{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T09:38:22Z","timestamp":1790069902420,"version":"4.0.1"},"reference-count":110,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2019,7,25]],"date-time":"2019-07-25T00:00:00Z","timestamp":1564012800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"DGAPA-PAPIIT-UNAM, MEXICO","award":["IT-200819"],"award-info":[{"award-number":["IT-200819"]}]},{"name":"CONACYT, M\u00c9XICO","award":["178151"],"award-info":[{"award-number":["178151"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>Sea anemone venom contains a complex and diverse arsenal of peptides and proteins of pharmacological and biotechnological interest, however, only venom from a few species has been explored from a global perspective to date. In the present study, we identified the polypeptides present in the venom of the sea anemone Anthopleura dowii Verrill, 1869 through a transcriptomic and proteomic analysis of the tentacles and the proteomic profile of the secreted mucus. In our transcriptomic results, we identified 261 polypeptides related to or predicted to be secreted in the venom, including proteases, neurotoxins that could act as either potassium (K+) or sodium (Na+) channels inhibitors, protease inhibitors, phospholipases A2, and other polypeptides. Our proteomic data allowed the identification of 156 polypeptides\u201448 exclusively identified in the mucus, 20 in the tentacles, and 88 in both protein samples. Only 23 polypeptides identified by tandem mass spectrometry (MS\/MS) were related to the venom and 21 exclusively identified in the mucus, most corresponding to neurotoxins and hydrolases. Our data contribute to the knowledge of evolutionary and venomic analyses of cnidarians, particularly of sea anemones.<\/jats:p>","DOI":"10.3390\/md17080436","type":"journal-article","created":{"date-parts":[[2019,7,26]],"date-time":"2019-07-26T08:45:39Z","timestamp":1564130739000},"page":"436","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Transcriptomic and Proteomic Analysis of the Tentacles and Mucus of Anthopleura dowii Verrill, 1869"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8052-9793","authenticated-orcid":false,"given":"Santos","family":"Ram\u00edrez-Carreto","sequence":"first","affiliation":[{"name":"Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnolog\u00eda, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Avenida Universidad 2001, Cuernavaca, Morelos 62210, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosario","family":"Vera-Estrella","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Molecular de Plantas, Instituto de Biotecnolog\u00eda, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Avenida Universidad 2001, Cuernavaca, Morelos 62210, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3448-7959","authenticated-orcid":false,"given":"Tob\u00edas","family":"Portillo-Bobadilla","sequence":"additional","affiliation":[{"name":"Unidad de Bioinform\u00e1tica, Bioestad\u00edstica y Biolog\u00eda Computacional. Red de Apoyo a la Investigaci\u00f3n, Coordinaci\u00f3n de la Investigaci\u00f3n Cient\u00edfica, Universidad Nacional Aut\u00f3noma de M\u00e9xico-Instituto Nacional De Ciencias M\u00e9dicas y Nutrici\u00f3n Salvador Zubir\u00e1n, Calle Vasco de Quiroga 15, Tlalpan, C.P. 14080, Ciudad de M\u00e9xico, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4022-7405","authenticated-orcid":false,"given":"Alexei","family":"Licea-Navarro","sequence":"additional","affiliation":[{"name":"Departamento de Innovaci\u00f3n Biom\u00e9dica, CICESE, Carretera Ensenada-Tijuana 3918, Ensenada, BC C.P. 22860, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4894-5604","authenticated-orcid":false,"given":"Johanna","family":"Bernaldez-Sarabia","sequence":"additional","affiliation":[{"name":"Departamento de Innovaci\u00f3n Biom\u00e9dica, CICESE, Carretera Ensenada-Tijuana 3918, Ensenada, BC C.P. 22860, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8170-6379","authenticated-orcid":false,"given":"Enrique","family":"Rudi\u00f1o-Pi\u00f1era","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnolog\u00eda, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Avenida Universidad 2001, Cuernavaca, Morelos 62210, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7484-8739","authenticated-orcid":false,"given":"Jerome J.","family":"Verleyen","sequence":"additional","affiliation":[{"name":"Unidad Universitaria de Secuenciaci\u00f3n Masiva y Bioinform\u00e1tica, Instituto de Biotecnolog\u00eda, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Avenida Universidad 2001, Cuernavaca, Morelos 62210, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Estefan\u00eda","family":"Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Division of Invertebrate Zoology, American Museum of Natural History, Central Park West at 79th Street, New York, NY 10024, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8586-2317","authenticated-orcid":false,"given":"Claudia","family":"Rodr\u00edguez-Almaz\u00e1n","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnolog\u00eda, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Avenida Universidad 2001, Cuernavaca, Morelos 62210, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,7,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1812","DOI":"10.3390\/md10081812","article-title":"Sea Anemone (Cnidaria, Anthozoa, Actiniaria) Toxins: An Overview","volume":"10","author":"Vasconcelos","year":"2012","journal-title":"Mar. 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