{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T08:41:28Z","timestamp":1781685688706,"version":"3.54.5"},"reference-count":62,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2016,3,10]],"date-time":"2016-03-10T00:00:00Z","timestamp":1457568000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Union's Seventh Framework Programme (FP7\/2007-2013)","award":["311975"],"award-info":[{"award-number":["311975"]}]},{"DOI":"10.13039\/501100011697","name":"Brittany Region","doi-asserted-by":"publisher","award":["8433"],"award-info":[{"award-number":["8433"]}],"id":[{"id":"10.13039\/501100011697","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>The evolving global threat of antimicrobial resistance requires a deep renewal of the antibiotic arsenal including the isolation and characterization of new drugs. Underexplored marine ecosystems may represent an untapped reservoir of novel bioactive molecules. Deep-sea fungi isolated from a record-depth sediment core of almost 2000 m below the seafloor were investigated for antimicrobial activities. This antimicrobial screening, using 16 microbial targets, revealed 33% of filamentous fungi synthesizing bioactive compounds with activities against pathogenic bacteria and fungi. Interestingly, occurrence of antimicrobial producing isolates was well correlated with the complexity of the habitat (in term of microbial richness), as higher antimicrobial activities were obtained at specific layers of the sediment core. It clearly highlights complex deep-sea habitats as chemical battlefields where synthesis of numerous bioactive compounds appears critical for microbial competition. The six most promising deep subseafloor fungal isolates were selected for the production and extraction of bioactive compounds. Depending on the fungal isolates, antimicrobial compounds were only biosynthesized in semi-liquid or solid-state conditions as no antimicrobial activities were ever detected using liquid fermentation. An exception was made for one fungal isolate, and the extraction procedure designed to extract amphipathic compounds was successful and highlighted the amphiphilic profile of the bioactive metabolites.<\/jats:p>","DOI":"10.3390\/md14030050","type":"journal-article","created":{"date-parts":[[2016,3,10]],"date-time":"2016-03-10T10:53:48Z","timestamp":1457607228000},"page":"50","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Deep Subseafloor Fungi as an Untapped Reservoir of Amphipathic Antimicrobial Compounds"],"prefix":"10.3390","volume":"14","author":[{"given":"Marion","family":"Navarri","sequence":"first","affiliation":[{"name":"Laboratoire de Biodiversit\u00e9 et d\u2019Ecologie Microbienne (EA 3882), Universit\u00e9 de Brest, Technopole Brest Iroise, 29280 Plouzan\u00e9, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9964-9309","authenticated-orcid":false,"given":"Camille","family":"J\u00e9gou","sequence":"additional","affiliation":[{"name":"Laboratoire de Biodiversit\u00e9 et d\u2019Ecologie Microbienne (EA 3882), Universit\u00e9 de Brest, Technopole Brest Iroise, 29280 Plouzan\u00e9, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurence","family":"Meslet-Cladi\u00e8re","sequence":"additional","affiliation":[{"name":"Laboratoire de Biodiversit\u00e9 et d\u2019Ecologie Microbienne (EA 3882), Universit\u00e9 de Brest, Technopole Brest Iroise, 29280 Plouzan\u00e9, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Brillet","sequence":"additional","affiliation":[{"name":"Laboratoire de Biodiversit\u00e9 et d\u2019Ecologie Microbienne (EA 3882), Universit\u00e9 de Brest, Technopole Brest Iroise, 29280 Plouzan\u00e9, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georges","family":"Barbier","sequence":"additional","affiliation":[{"name":"Laboratoire de Biodiversit\u00e9 et d\u2019Ecologie Microbienne (EA 3882), Universit\u00e9 de Brest, Technopole Brest Iroise, 29280 Plouzan\u00e9, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ga\u00ebtan","family":"Burgaud","sequence":"additional","affiliation":[{"name":"Laboratoire de Biodiversit\u00e9 et d\u2019Ecologie Microbienne (EA 3882), Universit\u00e9 de Brest, Technopole Brest Iroise, 29280 Plouzan\u00e9, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1638-0963","authenticated-orcid":false,"given":"Yannick","family":"Fleury","sequence":"additional","affiliation":[{"name":"Laboratoire de Biodiversit\u00e9 et d\u2019Ecologie Microbienne (EA 3882), Universit\u00e9 de Brest, Technopole Brest Iroise, 29280 Plouzan\u00e9, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2016,3,10]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (2014). Antimicrobial Resistance: Global Report on Surveillance, World Health Organization."},{"key":"ref_2","unstructured":"Frieden, T. Antibiotic Resistance Threats in the United States, 2013, Available online: http:\/\/www.cdc.gov\/drugresistance\/pdf\/ar-threats-2013-508.pdf."},{"key":"ref_3","unstructured":"Monnet, D.L., and Albiger, B. European Centre for Disease Prevention and Control. Available online: http:\/\/ecdc.europa.eu\/en\/activities\/diseaseprogrammes\/ARHAI\/Pages\/about_programme.aspx."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.addr.2014.10.027","article-title":"Therapeutic strategies to combat antibiotic resistance","volume":"78","author":"Brooks","year":"2014","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1021\/np200906s","article-title":"Natural products as sources of new drugs over the 30 Years from 1981 to 2010","volume":"75","author":"Newman","year":"2012","journal-title":"J. Nat. Prod."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1038\/ja.2012.27","article-title":"Thoughts and facts about antibiotics: Where we are now and where we are heading","volume":"65","year":"2012","journal-title":"J. Antibiot. (Tokyo)"},{"key":"ref_7","first-page":"226","article-title":"On the antibacterial action of cultures of a penicillium, with special reference to their use in the isolation of B. influenzae","volume":"10","author":"Fleming","year":"1929","journal-title":"Br. J. Exp. Pathol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1346","DOI":"10.7164\/antibiotics.29.1346","article-title":"ML-236A, ML-236B, and ML-236C, new inhibitors of cholesterogensis produced by Penicillium citrinum","volume":"29","author":"Endo","year":"1976","journal-title":"J. Antibiot. (Tokyo)"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1111\/j.1749-6632.1993.tb17141.x","article-title":"Cyclosporins. Structure-activity relationships","volume":"696","author":"Fliri","year":"1993","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"544","DOI":"10.1111\/j.1751-7915.2010.00179.x","article-title":"Bioactive compounds from marine bacteria and fungi: Marine bioactive compounds","volume":"3","author":"Debbab","year":"2010","journal-title":"Microb. Biotechnol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1039\/C4NP00144C","article-title":"Marine natural products","volume":"32","author":"Blunt","year":"2015","journal-title":"Nat. Prod. Rep."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1039\/c0np00061b","article-title":"Secondary metabolites of fungi from marine habitats","volume":"28","author":"Rateb","year":"2011","journal-title":"Nat. Prod. Rep."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1370","DOI":"10.1038\/ismej.2013.250","article-title":"Microorganisms persist at record depths in the subseafloor of the Canterbury Basin","volume":"8","author":"Ciobanu","year":"2014","journal-title":"ISME J."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1146\/annurev.marine.010908.163708","article-title":"Marine chemical ecology: Chemical signals and cues structure marine populations, communities, and ecosystems","volume":"1","author":"Hay","year":"2009","journal-title":"Annu. Rev. Mar. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"3571","DOI":"10.1128\/AEM.04064-14","article-title":"Species richness and adaptation of marine fungi from deep-subseafloor sediments","volume":"81","author":"Navarri","year":"2015","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1007\/s10295-013-1366-3","article-title":"Strategies for mining fungal natural products","volume":"41","author":"Wiemann","year":"2014","journal-title":"J. Ind. Microbiol. Biotechnol."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"P\u00e9ter, G., and Rosa, C. (2006). Biodiversity and Ecophysiology of Yeasts, Springer-Verlag.","DOI":"10.1007\/3-540-30985-3"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1381","DOI":"10.1139\/W09-101","article-title":"Antimicrobial activity of endophytic fungi associated with Orchidaceae in Brazil","volume":"55","author":"Vaz","year":"2009","journal-title":"Can. J. Microbiol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/j.fm.2012.09.023","article-title":"Antifungal activity of Meyerozyma guilliermondii: Identification of active compounds synthesized during dough fermentation and their effect on long-term storage of wheat bread","volume":"33","author":"Coda","year":"2013","journal-title":"Food Microbiol."},{"key":"ref_20","first-page":"189","article-title":"Evaluation of secondary metabolites from mangrove associated fungi Meyerozyma guilliermondii","volume":"49","author":"Joel","year":"2013","journal-title":"Alex. J. Med."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1007\/s00248-012-0050-x","article-title":"Diversity and antimicrobial activity of culturable fungi isolated from six species of the South China Sea gorgonians","volume":"64","author":"Zhang","year":"2012","journal-title":"Microb. Ecol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1007\/s00284-013-0394-6","article-title":"Diverse deep-sea fungi from the South China Sea and their antimicrobial activity","volume":"67","author":"Zhang","year":"2013","journal-title":"Curr. Microbiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1007\/s11274-013-1418-x","article-title":"Diversity of cultivable fungi associated with Antarctic marine sponges and screening for their antimicrobial, antitumoral and antioxidant potential","volume":"30","author":"Vergara","year":"2014","journal-title":"World J. Microbiol. Biotechnol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1007\/s10126-014-9598-4","article-title":"Phylogenetic diversity and antibacterial activity of culturable fungi derived from the Zoanthid Palythoa haddoni in the South China Sea","volume":"17","author":"Qin","year":"2015","journal-title":"Mar. Biotechnol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1169","DOI":"10.1007\/s11274-013-1276-6","article-title":"Antimicrobial potential of deep surface sediment associated bacteria from the Sea of Japan","volume":"29","author":"Romanenko","year":"2013","journal-title":"World J. Microbiol. Biotechnol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1425","DOI":"10.1039\/C4NP00060A","article-title":"Fungal natural products in research and development","volume":"31","author":"Schueffler","year":"2014","journal-title":"Nat. Prod. Rep."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"999","DOI":"10.1039\/C3NP70118B","article-title":"Recent advances in deep-sea natural products","volume":"31","author":"Skropeta","year":"2014","journal-title":"Nat. Prod. Rep."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"3479","DOI":"10.3390\/md13063479","article-title":"Antibacterial and antifungal compounds from marine fungi","volume":"13","author":"Xu","year":"2015","journal-title":"Mar. Drugs"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"4650","DOI":"10.1016\/j.bmcl.2012.05.088","article-title":"Cristatumins A\u2013D, new indole alkaloids from the marine-derived endophytic fungus Eurotium cristatum EN-220","volume":"22","author":"Du","year":"2012","journal-title":"Bioorg. Med. Chem. Lett."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1016\/j.phytol.2012.07.010","article-title":"Eurocristatine, a new diketopiperazine dimer from the marine sponge-associated fungus Eurotium cristatum","volume":"5","author":"Gomes","year":"2012","journal-title":"Phytochem. Lett."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"528","DOI":"10.1042\/bj0430528","article-title":"Studies in the biochemistry of micro-organisms. 79. Fuscin, a metabolic product of Oidiodendron fuscum Robak. Part 1. Preparation, properties and antibacterial activity","volume":"43","author":"Michael","year":"1948","journal-title":"Biochem. J."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"753","DOI":"10.7164\/antibiotics.36.753","article-title":"Fermentation, isolation and characterization of antibiotic PR-1350","volume":"36","author":"Andersen","year":"1983","journal-title":"J. Antibiot. (Tokyo)"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"954","DOI":"10.1016\/j.cclet.2007.05.048","article-title":"Fusarielin E, a new antifungal antibiotic from Fusarium sp.","volume":"18","author":"Gai","year":"2007","journal-title":"Chin. Chem. Lett."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1066","DOI":"10.1002\/mrc.2293","article-title":"Structure elucidation of two new xanthone derivatives from the marine fungus Penicillium sp. (ZZF 32#) from the South China Sea","volume":"46","author":"Shao","year":"2008","journal-title":"Magn. Reson. Chem."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1100","DOI":"10.1248\/cpb.57.1100","article-title":"Lactone derivatives from the marine-derived fungus Penicillium sp. PSU-F44","volume":"57","author":"Trisuwan","year":"2009","journal-title":"Chem. Pharm. Bull. (Tokyo)"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2894","DOI":"10.1016\/j.bmcl.2011.03.076","article-title":"Penicisteroids A and B, antifungal and cytotoxic polyoxygenated steroids from the marine alga-derived endophytic fungus Penicillium chrysogenum QEN-24S","volume":"21","author":"Gao","year":"2011","journal-title":"Bioorg. Med. Chem. Lett."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1007\/s10482-014-0205-5","article-title":"Marine-derived Penicillium in Korea: Diversity, enzyme activity, and antifungal properties","volume":"106","author":"Park","year":"2014","journal-title":"Antonie van Leeuwenhoek"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1016\/S2222-1808(14)60650-2","article-title":"Antimicrobial constituents from endophytic fungus Fusarium sp.","volume":"5","author":"Hussain","year":"2015","journal-title":"Asian Pac. J. Trop. Dis."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1080\/10286020701273783","article-title":"Paeciloxanthone, a new cytotoxic xanthone from the marine mangrove fungus Paecilomyces sp. (Tree1-7)","volume":"10","author":"Wen","year":"2008","journal-title":"J. Asian Nat. Prod. Res."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1007\/s10600-013-0693-y","article-title":"Chemical components and bioactivity of the marine-derived fungus Paecilomyces sp. Collected from Tinggi Island, Malaysia","volume":"49","author":"Mosadeghzad","year":"2013","journal-title":"Chem. Nat. Compd."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"5343","DOI":"10.5897\/AJMR2013.6358","article-title":"Endophytic fungi from medicinal herb Salvia miltiorrhiza Bunge and their antimicrobial activity","volume":"7","author":"Lou","year":"2013","journal-title":"Afr. J. Microbiol. Res."},{"key":"ref_42","first-page":"988","article-title":"Isolation of and assay for cerulenin produced by sheath rot pathogen, Sarocladium oryzae (Saw.) Gams","volume":"55","author":"Sakthivel","year":"1986","journal-title":"Curr. Sci."},{"key":"ref_43","first-page":"251","article-title":"Isolation and phytotoxic effects of helvolic acid from plant pathogenic fungus Sarocladium oryzae","volume":"38","author":"Tschen","year":"1997","journal-title":"Bot. Bull. Acad. Sin."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"5503","DOI":"10.3390\/md12115503","article-title":"Inhibition of bacterial quorum sensing by extracts from aquatic fungi: First report from marine endophytes","volume":"12","author":"Reyes","year":"2014","journal-title":"Mar. Drugs"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1368","DOI":"10.3390\/md9081368","article-title":"Three new compounds from Aspergillus terreus PT06-2 grown in a high salt medium","volume":"9","author":"Wang","year":"2011","journal-title":"Mar. Drugs"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"2592","DOI":"10.1021\/np200707y","article-title":"Acremostrictin, a highly oxygenated metabolite from the marine fungus Acremonium strictum","volume":"74","author":"Julianti","year":"2011","journal-title":"J. Nat. Prod."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"234","DOI":"10.3390\/md10010234","article-title":"Antibacterial bisabolane-type sesquiterpenoids from the sponge-derived fungus Aspergillus sp.","volume":"10","author":"Li","year":"2012","journal-title":"Mar. Drugs"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1007\/s10295-014-1412-9","article-title":"Antifouling potentials of eight deep-sea-derived fungi from the South China Sea","volume":"41","author":"Zhang","year":"2014","journal-title":"J. Ind. Microbiol. Biotechnol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1016\/j.jbiosc.2013.09.017","article-title":"Characterization of antibacterial activity of bikaverin from Fusarium sp. HKF15","volume":"117","author":"Deshmukh","year":"2014","journal-title":"J. Biosci. Bioeng."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1515\/znb-2010-0611","article-title":"A cleistanthane diterpene from a marine-derived fusarium species under submerged fermentation","volume":"65","author":"Shiono","year":"2014","journal-title":"Z. F\u00fcr Naturforschung B"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1007\/PL00010971","article-title":"Recent studies on bacterial populations and processes in subseafloor sediments: A review","volume":"8","author":"Parkes","year":"2000","journal-title":"Hydrogeol. J."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"11651","DOI":"10.1073\/pnas.0811793106","article-title":"Subseafloor sedimentary life in the south Pacific Gyre","volume":"106","author":"Spivack","year":"2009","journal-title":"Proc. Natl. Acad. Sci."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"16213","DOI":"10.1073\/pnas.1203849109","article-title":"Global distribution of microbial abundance and biomass in subseafloor sediment","volume":"109","author":"Kallmeyer","year":"2012","journal-title":"Proc. Natl. Acad. Sci."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"2821","DOI":"10.1890\/0012-9658(2006)87[2821:CMCBMA]2.0.CO;2","article-title":"Chemically mediated competition between microbes and animals: Microbes as consumers in food webs","volume":"87","author":"Burkepile","year":"2006","journal-title":"Ecology"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1038\/ismej.2007.32","article-title":"Soaking it up: The complex lives of marine sponges and their microbial associates","volume":"1","author":"Taylor","year":"2007","journal-title":"ISME J."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1043","DOI":"10.1126\/science.1153527","article-title":"Microbial ecology of ocean biogeochemistry: A community perspective","volume":"320","author":"Strom","year":"2008","journal-title":"Science"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1007\/s10295-006-0126-z","article-title":"Production of fungal antibiotics using polymeric solid supports in solid-state and liquid fermentation","volume":"33","author":"Bigelis","year":"2006","journal-title":"J. Ind. Microbiol. Biotechnol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/j.mib.2005.04.006","article-title":"Solid-state fermentation\u2014Are there any biotechnological advantages?","volume":"8","author":"Lenz","year":"2005","journal-title":"Curr. Opin. Microbiol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1007\/s00253-008-1409-2","article-title":"Lovastatin biosynthetic genes of Aspergillus terreus are expressed differentially in solid-state and in liquid submerged fermentation","volume":"79","author":"Covarrubias","year":"2008","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1002\/1439-7633(20020703)3:7<619::AID-CBIC619>3.0.CO;2-9","article-title":"Big effects from small changes: Possible ways to explore nature\u2019s chemical diversity","volume":"3","author":"Bode","year":"2002","journal-title":"ChemBioChem"},{"key":"ref_61","unstructured":"Souchoth\u00e8que. Available online: http:\/\/www.univ-brest.fr\/souchotheque."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1439","DOI":"10.1016\/j.fsi.2013.03.357","article-title":"Antimicrobial peptides in oyster hemolymph: The bacterial connection","volume":"34","author":"Defer","year":"2013","journal-title":"Fish Shellfish Immunol."}],"container-title":["Marine Drugs"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1660-3397\/14\/3\/50\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T19:20:28Z","timestamp":1760210428000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1660-3397\/14\/3\/50"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,3,10]]},"references-count":62,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2016,3]]}},"alternative-id":["md14030050"],"URL":"https:\/\/doi.org\/10.3390\/md14030050","relation":{},"ISSN":["1660-3397"],"issn-type":[{"value":"1660-3397","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,3,10]]}}}