{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T07:29:07Z","timestamp":1775633347991,"version":"3.50.1"},"reference-count":27,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2013,10,22]],"date-time":"2013-10-22T00:00:00Z","timestamp":1382400000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>The benzopyran compound obtained by cultivating a mangrove-derived strain, Streptomyces xiamenensis strain 318, shows multiple biological effects, including  anti-fibrotic and anti-hypertrophic scar properties. To increase the diversity in the structures of the available benzopyrans, by means of biosynthesis, the strain was screened for spontaneous rifampicin resistance (Rif), and a mutated rpsL gene to confer streptomycin resistance (Str), was introduced into the S. xiamenensis strain M1-94P  that originated from deep-sea sediments. Two new benzopyran derivatives, named xiamenmycin C (1) and D (2), were isolated from the crude extracts of a selected Str-Rif double mutant (M6) of M1-94P. The structures of 1 and 2 were identified by analyzing extensive spectroscopic data. Compounds 1 and 2 both inhibit the proliferation of human lung fibroblasts (WI26), and 1 exhibits better anti-fibrotic activity than xiamenmycin. Our study presents the novel bioactive compounds isolated from S. xiamenensis mutant strain M6 constructed by ribosome engineering, which could be a useful approach in the discovery of new anti-fibrotic compounds.<\/jats:p>","DOI":"10.3390\/md11104035","type":"journal-article","created":{"date-parts":[[2013,10,23]],"date-time":"2013-10-23T07:45:42Z","timestamp":1382514342000},"page":"4035-4049","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Identification of Two Novel Anti-Fibrotic Benzopyran Compounds Produced by Engineered Strains Derived from Streptomyces xiamenensis M1-94P that Originated from  Deep-Sea Sediments"],"prefix":"10.3390","volume":"11","author":[{"given":"Zhong-Yuan","family":"You","sequence":"first","affiliation":[{"name":"State Key Laboratory of Microbial Metabolism and School of Life Science & Biotechnology,  State Key Laboratory of Ocean Engineering, Shanghai Jiao Tong University, Shanghai 200240, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ya-Hui","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200240, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhi-Gang","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200240, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min-Juan","family":"Xu","sequence":"additional","affiliation":[{"name":"Ministry of Education Key Laboratory of Systems Biomedicine, Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, Shanghai 200240, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shu-Jie","family":"Xie","sequence":"additional","affiliation":[{"name":"Key Laboratory of Marine Biogenetic Resources, the Third Institute of Oceanography SOA, Xiamen 361005, Fujian, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tie-Sheng","family":"Han","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Metabolism and School of Life Science & Biotechnology,  State Key Laboratory of Ocean Engineering, Shanghai Jiao Tong University, Shanghai 200240, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Feng","sequence":"additional","affiliation":[{"name":"Instrumental Analysis Center, Shanghai Jiao Tong University, Shanghai 200240, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xue-Gong","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Metabolism and School of Life Science & Biotechnology,  State Key Laboratory of Ocean Engineering, Shanghai Jiao Tong University, Shanghai 200240, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Xu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Metabolism and School of Life Science & Biotechnology,  State Key Laboratory of Ocean Engineering, Shanghai Jiao Tong University, Shanghai 200240, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2013,10,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1055\/s-2006-957384","article-title":"Plant-derived leading compounds for chemotherapy of human immunodeficiency virus (HIV) infection","volume":"64","author":"Vlietinck","year":"1998","journal-title":"Planta Med."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Greim, H., and Snyder, R. 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