{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T06:32:10Z","timestamp":1792045930849,"version":"4.4.0"},"reference-count":66,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2019,3,29]],"date-time":"2019-03-29T00:00:00Z","timestamp":1553817600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>Macroalgae represent an important source of bioactive compounds with a wide range of biotechnological applications. Overall, the discovery of effective cytotoxic compounds with pharmaceutical potential is a significant challenge, mostly because they are scarce in nature or their total synthesis is not efficient, while the bioprospecting models currently used do not predict clinical responses. Given this context, we used three-dimensional (3D) cultures of human breast cancer explants to evaluate the antitumoral effect of laurinterol, the major compound of an ethanolic extract of Laurencia johnstonii. To this end, we evaluated the metabolic and histopathological effects of the crude extract of L. johnstonii and laurinterol on Vero and MCF-7 cells, in addition to breast cancer explants. We observed a dose-dependent inhibition of the metabolic activity, as well as morphologic and nuclear changes characteristic of apoptosis. On the other hand, a reduced metabolic viability and marked necrosis areas were observed in breast cancer explants incubated with the crude extract, while explants treated with laurinterol exhibited a heterogeneous response which was associated with the individual response of each human tumor sample. This study supports the cytotoxic and antitumoral effects of laurinterol in in vitro cell cultures and in ex vivo organotypic cultures of human breast cancer explants.<\/jats:p>","DOI":"10.3390\/md17040201","type":"journal-article","created":{"date-parts":[[2019,3,29]],"date-time":"2019-03-29T13:09:58Z","timestamp":1553864998000},"page":"201","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Antitumoral Effect of Laurinterol on 3D Culture of Breast Cancer Explants"],"prefix":"10.3390","volume":"17","author":[{"given":"Sara","family":"Garc\u00eda-Davis","sequence":"first","affiliation":[{"name":"Facultad de Ciencias Biol\u00f3gicas (FCB), Universidad Aut\u00f3noma de Nuevo Le\u00f3n (UANL), Av. Pedro de Alba s\/n, 66450 San Nicol\u00e1s de los Garza, Nuevo Le\u00f3n, M\u00e9xico"},{"name":"Instituto Universitario de Bio-Org\u00e1nica Antonio Gonz\u00e1lez (IUBO AG), Centro de Investigaciones Biom\u00e9dicas de Canarias (CIBICAN), Universidad de La Laguna (ULL), Avda. Astrof\u00edsico F. S\u00e1nchez, 2, 38206 La Laguna, Tenerife, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5944-7972","authenticated-orcid":false,"given":"Ezequiel","family":"Viveros-Valdez","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Biol\u00f3gicas (FCB), Universidad Aut\u00f3noma de Nuevo Le\u00f3n (UANL), Av. Pedro de Alba s\/n, 66450 San Nicol\u00e1s de los Garza, Nuevo Le\u00f3n, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8886-7519","authenticated-orcid":false,"given":"Ana R.","family":"D\u00edaz-Marrero","sequence":"additional","affiliation":[{"name":"Instituto Universitario de Bio-Org\u00e1nica Antonio Gonz\u00e1lez (IUBO AG), Centro de Investigaciones Biom\u00e9dicas de Canarias (CIBICAN), Universidad de La Laguna (ULL), Avda. Astrof\u00edsico F. S\u00e1nchez, 2, 38206 La Laguna, Tenerife, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0805-8317","authenticated-orcid":false,"given":"Jos\u00e9 J.","family":"Fern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Instituto Universitario de Bio-Org\u00e1nica Antonio Gonz\u00e1lez (IUBO AG), Centro de Investigaciones Biom\u00e9dicas de Canarias (CIBICAN), Universidad de La Laguna (ULL), Avda. Astrof\u00edsico F. 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G\u00f3mez s\/n, Colonia Centro, 64000 Monterrey, Nuevo Le\u00f3n, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8944-9298","authenticated-orcid":false,"given":"Pilar","family":"Carranza-Rosales","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica del Noreste (CIBIN), Instituto Mexicano del Seguro Social (IMSS), Calle Jes\u00fas Dionisio Gonz\u00e1lez # 501, Col. Independencia, 64720 Monterrey, Nuevo Le\u00f3n, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8800-1443","authenticated-orcid":false,"given":"Irma Edith","family":"Carranza-Torres","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Biol\u00f3gicas (FCB), Universidad Aut\u00f3noma de Nuevo Le\u00f3n (UANL), Av. Pedro de Alba s\/n, 66450 San Nicol\u00e1s de los Garza, Nuevo Le\u00f3n, M\u00e9xico"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica del Noreste (CIBIN), Instituto Mexicano del Seguro Social (IMSS), Calle Jes\u00fas Dionisio Gonz\u00e1lez # 501, Col. Independencia, 64720 Monterrey, Nuevo Le\u00f3n, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2925-0532","authenticated-orcid":false,"given":"Nancy Elena","family":"Guzm\u00e1n-Delgado","sequence":"additional","affiliation":[{"name":"Divisi\u00f3n de Investigaci\u00f3n en Salud, Unidad M\u00e9dica de Alta Especialidad, Hospital de Cardiolog\u00eda No. 34, Instituto Mexicano del Seguro Social (IMSS), Av. Lincoln S\/N esquina con Enfermera Mar\u00eda de Jes\u00fas Candia, Col. Valle Verde 2do. Sector, 64360 Monterrey, Nuevo Le\u00f3n, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,3,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"777","DOI":"10.3389\/fphar.2018.00777","article-title":"From marine origin to therapeutics: The antitumor potential of marine algae-derived compounds","volume":"9","author":"Alves","year":"2018","journal-title":"Front. Pharmacol."},{"key":"ref_2","unstructured":"Guiry, M.D., and Guiry, M.D. (2018, May 12). AlgaBase. World-wide electronic publication, National University of Irland, Galway. Available online: www.algaebase.org."},{"key":"ref_3","unstructured":"V\u00e1zquez-Borja, R. (1999). 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