{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T09:07:38Z","timestamp":1782810458697,"version":"3.54.5"},"reference-count":50,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2019,12,19]],"date-time":"2019-12-19T00:00:00Z","timestamp":1576713600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31972163"],"award-info":[{"award-number":["31972163"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"the Construction of Fishing Ports and the Development of Fishery Industry of Guangdong Province, china","award":["A201708C13"],"award-info":[{"award-number":["A201708C13"]}]},{"name":"Science and Technology Development Program of Shenzhen city, China","award":["JCYJ20170818111335796"],"award-info":[{"award-number":["JCYJ20170818111335796"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>Heparin from mollusks with unique sulfated glycosaminoglycan exhibits strong anti-thrombotic activities. This study reports on a purified heparinoid from Coelomactra antiquata, which shows potent anticoagulant and fibrinolytic abilities. Its structure was characterized by infrared spectroscopy, high-performance liquid chromatography, and one-dimensional and two-dimensional nuclear magnetic resonance spectroscopy. Its fibrinolytic activity was determined in vitro and in vivo. Its anticoagulant activity was determined by activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT). The results indicated that clam heparinoid was a homogeneous glycosaminoglycan with a molecular weight of 30.99 kDa, mainly composed of \u21924)-\u03b1-IdoA2S-(1\u21924)-\u03b1-GlcNS3S6S (or GlcNS6S)-(1\u21924)-\u03b2-GlcA-(1\u21924)-\u03b1-GlcNS6S (or GlcNAC)-(1\u2192. Furthermore, this heparinoid showed a highly anticoagulant titer and fibrinolytic value of 149.63 IU\/mg and 1.96 IU\/mg, respectively. In summary, clam heparinoid shows great potential for application in the clinic and antithrombotic drugs industry.<\/jats:p>","DOI":"10.3390\/md18010006","type":"journal-article","created":{"date-parts":[[2019,12,20]],"date-time":"2019-12-20T09:50:33Z","timestamp":1576835433000},"page":"6","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Isolation and Characterization of a Heparin-Like Compound with Potent Anticoagulant and Fibrinolytic Activity from the Clam Coelomactra antiquata"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6849-0147","authenticated-orcid":false,"given":"ZhenXing","family":"Du","sequence":"first","affiliation":[{"name":"School of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China"},{"name":"Shenzhen institute, Guangdong Ocean University, Shenzhen 518108, China"},{"name":"Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"XueJing","family":"Jia","sequence":"additional","affiliation":[{"name":"School of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China"},{"name":"Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China"},{"name":"Shenzhen institute, Guangdong Ocean University, Shenzhen 518108, China"},{"name":"Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"SiYi","family":"Zhou","sequence":"additional","affiliation":[{"name":"School of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China"},{"name":"Shenzhen institute, Guangdong Ocean University, Shenzhen 518108, China"},{"name":"Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"JianPing","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"XiaoFei","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5307-2708","authenticated-orcid":false,"given":"XiaoHuang","family":"Cao","sequence":"additional","affiliation":[{"name":"School of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"SaiYi","family":"Zhong","sequence":"additional","affiliation":[{"name":"School of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China"},{"name":"Shenzhen institute, Guangdong Ocean University, Shenzhen 518108, China"},{"name":"Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Zhanjiang 524088, China"},{"name":"Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian 116034, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"PengZhi","family":"Hong","sequence":"additional","affiliation":[{"name":"School of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China"},{"name":"Shenzhen institute, Guangdong Ocean University, Shenzhen 518108, China"},{"name":"Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,12,19]]},"reference":[{"key":"ref_1","first-page":"6","article-title":"Occurrence and structural characterization of heparin from molluscs","volume":"2","author":"Volpi","year":"2005","journal-title":"Invertebr. 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