{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T06:41:05Z","timestamp":1790145665416,"version":"4.0.1"},"reference-count":43,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2017,4,10]],"date-time":"2017-04-10T00:00:00Z","timestamp":1491782400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31571779"],"award-info":[{"award-number":["31571779"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"High &amp; New project of Fujian Marine Fisheries Department","award":["[2015]20"],"award-info":[{"award-number":["[2015]20"]}]},{"name":"Fujian Production &amp; Study project of Provincial Science &amp; Technology Hall","award":["2016N5006"],"award-info":[{"award-number":["2016N5006"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>Schizochytrium protein hydrolysate (SPH) was prepared through stepwise enzymatic hydrolysis by alcalase and flavourzyme sequentially. The proportion of hydrophobic amino acids of SPH was 34.71%. The molecular weight (MW) of SPH was principally concentrated at 180\u20133000 Da (52.29%). SPH was divided into two fractions by ultrafiltration: SPH-I (MW &lt; 3 kDa) and SPH-II (MW &gt; 3 kDa). Besides showing lipid peroxidation inhibitory activity in vitro, SPH-I exhibited high DPPH and ABTS radicals scavenging activities with IC50 of 350 \u03bcg\/mL and 17.5 \u03bcg\/mL, respectively. In addition, the antioxidant activity of SPH-I was estimated in vivo using the model of acute alcohol-induced liver injury in mice. For the hepatoprotective effects, oral administration of SPH-I at different concentrations (100, 300 mg\/kg BW) to the mice subjected to alcohol significantly decreased serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities and hepatic malondialdehyde (MDA) level compared to the untreated mice. Besides, SPH-I could effectively restore the hepatic superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities and glutathione (GSH) level. Results suggested that SPH was rich in biopeptides that could be exploited as antioxidant molecules against oxidative stress in human body.<\/jats:p>","DOI":"10.3390\/md15040115","type":"journal-article","created":{"date-parts":[[2017,4,13]],"date-time":"2017-04-13T02:39:17Z","timestamp":1492051157000},"page":"115","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":43,"title":["In Vitro Antioxidant Activities of Enzymatic Hydrolysate from Schizochytrium sp. and Its Hepatoprotective Effects on Acute Alcohol-Induced Liver Injury In Vivo"],"prefix":"10.3390","volume":"15","author":[{"given":"Xixi","family":"Cai","sequence":"first","affiliation":[{"name":"The Key Lab of Analysis and Detection Technology for Food Safety of the MOE, College of Chemistry, Fuzhou University, Fuzhou 350108, China"},{"name":"College of Biological Science and Technology, Fuzhou University, Fuzhou 350108, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Yan","sequence":"additional","affiliation":[{"name":"College of Biological Science and Technology, Fuzhou University, Fuzhou 350108, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nanyan","family":"Fu","sequence":"additional","affiliation":[{"name":"The Key Lab of Analysis and Detection Technology for Food Safety of the MOE, College of Chemistry, Fuzhou University, Fuzhou 350108, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaoyun","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Biological Science and Technology, Fuzhou University, Fuzhou 350108, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2017,4,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3103","DOI":"10.1016\/j.procbio.2005.03.007","article-title":"Effect of culture conditions on docosahexaenoic acid production by Schizochytrium sp. 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