{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T22:35:37Z","timestamp":1792794937848,"version":"4.5.0"},"reference-count":40,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2017,10,10]],"date-time":"2017-10-10T00:00:00Z","timestamp":1507593600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>Fish discards are of major concern in new EU policies. Alternatives for the management of the new biomass that has to be landed is compulsory. The production of bioactive compounds from fish protein hydrolysates (FPH) has been explored in recent years. However, the viability of Scyliorhinus canicula discards, which might account for up to 90\u2013100% of captures in mixed trawler, gillnet, and longline industrial fisheries, to produce FPH from the muscle with bioactivities has still not been studied in terms of the optimization of the experimental conditions to enhance its production. The effect of pH and temperature on the hydrolysis of the S. canicula muscle was mediated by three commercial proteases using response surface methodology. Temperatures of 64.6 \u00b0C and 60.8 \u00b0C and pHs of 9.40 and 8.90 were established as the best hydrolysis conditions for Alcalase and Esperase, respectively. Optimization of the best conditions for the maximization of antihypertensive and antioxidant activities was performed. Higher Angiotensin-converting enzyme (ACE) activity was found with Esperase. The pH optimum and temperature optimum for antioxidants were 55 \u00b0C\/pH8.0 for ABTS\/DPPH-Esperase, 63.1 \u00b0C\/pH9.0 for DPPH-Alcalase, and 55 \u00b0C\/pH9.0 for ABTS-Alcalase. No hydrolysis was detected when using Protamex.<\/jats:p>","DOI":"10.3390\/md15100306","type":"journal-article","created":{"date-parts":[[2017,10,10]],"date-time":"2017-10-10T10:34:29Z","timestamp":1507631669000},"page":"306","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":61,"title":["Production of Fish Protein Hydrolysates from Scyliorhinus canicula Discards with Antihypertensive and Antioxidant Activities by Enzymatic Hydrolysis and Mathematical Optimization Using Response Surface Methodology"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1122-4726","authenticated-orcid":false,"given":"Jos\u00e9","family":"V\u00e1zquez","sequence":"first","affiliation":[{"name":"Grupo de Reciclado y Valorizaci\u00f3n de Materiales Residuales (REVAL), Instituto de Investigaci\u00f3ns Mari\u00f1as (IIM-CSIC), r\/Eduardo Cabello, 6, Vigo-36208 Galicia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Blanco","sequence":"additional","affiliation":[{"name":"Grupo de Bioqu\u00edmica de Alimentos, Instituto de Investigaci\u00f3ns Mari\u00f1as (IIM-CSIC), r\/Eduardo Cabello, 6, Vigo-36208 Galicia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agueda","family":"Massa","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Investigaci\u00f3n y Desarrollo Pesquero (INIDEP), Paseo Victoria Ocampo N\u00b01 Escollera Norte, C.C.175 - 7600 Mar del Plata, Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Rivadavia 1917, 1033 Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabel","family":"Amado","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica Anal\u00edtica y Alimentaria, Facultade de Ciencias de Ourense, Universidade de Vigo, Campus As Lagoas s\/n, 32004 Ourense, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4918-9399","authenticated-orcid":false,"given":"Ricardo","family":"P\u00e9rez-Mart\u00edn","sequence":"additional","affiliation":[{"name":"Grupo de Bioqu\u00edmica de Alimentos, Instituto de Investigaci\u00f3ns Mari\u00f1as (IIM-CSIC), r\/Eduardo Cabello, 6, Vigo-36208 Galicia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2017,10,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"375","DOI":"10.2960\/J.v35.m490","article-title":"Overview of continental shelf elasmobranch fisheries in the Cantabrian Sea","volume":"35","author":"Olaso","year":"2005","journal-title":"J. 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