jfpp.16933","article-title":"Essential oil-based active polymer-based packaging system: A review of its effect on the antimicrobial, antioxidant, and sensory properties of beef and chicken meat","volume":"46","author":"Chacha","year":"2022","journal-title":"J. Food Process. Preserv."},{"key":"ref_218","doi-asserted-by":"crossref","unstructured":"Lopez-Gomez, A., Ros-Chumillas, M., Buendia-Moreno, L., and Martinez-Hernandez, G.B. (2020). Active cardboard packaging with encapsulated essential oils for enhancing the shelf life of fruit and vegetables. Front. Nutr., 7.","DOI":"10.3389\/fnut.2020.559978"},{"key":"ref_219","doi-asserted-by":"crossref","first-page":"102238","DOI":"10.1016\/j.psj.2022.102238","article-title":"Tea Tree Oil Nanoliposomes: Optimization, Characterization, and Antibacterial Activity Against Escherichia coli In Vitro and In Vivo","volume":"102","author":"Bo","year":"2023","journal-title":"Poult. Sci."},{"key":"ref_220","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1002\/ffj.70055","article-title":"Synergistic Essential Oil Blends: Chemical Composition, Antimicrobial Activity and Antioxidant Properties for Cosmetic Preservation","volume":"41","author":"Rekha","year":"2026","journal-title":"Flavour. Fragr. J."},{"key":"ref_221","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1111\/lam.12666","article-title":"Inhibition Effect of Tea Tree Oil on Listeria monocytogenes Growth and Exotoxin Proteins Listeriolysin O and p60 Secretion","volume":"63","author":"Liu","year":"2016","journal-title":"Lett. Appl. Microbiol."},{"key":"ref_222","doi-asserted-by":"crossref","first-page":"111593","DOI":"10.1016\/j.lwt.2021.111593","article-title":"The Combined Effect of Essential Oils and Emerging Technologies on Food Safety and Quality","volume":"147","author":"Pedrosa","year":"2021","journal-title":"LWT"},{"key":"ref_223","doi-asserted-by":"crossref","first-page":"101500","DOI":"10.1016\/j.fpsl.2025.101500","article-title":"Active packaging containing natural antimicrobials as a potential and innovative technology to extend shelf-life of fish products\u2014A review","volume":"49","author":"Kamau","year":"2025","journal-title":"Food Packag. Shelf Life"},{"key":"ref_224","doi-asserted-by":"crossref","first-page":"101510","DOI":"10.1016\/j.fochx.2024.101510","article-title":"Preparation and Characterization of Tea Tree Essential Oil Microcapsule-Coated Packaging Paper with Bacteriostatic Effect","volume":"23","author":"Zhu","year":"2024","journal-title":"Food Chem. X"},{"key":"ref_225","doi-asserted-by":"crossref","first-page":"103956","DOI":"10.1016\/j.fochx.2026.103956","article-title":"An Intelligent Delivery System for Apricot Preservation: pH-Responsive Hollow ZIF-8 Loaded with Lavender Essential Oil and Its Preservation Efficacy","volume":"36","author":"She","year":"2026","journal-title":"Food Chem. X"},{"key":"ref_226","doi-asserted-by":"crossref","first-page":"121348","DOI":"10.1016\/j.indcrop.2025.121348","article-title":"Supercritical CO2 Technology for Biomass Extraction: Review","volume":"233","author":"Nozari","year":"2025","journal-title":"Ind. Crops Prod."},{"key":"ref_227","doi-asserted-by":"crossref","unstructured":"Tarach, I., Olewnik-Kruszkowska, E., Richert, A., Gierszewska, M., and Rudawska, A. (2020). Influence of tea tree essential oil and poly(ethylene glycol) on antibacterial and physicochemical properties of polylactide-based films. Materials, 13.","DOI":"10.3390\/ma13214953"},{"key":"ref_228","doi-asserted-by":"crossref","first-page":"111114","DOI":"10.1016\/j.postharvbio.2019.111114","article-title":"Optimized preparation of tea tree oil complexation and their antifungal activity against Botrytis cinerea","volume":"162","author":"Yue","year":"2020","journal-title":"Postharvest Biol. Technol."},{"key":"ref_229","doi-asserted-by":"crossref","first-page":"112603","DOI":"10.1016\/j.fct.2021.112603","article-title":"Toxicological safety assessment of essential oils used as food supplements to establish safe oral recommended doses","volume":"157","author":"Tamburlin","year":"2026","journal-title":"Food Chem. Toxicol."},{"key":"ref_230","unstructured":"U.S. Food & Drug Administration (2009). Guidance for Industry: Recommendations for Submission of Chemical and Technological Data for Direct Food Additive Petitions, FDA. FDA-2020-D-1922."},{"key":"ref_231","doi-asserted-by":"crossref","unstructured":"Nguyen, H.L., Nguyen, H.M.X., and Nguyen, T.B.N. (2026). Data-driven engineering of antimicrobial nanomaterials for food safety and biomedical systems. Nanomaterials, 16.","DOI":"10.3390\/nano16120764"}],"container-title":["Materials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1996-1944\/19\/13\/2915\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T10:21:46Z","timestamp":1783419706000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1996-1944\/19\/13\/2915"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,7]]},"references-count":233,"journal-issue":{"issue":"13","published-online":{"date-parts":[[2026,7]]}},"alternative-id":["ma19132915"],"URL":"https:\/\/doi.org\/10.3390\/ma19132915","relation":{},"ISSN":["1996-1944"],"issn-type":[{"value":"1996-1944","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,7]]}}}