{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T03:54:09Z","timestamp":1790222049020,"version":"4.1.0"},"reference-count":39,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2015,1,26]],"date-time":"2015-01-26T00:00:00Z","timestamp":1422230400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"FAPESP","doi-asserted-by":"publisher","award":["2011\/21442-6"],"award-info":[{"award-number":["2011\/21442-6"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Materials"],"abstract":"<jats:p>Synthetic and natural polymer association is a promising tool in tissue engineering. The aim of this study was to compare five methodologies for producing  hybrid scaffolds for cell culture using poly-l-lactide (PLLA) and collagen: functionalization of PLLA electrospun by (1) dialkylamine and collagen immobilization with glutaraldehyde and by (2) hydrolysis and collagen immobilization with carbodiimide chemistry;  (3) co-electrospinning of PLLA\/chloroform and collagen\/hexafluoropropanol (HFP) solutions; (4) co-electrospinning of PLLA\/chloroform and collagen\/acetic acid solutions and (5) electrospinning of a co-solution of PLLA and collagen using HFP. These materials were evaluated based on their morphology, mechanical properties, ability to induce cell proliferation and alkaline phosphatase activity upon submission of mesenchymal stem cells to basal or osteoblastic differentiation medium (ODM). Methods (1) and (2) resulted in a decrease in mechanical properties, whereas methods (3), (4) and (5) resulted in materials of higher tensile strength and osteogenic differentiation. Materials yielded by methods (2),  (3) and (5) promoted osteoinduction even in the absence of ODM. The results indicate that the scaffold based on the PLLA\/collagen blend exhibited optimal mechanical properties  and the highest capacity for osteodifferentiation and was the best choice for collagen incorporation into PLLA in bone repair applications.<\/jats:p>","DOI":"10.3390\/ma8020408","type":"journal-article","created":{"date-parts":[[2015,1,26]],"date-time":"2015-01-26T13:26:37Z","timestamp":1422278797000},"page":"408-423","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Hybrid Membranes of PLLA\/Collagen for Bone Tissue Engineering: A Comparative Study of Scaffold Production Techniques for Optimal Mechanical Properties and Osteoinduction Ability"],"prefix":"10.3390","volume":"8","author":[{"given":"Fl\u00e1via","family":"Gon\u00e7alves","sequence":"first","affiliation":[{"name":"Instituto de Qu\u00edmica, Universidade de S\u00e3o Paulo, Av. Prof. Lineu Prestes, 748,  05508-000 S\u00e3o Paulo, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo","family":"Bentini","sequence":"additional","affiliation":[{"name":"Instituto de Qu\u00edmica, Universidade de S\u00e3o Paulo, Av. Prof. Lineu Prestes, 748,  05508-000 S\u00e3o Paulo, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana","family":"Burrows","sequence":"additional","affiliation":[{"name":"Instituto de Qu\u00edmica, Universidade de S\u00e3o Paulo, Av. Prof. Lineu Prestes, 748,  05508-000 S\u00e3o Paulo, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Carreira","sequence":"additional","affiliation":[{"name":"Instituto de Qu\u00edmica, Universidade de S\u00e3o Paulo, Av. Prof. Lineu Prestes, 748,  05508-000 S\u00e3o Paulo, SP, Brazil"},{"name":"Faculdade de Medicina, N\u00facleo de Terapia Celular e Molecular (NUCEL)\u2014N\u00facleo de Estudos e Terapia Celular e Molecular (NETCEM), Universidade de S\u00e3o Paulo, Rua Pangar\u00e9 100,  05360-130 S\u00e3o Paulo, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"Kossugue","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina, N\u00facleo de Terapia Celular e Molecular (NUCEL)\u2014N\u00facleo de Estudos e Terapia Celular e Molecular (NETCEM), Universidade de S\u00e3o Paulo, Rua Pangar\u00e9 100,  05360-130 S\u00e3o Paulo, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mari","family":"Sogayar","sequence":"additional","affiliation":[{"name":"Instituto de Qu\u00edmica, Universidade de S\u00e3o Paulo, Av. Prof. Lineu Prestes, 748,  05508-000 S\u00e3o Paulo, SP, Brazil"},{"name":"Faculdade de Medicina, N\u00facleo de Terapia Celular e Molecular (NUCEL)\u2014N\u00facleo de Estudos e Terapia Celular e Molecular (NETCEM), Universidade de S\u00e3o Paulo, Rua Pangar\u00e9 100,  05360-130 S\u00e3o Paulo, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiz","family":"Catalani","sequence":"additional","affiliation":[{"name":"Instituto de Qu\u00edmica, Universidade de S\u00e3o Paulo, Av. Prof. Lineu Prestes, 748,  05508-000 S\u00e3o Paulo, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2015,1,26]]},"reference":[{"key":"ref_1","unstructured":"Formhals, A. (1934). Process and Apparatus for Preparing Artificial Threads. 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A"}],"container-title":["Materials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1996-1944\/8\/2\/408\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T20:41:59Z","timestamp":1760215319000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1996-1944\/8\/2\/408"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,1,26]]},"references-count":39,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2015,2]]}},"alternative-id":["ma8020408"],"URL":"https:\/\/doi.org\/10.3390\/ma8020408","relation":{},"ISSN":["1996-1944"],"issn-type":[{"value":"1996-1944","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,1,26]]}}}