{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T21:40:06Z","timestamp":1769722806902,"version":"3.49.0"},"reference-count":70,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2021,2,11]],"date-time":"2021-02-11T00:00:00Z","timestamp":1613001600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["APP1072113"],"award-info":[{"award-number":["APP1072113"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["APP1119056"],"award-info":[{"award-number":["APP1119056"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>The peripheral effects of \u03c9-conotoxins, selective blockers of N-type voltage-gated calcium channels (CaV2.2), have not been characterised across different clinically relevant pain models. This study examines the effects of locally administered \u03c9-conotoxin MVIIA, GVIA, and CVIF on mechanical and thermal paw withdrawal threshold (PWT) in postsurgical pain (PSP), cisplatin-induced neuropathy (CisIPN), and oxaliplatin-induced neuropathy (OIPN) rodent models. Intraplantar injection of 300, 100 and 30 nM MVIIA significantly (p &lt; 0.0001, p &lt; 0.0001, and p &lt; 0.05, respectively) alleviated mechanical allodynia of mice in PSP model compared to vehicle control group. Similarly, intraplantar injection of 300, 100, and 30 nM MVIIA (p &lt; 0.0001, p &lt; 0.01, and p &lt; 0.05, respectively), and 300 nM and 100 nM GVIA (p &lt; 0.0001 and p &lt; 0.05, respectively) significantly increased mechanical thresholds of mice in OIPN model. The ED50 of GVIA and MVIIA in OIPN was found to be 1.8 pmol\/paw and 0.8 pmol\/paw, respectively. However, none of the \u03c9-conotoxins were effective in a mouse model of CisIPN. The intraplantar administration of 300 nM GVIA, MVIIA, and CVIF did not cause any locomotor side effects. The intraplantar administration of MVIIA can alleviate incision-induced mechanical allodynia, and GVIA and MVIIA effectively reduce OIPN associated mechanical pain, without locomotor side effects, in rodent models. In contrast, CVIF was inactive in these pain models, suggesting it is unable to block a subset of N-type voltage-gated calcium channels associated with nociceptors in the skin.<\/jats:p>","DOI":"10.3390\/md19020106","type":"journal-article","created":{"date-parts":[[2021,2,12]],"date-time":"2021-02-12T18:45:00Z","timestamp":1613155500000},"page":"106","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Subcutaneous \u03c9-Conotoxins Alleviate Mechanical Pain in Rodent Models of Acute Peripheral Neuropathy"],"prefix":"10.3390","volume":"19","author":[{"given":"Md. Mahadhi","family":"Hasan","sequence":"first","affiliation":[{"name":"Division of Chemistry and Structural Biology, Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD 4072, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hana","family":"Starobova","sequence":"additional","affiliation":[{"name":"Division of Chemistry and Structural Biology, Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD 4072, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7447-1310","authenticated-orcid":false,"given":"Alexander","family":"Mueller","sequence":"additional","affiliation":[{"name":"Division of Chemistry and Structural Biology, Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD 4072, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3594-9588","authenticated-orcid":false,"given":"Irina","family":"Vetter","sequence":"additional","affiliation":[{"name":"Division of Chemistry and Structural Biology, Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD 4072, Australia"},{"name":"School of Pharmacy, The University of Queensland, Brisbane, QLD 4102, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3470-923X","authenticated-orcid":false,"given":"Richard J.","family":"Lewis","sequence":"additional","affiliation":[{"name":"Division of Chemistry and Structural Biology, Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD 4072, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,2,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1124\/pr.111.005322","article-title":"Conus venom peptide pharmacology","volume":"64","author":"Lewis","year":"2012","journal-title":"Pharmacol. Rev."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1159\/000438449","article-title":"Prey-capture strategies of fish-hunting cone snails: Behavior, neurobiology and evolution","volume":"86","author":"Olivera","year":"2015","journal-title":"Brain Behav. Evol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"193","DOI":"10.3390\/md403193","article-title":"\u03c9-Conotoxins GVIA, MVIIA and CVID: SAR and clinical potential","volume":"4","author":"Schroeder","year":"2006","journal-title":"Mar. Drugs"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1016\/0006-8993(94)90398-0","article-title":"Neuroanatomical distribution of receptors for a novel voltage-sensitive calcium-channel antagonist, SNX-230 (\u03c9-conopeptide MVIIC)","volume":"653","author":"Gohil","year":"1994","journal-title":"Brain Res."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1099","DOI":"10.1016\/0896-6273(92)90069-P","article-title":"Biochemical properties and subcellular distribution of an N-type calcium hannel and \u03b12\u03b4 subunit","volume":"9","author":"Westenbroek","year":"1992","journal-title":"Neuron"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1124\/pr.57.4.5","article-title":"Nomenclature and structure-function relationships of voltage-gated calcium channels","volume":"57","author":"Catterall","year":"2005","journal-title":"Pharmacol. Rev."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1038\/8070","article-title":"Splicing of \u03b11A subunit gene generates phenotypic variants of P- and Q-type calcium channels","volume":"2","author":"Bourinet","year":"1999","journal-title":"Nature Neurosci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2950","DOI":"10.1111\/j.1460-9568.2007.05912.x","article-title":"Novel CaV2.1 clone replicates many properties of Purkinje cell CaV2.1 current","volume":"26","author":"Richards","year":"2007","journal-title":"Eur. J. Neurosci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1152\/physrev.00018.2002","article-title":"Molecular physiology of low-voltage-activated T-type calcium channels","volume":"83","year":"2003","journal-title":"Physiol. Rev."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1016\/0006-8993(88)90790-1","article-title":"Autoradiographic visualization in rat brain of receptors for \u03c9-conotoxin GVIA, a newly discovered calcium antagonist","volume":"451","author":"Takemura","year":"1988","journal-title":"Brain Res."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1038\/308282a0","article-title":"A venom peptide with a novel presynaptic blocking action","volume":"308","author":"Kerr","year":"1984","journal-title":"Nature"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"3029","DOI":"10.2174\/0929867043363884","article-title":"Ziconotide: Neuronal calcium channel blocker for treating severe chronic pain","volume":"11","author":"Miljanich","year":"2004","journal-title":"Curr. Med. Chem."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1338","DOI":"10.1126\/science.4071055","article-title":"Peptide neurotoxins from fish-hunting cone snails","volume":"230","author":"Olivera","year":"1985","journal-title":"Science"},{"key":"ref_14","first-page":"274","article-title":"Ziconotide, a new N-type calcium channel blocker, administered intrathecally for acute postoperative pain","volume":"25","author":"Atanassoff","year":"2000","journal-title":"Reg. Anesth. Pain Med."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1325","DOI":"10.1038\/sj.bjp.0703701","article-title":"Cardiovascular and autonomic effects of \u03c9-conotoxins MVIIA and CVID in conscious rabbits and isolated tissue assays","volume":"131","author":"Wright","year":"2000","journal-title":"Br. J. Pharmacol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/S0304-3959(99)00254-7","article-title":"Adverse effects associated with the intrathecal administration of ziconotide","volume":"85","author":"Penn","year":"2000","journal-title":"Pain"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"457","DOI":"10.2174\/1568007043336743","article-title":"Voltage-gated calcium channels as targets for the treatment of chronic pain","volume":"3","author":"McGivern","year":"2004","journal-title":"Curr. Drug Targets CNS Neurol. Disord."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1254\/jphs.92.79","article-title":"Regulation of presynaptic calcium channels by synaptic proteins","volume":"92","author":"Zamponi","year":"2003","journal-title":"J. Pharmacol. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1084","DOI":"10.1592\/phco.2005.25.8.1084","article-title":"Ziconotide, an intrathecally administered N-type calcium channel antagonist for the treatment of chronic pain","volume":"25","author":"Wermeling","year":"2005","journal-title":"Pharmacotherapy"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/0304-3959(94)00094-U","article-title":"Effect of continuous intrathecal infusion of \u03c9-conopeptides, N-type calcium-channel blockers, on behavior and antinociception in the formalin and hot-plate tests in rats","volume":"60","author":"Malmberg","year":"1995","journal-title":"Pain"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/S0014-2999(02)02247-1","article-title":"Actions of intrathecal omega-conotoxins CVID, GVIA, MVIIA, and morphine in acute and neuropathic pain in the rat","volume":"451","author":"Scott","year":"2002","journal-title":"Eur. J. Pharmacol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"35335","DOI":"10.1074\/jbc.M002252200","article-title":"Novel \u03c9-conotoxins from Conus catus discriminate among neuronal calcium channel subtypes","volume":"275","author":"Lewis","year":"2000","journal-title":"J. Bol. Chem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1124\/mol.109.058834","article-title":"Analgesic \u03c9-conotoxins CVIE and CVIF selectively and voltage-dependently block recombinant and native N-type calcium channels","volume":"77","author":"Berecki","year":"2010","journal-title":"Mol. Pharmacol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1185\/03007995.2013.860019","article-title":"Incidence, patient satisfaction, and perceptions of post-surgical pain: Results from a US national survey","volume":"30","author":"Gan","year":"2014","journal-title":"Curr. Med. Res. Opin."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2287","DOI":"10.2147\/JPR.S144066","article-title":"Poorly controlled postoperative pain: Prevalence, consequences, and prevention","volume":"10","author":"Gan","year":"2017","journal-title":"J. Pain Res."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1017\/S0265021507003031","article-title":"The prevalence of postoperative pain in a sample of 1490 surgical inpatients","volume":"25","author":"Sommer","year":"2008","journal-title":"Eur. J. Anaesth."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"e00558","DOI":"10.1002\/brb3.558","article-title":"Chemotherapy-induced neuropathies-a growing problem for patients and health care providers","volume":"7","author":"Banach","year":"2017","journal-title":"Brain Behav."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"174","DOI":"10.3389\/fnmol.2017.00174","article-title":"Pathophysiology of chemotherapy-induced peripheral neuropathy","volume":"10","author":"Starobova","year":"2017","journal-title":"Front. Mol. Neurosci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1158\/1055-9965.EPI-12-1356","article-title":"Cancer survivors in the United States: prevalence across the survivorship trajectory and implications for care","volume":"22","author":"Mariotto","year":"2013","journal-title":"Cancer Epidemiol. Biomarkers Prev."},{"key":"ref_30","first-page":"1117","article-title":"Role of voltage-dependent calcium channel subtypes in experimental tactile allodynia","volume":"269","author":"Chaplan","year":"1994","journal-title":"J. Pharmacol. Exp. Therap."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/S0304-3959(99)00214-6","article-title":"Interactions of intrathecally administered ziconotide, a selective blocker of neuronal N-type voltage-sensitive calcium channels, with morphine on nociception in rats","volume":"84","author":"Wang","year":"2000","journal-title":"Pain"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1001\/jama.291.1.63","article-title":"Intrathecal ziconotide in the treatment of refractory pain in patients with cancer or AIDS: A randomized controlled trial","volume":"291","author":"Staats","year":"2004","journal-title":"J. Am. Med. Assoc."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/S0006-8993(98)00539-3","article-title":"Effect of subcutaneous administration of calcium channel blockers on nerve injury-induced hyperalgesia","volume":"801","author":"White","year":"1998","journal-title":"Brain Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1766","DOI":"10.1097\/j.pain.0000000000001567","article-title":"Antiallodynic effects of the selective NaV1.7 inhibitor Pn3a in a mouse model of acute postsurgical pain: Evidence for analgesic synergy with opioids and baclofen","volume":"160","author":"Mueller","year":"2019","journal-title":"Pain"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/S1058-9139(05)80269-4","article-title":"Thermal and mechanical hyperalgesia: A distinct role for different excitatory amino acid receptors and signal transduction pathways?","volume":"3","author":"Meller","year":"1994","journal-title":"APS J."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1068","DOI":"10.1097\/ALN.0000000000000364","article-title":"Calcitonin gene-related peptide is involved in inflammatory pain but not in postoperative pain","volume":"121","author":"Ishida","year":"2014","journal-title":"Anesthesiology"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1097\/ALN.0b013e31825a2a2b","article-title":"Gene expression in skin, muscle, and dorsal root ganglion after plantar incision in the rat","volume":"117","author":"Spofford","year":"2012","journal-title":"Anesthesiology"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1016\/j.jpain.2009.01.003","article-title":"Differential effect of capsaicin treatment on pain-related behaviors after plantar incision","volume":"10","author":"Hamalainen","year":"2009","journal-title":"J. Pain"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1744","DOI":"10.1186\/1744-8069-9-9","article-title":"TRPV1, but not TRPA1, in primary sensory neurons contributes to cutaneous incision-mediated hypersensitivity","volume":"9","author":"Barabas","year":"2013","journal-title":"Mol. Pain"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1186\/1744-8069-10-67","article-title":"TRPV1 antagonist attenuates postoperative hypersensitivity by central and peripheral mechanisms","volume":"10","author":"Uchytilova","year":"2014","journal-title":"Mol. Pain"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1109","DOI":"10.1097\/00001622-199012000-00014","article-title":"Alkylating and platinum-based agents","volume":"2","author":"Eastman","year":"1990","journal-title":"Curr. Opin. Oncol."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Schrauzer, G.N. (1977). Noble metal complexes in cancer chemotherapy. Inorganic and Nutritional Aspects of Cancer, Springer.","DOI":"10.1016\/S0006-3061(00)81035-2"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1055","DOI":"10.1097\/00001622-199112000-00014","article-title":"Alkylating agents and platinum: Is clinical resistance simply a tumor cell phenomenon?","volume":"3","author":"Reed","year":"1991","journal-title":"Curr. Opin. Oncol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1016\/j.jpain.2019.06.008","article-title":"Inflammatory and neuropathic gene expression signatures of chemotherapy-induced neuropathy induced by vincristine, cisplatin, and oxaliplatin in C57BL\/6J mice","volume":"21","author":"Starobova","year":"2020","journal-title":"J. Pain"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"534","DOI":"10.1016\/j.jpain.2008.12.003","article-title":"Comparison of oxaliplatin- and cisplatin-induced painful peripheral neuropathy in the rat","volume":"10","author":"Joseph","year":"2009","journal-title":"J. Pain"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1345\/aph.1E319","article-title":"Oxaliplatin-associated neuropathy: a review","volume":"39","author":"Cersosimo","year":"2005","journal-title":"Ann. Pharmacother."},{"key":"ref_47","first-page":"13","article-title":"Pharmacokinetics and safety profile of oxaliplatin","volume":"25","author":"Extra","year":"1998","journal-title":"Semin. Oncol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1749","DOI":"10.1016\/j.pain.2013.05.032","article-title":"An animal model of oxaliplatin-induced cold allodynia reveals a crucial role for NaV1.6 in peripheral pain pathways","volume":"154","author":"Deuis","year":"2013","journal-title":"Pain"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1744","DOI":"10.1186\/1744-8069-9-51","article-title":"Novel \u03c9-conotoxins from C. catus reverse signs of mouse inflammatory pain after systemic administration","volume":"9","author":"Sadeghi","year":"2013","journal-title":"Mol. Pain"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"2366","DOI":"10.1523\/JNEUROSCI.3828-07.2008","article-title":"Neurite outgrowth and in vivo sensory innervation mediated by a CaV2.2-laminin \u03b22 stop signal","volume":"28","author":"Sann","year":"2008","journal-title":"J. Neurosci."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Barzan, R., Pfeiffer, F., and Kukley, M. (2016). N- and L-type voltage-gated calcium channels mediate fast calcium transients in axonal shafts of mouse peripheral nerve. Front. Cell. Neurosci.","DOI":"10.3389\/fncel.2016.00135"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1016\/0304-3940(94)91105-3","article-title":"Catecholamine-induced mechanical sensitization of cutaneous nociceptors in the rat","volume":"175","author":"Gold","year":"1994","journal-title":"Neurosci. Lett."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1016\/0306-4522(90)90288-F","article-title":"Characterization of distinct phospholipases mediating bradykinin and noradrenaline hyperalgesia","volume":"39","author":"Taiwo","year":"1990","journal-title":"Neuroscience"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"4057","DOI":"10.1074\/jbc.M209969200","article-title":"\u03c9-Conotoxin CVID inhibits a pharmacologically distinct voltage-sensitive calcium channel associated with transmitter release from preganglionic nerve terminals","volume":"278","author":"Adams","year":"2003","journal-title":"J. Biol. Chem."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"34705","DOI":"10.1074\/jbc.M310848200","article-title":"The \u03b12\u03b4 auxiliary subunit reduces affinity of \u03c9-conotoxins for recombinant N-type (Cav2.2) calcium channels","volume":"279","author":"Mould","year":"2004","journal-title":"J. Biol. Chem."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0169-328X(01)00188-7","article-title":"Dorsal root ganglion neurons show increased expression of the calcium channel \u03b12\u03b4 subunit following partial sciatic nerve injury","volume":"95","author":"Newton","year":"2001","journal-title":"Brain Res. Mol. Brain Res."},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Costigan, M., Befort, K., Karchewski, L., Griffin, R.S., D'Urso, D., Allchorne, A., Sitarski, J., Mannion, J.W., Pratt, R.E., and Woolf, C.J. (2002). Replicate high-density rat genome oligonucleotide microarrays reveal hundreds of regulated genes in the dorsal root ganglion after peripheral nerve injury. BMC Neurosci., 3.","DOI":"10.1186\/1471-2202-3-16"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"8494","DOI":"10.1523\/JNEUROSCI.2982-04.2004","article-title":"Spinal dorsal horn calcium channel \u03b12\u03b41 subunit upregulation contributes to peripheral nerve injury-induced tactile allodynia","volume":"24","author":"Li","year":"2004","journal-title":"J. Neurosci."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"4076","DOI":"10.1523\/JNEUROSCI.0356-09.2009","article-title":"The increased trafficking of the calcium channel subunit \u03b12\u03b4 to presynaptic terminals in neuropathic pain is inhibited by the \u03b12\u03b4 ligand pregabalin","volume":"29","author":"Bauer","year":"2009","journal-title":"J. Neurosci."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"3287","DOI":"10.1016\/S0021-9258(18)49986-3","article-title":"Structural characterization of the dihydropyridine-sensitive calcium channel \u03b12\u03b4 subunit and the associated \u03b4 peptides","volume":"266","author":"Jay","year":"1991","journal-title":"J. Biol. Chem."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1038\/372272a0","article-title":"Structural determinants of the blockade of N-type calcium channels by a peptide neurotoxin","volume":"372","author":"Ellinor","year":"1994","journal-title":"Nature"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"20171","DOI":"10.1074\/jbc.M300581200","article-title":"Determinants of inhibition of transiently expressed voltage-gated calcium channels by \u03c9-conotoxins GVIA and MVIIA","volume":"278","author":"Feng","year":"2003","journal-title":"J. Biol. Chem."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1152\/jn.91064.2008","article-title":"\u03c9-Conotoxin GVIA alters gating charge movement of N-type (CaV2.2) calcium channels","volume":"101","author":"Yarotskyy","year":"2009","journal-title":"J. Neurophysiol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1111\/cbdd.12055","article-title":"The Future of peptide-based drugs","volume":"81","author":"Craik","year":"2013","journal-title":"Chem. Biol. Drug Des."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"99","DOI":"10.2174\/138161207779313795","article-title":"Controlled delivery of peptides and proteins","volume":"13","author":"Degim","year":"2007","journal-title":"Curr. Pharm. Des."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"1324","DOI":"10.1093\/neuonc\/nou048","article-title":"Analgesic effects of clinically used compounds in novel mouse models of polyneuropathy induced by oxaliplatin and cisplatin","volume":"16","author":"Deuis","year":"2014","journal-title":"Neuro-Oncology"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"284","DOI":"10.3389\/fnmol.2017.00284","article-title":"Methods used to evaluate pain behaviors in rodents","volume":"10","author":"Deuis","year":"2017","journal-title":"Front. Mol. Neurosci."},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Deuis, J.R., Wingerd, J.S., Winter, Z., Durek, T., Dekan, Z., Sousa, S.R., Zimmermann, K., Hoffmann, T., Weidner, C., and Nassar, M.A. (2016). Analgesic effects of GpTx-1, PF-04856264 and CNV1014802 in a mouse model of NaV1.7-mediated pain. Toxins, 8.","DOI":"10.3390\/toxins8030078"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1038\/nrn.2016.9","article-title":"Decoding the organization of spinal circuits that control locomotion","volume":"17","author":"Kiehn","year":"2016","journal-title":"Nat. Rev. Neurosci."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1080\/23328940.2016.1157668","article-title":"The thermal probe test: A novel behavioral assay to quantify thermal paw withdrawal thresholds in mice","volume":"3","author":"Deuis","year":"2016","journal-title":"Temperature"}],"container-title":["Marine Drugs"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1660-3397\/19\/2\/106\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T05:23:00Z","timestamp":1760160180000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1660-3397\/19\/2\/106"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,2,11]]},"references-count":70,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2021,2]]}},"alternative-id":["md19020106"],"URL":"https:\/\/doi.org\/10.3390\/md19020106","relation":{},"ISSN":["1660-3397"],"issn-type":[{"value":"1660-3397","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,2,11]]}}}