{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T06:19:55Z","timestamp":1770358795421,"version":"3.49.0"},"reference-count":60,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2019,11,28]],"date-time":"2019-11-28T00:00:00Z","timestamp":1574899200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2015\/17177-6"],"award-info":[{"award-number":["2015\/17177-6"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>Rocas Atoll is a unique environment in the equatorial Atlantic Ocean, hosting a large number of endemic species, however, studies on the chemical diversity emerging from this biota are rather scarce. Therefore, the present work aims to assess the metabolomic diversity and pharmacological potential of the microbiota from Rocas Atoll. A total of 76 bacteria were isolated and cultured in liquid culture media to obtain crude extracts. About one third (34%) of these extracts were recognized as cytotoxic against human colon adenocarcinoma HCT-116 cell line. 16S rRNA gene sequencing analyses revealed that the bacteria producing cytotoxic extracts were mainly from the Actinobacteria phylum, including Streptomyces, Salinispora, Nocardiopsis, and Brevibacterium genera, and in a smaller proportion from Firmicutes phylum (Bacillus). The search in the spectral library in GNPS (Global Natural Products Social Molecular Networking) unveiled a high chemodiversity being produced by these bacteria, including rifamycins, antimycins, desferrioxamines, ferrioxamines, surfactins, surugamides, staurosporines, and saliniketals, along with several unidentified compounds. Using an original approach, molecular networking successfully highlighted groups of compounds responsible for the cytotoxicity of crude extracts. Application of DEREPLICATOR+ (GNPS) allowed the annotation of macrolide novonestimycin derivatives as the cytotoxic compounds existing in the extracts produced by Streptomyces BRB-298 and BRB-302. Overall, these results highlighted the pharmacological potential of bacteria from this singular atoll.<\/jats:p>","DOI":"10.3390\/md17120671","type":"journal-article","created":{"date-parts":[[2019,11,28]],"date-time":"2019-11-28T10:54:10Z","timestamp":1574938450000},"page":"671","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Marine Bacteria from Rocas Atoll as a Rich Source of Pharmacologically Active Compounds"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2697-8085","authenticated-orcid":false,"given":"Karen Y.","family":"Velasco-Alzate","sequence":"first","affiliation":[{"name":"Departamento de Farmacologia, Instituto de Ci\u00eancias Biom\u00e9dicas, Universidade de S\u00e3o Paulo, 05508-900 S\u00e3o Paulo\/SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9731-6172","authenticated-orcid":false,"given":"Anelize","family":"Bauermeister","sequence":"additional","affiliation":[{"name":"Departamento de Farmacologia, Instituto de Ci\u00eancias Biom\u00e9dicas, Universidade de S\u00e3o Paulo, 05508-900 S\u00e3o Paulo\/SP, Brazil"},{"name":"NPPNS, Departamento de F\u00edsica e Qu\u00edmica, Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto, Universidade de S\u00e3o Paulo, 14040-903 Ribeir\u00e3o Preto\/SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4236-094X","authenticated-orcid":false,"given":"Marcelo M. P.","family":"Tangerina","sequence":"additional","affiliation":[{"name":"Departamento do Bot\u00e2nica, Instituto de Bioci\u00eancias, Universidade de S\u00e3o Paulo, 05508-090 S\u00e3o Paulo\/SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3630-1342","authenticated-orcid":false,"given":"Tito M. C.","family":"Lotufo","sequence":"additional","affiliation":[{"name":"Departamento de Oceanografia Biol\u00f3gica, Instituto Oceanogr\u00e1fico, Universidade de S\u00e3o Paulo, 05508-120 S\u00e3o Paulo\/SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1877-1762","authenticated-orcid":false,"given":"Marcelo J. P.","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Departamento do Bot\u00e2nica, Instituto de Bioci\u00eancias, Universidade de S\u00e3o Paulo, 05508-090 S\u00e3o Paulo\/SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paula C.","family":"Jimenez","sequence":"additional","affiliation":[{"name":"Departamento de Ci\u00eancias do Mar, Universidade Federal de S\u00e3o Paulo, 11015-020 Santos\/SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriel","family":"Padilla","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia, Instituto de Ci\u00eancias Biom\u00e9dicas, Universidade de S\u00e3o Paulo, 05508-900 S\u00e3o Paulo\/SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8159-3658","authenticated-orcid":false,"given":"Norberto P.","family":"Lopes","sequence":"additional","affiliation":[{"name":"NPPNS, Departamento de F\u00edsica e Qu\u00edmica, Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto, Universidade de S\u00e3o Paulo, 14040-903 Ribeir\u00e3o Preto\/SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1861-5153","authenticated-orcid":false,"given":"Let\u00edcia V.","family":"Costa-Lotufo","sequence":"additional","affiliation":[{"name":"Departamento de Farmacologia, Instituto de Ci\u00eancias Biom\u00e9dicas, Universidade de S\u00e3o Paulo, 05508-900 S\u00e3o Paulo\/SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,11,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1039\/C7NP00052A","article-title":"Marine natural products","volume":"35","author":"Blunt","year":"2018","journal-title":"Nat. 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