{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T03:53:00Z","timestamp":1782186780477,"version":"3.54.5"},"reference-count":42,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2019,2,1]],"date-time":"2019-02-01T00:00:00Z","timestamp":1548979200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key Research and Development Program of China (2017YFC0306600, 2018YFC0309804), Hundred Talents Program of CAS (SIDSSE\u2013BR\u2013201401).","award":["2017YFC0306600, 2018YFC0309804\uff0cSIDSSE\u2013BR\u2013201401"],"award-info":[{"award-number":["2017YFC0306600, 2018YFC0309804\uff0cSIDSSE\u2013BR\u2013201401"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>A novel, cold-adapted, and acid-base stable manganese superoxide dismutase (Ps-Mn-SOD) was cloned from hadal sea cucumber Paelopatides sp. The dimeric recombinant enzyme exhibited approximately 60 kDa in molecular weight, expressed activity from 0 \u00b0C to 70 \u00b0C with an optimal temperature of 0 \u00b0C, and resisted wide pH values from 2.2\u201313.0 with optimal activity (&gt; 70%) at pH 5.0\u201312.0. The Km and Vmax of Ps-Mn-SOD were 0.0329 \u00b1 0.0040 mM and 9112 \u00b1 248 U\/mg, respectively. At tested conditions, Ps-Mn-SOD was relatively stable in divalent metal ion and other chemicals, such as \u03b2-mercaptoethanol, dithiothreitol, Tween 20, Triton X-100, and Chaps. Furthermore, the enzyme showed striking stability in 5 M urea or 4 M guanidine hydrochloride, resisted digestion by proteases, and tolerated a high hydrostatic pressure of 100 MPa. The resistance of Ps-Mn-SOD against low temperature, extreme acidity and alkalinity, chemicals, proteases, and high pressure make it a potential candidate in biopharmaceutical and nutraceutical fields.<\/jats:p>","DOI":"10.3390\/md17020084","type":"journal-article","created":{"date-parts":[[2019,2,1]],"date-time":"2019-02-01T03:08:05Z","timestamp":1548990485000},"page":"84","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Characteristics of a Novel Manganese Superoxide Dismutase of a Hadal Sea Cucumber (Paelopatides sp.) from the Mariana Trench"],"prefix":"10.3390","volume":"17","author":[{"given":"Yanan","family":"Li","sequence":"first","affiliation":[{"name":"Institute of Deep-Sea Science and Engineering, Chinese Academy of Sciences, Sanya 572000, China"},{"name":"College of Earth and Planetary Sciences, University of Chinese Academy of Sciences, Beijing 100039, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue","family":"Kong","sequence":"additional","affiliation":[{"name":"Institute of Deep-Sea Science and Engineering, Chinese Academy of Sciences, Sanya 572000, China"},{"name":"College of Earth and Planetary Sciences, University of Chinese Academy of Sciences, Beijing 100039, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haibin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Deep-Sea Science and Engineering, Chinese Academy of Sciences, Sanya 572000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,2,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1111\/j.1399-3054.2009.01326.x","article-title":"ROS in biotic interactions","volume":"138","author":"Torres","year":"2010","journal-title":"Physiol. 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