{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T23:57:14Z","timestamp":1789430234380,"version":"build-2803163510"},"reference-count":52,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2020,11,20]],"date-time":"2020-11-20T00:00:00Z","timestamp":1605830400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","award":["MOST 109-2313-B-032-002-; MOST 109-2811-B-032-500"],"award-info":[{"award-number":["MOST 109-2313-B-032-002-; MOST 109-2811-B-032-500"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>Microbial fermentation of by-products is a renewable and efficient technique in the development of a range of useful products. In this study, protease synthesis by Paenibacillus sp. TKU052 was carried out on culture media containing some common seafood processing by-products (SPBPs) as the sole source of carbon and nitrogen (C\/N). The most suitable C\/N nutrition source for the production of proteases was found to be 3.0% (w\/v) demineralized crab shells powder (deCSP) and maximal enzyme activity of 4.41 \u00b1 0.16 U\/mL was detected on the third day of the culture. Two proteases (P1 and P2) with a similar molecular weight of 31 kDa were successfully isolated and purified from the 3-day deCSP-containing medium. Both P1 and P2 exhibited the highest activity of gelatin hydrolysis at pH 6 and 60 \u00b0C. The gelatin hydrolysates catalyzed by Paenibacillus TKU052 proteases were evaluated for biological activities, including 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, angiotensin-I converting enzyme (ACE) inhibition, and prebiotic activities. The gelatin hydrolysates expressed 31.76\u201343.95% DPPH radical scavenging activity and 31.58\u201336.84% ACE inhibitory activity, which was higher than those from gelatin. Gelatin hydrolysates also showed the growth-enhancing effect on Bifidobacterium bifidum BCRC 14615 with an increase to 135.70\u2013147.81%. In short, Paenibacillus sp. TKU052 could be a potential strain to utilize crab shell wastes to produce proteases for bio-active peptides\u2019 preparation.<\/jats:p>","DOI":"10.3390\/md18110574","type":"journal-article","created":{"date-parts":[[2020,11,20]],"date-time":"2020-11-20T09:46:18Z","timestamp":1605865578000},"page":"574","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Utilization of Seafood Processing By-Products for Production of Proteases by Paenibacillus sp. TKU052 and Their Application in Biopeptides\u2019 Preparation"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9379-0923","authenticated-orcid":false,"given":"Chien Thang","family":"Doan","sequence":"first","affiliation":[{"name":"Department of Natural Science and Technology, Tay Nguyen University, Buon Ma Thuot 630000, Vietnam"},{"name":"Department of Chemistry, Tamkang University, New Taipei City 25137, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thi Ngoc","family":"Tran","sequence":"additional","affiliation":[{"name":"Department of Natural Science and Technology, Tay Nguyen University, Buon Ma Thuot 630000, Vietnam"},{"name":"Department of Chemistry, Tamkang University, New Taipei City 25137, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2666-9584","authenticated-orcid":false,"given":"Van Bon","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology and Environment, Tay Nguyen University, Buon Ma Thuot 630000, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2335-1084","authenticated-orcid":false,"given":"Anh Dzung","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology and Environment, Tay Nguyen University, Buon Ma Thuot 630000, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1384-3731","authenticated-orcid":false,"given":"San-Lang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Tamkang University, New Taipei City 25137, Taiwan"},{"name":"Life Science Development Center, Tamkang University, New Taipei City 25137, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,11,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1007\/s10989-019-09823-5","article-title":"A review on bioactive peptides: Physiological functions, bioavailability and safety","volume":"26","author":"Bhandari","year":"2020","journal-title":"Int. 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