{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T03:34:48Z","timestamp":1787974488557,"version":"build-2784847793"},"reference-count":39,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2021,1,10]],"date-time":"2021-01-10T00:00:00Z","timestamp":1610236800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>Microalgae are at the start of the food chain, and many are known producers of a significant amount of lipids with essential fatty acids. However, the bioactivity of microalgal lipids for anti-inflammatory and antithrombotic activities have rarely been investigated. Therefore, for a sustainable source of the above bioactive lipids, the present study was undertaken. The total lipids of microalga Chlorococcum sp., isolated from the Irish coast, were fractionated into neutral-, glyco-, and phospho-lipids, and were tested in vitro for their anti-inflammatory and antithrombotic activities. All tested lipid fractions showed strong anti-platelet-activating factor (PAF) and antithrombin activities in human platelets (half maximal inhibitory concentration (IC50) values ranging ~25\u2013200 \u03bcg of lipid) with the highest activities in glyco- and phospho-lipid fractions. The structural analysis of the bioactive lipid fraction-2 revealed the presence of specific sulfoquinovosyl diacylglycerols (SQDG) bioactive molecules and the HexCer-t36:2 (t18:1\/18:1 and 18:2\/18:0) cerebrosides with a phytosphingosine (4-hydrosphinganine) base, while fraction-3 contained bioactive phosphatidylcholine (PC) and phosphatidylethanolamine (PE) molecules. These novel bioactive lipids of Chlorococcum sp. with putative health benefits may indicate that marine microalgae can be a sustainable alternative source for bioactive lipids production for food supplements and nutraceutical applications. However, further studies are required towards the commercial technology pathways development and biosafety analysis for the use of the microalga.<\/jats:p>","DOI":"10.3390\/md19010028","type":"journal-article","created":{"date-parts":[[2021,1,10]],"date-time":"2021-01-10T21:21:45Z","timestamp":1610313705000},"page":"28","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":48,"title":["Bioactive Lipids of Marine Microalga Chlorococcum sp. SABC 012504 with Anti-Inflammatory and Anti-Thrombotic Activities"],"prefix":"10.3390","volume":"19","author":[{"given":"Katie","family":"Shiels","sequence":"first","affiliation":[{"name":"Shannon Applied Biotechnology Centre, Limerick Institute of Technology, Moylish Park, V94 E8YF Limerick, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0837-9778","authenticated-orcid":false,"given":"Alexandros","family":"Tsoupras","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Limerick, V94 T9PX Limerick, Ireland"},{"name":"Health Research Institute, University of Limerick, V94 T9PX Limerick, Ireland"},{"name":"Bernal Institute, University of Limerick, V94 T9PX Limerick, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9668-3368","authenticated-orcid":false,"given":"Ronan","family":"Lordan","sequence":"additional","affiliation":[{"name":"Institute for Translational Medicine and Therapeutics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1854-9117","authenticated-orcid":false,"given":"Constantina","family":"Nasopoulou","sequence":"additional","affiliation":[{"name":"Department of Food Science and Nutrition, School of the Environment, University of the Aegean, GR 81400 Myrina, Lemnos, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8175-950X","authenticated-orcid":false,"given":"Ioannis","family":"Zabetakis","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Limerick, V94 T9PX Limerick, Ireland"},{"name":"Health Research Institute, University of Limerick, V94 T9PX Limerick, Ireland"},{"name":"Bernal Institute, University of Limerick, V94 T9PX Limerick, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6349-7761","authenticated-orcid":false,"given":"Patrick","family":"Murray","sequence":"additional","affiliation":[{"name":"Shannon Applied Biotechnology Centre, Limerick Institute of Technology, Moylish Park, V94 E8YF Limerick, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3981-8833","authenticated-orcid":false,"given":"Sushanta Kumar","family":"Saha","sequence":"additional","affiliation":[{"name":"Shannon Applied Biotechnology Centre, Limerick Institute of Technology, Moylish Park, V94 E8YF Limerick, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,1,10]]},"reference":[{"key":"ref_1","unstructured":"Becker, E.W. 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