{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T10:29:57Z","timestamp":1790418597193,"version":"4.1.0"},"reference-count":45,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2014,3,24]],"date-time":"2014-03-24T00:00:00Z","timestamp":1395619200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>Surface-associated marine bacteria are an interesting source of new secondary metabolites. The aim of this study was the isolation and identification of epiphytic bacteria from the marine brown alga, Bifurcaria bifurcata, and the evaluation of the antioxidant and antimicrobial activity of bacteria extracts. The identification of epiphytic bacteria was determined by 16S rRNA gene sequencing. Bacteria extracts were obtained with methanol and dichloromethane (1:1) extraction. The antioxidant activity of extracts was performed by quantification of total phenolic content (TPC), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and oxygen radical absorbance capacity (ORAC). Antimicrobial activities were evaluated against Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, Salmonella enteritidis, Staphylococcus aureus, Saccharomyces cerevisiae and Candida albicans. A total of 39 Bifurcaria bifurcata-associated bacteria were isolated and 33 were identified as Vibrio sp. (48.72%), Alteromonas sp. (12.82%), Shewanella sp. (12.26%), Serratia sp. (2.56%), Citricoccus sp. (2.56%), Cellulophaga sp. (2.56%), Ruegeria sp. (2.56%) and Staphylococcus sp. (2.56%). Six (15.38%) of the 39 bacteria Bifurcaria bifurcata-associated bacteria presented less than a 90% Basic Local Alignment Search Tool (BLAST) match, and some of those could be new. The highest antioxidant activity and antimicrobial activity (against B. subtilis) was exhibited by strain 16 (Shewanella sp.). Several strains also presented high antimicrobial activity against  S. aureus, mainly belonging to Alteromonas sp. and Vibrio sp. There were no positive results against fungi and Gram-negative bacteria. Bifurcaria bifurcata epiphytic bacteria were revealed to be excellent sources of natural antioxidant and antimicrobial compounds.<\/jats:p>","DOI":"10.3390\/md12031676","type":"journal-article","created":{"date-parts":[[2014,3,24]],"date-time":"2014-03-24T13:43:41Z","timestamp":1395668621000},"page":"1676-1689","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":61,"title":["Antioxidant and Antimicrobial Potential of the  Bifurcaria bifurcata Epiphytic Bacteria"],"prefix":"10.3390","volume":"12","author":[{"given":"Andr\u00e9","family":"Horta","sequence":"first","affiliation":[{"name":"Marine Resources Research Group (GIRM), School of Tourism and Maritime Technology (ESTM), Polytechnic Institute of Leiria, 2520-641 Peniche, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3290-3111","authenticated-orcid":false,"given":"Susete","family":"Pinteus","sequence":"additional","affiliation":[{"name":"Marine Resources Research Group (GIRM), School of Tourism and Maritime Technology (ESTM), Polytechnic Institute of Leiria, 2520-641 Peniche, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Celso","family":"Alves","sequence":"additional","affiliation":[{"name":"Marine Resources Research Group (GIRM), School of Tourism and Maritime Technology (ESTM), Polytechnic Institute of Leiria, 2520-641 Peniche, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N\u00e1dia","family":"Fino","sequence":"additional","affiliation":[{"name":"Marine Resources Research Group (GIRM), School of Tourism and Maritime Technology (ESTM), Polytechnic Institute of Leiria, 2520-641 Peniche, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joana","family":"Silva","sequence":"additional","affiliation":[{"name":"Marine Resources Research Group (GIRM), School of Tourism and Maritime Technology (ESTM), Polytechnic Institute of Leiria, 2520-641 Peniche, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Fernandez","sequence":"additional","affiliation":[{"name":"Higher School of Agricultural Engineering (ETSEA), University of Lleida, E-25003 Lleida, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Am\u00e9rico","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Marine Resources Research Group (GIRM), School of Tourism and Maritime Technology (ESTM), Polytechnic Institute of Leiria, 2520-641 Peniche, Portugal"},{"name":"Gulbenkian Institute of Science, 2780-156 Oeira, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Pedrosa","sequence":"additional","affiliation":[{"name":"Marine Resources Research Group (GIRM), School of Tourism and Maritime Technology (ESTM), Polytechnic Institute of Leiria, 2520-641 Peniche, Portugal"},{"name":"Center of Pharmacology and Chemical Biopathology, Faculty of Medicine, University of Porto, 4200-319 Porto, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2014,3,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"544","DOI":"10.1111\/j.1751-7915.2010.00179.x","article-title":"Bioactive compounds from marine bacteria and fungi","volume":"3","author":"Debbab","year":"2010","journal-title":"Microb. 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