{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T23:49:04Z","timestamp":1778975344011,"version":"3.51.4"},"reference-count":44,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2017,3,14]],"date-time":"2017-03-14T00:00:00Z","timestamp":1489449600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>Cancer development and progression are usually associated with glycosylation change, providing prognostic and diagnostic biomarkers, as well as therapeutic targets, for various cancers. In this work, Dicentrarchus labrax fucose binding lectin (DlFBL) and Haliotis discus discus sialic acid binding lectin (HddSBL) were genetically fused with soluble coxsackie-adenovirus receptor (sCAR), and produced through a bacterial expression system. Results showed that recombinant sCAR-DlFBL not only facilitated adenovirus Ad-EGFP infection in K562\/ADR and U87MG cells, but also enhanced the cytotoxicity of adenovirus harboring gene encoding Pinellia pedatisecta agglutinin (PPA) or DlFBL (Ad-PPA or Ad-DlFBL) on U87MG cells through inducing apoptosis. Recombinant sCAR-HddSBL facilitated Ad-EGFP infection, but dramatically counteracted the cytotoxicity of both Ad-PPA and Ad-DlFBL in U87MG cells. Further analysis revealed that sCAR-HddSBL, but not sCAR-DlFBL, significantly upregulated transcription factor E2F1 levels in U87MG cells, which might be responsible for the adverse effect of sCAR-HddSBL on Ad-PPA and Ad-DlFBL. Taken together, our data suggested that sCAR-DlFBL could be further developed to redirect therapeutic adenoviruses to infect cancer cells such as U87MG, and the sCAR-lectin fusion proteins for adenoviral retargeting should be carefully examined for possible survival signaling induced by lectins, such as HddSBL.<\/jats:p>","DOI":"10.3390\/md15030073","type":"journal-article","created":{"date-parts":[[2017,3,14]],"date-time":"2017-03-14T11:53:52Z","timestamp":1489492432000},"page":"73","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Marine Lectins DlFBL and HddSBL Fused with Soluble Coxsackie-Adenovirus Receptor Facilitate Adenovirus Infection in Cancer Cells BUT Have Different Effects on Cell Survival"],"prefix":"10.3390","volume":"15","author":[{"given":"Bingbing","family":"Wu","sequence":"first","affiliation":[{"name":"College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, Zhejiang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shengsheng","family":"Mei","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, Zhejiang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lianzhen","family":"Cui","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, Zhejiang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhenzhen","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, Zhejiang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianhong","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, Zhejiang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tao","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, Zhejiang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gongchu","family":"Li","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, Zhejiang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2017,3,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Ochoa-Alvarez, J.A., Krishnan, H., Shen, Y., Acharya, N.K., Han, M., McNulty, D.E., Hasegawa, H., Hyodo, T., Senga, T., and Geng, J.G. (2012). Plant lectin can target receptors containing sialic acid, exemplified by podoplanin, to inhibit transformed cell growth and migration. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0041845"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1002\/hep.21509","article-title":"Concanavalin a induces autophagy in hepatoma cells and has a therapeutic effect in a murine in situ hepatoma model","volume":"45","author":"Chang","year":"2007","journal-title":"Hepatology"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1093\/jb\/mvw057","article-title":"A novel l-fucose-binding lectin from Fenneropenaeus indicus induced cytotoxicity in breast cancer cells","volume":"161","author":"Chatterjee","year":"2016","journal-title":"J. Biochem."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"253","DOI":"10.4161\/auto.5.2.7561","article-title":"Molecular mechanisms of Polygonatum cyrtonema lectin-induced apoptosis and autophagy in cancer cells","volume":"5","author":"Liu","year":"2009","journal-title":"Autophagy"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"28344","DOI":"10.1038\/srep28344","article-title":"Crystal structure of mytilec, a galactose-binding lectin from the mussel mytilus galloprovincialis with cytotoxicity against certain cancer cell types","volume":"6","author":"Terada","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"7377","DOI":"10.3390\/md13127071","article-title":"Mytilec, a mussel r-type lectin, interacts with surface glycan gb3 on burkitt\u2019s lymphoma cells to trigger apoptosis through multiple pathways","volume":"13","author":"Hasan","year":"2015","journal-title":"Mar. Drugs"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"44772","DOI":"10.1074\/jbc.M112.418012","article-title":"A lectin from the mussel mytilus galloprovincialis has a highly novel primary structure and induces glycan-mediated cytotoxicity of globotriaosylceramide-expressing lymphoma cells","volume":"287","author":"Fujii","year":"2012","journal-title":"J. Biol. Chem."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Lu, Q., Li, N., Luo, J., Yu, M., Huang, Y., Wu, X., Wu, H., Liu, X.Y., and Li, G. (2012). Pinellia pedatisecta agglutinin interacts with the methylosome and induces cancer cell death. Oncogenesis, 1.","DOI":"10.1038\/oncsis.2012.30"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"4505","DOI":"10.1038\/srep04505","article-title":"Exogenous expression of marine lectins dlfbl and sprbl induces cancer cell apoptosis possibly through prmt5-e2f-1 pathway","volume":"4","author":"Wu","year":"2014","journal-title":"Sci. Rep."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"3994","DOI":"10.3390\/md12073994","article-title":"Adenovirus carrying gene encoding Haliotis discus discus sialic acid binding lectin induces cancer cell apoptosis","volume":"12","author":"Yang","year":"2014","journal-title":"Mar. Drugs"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1002\/jgm.2878","article-title":"Anguilla japonica lectin 1 delivery through adenovirus vector induces apoptotic cancer cell death through interaction with prmt5","volume":"18","author":"Li","year":"2016","journal-title":"J. Gene Med."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Li, G., Li, X., Wu, H., Yang, X., Zhang, Y., Chen, L., Wu, X., Cui, L., Wu, L., and Luo, J. (2014). Cd123 targeting oncolytic adenoviruses suppress acute myeloid leukemia cell proliferation in vitro and in vivo. Blood Cancer J., 4.","DOI":"10.1038\/bcj.2014.15"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"35478","DOI":"10.18632\/oncotarget.8155","article-title":"Hallmarks of glycosylation in cancer","volume":"7","author":"Munkley","year":"2016","journal-title":"Oncotarget"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1060","DOI":"10.1093\/glycob\/cwr045","article-title":"Lectin microarray profiling of metastatic breast cancers","volume":"21","author":"Fry","year":"2011","journal-title":"Glycobiology"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2508","DOI":"10.1021\/pr201206w","article-title":"Lectin chromatography\/mass spectrometry discovery workflow identifies putative biomarkers of aggressive breast cancers","volume":"11","author":"Drake","year":"2012","journal-title":"J. Proteome Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"4541","DOI":"10.1021\/pr300330z","article-title":"Identification and confirmation of differentially expressed fucosylated glycoproteins in the serum of ovarian cancer patients using a lectin array and lc-ms\/ms","volume":"11","author":"Wu","year":"2012","journal-title":"J. Proteome Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1021\/pr8007013","article-title":"Pancreatic cancer serum detection using a lectin\/glyco-antibody array method","volume":"8","author":"Li","year":"2009","journal-title":"J. Proteome Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"djw038","DOI":"10.1093\/jnci\/djw038","article-title":"Relationship between increased fucosylation and metastatic potential in colorectal cancer","volume":"108","author":"Osuga","year":"2016","journal-title":"J. Natl. Cancer Inst."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"68","DOI":"10.4149\/neo_2009_01_68","article-title":"Clinical utility of the interaction between lectin and serum prostate specific antigen in prostate cancer","volume":"56","author":"Batabyal","year":"2009","journal-title":"Neoplasma"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"5507","DOI":"10.1016\/j.jprot.2012.06.027","article-title":"A lectin-coupled, targeted proteomic mass spectrometry (mrm ms) platform for identification of multiple liver cancer biomarkers in human plasma","volume":"75","author":"Ahn","year":"2012","journal-title":"J. Proteom."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"3126","DOI":"10.1002\/pmic.201600064","article-title":"Development and application of a novel recombinant aleuria aurantia lectin with enhanced core fucose binding for identification of glycoprotein biomarkers of hepatocellular carcinoma","volume":"16","author":"Norton","year":"2016","journal-title":"Proteomics"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1038\/nrurol.2016.65","article-title":"The role of glycans in the development and progression of prostate cancer","volume":"13","author":"Munkley","year":"2016","journal-title":"Nat. Rev. Urol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"3073","DOI":"10.1002\/pmic.201500472","article-title":"A glycoproteomic approach to identify novel glycomarkers for cancer stem cells","volume":"16","author":"Sawanobori","year":"2016","journal-title":"Proteomics"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1053\/j.gastro.2016.09.004","article-title":"Fucosylation deficiency in mice leads to colitis and adenocarcinoma","volume":"152","author":"Wang","year":"2017","journal-title":"Gastroenterology"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Zhao, Y.P., Xu, X.Y., Fang, M., Wang, H., You, Q., Yi, C.H., Ji, J., Gu, X., Zhou, P.T., and Cheng, C. (2014). Decreased core-fucosylation contributes to malignancy in gastric cancer. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0094536"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"10304","DOI":"10.1073\/pnas.1608069113","article-title":"Precision glycocalyx editing as a strategy for cancer immunotherapy","volume":"113","author":"Xiao","year":"2016","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"837","DOI":"10.3892\/ol.2014.2201","article-title":"Pinellia pedatisecta agglutinin-based lectin blot analysis distinguishes between glycosylation patterns in various cancer cell lines","volume":"8","author":"Li","year":"2014","journal-title":"Oncol. Lett."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1007\/s12185-009-0308-3","article-title":"Mannose-exposing myeloid leukemia cells detected by the scar-ppa fusion protein","volume":"89","author":"Li","year":"2009","journal-title":"Int. J. Hematol."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Chen, K., Yang, X., Wu, L., Yu, M., Li, X., Li, N., Wang, S., and Li, G. (2013). Pinellia pedatisecta agglutinin targets drug resistant k562\/adr leukemia cells through binding with sarcolemmal membrane associated protein and enhancing macrophage phagocytosis. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0074363"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"879","DOI":"10.1038\/sj.cr.7310108","article-title":"Targeting gene-virotherapy of cancer and its prosperity","volume":"16","author":"Liu","year":"2006","journal-title":"Cell Res."},{"key":"ref_31","first-page":"3395","article-title":"The development of conditionally replicative adenoviruses for cancer therapy","volume":"6","author":"Curiel","year":"2000","journal-title":"Clin. Cancer Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/S1470-2045(01)00618-0","article-title":"Oncolytic biotherapy: A novel therapeutic plafform","volume":"3","author":"Hawkins","year":"2002","journal-title":"Lancet Oncol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1038\/89901","article-title":"Replication-selective virotherapy for cancer: Biological principles, risk management and future directions","volume":"7","author":"Kirn","year":"2001","journal-title":"Nat. Med."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1579","DOI":"10.1126\/science.286.5444.1579","article-title":"Structural analysis of the mechanism of adenovirus binding to its human cellular receptor, car","volume":"286","author":"Bewley","year":"1999","journal-title":"Science"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1320","DOI":"10.1126\/science.275.5304.1320","article-title":"Isolation of a common receptor for coxsackie b viruses and adenoviruses 2 and 5","volume":"275","author":"Bergelson","year":"1997","journal-title":"Science"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1038\/sj.leu.2405034","article-title":"Enhanced antitumor activity by a selective conditionally replicating adenovirus combining with mda-7\/interleukin-24 for b-lymphoblastic leukemia via induction of apoptosis","volume":"22","author":"Qian","year":"2008","journal-title":"Leukemia"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"43496","DOI":"10.18632\/oncotarget.6292","article-title":"Cd47-retargeted oncolytic adenovirus armed with melanoma differentiation-associated gene-7\/interleukin-24 suppresses in vivo leukemia cell growth","volume":"6","author":"Li","year":"2015","journal-title":"Oncotarget"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1038\/ncb0703-587","article-title":"The yin and yang of e2f-1: Balancing life and death","volume":"5","year":"2003","journal-title":"Nat. Cell Biol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1182\/blood.V97.1.227","article-title":"Overexpression of e2f-1 leads to cytokine-independent proliferation and survival in the hematopoietic cell line baf-b03","volume":"97","author":"Gala","year":"2001","journal-title":"Blood"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"3602","DOI":"10.1073\/pnas.91.9.3602","article-title":"P53 and e2f-1 cooperate to mediate apoptosis","volume":"91","author":"Wu","year":"1994","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1038\/35036614","article-title":"Role for the p53 homologue p73 in e2f-1-induced apoptosis","volume":"407","author":"Irwin","year":"2000","journal-title":"Nature"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1038\/ncb974","article-title":"Chk2 activates e2f-1 in response to DNA damage","volume":"5","author":"Stevens","year":"2003","journal-title":"Nat. Cell Biol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1785","DOI":"10.1038\/emboj.2012.17","article-title":"Arginine methylation controls growth regulation by e2f-1","volume":"31","author":"Cho","year":"2012","journal-title":"EMBO J."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.molcel.2013.08.039","article-title":"Arginine methylation-dependent reader-writer interplay governs growth control by e2f-1","volume":"52","author":"Zheng","year":"2013","journal-title":"Mol. Cell"}],"container-title":["Marine Drugs"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1660-3397\/15\/3\/73\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T18:30:25Z","timestamp":1760207425000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1660-3397\/15\/3\/73"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,3,14]]},"references-count":44,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2017,3]]}},"alternative-id":["md15030073"],"URL":"https:\/\/doi.org\/10.3390\/md15030073","relation":{},"ISSN":["1660-3397"],"issn-type":[{"value":"1660-3397","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,3,14]]}}}