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Enzyme-aided extraction of fucoidan by AMG (EAEF-AMG) significantly increased EPC proliferation by reducing the reactive oxygen species (ROS) and decreasing apoptosis. Notably, EAEF-AMG treated EPCs repressed the colocalization of TSC2\/LAMP1 and promoted perinuclear localization of mTOR\/LAMP1 and mTOR\/Rheb. Moreover, EAEF-AMG enhanced EPC functionalities, including tube formation, cell migration, and wound healing via regulation of AKT\/Rheb signaling. Our data provided cell priming protocols to enhance therapeutic applications of EPCs using bioactive compounds for the treatment of CVD.<\/jats:p>","DOI":"10.3390\/md17070392","type":"journal-article","created":{"date-parts":[[2019,7,3]],"date-time":"2019-07-03T11:14:49Z","timestamp":1562152489000},"page":"392","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Enzyme-Aided Extraction of Fucoidan by AMG Augments the Functionality of EPCs through Regulation of the AKT\/Rheb Signaling Pathway"],"prefix":"10.3390","volume":"17","author":[{"given":"Vinoth Kumar","family":"Rethineswaran","sequence":"first","affiliation":[{"name":"Convergence Stem Cell Research Center, Pusan National University, Yangsan 50612, Korea"},{"name":"Laboratory for Vascular Medicine and Stem Cell Biology, Department of Physiology, School of Medicine, Pusan National University, Yangsan 50612, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yeon-Ju","family":"Kim","sequence":"additional","affiliation":[{"name":"Convergence Stem Cell Research Center, Pusan National University, Yangsan 50612, Korea"},{"name":"Laboratory for Vascular Medicine and Stem Cell Biology, Department of Physiology, School of Medicine, Pusan National University, Yangsan 50612, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Woong Bi","family":"Jang","sequence":"additional","affiliation":[{"name":"Convergence Stem Cell Research Center, Pusan National University, Yangsan 50612, Korea"},{"name":"Laboratory for Vascular Medicine and Stem Cell Biology, Department of Physiology, School of Medicine, Pusan National University, Yangsan 50612, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seung Taek","family":"Ji","sequence":"additional","affiliation":[{"name":"Convergence Stem Cell Research Center, Pusan National University, Yangsan 50612, 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