{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T10:57:41Z","timestamp":1787828261215,"version":"build-2784847793"},"reference-count":58,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2021,3,23]],"date-time":"2021-03-23T00:00:00Z","timestamp":1616457600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Natural Science Foundation of China;the Natural Science Foundation of Guangxi Province","award":["Natural Science Foundation of China (51372043 and 21766003), the Natural Science Foundation of Guangxi Province ( 2019GXNSFBA245098, 2017GXNSFDA198052 and 2017GXNSFBA198215)."],"award-info":[{"award-number":["Natural Science Foundation of China (51372043 and 21766003), the Natural Science Foundation of Guangxi Province ( 2019GXNSFBA245098, 2017GXNSFDA198052 and 2017GXNSFBA198215)."]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>Angiotensin-I-converting enzyme (ACE) inhibitory peptides derived from marine organism have shown a blood pressure lowering effect with no side effects. A new affinity medium of Fe3O4@ZIF-90 immobilized ACE (Fe3O4@ZIF-90-ACE) was prepared and used in the purification of ACE inhibitory peptides from Wakame (Undaria pinnatifida) protein hydrolysate (&lt;5 kDa). The Fe3O4@ZIF-90 nanoparticles were prepared by a one-pot synthesis and crude ACE extract from pig lung was immobilized onto it, which exhibited excellent stability and reusability. A novel ACE inhibitory peptide, KNFL (inhibitory concentration 50, IC50 = 225.87 \u03bcM) was identified by affinity purification using Fe3O4@ZIF-90-ACE combined with reverse phase-high performance liquid chromatography (RP-HPLC) and MALDI-TOF mass spectrometry. Lineweaver\u2013Burk analysis confirmed the non-competitive inhibition pattern of KNFL, and molecular docking showed that it bound at a non-active site of ACE via hydrogen bonds. This demonstrates that affinity purification using Fe3O4@ZIF-90-ACE is a highly efficient method for separating ACE inhibitory peptides from complex protein mixtures and the purified peptide KNFL could be developed as a functional food ingredients against hypertension.<\/jats:p>","DOI":"10.3390\/md19030177","type":"journal-article","created":{"date-parts":[[2021,3,23]],"date-time":"2021-03-23T14:08:16Z","timestamp":1616508496000},"page":"177","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Affinity Purification of Angiotensin Converting Enzyme Inhibitory Peptides from Wakame (Undaria Pinnatifida) Using Immobilized ACE on Magnetic Metal Organic Frameworks"],"prefix":"10.3390","volume":"19","author":[{"given":"Xuezhen","family":"Feng","sequence":"first","affiliation":[{"name":"Guangxi Key Laboratory of Petrochemical Resource Processing and Process Intensification Technology, School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China"},{"name":"Medical College, Guangxi University of Science and Technology, Liuzhou 545006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dankui","family":"Liao","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Petrochemical Resource Processing and Process Intensification Technology, School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lixia","family":"Sun","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Petrochemical Resource Processing and Process Intensification Technology, School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanguang","family":"Wu","sequence":"additional","affiliation":[{"name":"Medical College, Guangxi University of Science and Technology, Liuzhou 545006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Lan","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Polysaccharide Materials and Modifications, School of Chemistry and Chemical Engineering, Guangxi University for Nationalities, Nanning 530008, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zefen","family":"Wang","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Petrochemical Resource Processing and Process Intensification Technology, School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunzhi","family":"Li","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Petrochemical Resource Processing and Process Intensification Technology, School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Zhou","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Petrochemical Resource Processing and Process Intensification Technology, School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Lu","sequence":"additional","affiliation":[{"name":"Medical College, Guangxi University of Science and Technology, Liuzhou 545006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiongdiao","family":"Lan","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Polysaccharide Materials and Modifications, School of Chemistry and Chemical Engineering, Guangxi University for Nationalities, Nanning 530008, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,3,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"8106","DOI":"10.1021\/jf0508908","article-title":"ACE inhibitory peptides derived from enzymatic hydrolysates of animal muscle protein: A review","volume":"53","author":"Vercruysse","year":"2005","journal-title":"J. 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