{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T02:16:18Z","timestamp":1773195378987,"version":"3.50.1"},"reference-count":44,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2017,2,28]],"date-time":"2017-02-28T00:00:00Z","timestamp":1488240000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Union\u2019s Seventh Framework Programme","award":["311848"],"award-info":[{"award-number":["311848"]}]},{"DOI":"10.13039\/501100004663","name":"MOST","doi-asserted-by":"publisher","award":["10705-3"],"award-info":[{"award-number":["10705-3"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>There is an urgent need for novel strategies to fight drug resistance and multi-drug resistance. As an alternative to the classic antibiotic therapy, attenuation of the bacteria virulence affecting their Quorum sensing (QS) system is a promising approach. Quorum sensing (QS) is a genetic regulation system that allows bacteria to communicate with each other and coordinate group behaviors. A new \u03b3-lactone that is capable of inhibiting the LasI\/R QS system, plakofuranolactone (1), was discovered in the extract of the marine sponge Plakortis cf. lita, and its structure, including absolute configuration, was determined by NMR spectroscopy, MS spectrometry, and quantum-mechanical prediction of optical rotation. The quorum quenching activity of plakofuranolactone was evaluated using reporter gene assays for long- and short-chain signals (E. coli pSB1075, E. coli pSB401, and C. violeaceum CV026) and was confirmed by measuring the total protease activity (a virulence factor which is under control of the LasI\/R system) of the wild-type P. aeruginosa PAO1. Further research will be pursued to assess the potential of plakofuranolactone as a new antivirulence lead compound and a chemical tool to increase the knowledge in this field.<\/jats:p>","DOI":"10.3390\/md15030059","type":"journal-article","created":{"date-parts":[[2017,2,28]],"date-time":"2017-02-28T10:57:52Z","timestamp":1488279472000},"page":"59","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Plakofuranolactone as a Quorum Quenching Agent from the Indonesian Sponge Plakortis cf. lita"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8723-9505","authenticated-orcid":false,"given":"Valeria","family":"Costantino","sequence":"first","affiliation":[{"name":"The Blue Chemistry Lab Group, Department of Pharmacy, University of Naples Federico II, 80131 Napoli, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8957-3259","authenticated-orcid":false,"given":"Gerardo","family":"Della Sala","sequence":"additional","affiliation":[{"name":"The Blue Chemistry Lab Group, Department of Pharmacy, University of Naples Federico II, 80131 Napoli, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7084-4204","authenticated-orcid":false,"given":"Kumar","family":"Saurav","sequence":"additional","affiliation":[{"name":"Department of Marine Biology, Leon H. Charney School of Marine Sciences, University of Haifa, Mt. Carmel, 31905 Haifa, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberta","family":"Teta","sequence":"additional","affiliation":[{"name":"The Blue Chemistry Lab Group, Department of Pharmacy, University of Naples Federico II, 80131 Napoli, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rinat","family":"Bar-Shalom","sequence":"additional","affiliation":[{"name":"Department of Marine Biology, Leon H. Charney School of Marine Sciences, University of Haifa, Mt. Carmel, 31905 Haifa, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3910-6518","authenticated-orcid":false,"given":"Alfonso","family":"Mangoni","sequence":"additional","affiliation":[{"name":"The Blue Chemistry Lab Group, Department of Pharmacy, University of Naples Federico II, 80131 Napoli, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Steindler","sequence":"additional","affiliation":[{"name":"Department of Marine Biology, Leon H. Charney School of Marine Sciences, University of Haifa, Mt. Carmel, 31905 Haifa, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2017,2,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1111\/j.1476-5381.2011.01250.x","article-title":"Novel classes of antibiotics or more of the same?","volume":"163","author":"Coates","year":"2011","journal-title":"Br. J. 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