{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T14:30:51Z","timestamp":1790173851375,"version":"4.1.0"},"reference-count":46,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2013,4,23]],"date-time":"2013-04-23T00:00:00Z","timestamp":1366675200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>Protein tyrosine phosphatase 1B (PTP1B) plays a major role in the negative regulation of insulin signaling, and is thus considered as an attractive therapeutic target for the treatment of diabetes. Bioassay-guided investigation of the methylethylketone extract of marine-derived fungus Penicillium sp. JF-55 cultures afforded a new PTP1B inhibitory styrylpyrone-type metabolite named penstyrylpyrone (1), and two known metabolites, anhydrofulvic acid (2) and citromycetin (3). Compounds 1 and 2 inhibited PTP1B activity in a dose-dependent manner, and kinetic analyses of PTP1B inhibition suggested that these compounds inhibited PTP1B activity in a competitive manner. In an effort to gain more biological potential of the isolated compounds, the anti-inflammatory effects of compounds 1\u20133 were also evaluated. Among the tested compounds, only compound 1 inhibited the production of NO and PGE2, due to the inhibition of the expression of iNOS and  COX-2. Penstyrylpyrone (1) also reduced TNF-\u03b1 and IL-1\u03b2 production, and these  anti-inflammatory effects were shown to be correlated with the suppression of the phosphorylation and degradation of I\u03baB-\u03b1, NF-\u03baB nuclear translocation, and NF-\u03baB DNA binding activity. In addition, using inhibitor tin protoporphyrin (SnPP), an inhibitor of HO-1, it was verified that the inhibitory effects of penstyrylpyrone (1) on the pro-inflammatory mediators and NF-\u03baB DNA binding activity were associated with the HO-1 expression. Therefore, these results suggest that penstyrylpyrone (1) suppresses PTP1B activity, as well as the production of pro-inflammatory mediators via NF-\u03baB pathway, through expression of anti-inflammatory HO-1.<\/jats:p>","DOI":"10.3390\/md11041409","type":"journal-article","created":{"date-parts":[[2013,4,23]],"date-time":"2013-04-23T06:22:31Z","timestamp":1366698151000},"page":"1409-1426","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":59,"title":["PTP1B Inhibitory and Anti-Inflammatory Effects of  Secondary Metabolites Isolated from the Marine-Derived Fungus Penicillium sp. JF-55"],"prefix":"10.3390","volume":"11","author":[{"given":"Dong-Sung","family":"Lee","sequence":"first","affiliation":[{"name":"Hanbang Body-Fluid Research Center, Wonkwang University, Iksan 570-749, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jae-Hyuk","family":"Jang","sequence":"additional","affiliation":[{"name":"Chemical Biology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 30 Yeongudanji-ro, Ochang, Cheongwon 363-883, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wonmin","family":"Ko","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Wonkwang University, Iksan 570-749, Korea"},{"name":"Standardized Material Bank for New Botanical Drugs, College of Pharmacy,  Wonkwang University, Iksan 570-749, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyoung-Su","family":"Kim","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Wonkwang University, Iksan 570-749, Korea"},{"name":"Standardized Material Bank for New Botanical Drugs, College of Pharmacy,  Wonkwang University, Iksan 570-749, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jae","family":"Sohn","sequence":"additional","affiliation":[{"name":"College of Medical and Life Sciences, Silla University, Busan 617-736, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Myeong-Suk","family":"Kang","sequence":"additional","affiliation":[{"name":"College of Medical and Life Sciences, Silla University, Busan 617-736, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jong","family":"Ahn","sequence":"additional","affiliation":[{"name":"Chemical Biology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 30 Yeongudanji-ro, Ochang, Cheongwon 363-883, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youn-Chul","family":"Kim","sequence":"additional","affiliation":[{"name":"Hanbang Body-Fluid Research Center, Wonkwang University, Iksan 570-749, Korea"},{"name":"College of Pharmacy, Wonkwang University, Iksan 570-749, Korea"},{"name":"Standardized Material Bank for New Botanical Drugs, College of Pharmacy,  Wonkwang University, Iksan 570-749, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyuncheol","family":"Oh","sequence":"additional","affiliation":[{"name":"Hanbang Body-Fluid Research Center, Wonkwang University, Iksan 570-749, Korea"},{"name":"College of Pharmacy, Wonkwang University, Iksan 570-749, Korea"},{"name":"Standardized Material Bank for New Botanical Drugs, College of Pharmacy,  Wonkwang University, Iksan 570-749, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2013,4,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"210","DOI":"10.3390\/md7020210","article-title":"Antitumor compounds from marine actinomycetes","volume":"7","author":"Olano","year":"2009","journal-title":"Mar. 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