{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T13:09:55Z","timestamp":1792069795169,"version":"4.4.0"},"reference-count":21,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2017,5,19]],"date-time":"2017-05-19T00:00:00Z","timestamp":1495152000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006382","name":"Universidad de Oviedo","doi-asserted-by":"publisher","award":["UNOV-11-MA-02"],"award-info":[{"award-number":["UNOV-11-MA-02"]}],"id":[{"id":"10.13039\/501100006382","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100011941","name":"Gobierno del Principado de Asturias","doi-asserted-by":"publisher","award":["SV-PA-13-ECOEMP-62"],"award-info":[{"award-number":["SV-PA-13-ECOEMP-62"]}],"id":[{"id":"10.13039\/100011941","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100011941","name":"Gobierno del Principado de Asturias","doi-asserted-by":"publisher","award":["IDI\/2016\/000410"],"award-info":[{"award-number":["IDI\/2016\/000410"]}],"id":[{"id":"10.13039\/100011941","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004837","name":"Ministerio de Ciencia e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["PCT-010000-2010-4"],"award-info":[{"award-number":["PCT-010000-2010-4"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004837","name":"Ministerio de Ciencia e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["INP-2011-0016-PCT-010000 ACT6"],"award-info":[{"award-number":["INP-2011-0016-PCT-010000 ACT6"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>The present article describes the isolation of a new natural product of the lobophorin family, designated as lobophorin K (1), from cultures of the marine actinobacteria Streptomyces sp. M-207, previously isolated from the cold-water coral Lophelia pertusa collected at 1800 m depth during an expedition to the submarine Avil\u00e9s Canyon. Its structure was determined using a combination of spectroscopic techniques, mainly ESI-TOF MS and 1D and 2D NMR. This new natural product displayed cytotoxic activity against two human tumor cell lines, such as pancreatic carcinoma (MiaPaca-2) and breast adenocarcinoma (MCF-7). Lobophorin K also displayed moderate and selective antibiotic activity against pathogenic Gram-positive bacteria such as Staphylococcus aureus.<\/jats:p>","DOI":"10.3390\/md15050144","type":"journal-article","created":{"date-parts":[[2017,5,23]],"date-time":"2017-05-23T01:47:33Z","timestamp":1495504053000},"page":"144","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":66,"title":["Lobophorin K, a New Natural Product with Cytotoxic Activity Produced by Streptomyces sp. M-207 Associated with the Deep-Sea Coral Lophelia pertusa"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3277-9085","authenticated-orcid":false,"given":"Alfredo","family":"Bra\u00f1a","sequence":"first","affiliation":[{"name":"Departamento de Biolog\u00eda Funcional, \u00c1rea de Microbiolog\u00eda, Instituto Universitario de Oncolog\u00eda del Principado de Asturias, Universidad de Oviedo, 33006 Oviedo, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aida","family":"Sarmiento-Vizca\u00edno","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Funcional, \u00c1rea de Microbiolog\u00eda, Instituto Universitario de Oncolog\u00eda del Principado de Asturias, Universidad de Oviedo, 33006 Oviedo, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel","family":"Osset","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Funcional, \u00c1rea de Microbiolog\u00eda, Instituto Universitario de Oncolog\u00eda del Principado de Asturias, Universidad de Oviedo, 33006 Oviedo, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4556-688X","authenticated-orcid":false,"given":"Ignacio","family":"P\u00e9rez-Victoria","sequence":"additional","affiliation":[{"name":"Fundaci\u00f3n MEDINA, Centro de Excelencia en Investigaci\u00f3n de Medicamentos Innovadores en Andaluc\u00eda, Avda. del Conocimiento 3, Parque Tecnol\u00f3gico de Ciencias de la Salud, E-18016 Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jes\u00fas","family":"Mart\u00edn","sequence":"additional","affiliation":[{"name":"Fundaci\u00f3n MEDINA, Centro de Excelencia en Investigaci\u00f3n de Medicamentos Innovadores en Andaluc\u00eda, Avda. del Conocimiento 3, Parque Tecnol\u00f3gico de Ciencias de la Salud, E-18016 Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nuria","family":"De Pedro","sequence":"additional","affiliation":[{"name":"Fundaci\u00f3n MEDINA, Centro de Excelencia en Investigaci\u00f3n de Medicamentos Innovadores en Andaluc\u00eda, Avda. del Conocimiento 3, Parque Tecnol\u00f3gico de Ciencias de la Salud, E-18016 Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2389-8576","authenticated-orcid":false,"given":"Mercedes","family":"De la Cruz","sequence":"additional","affiliation":[{"name":"Fundaci\u00f3n MEDINA, Centro de Excelencia en Investigaci\u00f3n de Medicamentos Innovadores en Andaluc\u00eda, Avda. del Conocimiento 3, Parque Tecnol\u00f3gico de Ciencias de la Salud, E-18016 Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caridad","family":"D\u00edaz","sequence":"additional","affiliation":[{"name":"Fundaci\u00f3n MEDINA, Centro de Excelencia en Investigaci\u00f3n de Medicamentos Innovadores en Andaluc\u00eda, Avda. del Conocimiento 3, Parque Tecnol\u00f3gico de Ciencias de la Salud, E-18016 Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisca","family":"Vicente","sequence":"additional","affiliation":[{"name":"Fundaci\u00f3n MEDINA, Centro de Excelencia en Investigaci\u00f3n de Medicamentos Innovadores en Andaluc\u00eda, Avda. del Conocimiento 3, Parque Tecnol\u00f3gico de Ciencias de la Salud, E-18016 Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1607-5106","authenticated-orcid":false,"given":"Fernando","family":"Reyes","sequence":"additional","affiliation":[{"name":"Fundaci\u00f3n MEDINA, Centro de Excelencia en Investigaci\u00f3n de Medicamentos Innovadores en Andaluc\u00eda, Avda. del Conocimiento 3, Parque Tecnol\u00f3gico de Ciencias de la Salud, E-18016 Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3233-3582","authenticated-orcid":false,"given":"Luis","family":"Garc\u00eda","sequence":"additional","affiliation":[{"name":"Departamento de Ingenier\u00eda Qu\u00edmica y Tecnolog\u00eda del Medio Ambiente, \u00c1rea de Ingenier\u00eda Qu\u00edmica, Universidad de Oviedo, 33006 Oviedo, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gloria","family":"Blanco","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Funcional, \u00c1rea de Microbiolog\u00eda, Instituto Universitario de Oncolog\u00eda del Principado de Asturias, Universidad de Oviedo, 33006 Oviedo, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2017,5,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2003","DOI":"10.1016\/S0960-894X(99)00337-6","article-title":"Lobophorins A and B, new antiinflammatory macrolides produced by a tropical marine bacterium","volume":"19","author":"Jiang","year":"1999","journal-title":"Bioorg. Med. Chem. Lett."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1038\/ja.2011.78","article-title":"Lobophorins E and F, new spirotetronate antibiotics from a South China Sea-derived Streptomyces sp. SCSIO 01127","volume":"64","author":"Niu","year":"2011","journal-title":"J. Antibiot."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"3885","DOI":"10.1007\/s00253-012-4681-0","article-title":"Three antimycobacterial metabolites identified from a marine-derived Streptomyces sp. MS100061","volume":"97","author":"Chen","year":"2013","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"3891","DOI":"10.3390\/md11103891","article-title":"New spirotetronate antibiotics, lobophorins H and I, from a South China Sea-derived Streptomyces sp. 12A35","volume":"11","author":"Pan","year":"2013","journal-title":"Mar. Drugs"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1038\/ja.2013.115","article-title":"Structure and activity of lobophorins from a turrid mollusk-associated Streptomyces sp.","volume":"67","author":"Lin","year":"2014","journal-title":"J. Antibiot."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"877","DOI":"10.1021\/acsmedchemlett.5b00127","article-title":"Novel Lobophorins Inhibit Oral Cancer Cell Growth and Induce Atf4- and Chop-Dependent Cell Death in Murine Fibroblasts","volume":"6","author":"Cruz","year":"2015","journal-title":"ACS Med. Chem. Lett."},{"key":"ref_7","first-page":"721","article-title":"Isolation and identification of a new antibiotic, lobophorin J, from a deep sea-derived Streptomyces sp. 12A35","volume":"40","author":"Song","year":"2015","journal-title":"Chin. J. Antibiot."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"359","DOI":"10.3390\/md9030359","article-title":"Lobophorin C and D, new kijanimicin derivatives from a marine sponge-associated actinomycetal strain AZS17","volume":"9","author":"Wei","year":"2011","journal-title":"Mar. Drugs"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"512","DOI":"10.1007\/s00248-014-0508-0","article-title":"Two Streptomyces Species Producing Antibiotic, Antitumor, and Anti Inflammatory Compounds Are Widespread Among Intertidal Macroalgae and Deep Sea Coral Reef Invertebrates from the Central Cantabrian Sea","volume":"69","author":"Fiedler","year":"2015","journal-title":"Microb. Ecol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1328","DOI":"10.1099\/ijs.0.000107","article-title":"Myceligenerans cantabricum sp. nov., a barotolerant actinobacterium isolated from a deep cold water coral","volume":"65","author":"Molina","year":"2015","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1007\/s00248-015-0654-z","article-title":"Atmospheric Dispersal of Bioactive Streptomyces albidoflavus Strains Among Terrestrial and Marine Environments","volume":"71","author":"Nava","year":"2016","journal-title":"Microb. Ecol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1007\/s00248-016-0845-2","article-title":"Pharmacological potential of phylogenetically diverse Actinobacteria isolated from deep-sea coral ecosystems of the submarine Avil\u00e9s Canyon in the Cantabrian Sea","volume":"73","author":"Palacios","year":"2017","journal-title":"Microb. Ecol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1055\/s-0042-101763","article-title":"Combined LC\/UV\/MS and NMR Strategies for the Dereplication of Marine Natural Products","volume":"82","author":"Reyes","year":"2016","journal-title":"Planta Med."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2725","DOI":"10.1093\/molbev\/mst197","article-title":"MEGA6: Molecular Evolutionary Genetics Analysis version 6.0","volume":"30","author":"Tamura","year":"2013","journal-title":"Mol. Biol. Evol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1038\/msb.2011.75","article-title":"Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega","volume":"7","author":"Sievers","year":"2011","journal-title":"Mol. Syst. Biol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1007\/BF01731581","article-title":"A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences","volume":"16","author":"Kimura","year":"1980","journal-title":"J. Mol. Evol."},{"key":"ref_17","first-page":"406","article-title":"The neighbor-joining method: a new method for reconstructing phylogenetic trees","volume":"4","author":"Saitou","year":"1987","journal-title":"Mol. Biol. Evol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"783","DOI":"10.2307\/2408678","article-title":"Conference limits on phylogenies: an approach using the bootstrap","volume":"39","author":"Felsenstein","year":"1985","journal-title":"Evolution"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1007\/s00203-014-0977-z","article-title":"Activation and silencing of secondary metabolites in Streptomyces albus and Streptomyces lividans after transformation with cosmids containing the thienamycin gene cluster from Streptomyces cattleya","volume":"196","author":"Rohr","year":"2014","journal-title":"Arch. Microbiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1477","DOI":"10.3390\/md11051477","article-title":"Balibalosides, an Original Family of Glucosylated Sesterterpenes Produced by the Mediterranean Sponge Oscarella balibaloi","volume":"11","author":"Audoin","year":"2013","journal-title":"Mar. Drugs"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1177\/108705719900400206","article-title":"A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening Assays","volume":"4","author":"Zhang","year":"1999","journal-title":"Biomol. Screen."}],"container-title":["Marine Drugs"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1660-3397\/15\/5\/144\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T18:36:18Z","timestamp":1760207778000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1660-3397\/15\/5\/144"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,5,19]]},"references-count":21,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2017,5]]}},"alternative-id":["md15050144"],"URL":"https:\/\/doi.org\/10.3390\/md15050144","relation":{},"ISSN":["1660-3397"],"issn-type":[{"value":"1660-3397","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,5,19]]}}}