{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T10:59:53Z","timestamp":1776077993336,"version":"3.50.1"},"reference-count":31,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2019,2,1]],"date-time":"2019-02-01T00:00:00Z","timestamp":1548979200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Government of New Caledonia","award":["Thesis Grant"],"award-info":[{"award-number":["Thesis Grant"]}]},{"DOI":"10.13039\/501100006364","name":"Institut National Du Cancer","doi-asserted-by":"publisher","award":["NECROTRAIL"],"award-info":[{"award-number":["NECROTRAIL"]}],"id":[{"id":"10.13039\/501100006364","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006364","name":"Institut National Du Cancer","doi-asserted-by":"publisher","award":["Marine Natural Products"],"award-info":[{"award-number":["Marine Natural Products"]}],"id":[{"id":"10.13039\/501100006364","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004099","name":"Ligue Contre le Cancer","doi-asserted-by":"publisher","award":["CSIRGO"],"award-info":[{"award-number":["CSIRGO"]}],"id":[{"id":"10.13039\/501100004099","id-type":"DOI","asserted-by":"publisher"}]},{"name":"European Union\u2019s Horizon 2020 research and innovation programme","award":["654008"],"award-info":[{"award-number":["654008"]}]},{"name":"ANR\/Investissements d\u2019Avenir program via the OCEANOMICs project","award":["ANR-11-BTBR-0008"],"award-info":[{"award-number":["ANR-11-BTBR-0008"]}]},{"DOI":"10.13039\/501100006331","name":"Canc\u00e9rop\u00f4le PACA","doi-asserted-by":"publisher","award":["Marine Natural Products"],"award-info":[{"award-number":["Marine Natural Products"]}],"id":[{"id":"10.13039\/501100006331","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011697","name":"R\u00e9gion Bretagne","doi-asserted-by":"publisher","award":["ARED Fellowship program"],"award-info":[{"award-number":["ARED Fellowship program"]}],"id":[{"id":"10.13039\/501100011697","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>The marine \u03b1-pyrone macrolide neurymenolide A was previously isolated from the Fijian red macroalga, Neurymenia fraxinifolia, and characterized as an antibacterial agent against antibiotic-resistant strains that also exhibited moderate cytotoxicity in vitro against cancer cell lines. This compound was also shown to exhibit allelopathic effects on Scleractinian corals. However, to date no mechanism of action has been described in the literature. The present study showed, for the first time, the isolation of neurymenolide A from the New Caledonian Rhodophyta, Phacelocarpus neurymenioides. We confirmed the compound\u2019s moderate cytotoxicity in vitro against several human cell lines, including solid and hematological malignancies. Furthermore, we combined fluorescence microscopy and flow cytometry to demonstrate that treatment of U-2 OS osteosarcoma human cells with neurymenolide A could block cell division in prometaphase by inhibiting the correct formation of the mitotic spindle, which induced a mitotic catastrophe that led to necrosis and apoptosis. Absolute configuration of the stereogenic center C-17 of neurymenolide A was deduced by comparison of the experimental and theoretical circular dichroism spectra. Since the total synthesis of this compound has already been described, our findings open new avenues in cancer treatment for this class of marine molecules, including a new source for the natural product.<\/jats:p>","DOI":"10.3390\/md17020093","type":"journal-article","created":{"date-parts":[[2019,2,1]],"date-time":"2019-02-01T11:19:58Z","timestamp":1549019998000},"page":"93","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Neurymenolide A, a Novel Mitotic Spindle Poison from the New Caledonian Rhodophyta Phacelocarpus neurymenioides"],"prefix":"10.3390","volume":"17","author":[{"given":"Sofia-El\u00e9na","family":"Motuhi","sequence":"first","affiliation":[{"name":"UMR ENTROPIE (IRD\u2014Universit\u00e9 de La R\u00e9union\u2014CNRS), Laboratoire d\u2019Excellence Labex-CORAIL, Institut de Recherche pour le D\u00e9veloppement (IRD), BP A5, 98848 Noum\u00e9a CEDEX, New Caledonia, France"},{"name":"Sorbonne Universit\u00e9, CNRS, USR 3151, Protein Phosphorylation &amp; Human Diseases, Station Biologique de Roscoff, CS 90074, 29688 Roscoff CEDEX, France"},{"name":"UMR 7272 CNRS, Marine Natural Products Team, Nice Institute of Chemistry (ICN), University Nice Sophia Antipolis, Parc Valrose, 02 F-06108 Nice CEDEX, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Omid","family":"Feizbakhsh","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, CNRS, USR 3151, Protein Phosphorylation &amp; Human Diseases, Station Biologique de Roscoff, CS 90074, 29688 Roscoff CEDEX, France"},{"name":"Sorbonne Universit\u00e9, CNRS, UMR 8227, Integrative Biology of Marine Models, Station Biologique de Roscoff, CS 90074, 29688 Roscoff CEDEX, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B\u00e9atrice","family":"Foll-Josselin","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, CNRS, USR 3151, Protein Phosphorylation &amp; Human Diseases, Station Biologique de Roscoff, CS 90074, 29688 Roscoff CEDEX, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2064-9558","authenticated-orcid":false,"given":"Blandine","family":"Baratte","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, CNRS, USR 3151, Protein Phosphorylation &amp; Human Diseases, Station Biologique de Roscoff, CS 90074, 29688 Roscoff CEDEX, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claire","family":"Delehouz\u00e9","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, CNRS, USR 3151, Protein Phosphorylation &amp; Human Diseases, Station Biologique de Roscoff, CS 90074, 29688 Roscoff CEDEX, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arnaud","family":"Cousseau","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, CNRS, USR 3151, Protein Phosphorylation &amp; Human Diseases, Station Biologique de Roscoff, CS 90074, 29688 Roscoff CEDEX, France"},{"name":"UMR 7272 CNRS, Marine Natural Products Team, Nice Institute of Chemistry (ICN), University Nice Sophia Antipolis, Parc Valrose, 02 F-06108 Nice CEDEX, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xavier","family":"Fant","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, CNRS, USR 3151, Protein Phosphorylation &amp; Human Diseases, Station Biologique de Roscoff, CS 90074, 29688 Roscoff CEDEX, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeannette Chlo\u00eb","family":"Bulinski","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, CNRS, USR 3151, Protein Phosphorylation &amp; Human Diseases, Station Biologique de Roscoff, CS 90074, 29688 Roscoff CEDEX, France"},{"name":"Department of Biological Sciences, Columbia University, New York, NY 10027, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claude Elisabeth","family":"Payri","sequence":"additional","affiliation":[{"name":"UMR ENTROPIE (IRD\u2014Universit\u00e9 de La R\u00e9union\u2014CNRS), Laboratoire d\u2019Excellence Labex-CORAIL, Institut de Recherche pour le D\u00e9veloppement (IRD), BP A5, 98848 Noum\u00e9a CEDEX, New Caledonia, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4606-6625","authenticated-orcid":false,"given":"Sandrine","family":"Ruchaud","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, CNRS, USR 3151, Protein Phosphorylation &amp; 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