{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T11:10:03Z","timestamp":1790766603385,"version":"4.1.0"},"reference-count":35,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2019,3,25]],"date-time":"2019-03-25T00:00:00Z","timestamp":1553472000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Education, Culture, Sports, Science and Technology, Japan","award":["Regional Innovation Cluster Program (Global Type)"],"award-info":[{"award-number":["Regional Innovation Cluster Program (Global Type)"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>Plastid proteins are one of the main components in red algae. In order to clarify the angiotensin I converting enzyme (ACE) inhibitory peptides from red alga Palmaria sp. (Japan), we determined the plastid genome sequence. The genome possesses 205 protein coding genes, which were classified as genetic systems, ribosomal proteins, photosystems, adenosine triphosphate (ATP) synthesis, metabolism, transport, or unknown. After comparing ACE inhibitory peptides between protein sequences and a database, photosystems (177 ACE inhibitory peptides) were found to be the major source of ACE inhibitory peptides (total of 751). Photosystems consist of phycobilisomes, photosystem I, photosystem II, cytochrome complex, and a redox system. Among them, photosystem I (53) and II (51) were the major source of ACE inhibitory peptides. We found that the amino acid sequence of apcE (14) in phycobilisomes, psaA (18) and psaB (13) in photosystem I, and psbB (11) and psbC (10) in photosystem II covered a majority of bioactive peptide sequences. These results are useful for evaluating the bioactive peptides from red algae.<\/jats:p>","DOI":"10.3390\/md17030190","type":"journal-article","created":{"date-parts":[[2019,3,27]],"date-time":"2019-03-27T05:03:12Z","timestamp":1553662992000},"page":"190","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["In Silico Analysis of Relationship between Proteins from Plastid Genome of Red Alga Palmaria sp. (Japan) and Angiotensin I Converting Enzyme Inhibitory Peptides"],"prefix":"10.3390","volume":"17","author":[{"given":"Yuya","family":"Kumagai","sequence":"first","affiliation":[{"name":"Laboratory of Marine Chemical Resource Development, Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshikatsu","family":"Miyabe","sequence":"additional","affiliation":[{"name":"Chair of Marine Chemical Resource Development, Graduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoyuki","family":"Takeda","sequence":"additional","affiliation":[{"name":"Chair of Marine Chemical Resource Development, Graduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kohsuke","family":"Adachi","sequence":"additional","affiliation":[{"name":"Laboratory of Aquatic Product Utilization, Graduate School of Agriculture, Kochi University, Monobeotsu 200, Nankoku, Kochi 783-8502, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hajime","family":"Yasui","sequence":"additional","affiliation":[{"name":"Laboratory of Humans and the Ocean, Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1139-4047","authenticated-orcid":false,"given":"Hideki","family":"Kishimura","sequence":"additional","affiliation":[{"name":"Laboratory of Marine Chemical Resource Development, Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,3,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1007\/s10811-010-9632-5","article-title":"Bioactive compounds in seaweed: Functional food applications and legislation","volume":"23","author":"Holdt","year":"2011","journal-title":"J. Appl. Phycol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1002\/elsc.201400191","article-title":"Algae as production systems of bioactive compounds","volume":"15","author":"Michalak","year":"2015","journal-title":"Eng. Life Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/S0167-7799(96)10069-X","article-title":"Biotechnology and the red seaweed polysaccharide industry: Status, needs and prospects","volume":"15","author":"Renn","year":"1997","journal-title":"Trends Biotechnol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.carbpol.2011.12.029","article-title":"Biological activities and potential industrial applications of fucose rich sulfated polysaccharides and fucoidans isolated from brown seaweeds: A review","volume":"88","author":"Wijesinghe","year":"2012","journal-title":"Carbohydr. Polym."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1007\/s10811-015-0587-4","article-title":"Heat treatment increases the protein bioaccessibility in the red seaweed dulse (Palmaria Palmata), but not in the brown seaweed winged kelp (Alaria Esculenta)","volume":"28","author":"Maehre","year":"2016","journal-title":"J. Appl. Phycol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/0044-8486(94)90107-4","article-title":"Comparative studies on the nutrition of two species of abalone, Haliotis tuberculata L. and Haliotis discus hannai Ino: II. Amino acid composition of abalone and six species of macroalgae with an assessment of their nutritional value","volume":"128","author":"Mai","year":"1994","journal-title":"Aquaculture"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1111\/raq.12224","article-title":"Recent Developments in aquaculture of Palmaria palmata (Linnaeus) (Weber & Mohr 1805): Cultivation and uses","volume":"11","author":"Grote","year":"2019","journal-title":"Rev. Aquac."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1146\/annurev.pp.32.060181.001551","article-title":"Phycobilisomes","volume":"32","author":"Gantt","year":"1981","journal-title":"Annu. Rev. Plant Physiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"e12709","DOI":"10.1111\/jfbc.12709","article-title":"Antioxidant activity of proteins extracted from red alga dulse harvested in Japan","volume":"43","author":"Sato","year":"2018","journal-title":"J. Food Biochem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1757","DOI":"10.1016\/j.procbio.2014.06.022","article-title":"Concurrent purification and antioxidant activity of phycobiliproteins from Lyngbya Sp. A09DM: An antioxidant and anti-aging potential of phycoerythrin in Caenorhabditis Elegans","volume":"49","author":"Sonani","year":"2014","journal-title":"Process Biochem."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"3101","DOI":"10.1007\/s10811-016-0850-3","article-title":"Evaluation of the in vitro biological activity of protein hydrolysates of the edible red alga, Palmaria palmata (dulse) harvested from the Gaspe coast and cultivated in tanks","volume":"28","author":"Beaulieu","year":"2016","journal-title":"J. Appl. Phycol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1829","DOI":"10.1007\/s00217-017-2886-2","article-title":"Purification and identification of a novel ACE inhibitory peptide from marine alga Gracilariopsis lemaneiformis protein hydrolysate","volume":"243","author":"Cao","year":"2017","journal-title":"Eur. Food Res. Technol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1016\/S0955-2863(00)00110-8","article-title":"Identification of an antihypertensive peptide from peptic digest of wakame (Undaria pinnatifida)","volume":"11","author":"Suetsuna","year":"2000","journal-title":"J. Nutr. Biochem."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"7421","DOI":"10.1021\/jf301361c","article-title":"Isolation and characterization of bioactive pro-peptides with in vitro renin inhibitory activities from the macroalga Palmaria palmata","volume":"60","author":"Fitzgerald","year":"2012","journal-title":"J. Agric. Food Chem."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Furuta, T., Miyabe, Y., Yasui, H., Kinoshita, Y., and Kishimura, H. (2016). Angiotensin I converting enzyme inhibitory peptides derived from phycobiliproteins of dulse Palmaria palmata. Mar. Drugs, 14.","DOI":"10.3390\/md14020032"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1793","DOI":"10.1007\/s10811-013-0017-4","article-title":"In vitro assessment of the cardioprotective, anti-diabetic and antioxidant potential of Palmaria palmata protein hydrolysates","volume":"25","author":"Harnedy","year":"2013","journal-title":"J. Appl. Phycol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"746","DOI":"10.1016\/j.foodres.2014.04.035","article-title":"The effect of time and origin of harvest on the in vitro biological activity of Palmaria palmata protein hydrolysates","volume":"62","author":"Harnedy","year":"2014","journal-title":"Food Res. Int."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"3499","DOI":"10.1016\/j.biortech.2006.11.036","article-title":"High throughput and rapid screening of marine protein hydrolysates enriched in peptides with angiotensin-I-converting enzyme inhibitory activity by capillary electrophoresis","volume":"98","author":"He","year":"2007","journal-title":"Bioresour. Technol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/jfbc.12436","article-title":"Structural characteristics of phycobiliproteins from red alga Mazzaella japonica","volume":"42","author":"Kitade","year":"2018","journal-title":"J. Food Biochem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"e12301","DOI":"10.1111\/jfbc.12301","article-title":"Structural properties of phycoerythrin from dulse Palmaria palmata","volume":"41","author":"Miyabe","year":"2017","journal-title":"J. Food Biochem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1197","DOI":"10.1007\/s10811-012-9913-2","article-title":"Enzyme proteolysis enhanced extraction of ACE inhibitory and antioxidant compounds (peptides and polyphenols) from Porphyra columbina residual cake","volume":"25","author":"Cian","year":"2013","journal-title":"J. Appl. Phycol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"343","DOI":"10.4490\/ALGAE.2006.21.3.343","article-title":"Screening of extracts from red algae in Jeju for potentials marine angiotensin-I converting enzyme (ACE) inhibitory activity","volume":"21","author":"Cha","year":"2006","journal-title":"Algae"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"945","DOI":"10.1016\/j.idairyj.2005.10.012","article-title":"Bioactive peptides: Production and functionality","volume":"16","author":"Korhonen","year":"2006","journal-title":"Int. Dairy J."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"6245","DOI":"10.1021\/jf020482t","article-title":"Angiotensin I-converting enzyme inhibitory peptides derived from wakame (Undaria pinnatifida) and their antihypertensive effect in spontaneously hypertensive rats","volume":"50","author":"Sato","year":"2002","journal-title":"J. Agric. Food Chem."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Cho, C.H., Choi, J.W., Lam, D.W., Kim, K.M., and Yoon, H.S. (2018). Plastid genome analysis of three nemaliophycidae red algal species suggests environmental adaptation for iron limited habitats. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0196995"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Janouskovec, J., Liu, S.L., Martone, P.T., Carre, W., Leblanc, C., Collen, J., and Keeling, P.J. (2013). Evolution of red algal plastid genomes: Ancient architectures, introns, horizontal gene transfer, and taxonomic utility of plastid markers. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0059001"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1111\/jpy.12283","article-title":"The Ghost Plastid of Choreocolax Polysiphoniae","volume":"51","author":"Salomaki","year":"2015","journal-title":"J. Phycol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12915-016-0299-5","article-title":"Parallel evolution of highly conserved plastid genome architecture in red seaweeds and seed plants","volume":"14","author":"Lee","year":"2016","journal-title":"BMC Biol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"23744","DOI":"10.1038\/srep23744","article-title":"Reconstructing the complex evolutionary history of mobile plasmids in red algal genomes","volume":"6","author":"Lee","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"281","DOI":"10.6090\/jarq.35.281","article-title":"Production and use of marine algae in Japan","volume":"35","author":"Murata","year":"2001","journal-title":"Jpn. Agric. Res. Q."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1016\/S0955-2863(99)00014-5","article-title":"Nutritional value of proteins from edible seaweed Palmaria palmata (dulse)","volume":"10","author":"Fleurence","year":"1999","journal-title":"J. Nutr. Biochem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2523","DOI":"10.1007\/s10811-018-1440-3","article-title":"Phycoerythrin productivity and diversity from five red macroalgae","volume":"30","author":"Sfriso","year":"2018","journal-title":"J. Appl. Phycol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1007\/BF00003796","article-title":"Use of enzymatic cell wall degradation for improvement of protein extraction from Chondrus crispus, Gracilaria verrucosa and Palmaria palmata","volume":"7","author":"Fleurence","year":"1995","journal-title":"J. Appl. Phycol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1007\/BF02914055","article-title":"Ready-to-use DNA extracted with a CTAB method adapted for herbarium specimens and mucilaginous plant tissue","volume":"24","author":"Remarchuk","year":"2006","journal-title":"Plant Mol. Biol. Rep."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1093\/nar\/gkt289","article-title":"OrganellarGenomeDRAW\u2014A suite of tools for generating physical maps of plastid and mitochondrial genomes and visualizing expression data sets","volume":"41","author":"Lohse","year":"2013","journal-title":"Nucleic Acids Res."}],"container-title":["Marine Drugs"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1660-3397\/17\/3\/190\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T12:40:27Z","timestamp":1760186427000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1660-3397\/17\/3\/190"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,3,25]]},"references-count":35,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2019,3]]}},"alternative-id":["md17030190"],"URL":"https:\/\/doi.org\/10.3390\/md17030190","relation":{},"ISSN":["1660-3397"],"issn-type":[{"value":"1660-3397","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,3,25]]}}}