{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T18:39:55Z","timestamp":1790793595303,"version":"4.1.0"},"reference-count":41,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2014,12,29]],"date-time":"2014-12-29T00:00:00Z","timestamp":1419811200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>A phytochemical investigation of a southern Australian marine brown alga, Sargassum paradoxum, resulted in the isolation and identification of four new (5, 9, 10, and 15) and nine previously reported (1, 2, 6\u20138, and 11\u201314) bioactive meroditerpenoids.  HPLC-NMR and HPLC-MS were central to the identification of a new unstable compound, sargahydroquinal (9), and pivotal in the deconvolution of eight (1, 2, 5\u20137, and 10\u201312) other meroditerpenoids. In particular, the complete characterization and identification of the two main constituents (1 and 2) in the crude dichloromethane extract was achieved using stop-flow HPLC-NMR and HPLC-MS. This study resulted in the first acquisition of gHMBCAD NMR spectra in the stop-flow HPLC-NMR mode for a system solely equipped with a 60 \u03bcL HPLC-NMR flow cell without the use of a cold probe, microcoil, or any pre-concentration.<\/jats:p>","DOI":"10.3390\/md13010102","type":"journal-article","created":{"date-parts":[[2014,12,29]],"date-time":"2014-12-29T10:58:34Z","timestamp":1419850714000},"page":"102-127","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Chemical Profiling (HPLC-NMR &amp; HPLC-MS), Isolation, and Identification of Bioactive Meroditerpenoids from the Southern Australian Marine Brown Alga Sargassum paradoxum"],"prefix":"10.3390","volume":"13","author":[{"given":"Robert","family":"Brklja\u010da","sequence":"first","affiliation":[{"name":"School of Applied Sciences (Discipline of Chemistry), Health Innovations Research Institute (HIRi) RMIT University, GPO Box 2476V Melbourne, Victoria 3001, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvia","family":"Urban","sequence":"additional","affiliation":[{"name":"School of Applied Sciences (Discipline of Chemistry), Health Innovations Research Institute (HIRi) RMIT University, GPO Box 2476V Melbourne, Victoria 3001, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2014,12,29]]},"reference":[{"key":"ref_1","unstructured":"(2014). 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A"}],"container-title":["Marine Drugs"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1660-3397\/13\/1\/102\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T21:11:49Z","timestamp":1760217109000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1660-3397\/13\/1\/102"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,12,29]]},"references-count":41,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2015,1]]}},"alternative-id":["md13010102"],"URL":"https:\/\/doi.org\/10.3390\/md13010102","relation":{},"ISSN":["1660-3397"],"issn-type":[{"value":"1660-3397","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,12,29]]}}}