{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T17:47:37Z","timestamp":1770140857955,"version":"3.49.0"},"reference-count":46,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2021,4,15]],"date-time":"2021-04-15T00:00:00Z","timestamp":1618444800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100017052","name":"NSFC-Liaoning Joint Fund","doi-asserted-by":"publisher","award":["No. 81502955"],"award-info":[{"award-number":["No. 81502955"]}],"id":[{"id":"10.13039\/100017052","id-type":"DOI","asserted-by":"publisher"}]},{"name":"the Doctoral Scientific Research Foundation of Shanghai Ocean University","award":["No. A2030214300077"],"award-info":[{"award-number":["No. A2030214300077"]}]},{"name":"the Young Teachers Training Program of Shanghai","award":["No. A12056160002"],"award-info":[{"award-number":["No. A12056160002"]}]},{"name":"the Plan of Innovation Action in Shanghai","award":["No. 14431906000"],"award-info":[{"award-number":["No. 14431906000"]}]},{"name":"Ruilong Sheng","award":["M1420-01-0145-FEDER-000005-Centro de Qu\u00edmica da Madeira-CQM+(Madeira 14-20)"],"award-info":[{"award-number":["M1420-01-0145-FEDER-000005-Centro de Qu\u00edmica da Madeira-CQM+(Madeira 14-20)"]}]},{"name":"Ruilong Sheng","award":["ARDITI-2017-ISG-00"],"award-info":[{"award-number":["ARDITI-2017-ISG-00"]}]},{"name":"Portuguese Government funds","award":["project PEst-OE\/QUI\/UI0674\/2019, CQM"],"award-info":[{"award-number":["project PEst-OE\/QUI\/UI0674\/2019, CQM"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>2,5-Bis-[8-(4,8-dimethyl-nona-3,7-dienyl)-5,7-dihydroxy-8-methyl-3-keto-1,2,7,8-teraahydro-6H-pyran[a]isoindol-2-yl]-pentanoic acid (FGFC1) is a marine pyran-isoindolone derivative isolated from a rare marine microorganism Stachybotrys longispora FG216, which showed moderate antithrombotic(fibrinolytic) activity. To further enhance its antithrombotic effect, a series of new FGFC1 derivatives (F1\u2013F7) were synthesized via chemical modification at C-2 and C-2\u2032 phenol groups moieties and C-1\u2033 carboxyl group. Their fibrinolytic activities in vitro were evaluated. Among the derivatives, F1\u2013F4 and F6 showed significant fibrinolytic activities with EC50 of 59.7, 87.1, 66.6, 82.8, and 42.3 \u03bcM, respectively, via enhancement of urokinase activity. Notably, derivative F6 presented the most remarkable fibrinolytic activity (2.72-fold than that of FGFC1). Furthermore, the cytotoxicity of derivative F6 was tested as well as expression of Fas\/Apo-1 and IL-1 on HeLa cells. The results showed that, compared to FGFC1, derivative F6 possessed moderate cytotoxicity and apoptotic effect on HeLa cells (statistical significance p &gt; 0.1), making F6 a potential antithrombotic agent towards clinical application.<\/jats:p>","DOI":"10.3390\/md19040218","type":"journal-article","created":{"date-parts":[[2021,4,15]],"date-time":"2021-04-15T21:35:13Z","timestamp":1618522513000},"page":"218","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Synthesis and Bioactivities of Marine Pyran-Isoindolone Derivatives as Potential Antithrombotic Agents"],"prefix":"10.3390","volume":"19","author":[{"given":"Yinan","family":"Wang","sequence":"first","affiliation":[{"name":"College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Chen","sequence":"additional","affiliation":[{"name":"Shanghai Engineering Research Center of Hadal Science and Technology, College of Marine Sciences, Shanghai Ocean University, Shanghai 201306, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3303-3832","authenticated-orcid":false,"given":"Ruilong","family":"Sheng","sequence":"additional","affiliation":[{"name":"CQM-Centro de Qu\u00edmica da Madeira, Campus da Penteada, Universidade da Madeira, 9000-390 Funchal, Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhe","family":"Fu","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junting","family":"Fan","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Nanjing Medical University, Nanjing 211166, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1710-8582","authenticated-orcid":false,"given":"Wenhui","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qidong","family":"Tu","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Jiangxi Science and Technology Normal University, Nanchang 330013, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8414-886X","authenticated-orcid":false,"given":"Ruihua","family":"Guo","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China"},{"name":"Shanghai Engineering Research Center of Aquatic-Product Processing &amp; Preservation, Shanghai 201306, China"},{"name":"Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture, Shanghai 201306, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,4,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1002\/ped4.12254","article-title":"Noncommunicable chronic disease prevention should start from childhood","volume":"5","author":"Yan","year":"2021","journal-title":"Pediatric. 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