{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T09:20:53Z","timestamp":1787908853020,"version":"build-2784847793"},"reference-count":25,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2019,7,19]],"date-time":"2019-07-19T00:00:00Z","timestamp":1563494400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>Acanthamoeba genus is a widely distributed and opportunistic parasite with increasing importance worldwide as an emerging pathogen in the past decades. This protozoan has an active trophozoite stage, a cyst stage, and is dormant and very resistant. It can cause Acanthamoeba keratitis, an ocular sight-threatening disease, and granulomatous amoebic encephalitis, a chronic, very fatal brain pathology. In this study, the amoebicidal activity of sixteen Laurencia oxasqualenoid metabolites and semisynthetic derivatives were tested against Acanthamoeba castellanii Neff. The results obtained point out that iubol (3) and dehydrothyrsiferol (1) possess potent activities, with IC50 values of 5.30 and 12.83 \u00b5M, respectively. The hydroxylated congeners thyrsiferol (2) and 22-hydroxydehydrothyrsiferol (4), active in the same value range at IC50 13.97 and 17.00 \u00b5M, are not toxic against murine macrophages; thus, they are solid candidates for the development of new amoebicidal therapies.<\/jats:p>","DOI":"10.3390\/md17070420","type":"journal-article","created":{"date-parts":[[2019,7,22]],"date-time":"2019-07-22T02:55:37Z","timestamp":1563764137000},"page":"420","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Evaluation of Oxasqualenoids from the Red Alga Laurencia viridis against Acanthamoeba"],"prefix":"10.3390","volume":"17","author":[{"given":"Jacob","family":"Lorenzo-Morales","sequence":"first","affiliation":[{"name":"Instituto Universitario de Enfermedades Tropicales y Salud P\u00fablica de Islas Canarias, Universidad de La Laguna, Avda. Astrof\u00edsico F. S\u00e1nchez s\/n, 38206 La Laguna, Tenerife, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana R.","family":"D\u00edaz-Marrero","sequence":"additional","affiliation":[{"name":"Instituto Universitario de Bio-Org\u00e1nica Antonio Gonz\u00e1lez (IUBO AG), Centro de Investigaciones Biom\u00e9dicas de Canarias (CIBICAN), Universidad de La Laguna (ULL), Avda. Astrof\u00edsico F. S\u00e1nchez, 2, 38206 La Laguna, Tenerife, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8506-2435","authenticated-orcid":false,"given":"Francisco","family":"Cen-Pacheco","sequence":"additional","affiliation":[{"name":"Instituto Universitario de Bio-Org\u00e1nica Antonio Gonz\u00e1lez (IUBO AG), Centro de Investigaciones Biom\u00e9dicas de Canarias (CIBICAN), Universidad de La Laguna (ULL), Avda. Astrof\u00edsico F. S\u00e1nchez, 2, 38206 La Laguna, Tenerife, Spain"},{"name":"Facultad de Bioan\u00e1lisis, Campus-Veracruz, Universidad Veracruzana, Veracruz 91700, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ines","family":"Sifaoui","sequence":"additional","affiliation":[{"name":"Instituto Universitario de Enfermedades Tropicales y Salud P\u00fablica de Islas Canarias, Universidad de La Laguna, Avda. Astrof\u00edsico F. S\u00e1nchez s\/n, 38206 La Laguna, Tenerife, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda","family":"Reyes-Batlle","sequence":"additional","affiliation":[{"name":"Instituto Universitario de Enfermedades Tropicales y Salud P\u00fablica de Islas Canarias, Universidad de La Laguna, Avda. Astrof\u00edsico F. S\u00e1nchez s\/n, 38206 La Laguna, Tenerife, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda L.","family":"Souto","sequence":"additional","affiliation":[{"name":"Instituto Universitario de Bio-Org\u00e1nica Antonio Gonz\u00e1lez (IUBO AG), Centro de Investigaciones Biom\u00e9dicas de Canarias (CIBICAN), Universidad de La Laguna (ULL), Avda. Astrof\u00edsico F. S\u00e1nchez, 2, 38206 La Laguna, Tenerife, Spain"},{"name":"Departamento de Qu\u00edmica Org\u00e1nica, Universidad de La Laguna (ULL), Avda. Astrof\u00edsico F. S\u00e1nchez, 2, 38206 La Laguna, Tenerife, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Hern\u00e1ndez Daranas","sequence":"additional","affiliation":[{"name":"Instituto Universitario de Bio-Org\u00e1nica Antonio Gonz\u00e1lez (IUBO AG), Centro de Investigaciones Biom\u00e9dicas de Canarias (CIBICAN), Universidad de La Laguna (ULL), Avda. Astrof\u00edsico F. S\u00e1nchez, 2, 38206 La Laguna, Tenerife, Spain"},{"name":"Instituto de Productos Naturales y Agrobiolog\u00eda (IPNA), Consejo Superior de Investigaciones Cient\u00edficas (CSIC), Avenida Astrof\u00edsico Francisco S\u00e1nchez 2, 38206 Tenerife, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6233-8224","authenticated-orcid":false,"given":"Jos\u00e9 E.","family":"Pi\u00f1ero","sequence":"additional","affiliation":[{"name":"Instituto Universitario de Enfermedades Tropicales y Salud P\u00fablica de Islas Canarias, Universidad de La Laguna, Avda. Astrof\u00edsico F. S\u00e1nchez s\/n, 38206 La Laguna, Tenerife, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0805-8317","authenticated-orcid":false,"given":"Jos\u00e9 J.","family":"Fern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Instituto Universitario de Bio-Org\u00e1nica Antonio Gonz\u00e1lez (IUBO AG), Centro de Investigaciones Biom\u00e9dicas de Canarias (CIBICAN), Universidad de La Laguna (ULL), Avda. Astrof\u00edsico F. S\u00e1nchez, 2, 38206 La Laguna, Tenerife, Spain"},{"name":"Departamento de Qu\u00edmica Org\u00e1nica, Universidad de La Laguna (ULL), Avda. Astrof\u00edsico F. S\u00e1nchez, 2, 38206 La Laguna, Tenerife, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,7,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1128\/CMR.16.2.273-307.2003","article-title":"Acanthamoeba spp. as Agents of Disease in Humans","volume":"16","author":"Cabral","year":"2003","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1111\/j.1574-6976.2006.00023.x","article-title":"Acanthamoeba: Biology and increasing importance in human health","volume":"30","author":"Khan","year":"2006","journal-title":"FEMS Microbiol. 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