{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T13:23:12Z","timestamp":1781529792147,"version":"3.54.1"},"reference-count":42,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2015,12,30]],"date-time":"2015-12-30T00:00:00Z","timestamp":1451433600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005694","name":"Hori Sciences and Arts Foundation","doi-asserted-by":"publisher","award":["260312"],"award-info":[{"award-number":["260312"]}],"id":[{"id":"10.13039\/501100005694","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>The blood coagulation cascade involves the human coagulation factors thrombin and an activated factor VII (fVIIa). Thrombin and fVIIa are vitamin-K-dependent clotting factors associated with bleeding, bleeding complications and disorders. Thrombin and fVIIa cause excessive bleeding when treated with vitamin-K antagonists. In this research, we explored different strains of toxic Microcystis aeruginosa and cyanobacteria blooms for the probable fVIIa-soluble Tissue Factor (fVIIa-sTF) inhibitors. The algal cells were subjected to acidification, and reverse phase (ODS) chromatography-solid phase extraction eluted by water to 100% MeOH with 20%-MeOH increments except for M. aeruginosa NIES-89, from the National Institute for Environmental Studies (NIES), which was eluted with 5%-MeOH increments as an isolation procedure to separate aeruginosins 89A and B from co-eluting microcystins. The 40%\u201380% MeOH fractions of the cyanobacterial extract are active against fVIIa-sTF. The fVIIa-sTF active fractions from cultured cyanobacteria and cyanobacteria blooms were subjected to liquid chromatography-mass spectrometry (LC-MS). The 60% MeOH fraction of M. aeruginosa K139 exhibited an m\/z 603 [M + H]+ attributed to aeruginosin K139, and the 40% MeOH fraction of M. aeruginosa NIES-89 displayed ions with m\/z 617 [M \u2212 SO3 + H]+ and m\/z [M + H]+ 717, which attributed to aeruginosin 89. Aeruginosins 102A\/B and 298A\/B were also observed from other toxic strains of M. aeruginosa with positive fVIIa-sTF inhibitory activity. The active fractions contained cyanobacterial peptides of the aeruginosin class as fVIIa-sTF inhibitors detected by LC-MS.<\/jats:p>","DOI":"10.3390\/md14010007","type":"journal-article","created":{"date-parts":[[2015,12,30]],"date-time":"2015-12-30T10:25:05Z","timestamp":1451471105000},"page":"7","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Determination of FVIIa-sTF Inhibitors in Toxic Microcystis Cyanobacteria by LC-MS Technique"],"prefix":"10.3390","volume":"14","author":[{"given":"Andrea","family":"Anas","sequence":"first","affiliation":[{"name":"Faculty of Pharmacy, Meijo University, Tempaku, Nagoya 468-8503, Japan"},{"name":"Department of Brain Functions, Division of Stress Adaptation and Protection, Research Institute of Environmental Medicine, Nagoya University, Nagoya 464-8601, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Nakajima","sequence":"additional","affiliation":[{"name":"Graduate School of Environmental and Human Sciences, Meijo University, Tempaku, Nagoya 468-8503, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiaki","family":"Naruse","sequence":"additional","affiliation":[{"name":"Graduate School of Environmental and Human Sciences, Meijo University, Tempaku, Nagoya 468-8503, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mineka","family":"Tone","sequence":"additional","affiliation":[{"name":"Graduate School of Environmental and Human Sciences, Meijo University, Tempaku, Nagoya 468-8503, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hirohiko","family":"Asukabe","sequence":"additional","affiliation":[{"name":"Graduate School of Environmental and Human Sciences, Meijo University, Tempaku, Nagoya 468-8503, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ken-ichi","family":"Harada","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Meijo University, Tempaku, Nagoya 468-8503, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2015,12,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"50819","DOI":"10.1074\/jbc.X300009200","article-title":"A brief historical review of the waterfall\/cascade of blood coagulation","volume":"278","author":"Davie","year":"2003","journal-title":"J. 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