{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T21:54:06Z","timestamp":1791323646286,"version":"4.3.1"},"reference-count":15,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2011,9,7]],"date-time":"2011-09-07T00:00:00Z","timestamp":1315353600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Materials"],"abstract":"<jats:p>Inulin is a polysaccharide contained in chicory, dahlia, and other plants. An oligosaccharide DFA III is produced from inulin using a microbial enzyme, inulin fructotransferase (DFA III producing) [EC 2.4.1.93]. The oligosaccharide DFAIII has a unique functionality that accelerates the assimilation of minerals (Ca, Fe, and so on) from intestines. Therefore, it has a potential for the improvement of osteoporosis and iron deficiency anemia. The production of DFA III was industrialized in 2004 in Japan. Another oligosaccharide DFA I is produced from inulin by another enzyme, inulin fructotransferase (DFA I producing) [EC 2.4.1.200]. The oligosaccharide DFA I has half the sweetness of sucrose. The genes of the two enzymes were cloned and the nucleotide sequences were determined. The deduced amino acid sequences of two enzyme genes had a homology of 49.8%.<\/jats:p>","DOI":"10.3390\/ma4091543","type":"journal-article","created":{"date-parts":[[2011,9,7]],"date-time":"2011-09-07T07:59:55Z","timestamp":1315382395000},"page":"1543-1547","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Two Types of Inulin Fructotransferases"],"prefix":"10.3390","volume":"4","author":[{"given":"Kazutomo","family":"Haraguchi","sequence":"first","affiliation":[{"name":"National Food Research Institute, 2-1-12 Kannondai, Tsukuba-shi, Ibaraki 305-8642, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2011,9,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1016\/0005-2744(73)90271-4","article-title":"Purification and properties of Arthrobacter ureafaciens inulase II","volume":"315","author":"Uchiyama","year":"1973","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_2","first-page":"291","article-title":"Purification and properties of inulin fructotransferase (depolymerizing) from Arthrobacter globiformis C11-1","volume":"52","author":"Haraguchi","year":"1988","journal-title":"Agric. Biol. Chem."},{"key":"ref_3","first-page":"3209","article-title":"Purification and some properties of inulin fructotransferase (depolymerizing) from Arthrobacter ilicis","volume":"52","author":"Kawamura","year":"1988","journal-title":"Agric. Biol. Chem."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1016\/0922-338X(91)90160-I","article-title":"Purification and properties of an inulin fructotransferase (depolymerizing) from Arthrobacter sp. H65-7","volume":"72","author":"Yokota","year":"1991","journal-title":"J. Ferment. Bioeng."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1016\/j.carbpol.2004.06.046","article-title":"Thermostable inulin fructotransferase (DFA III-producing) from Arthrobacter sp. L68-1","volume":"59","author":"Haraguchi","year":"2005","journal-title":"Carbohydr. Polym."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"628","DOI":"10.1271\/bbb.62.628","article-title":"Purification and properties of inulin fructotransferase (DFA III-producing) from Bacillus sp. snu-7","volume":"62","author":"Kang","year":"1998","journal-title":"Biosci. Biotech. Biochem."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.carbpol.2006.02.027","article-title":"Inulin fructotransferase (DFA III-producing) from Leifsonia sp. T88-4","volume":"66","author":"Haraguchi","year":"2006","journal-title":"Carbohydr. Polym."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1271\/bbb.61.87","article-title":"Molecular cloning of an inulin fructotransfarase (depolymerizing) gene from Arthrobacter sp. H65-7 and its expression in Escherichia coli","volume":"61","author":"Sakurai","year":"1997","journal-title":"Biosci. Biotech. Biochem."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1016\/S1389-1723(00)80062-6","article-title":"Cloning of inulin fructotransferase (DFA III-producing) gene from Arthrobacter globiformis C11-1","volume":"89","author":"Haraguchi","year":"2000","journal-title":"J. Biosci. Bioeng."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1321","DOI":"10.1271\/bbb.64.1321","article-title":"Difructose anhydrides: Their mass production and physiological functions","volume":"64","author":"Saito","year":"2000","journal-title":"Biosci. Biotech. Biochem."},{"key":"ref_11","first-page":"2089","article-title":"Purification and properties of a novel inulin fructotransferase (DFA I-producing) from Arthrobacter globiformis S14-3","volume":"53","author":"Seki","year":"1989","journal-title":"Agric. Biol. Chem."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"574","DOI":"10.1271\/bbb.58.574","article-title":"Purification of inulin fructotransferase (DFA I-producing) from Arthrobacter sp. MCI-2493 and production of DFA I from inulin by the enzyme","volume":"58","author":"Ueda","year":"1994","journal-title":"Biosci. Biotech. Biochem."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1049","DOI":"10.1023\/A:1024138428965","article-title":"Purification and properties of a heat-stable inulin fructotransferase from Arthrobacter ureafaciens","volume":"25","author":"Haraguchi","year":"2003","journal-title":"Biotech. 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Biochem."}],"container-title":["Materials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1996-1944\/4\/9\/1543\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T21:57:19Z","timestamp":1760219839000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1996-1944\/4\/9\/1543"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,9,7]]},"references-count":15,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2011,9]]}},"alternative-id":["ma4091543"],"URL":"https:\/\/doi.org\/10.3390\/ma4091543","relation":{},"ISSN":["1996-1944"],"issn-type":[{"value":"1996-1944","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,9,7]]}}}