{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T23:29:14Z","timestamp":1787527754586,"version":"build-2736575974"},"reference-count":23,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2019,4,15]],"date-time":"2019-04-15T00:00:00Z","timestamp":1555286400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the National Science Foundation from Guangdong Ocean University and  the National Science Foundation from Zhanjiang Technology Bureau","award":["Q15094\uff0cQ17092\uff0cQ14176, A15445\uff0cA14457\uff0c2016A02023, 2017C8B1"],"award-info":[{"award-number":["Q15094\uff0cQ17092\uff0cQ14176, A15445\uff0cA14457\uff0c2016A02023, 2017C8B1"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>In the study, the protective effect of plasma protein from Tachypleus tridentatus (PPTT) on acute kidney injury (AKI) and the related molecular mechanisms were first investigated by Western blotting analyses, TdT-mediated dUTP Nick-End Labeling (TUNEL) assay, and immunohistochemistry. It was found that PPTT had an obviously inhibitory effect on Reactive oxygen species (ROS) in cyclophosphamide (CTX)-exposed mice. Furthermore, results demonstrated that the renal cell death mode is due to inducing apoptosis and autophagy inhibited by dose-dependent PPTT in mice treated with CTX by decreasing the protein expression of bax, beclin-1, and LC3 and increasing the expression of bcl-2. Moreover, the p38 MAPK and PI3K\/Akt signaling pathways were observed to take part in the PPTT-induced renal cell growth effect by enhancing the upregulation of the expression of Akt and p-Akt as well as the downregulation of the expression of p38 and p-p38, which indicated a PPTT ameliorating effect on AKI CTX-induced in mice through p38 MAPK and PI3K\/Akt signaling pathways. Briefly, this article preliminarily studies the mechanism of the PPTT ameliorating effect on AKI CTX-induced in mice, which helps to provide a reference for PPTT clinical application in AKI therapy.<\/jats:p>","DOI":"10.3390\/md17040227","type":"journal-article","created":{"date-parts":[[2019,4,15]],"date-time":"2019-04-15T11:15:58Z","timestamp":1555326958000},"page":"227","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["The Ameliorating Effect of Plasma Protein from Tachypleus tridentatus on Cyclophosphamide-Induced Acute Kidney Injury in Mice"],"prefix":"10.3390","volume":"17","author":[{"given":"Xinhuang","family":"Kang","sequence":"first","affiliation":[{"name":"Department of Applied Chemistry, College of Chemistry and Environment, Guangdong Ocean University, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengyao","family":"Jing","sequence":"additional","affiliation":[{"name":"Department of Applied Chemistry, College of Chemistry and Environment, Guangdong Ocean University, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoguang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Applied Chemistry, College of Chemistry and Environment, Guangdong Ocean University, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lanzheng","family":"He","sequence":"additional","affiliation":[{"name":"Department of Applied Chemistry, College of Chemistry and Environment, Guangdong Ocean University, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengzhi","family":"Hong","sequence":"additional","affiliation":[{"name":"Department of Food Quality and Safety, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4461-1471","authenticated-orcid":false,"given":"Chunmei","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Applied Chemistry, College of Chemistry and Environment, Guangdong Ocean University, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,4,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1681\/ASN.2012080800","article-title":"Temporal changes in incidence of dialysis-requiring AKI","volume":"24","author":"Hsu","year":"2013","journal-title":"J. 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