{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T13:14:02Z","timestamp":1788527642830,"version":"build-2803163510"},"reference-count":50,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T00:00:00Z","timestamp":1788480000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Materials"],"abstract":"<jats:p>Poly(L-lactide) (PLLA) scaffolds are widely used in tissue engineering because they are biodegradable and have good mechanical properties. Their performance changes during degradation, which is important for the design of biodegradable implants. In this study, we investigated the structural and mechanical stability of 3D-printed PLLA scaffolds during 180 days of in vitro hydrolytic degradation in phosphate-buffered saline (PBS) at 37 \u00b0C. Mechanical properties of the material were evaluated by tensile and compression tests. The material during degradation was analyzed for the scaffolds for which the lost mass and chemical and surface changes were analyzed using FTIR-ATR spectroscopy and SEM microscopy. The scaffolds lost more than 8% of their initial mass throughout the study, indicating slow degradation. However, their mechanical properties gradually decreased. Young\u2019s modulus dropped from 2.46 GPa to 2.09 GPa, and tensile strength decreased from 45.5 MPa to 31.1 MPa. The material also became more brittle after about 90 days. FTIR results confirmed progressive hydrolysis of ester bonds, while SEM images showed increasing surface roughness, micropores, and cracks. The results show that PLLA scaffolds maintain their shape and mass during the early stages of degradation. However, their mechanical strength decreases over time. These changes should be considered when designing biodegradable scaffolds for regenerative medicine.<\/jats:p>","DOI":"10.3390\/ma19173759","type":"journal-article","created":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T12:20:19Z","timestamp":1788524419000},"page":"3759","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Structural and Mechanical Durability Evaluation of PLLA Scaffolds for Biomedical Applications"],"prefix":"10.3390","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2692-5311","authenticated-orcid":false,"given":"Jagoda","family":"Kurowiak","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering, Institute of Material and Biomedical Engineering, Faculty of Engineering and Technology, University of Zielona Gora, Licealna 9 Street, 65-417 Zielona Gora, Poland"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"}]},{"given":"Aleksandra","family":"\u0141ysiak","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Institute of Material and Biomedical Engineering, Faculty of Engineering and Technology, University of Zielona Gora, Licealna 9 Street, 65-417 Zielona Gora, Poland"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2699-3528","authenticated-orcid":false,"given":"Tomasz","family":"Klekiel","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Institute of Material and Biomedical Engineering, Faculty of Engineering and Technology, University of Zielona Gora, Licealna 9 Street, 65-417 Zielona Gora, Poland"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"software","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Farjaminejad, R., Farjaminejad, S., Garcia-Godoy, F., Marya, A., Nucci, L., and Jamilian, A. (2026). Advances and Challenges in Tissue Engineering: Biomaterials, Cellular Strategies, and Clinical Applications. J. Funct. Biomater., 17.","DOI":"10.3390\/jfb17040184"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/S1369-7021(11)70058-X","article-title":"Biomaterials & scaffolds for tissue engineering","volume":"14","year":"2011","journal-title":"Mater. Today"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Socci, M.C., Rodr\u00edguez, G., Oliva, E., Fushimi, S., Takabatake, K., Nagatsuka, H., Felice, C.J., and Rodr\u00edguez, A.P. (2023). Polymeric Materials, Advances and Applications in Tissue Engineering: A Review. Bioengineering, 10.","DOI":"10.3390\/bioengineering10020218"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"De Chiara, F., Ferret-Mi\u00f1ana, A., Fern\u00e1ndez-Costa, J.M., and Ram\u00f3n-Azc\u00f3n, J. (2024). The Tissue Engineering Revolution: From Bench Research to Clinical Reality. Biomedicines, 12.","DOI":"10.3390\/biomedicines12020453"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Abolhasani, S., Ahmadi, Y., Rostami, Y., Baravar, E., and Fattahi, D. (2025). Biomaterials in tissue repair and regeneration: Key insights from extracellular matrix biology. Front. Med. Technol., 7.","DOI":"10.3389\/fmedt.2025.1565810"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"100003","DOI":"10.1016\/j.ccwv.2025.100003","article-title":"Exploring biomaterials for healthcare: An extensive insight into capabilities and applications","volume":"1","author":"Haleem","year":"2025","journal-title":"Cure Care"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"762","DOI":"10.1016\/j.progpolymsci.2007.05.017","article-title":"Biodegradable polymers as biomaterials","volume":"32","author":"Nair","year":"2007","journal-title":"Prog. Polym. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"5077","DOI":"10.1021\/acsabm.2c00793","article-title":"Sterilization of Polymeric Implants: Challenges and Opportunities","volume":"5","author":"Herczeg","year":"2022","journal-title":"ACS Appl. Bio Mater."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1010","DOI":"10.1007\/s11426-022-1243-5","article-title":"Biomedical polymers: Synthesis, properties, and applications","volume":"65","author":"Chen","year":"2022","journal-title":"Sci. China Chem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"521","DOI":"10.13005\/bbra\/2766","article-title":"Tissue Engineering: New Paradigm of Biomedicine","volume":"16","author":"Gautam","year":"2019","journal-title":"Biosci. Biotechnol. Res. Asia"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Kurowiak, J., Klekiel, T., and B\u0119dzi\u0144ski, R. (2023). Biodegradable Polymers in Biomedical Applications: A Review\u2014Developments, Perspectives and Future Challenges. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms242316952"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Abdulsalam, L., Abubakar, S., Permatasari, I., Lawal, A.A., Uddin, S., Ullah, S., and Ahmad, I. (2025). Advanced Biocompatible and Biodegradable Polymers: A Review of Functionalization, Smart Systems, and Sustainable Applications. Polymers, 17.","DOI":"10.3390\/polym17212901"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Ranakoti, L., Gangil, B., Bhandari, P., Singh, T., Sharma, S., Singh, J., and Singh, S. (2023). Promising Role of Polylactic Acid as an Ingenious Biomaterial in Scaffolds, Drug Delivery, Tissue Engineering, and Medical Implants: Research Developments, and Prospective Applications. Molecules, 28.","DOI":"10.3390\/molecules28020485"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Ranakoti, L., Gangil, B., Mishra, S.K., Singh, T., Sharma, S., Ilyas, R.A., and El-Khatib, S. (2022). Critical Review on Polylactic Acid: Properties, Structure, Processing, Biocomposites, and Nanocomposites. Materials, 15.","DOI":"10.3390\/ma15124312"},{"key":"ref_15","first-page":"471","article-title":"Literature Review: Ring Opening Polymerization of Polylactide","volume":"5","author":"Kaur","year":"2018","journal-title":"Int. J. Emerg. Technol. Innov. Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1002\/pen.20331","article-title":"Crystallinity and mechanical properties of optically pure polylactides and their blends","volume":"45","author":"Sarasua","year":"2005","journal-title":"Polym. Eng. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Capuana, E., Lopresti, F., Ceraulo, M., and La Carrubba, V. (2022). Poly-l-Lactic Acid (PLLA)-Based Biomaterials for Regenerative Medicine: A Review on Processing and Applications. Polymers, 14.","DOI":"10.3390\/polym14061153"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"3","DOI":"10.37190\/ABB-02448-2024-01","article-title":"Comparison of two polymers PDO and PLLA\/PCL in application of urological stent for the treatment of male urethral stenosis","volume":"26","author":"Kurowiak","year":"2024","journal-title":"Acta Bioeng. Biomech."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2352","DOI":"10.1007\/s10924-020-01749-0","article-title":"Improvement of Endothelial Cell Performance in an Optimized Electrospun Pre-polyglycerol Sebacate-Poly Lactic Acid Scaffold for Reconstruction of Intima in Coronary Arteries","volume":"28","author":"Gorgani","year":"2020","journal-title":"J. Polym. Environ."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"S923","DOI":"10.21037\/jtd.2017.06.30","article-title":"Mechanical behavior of polymer-based vs. metallic-based bioresorbable stents","volume":"9","author":"Ang","year":"2017","journal-title":"J. Thorac. Dis."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2818","DOI":"10.1021\/acs.macromol.3c02580","article-title":"Crystallinity-Independent Toughness in Renewable Poly(L-lactide) Triblock Plastics","volume":"57","author":"Krajovic","year":"2024","journal-title":"Macromolecules"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1007\/s40204-014-0026-7","article-title":"Bone tissue engineering scaffolding: Computer-aided scaffolding techniques","volume":"3","author":"Thavornyutikarn","year":"2014","journal-title":"Prog. Biomater."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Teixeira, M.A., Amorim, M.T.P., and Felgueiras, H.P. (2020). Poly(Vinyl Alcohol)-Based Nanofibrous Electrospun Scaffolds for Tissue Engineering Applications. Polymers, 12.","DOI":"10.3390\/polym12010007"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1016\/j.arabjc.2016.09.021","article-title":"Fabrication of highly porous biodegradable biomimetic nanocomposite as advanced bone tissue scaffold","volume":"10","author":"Khalil","year":"2017","journal-title":"Arab. J. Chem."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1186\/s13018-018-0724-2","article-title":"Scaffolds for the repair of bone defects in clinical studies: A systematic review","volume":"13","author":"Zeng","year":"2018","journal-title":"J. Orthop. Surg. Res."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1007\/s42242-020-00094-4","article-title":"The journey of multifunctional bone scaffolds fabricated from traditional toward modern techniques","volume":"3","author":"Bigham","year":"2020","journal-title":"Bio-Des. Manuf."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2439","DOI":"10.3390\/ijms13022439","article-title":"Enhancing Osteoconduction of PLLA-Based Nanocomposite Scaffolds for Bone Regeneration Using Different Biomimetic Signals to MSCs","volume":"13","author":"Ciapetti","year":"2012","journal-title":"Int. J. Mol. Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"935149","DOI":"10.1155\/2014\/935149","article-title":"A Comparative Study of Bioartificial Bone TissuePoly-L-lactic Acid\/Polycaprolactone and PLLA Scaffolds Applied in Bone Regeneration","volume":"2014","author":"Weng","year":"2014","journal-title":"J. Nanomater."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2073","DOI":"10.1166\/jbn.2013.1736","article-title":"Bone healing evaluation of nanofibrous composite scaffolds in rat calvarial defects: A comparative study","volume":"9","author":"Jaiswal","year":"2013","journal-title":"J. Biomed. Nanotechnol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1128","DOI":"10.1007\/s10439-021-02752-9","article-title":"Advances in the Fabrication of Scaffold and 3D Printing of Biomimetic Bone Graft","volume":"49","author":"Bisht","year":"2021","journal-title":"Ann. Biomed. Eng."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1007\/s13770-016-0002-5","article-title":"Solid Freeform Techniques Application in Bone Tissue Engineering for Scaffold Fabrication","volume":"14","author":"Shivalkar","year":"2017","journal-title":"Tissue Eng. Regen. Med."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"560","DOI":"10.1007\/s13238-017-0377-7","article-title":"The minor collagens in articular cartilage","volume":"8","author":"Luo","year":"2017","journal-title":"Protein Cell"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1007\/s00449-016-1724-4","article-title":"Strategies on process engineering of chondrocyte culture for cartilage tissue regeneration","volume":"40","author":"Mallick","year":"2017","journal-title":"Bioprocess Biosyst. Eng."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1007\/s10616-015-9895-4","article-title":"Scaffolds and cells for tissue regeneration: Different scaffold pore sizes\u2014Different cell effects","volume":"68","author":"Bagdonas","year":"2016","journal-title":"Cytotechnology"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/j.matdes.2018.06.012","article-title":"Layered gelatin\/PLLA scaffolds fabricated by electrospinning and 3D printing- for nasal cartilages and subchondral bone reconstruction","volume":"155","author":"Rajzer","year":"2018","journal-title":"Mater. Des."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1080\/15685551.2015.1136535","article-title":"Evaluation of poly(L-lactide) and chitosan composite scaffolds for cartilage tissue regeneration","volume":"19","author":"Mallick","year":"2016","journal-title":"Des. Monomers Polym."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1016\/j.msec.2017.06.011","article-title":"PLLA scaffolds produced by thermally induced phase separation (TIPS) allow human chondrocyte growth and extracellular matrix formation dependent on pore size","volume":"80","author":"Conoscenti","year":"2017","journal-title":"Mater. Sci. Eng. C."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1186\/scrt156","article-title":"Vascular tissue engineering: Biodegradable scaffold platforms to promote angiogenesis","volume":"4","author":"Serbo","year":"2013","journal-title":"Stem Cell Res. Ther."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1177\/0885328216652068","article-title":"In vitro hemocompatibility and cytocompatibility of a three-layered vascular scaffold fabricated by sequential electrospinning of PCL, collagen, and PLLA nanofibers","volume":"31","author":"Javanmard","year":"2016","journal-title":"J. Biomater. Appl."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Vedeanu, N.S., Lujerdean, C., Z\u0103han, M., Dezmirean, D.S., Barbu-Tudoran, L., Damian, G., and \u0218tefan, R. (2022). Synthesis and Structural Characterization of CaO-P2O5-CaF:CuO Glasses with Antitumoral Effect on Skin Cancer Cells. Materials, 15.","DOI":"10.3390\/ma15041526"},{"key":"ref_41","unstructured":"(2025). Plastics\u2014Determination of Tensile Properties (Standard No. ISO 527)."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"6567","DOI":"10.1007\/s10853-013-7453-z","article-title":"Biodegradable electrospun PLLA\/chitosan membrane as guided tissue regeneration membrane for treating periodontitis","volume":"48","author":"Chen","year":"2013","journal-title":"J. Mater. Sci."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"104409","DOI":"10.1016\/j.jmbbm.2021.104409","article-title":"Physical and mechanical degradation behaviour of semi-crystalline PLLA for bioresorbable stent applications","volume":"118","author":"Abaei","year":"2021","journal-title":"J. Mech. Behav. Biomed. Mater."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Malone, L.P., Best, S.M., and Cameron, R.E. (2024). Accelerated degradation testing impacts the degradation processes in 3D printed amorphous PLLA. Front. Bioeng. Biotechnol., 12.","DOI":"10.3389\/fbioe.2024.1419654"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"5483","DOI":"10.1007\/s00170-025-16465-z","article-title":"Elaboration of PLA and PLLA stents manufacturing regimes and evaluation of their mechanical performance","volume":"140","author":"Kuchumov","year":"2025","journal-title":"Int. J. Adv. Manuf. Technol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"100615","DOI":"10.1016\/j.nxmate.2025.100615","article-title":"Hydrolytic degradation studies of bio-composites of poly(L-lactic acid)\/Oligo(L-lactic acid) grafted and non-grafted microcrystalline cellulose","volume":"8","author":"Rahaman","year":"2025","journal-title":"Next Mater."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1016\/j.addr.2016.06.012","article-title":"Physical and mechanical properties of PLA, and their functions in widespread applications\u2014A comprehensive review","volume":"107","author":"Farah","year":"2016","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1364","DOI":"10.1016\/j.polymdegradstab.2009.05.015","article-title":"Effect of microstructure on hydrolytic degradation studies of poly(L-lactic acid) by FTIR spectroscopy and differential scanning calorimetry","volume":"94","author":"Vasanthan","year":"2009","journal-title":"Polym. Degrad. Stab."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1002\/jbm.b.31419","article-title":"Spectroscopy Techniques for Analyzing the Hydrolysis of PLGA and PLLA","volume":"91B","author":"Tan","year":"2009","journal-title":"J. Biomed. Mater. Res. B Appl. Biomater."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"5590","DOI":"10.1039\/C4AY01289E","article-title":"Attenuated total reflectance\/Fourier transform infrared (ATR\/FTIR) mapping coupled with principal component analysis for the study of in vitro degradation of porous polylactide\/hydroxyapatite composite material","volume":"6","author":"Jing","year":"2014","journal-title":"Anal. 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